Semejanza externa vs. semejanza sinapomórfica: el caso de Palaeomerycidae y Dromomerycidae (Cetartiodactyla, Ruminantia, Pecora)

Comunicación presentada en el XIII Encuentro en Jóvenes Investigadores en Paleontología (XIII EJIP) - XIII Meeting of Early-Stage Researchers in Paleontology (XIII EJIP): Cercedilla, 15 - 18 de Abril de 2015Peer reviewe

Design of the Front End Electronics for the Infrared Camera of JEM-EUSO, and manufacturing and verification of the prototype model

The Japanese Experiment Module (JEM) Extreme Universe Space Observatory (EUSO) will be launched and attached to the Japanese module of the International Space Station (ISS). Its aim is to observe UV photon tracks produced by ultra-high energy cosmic rays developing in the atmosphere and producing extensive air showers. The key element of the instrument is a very wide-field, very fast, large-lense telescope that can detect extreme energy particles with energy above $10^{19}$ eV. The Atmospheric Monitoring System (AMS), comprising, among others, the Infrared Camera (IRCAM), which is the Spanish contribution, plays a fundamental role in the understanding of the atmospheric conditions in the Field of View (FoV) of the telescope. It is used to detect the temperature of clouds and to obtain the cloud coverage and cloud top altitude during the observation period of the JEM-EUSO main instrument. SENER is responsible for the preliminary design of the Front End Electronics (FEE) of the Infrared Camera, based on an uncooled microbolometer, and the manufacturing and verification of the prototype model. This paper describes the flight design drivers and key factors to achieve the target features, namely, detector biasing with electrical noise better than $100 \mu$V from $1$ Hz to $10$ MHz, temperature control of the microbolometer, from $10^{\circ}$C to $40^{\circ}$C with stability better than $10$ mK over $4.8$ hours, low noise high bandwidth amplifier adaptation of the microbolometer output to differential input before analog to digital conversion, housekeeping generation, microbolometer control, and image accumulation for noise reduction

Alpha-catenin-Dependent Recruitment of the Centrosomal Protein CAP350 to Adherens Junctions Allows Epithelial Cells to Acquire a Columnar Shape

Epithelial morphogenesis involves a dramatic reorganisation of the microtubule cytoskeleton. How this complex process is controlled at the molecular level is still largely unknown. Here, we report that the centrosomal microtubule (MT)-binding protein CAP350 localises at adherens junctions in epithelial cells. By two-hybrid screening, we identified a direct interaction of CAP350 with the adhesion protein α-catenin that was further confirmed by co-immunoprecipitation experiments. Block of epithelial cadherin (E-cadherin)-mediated cell-cell adhesion or α-catenin depletion prevented CAP350 localisation at cell-cell junctions. Knocking down junction-located CAP350 inhibited the establishment of an apico-basal array of microtubules and impaired the acquisition of columnar shape in Madin-Darby canine kidney II (MDCKII) cells grown as polarised epithelia. Furthermore, MDCKII cystogenesis was also defective in junctional CAP350-depleted cells. CAP350-depleted MDCKII cysts were smaller and contained either multiple lumens or no lumen. Membrane polarity was not affected, but cortical microtubule bundles did not properly form. Our results indicate that CAP350 may act as an adaptor between adherens junctions and microtubules, thus regulating epithelial differentiation and contributing to the definition of cell architecture. We also uncover a central role of α-catenin in global cytoskeleton remodelling, in which it acts not only on actin but also on MT reorganisation during epithelial morphogenesis.This work was supported by Ministerio de Economia y Competitividad, Spain (BFU2012-36717 and CSD2009-00016 to RMR and BFU2011-22916 to JRM) and by Junta de Andalucia (CVI-7256 and CTS-2071), and by a funding GenHomme Network 02490-6088 to Hybrigenics and the Institut Curie. MA and AZ were supported by MEC–FPI Grants.Peer Reviewe

Uso de viruta de madera y lirio acuático (eichhornia crassipes) como agentes estructurantes en tratamientos aerobios de residuos hortícolas.

En procesos aerobios para tratar residuos orgánicos con alto contenido de humedad y bajo contenido de lignina y celulosa, deben emplearse agentes estructurantes para favorecer la aireación y por lo tanto el buen desarrollo del bioproceso. En este trabajo se utilizó una mezcla de viruta de madera y lirio acuático seco (Eichhornia crassipes) como agente estructurante en diferentes porcentajes (5, 10, 15 y 20 %). Cada tratamiento fue hecho por triplicado y estuvo compuesto de una mezcla que contenía los seis bioresiduos generados en mayor cantidad en la Central de Abasto de la Ciudad de México. Los experimentos se llevaron a cabo en celdas de material acrílico donde se colocó una mezcla de 1200 g de residuos y material estructurante. Durante el proceso, los parámetros medidos fueron el pH, la temperatura, la densidad, el contenido de humedad, la generación de lixiviados y la reducción de masa. Los resultados mostraron que el elevado contenido de humedad de la mezcla puede ser regulado con materiales estructurantes secos, ya que estos materiales absorben parte del lixiviado durante las primeras semanas del proceso y mejoran la textura de las pilas evitando con esto que la degradación se lleve a cabo en condiciones de anaerobiosis. Tomando como base la generación de lixiviados y la reducción de masa, los mejores resultados se obtuvieron usando un 15 % de agente estructurante, el cual estaba compuesto de una mezcla de 6:1 viruta de madera y lirio acuático.In aerobic treatments for solid waste with high humidity content and low lignine and cellulose, bulking agents must be applied in order to improve the aeration and the performance of the process. In the present work, a mixture of wood shaving and dry water lily (Eichhornia crassipes) was tested as bulking agent at different percentages (5, 10, 15 and 20 %). Each assay was prepared by triplicate and was composed by a mixture of the six most important horticultural waste from a big market (Central de Abasto de la Ciudad de Mexico). The assays were run in acrylic cells where 1200 g of organic waste and bulking agent were mixed. During the process, pH, temperature, density, humidity content, leachate generation and mass reduction were measured. Results showed that the high rate of humidity of this mixture could be regulated by adding dry materials. These agents absorb some leachate during the first weeks of the process and the texture in the windrow was improved avoiding the anaerobic biodegradation of this kind of waste. Evaluating the leachates generation and the mass reduction, the best results have been obtained using 15 % of bulking agent, which it was composed by 6:1 wood shavings and dry water lily.Los autores agradecen al Consejo Nacional de Ciencia y Tecnología (CONACYT) y al Instituto Politécnico Nacional (IPN) por el financiamiento de este proyecto y a la COFAA-IPN por el apoyo para la divulgación de los resultados de este trabajo

Evidence of suppression of onchocerciasis transmission in the Venezuelan Amazonian focus.

BACKGROUND: The World Health Organization (WHO) has set goals for onchocerciasis elimination in Latin America by 2015. Most of the six previously endemic countries are attaining this goal by implementing twice a year (and in some foci, quarterly) mass ivermectin (Mectizan®) distribution. Elimination of transmission has been verified in Colombia, Ecuador and Mexico. Challenges remain in the Amazonian focus straddling Venezuela and Brazil, where the disease affects the hard-to-reach Yanomami indigenous population. We provide evidence of suppression of Onchocerca volvulus transmission by Simulium guianense s.l. in 16 previously hyperendemic Yanomami communities in southern Venezuela after 15 years of 6-monthly and 5 years of 3-monthly mass ivermectin treatment. METHODS: Baseline and monitoring and evaluation parasitological, ophthalmological, entomological and serological surveys were conducted in selected sentinel and extra-sentinel communities of the focus throughout the implementation of the programme. RESULTS: From 2010 to 2012–2015, clinico-parasitological surveys indicate a substantial decrease in skin microfilarial prevalence and intensity of infection; accompanied by no evidence (or very low prevalence and intensity) of ocular microfilariae in the examined population. Of a total of 51,341 S. guianense flies tested by PCR none had L3 infection (heads only). Prevalence of infective flies and seasonal transmission potentials in 2012–2013 were, respectively, under 1 % and 20 L3/person/transmission season. Serology in children aged 1–10 years demonstrated that although 26 out of 396 (7 %) individuals still had Ov-16 antibodies, only 4/218 (2 %) seropositives were aged 1–5 years. CONCLUSIONS: We report evidence of recent transmission and morbidity suppression in some communities of the focus representing 75 % of the Yanomami population and 70 % of all known communities. We conclude that onchocerciasis transmission could be feasibly interrupted in the Venezuelan Amazonian focus. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1313-z) contains supplementary material, which is available to authorized users

Efficacy of novel indoor residual spraying methods targeting pyrethroid-resistant aedes aegypti within experimental houses

Challenges in maintaining high effectiveness of classic vector control in urban areas has renewed the interest in indoor residual spraying (IRS) as a promising approach for Aedes-borne disease prevention. While IRS has many benefits, application time and intrusive indoor applications make its scalability in urban areas difficult. Modifying IRS to account for Ae. aegypti resting behavior, named targeted IRS (TIRS, spraying walls below 1.5 m and under furniture) can reduce application time; however, an untested assumption is that modifications to IRS will not negatively impact entomological efficacy. We conducted a comparative experimental study evaluating the residual efficacy of classically-applied IRS (as developed for malaria control) compared to two TIRS application methods using a carbamate insecticide against a pyrethroid-resistant, field-derived Ae. aegypti strain. We performed our study within a novel experimental house setting (n = 9 houses) located in Merida (Mexico), with similar layouts and standardized contents. Classic IRS application (insecti-cide applied to full walls and under furniture) was compared to: a) TIRS: insecticide applied to walls below 1.5 m and under furniture, and b) Resting Site TIRS (RS-TIRS): insecticide applied only under furniture. Mosquito mortality was measured eight times post-application (out to six months post-application) by releasing 100 Ae. aegypti females/house and collecting live and dead individuals after 24 hrs exposure. Compared to Classic IRS, TIRS and RS-TIRS took less time to apply (31% and 82% reduction, respectively) and used less insecticide (38% and 85% reduction, respectively). Mortality of pyrethroid-resistant Ae. aegypti did not significantly differ among the three IRS application methods up to two months post application, and did not significantly differ between Classic IRS and TIRS up to four months post application. These data illustrate that optimizing IRS to more efficiently target Ae. aegypti

Evaluación del uso de los cementos de ionómetros vítreos en la práctica diaria

Los Cementos de ionómeros vítreos (CIV) son usados como biomateriales por sus amplias aplicaciones clínicas y múltiples ventajas. Los objetivos de este trabajo fueron determinar si los profesionales utilizan o no en la práctica diaria los cementos de ionómeros vítreos y tratar de identificar el porqué, como así también validar o innovar la metodología en la enseñanza de este material, en la Cátedra de Operatoria I “B”. Materiales y Métodos: Los datos se obtuvieron de un cuestionario anónimo autoadministrado, con preguntas cerradas en una muestra aleatoria de 148 odontólogos, con un período promedio de diez años de egresado, pertenecientes al padrón de prestadores del Círculo Odontológico de Córdoba. Resultados: El 80,41 % de los encuestados afirmaron que utilizan el CIV. El 19,59 % que no lo usa opina que es costoso y que algunas obras sociales no lo reconocen (89,66%). El 56,08 % refiere que los aplica como base o relleno y que prefiere como mecanismo de endurecimiento la fotopolimerización (56,76%). Al valorar las ventajas el 53,38 % destacó la liberación de fluoruro y propiedades físico-mecánicas similares a la dentina. Como desventaja el 54,73 % destacó la necesidad de proporcionarlo adecuadamente y el costo elevado. Para la compra del producto, el criterio de selección es el consejo del vendedor y el costo material (54,73%). En conclusión es utilizado por un alto porcentaje de odontólogos generalistas, de lo que se deduce que fue adecuada la incorporación de este contenido en la enseñanza preclínica de gradopublishedVersio

Influence of the ripening chamber's geographical location on dry-cured Iberian ham's key odorants

Olfactometric and sensory analyses have been applied to study the possible influence of the ripening chamber’s geographical location on the aroma sensory profiles and key odorants of Iberian ham. Dry-cured Iberian ham was obtained from 3 acorn-fed pigs and, for the first time, both of the participating production facilities, located in two different Andalusian municipalities with different altitudes above mean sea level, processed one of the two hind legs from each pig. The descriptive sensory profile of orthonasal and retronasal odours was determined by trained panellists, while odour-active compounds were determined by gas chromatography/mass spectrometry-olfactometry (GC/MS-O). The results obtained showed that, separately, both techniques enable Iberian ham samples to be differentiated by their ripening chamber’s geographical location. For sensory analysis, retronasal sensory analysis appeared to be the most suitable for this goal, highlighting the “meat broth odour” and “roasted nuts odour” descriptors which presented significant differences between geographical locations for samples from all pigs. Moreover, ripening chamber’s geographical location characteristics and the initial composition of the raw material seemed to influence the content of some odour-active compounds. The odour-active compound identified as octane/acetone and isobutanol were conditioned by the ripening chamber’s geographical location, while decanal/2-ethyl-1-hexanol, 1-undecanol, 2-furanmethanol and cis-2-nonenal were also influenced by the individual pig itself. This study showed that slight climatological differences due to the location of the ripening chamber seem to have somewhat of an influence on the aromatic profile.Universidad de Sevilla VIPPIT-2019-I

P9 23. Seudoaneurisma aórtico con infeción de prótesis en aorta ascendente. ¿Es necesario retirar siempre la prótesis? ¿Cuánto tiempo con tratamiento antibiótico?

IntroducciónEl seudoaneurisma aórtico con infección de prótesis en aorta ascendente (PIPAA) tras cirugía cardíaca es una entidad infrecuente (0,9-2%) pero grave (mortalidad intrahospitalaria > 40%). El tratamiento más extendido es la cirugía con recambio protésico y terapia antibiótica adecuada a antibiograma; pero el recambio protésico en ocasiones es técnicamente inviable e incluso puede aumentar la mortalidad perioperatoria. Existen casos en los que se ha preservado la prótesis infectada con éxito terapéutico, realizándose limpieza/reparación quirúrgica local apoyada con omentoplastia. No existe consenso en la duración de la terapia médica, y el tratamiento «supresor a largo plazo» en ocasiones se complica por efectos adversos de los antibióticos.ObjetivosAportar dos nuevos casos y evaluar el tratamiento realizado tras un seguimiento a largo plazo.MétodoAnálisis descriptivo de aspectos microbiológicos, farmacológicos y resultados de la terapia realizada, en dos casos de PIPAA de pacientes intervenidos por disección de aorta (prótesis de dacrón en posición supracoronariana) y por insuficiencia y anuloectasia aórtica (tubo valvulado). Se realiza tratamiento quirúrgico conservador de la prótesis aórtica (limpieza quirúrgica, reparación del seudoaneurisma y omentoplastia), asociándose terapia antibiótica prolongada ajustada a antibiograma.ConclusiónAmbos casos presentan, tras más de 1 año de seguimiento, según criterios clínicos, microbiológicos y pruebas de imagen, ausencia de signos de recidiva infecciosa, resultando la terapia adecuada. Aun sin poder establecer tiempo óptimo de tratamiento, serían razonables 6 semanas de tratamiento endovenoso seguidas de 24 semanas de terapia supresora, a ser posible oral, y valorar su retirada siempre que no existan signos de recidiva