137 research outputs found
Characterization of Salmonella species from pork meat in Tolima, Colombia
Background: Salmonella is a Gram-negative bacterium and the principal cause of human gastroenteritis that originates from the consumption of animal products.Objective: to determine serotype and antibiotic resistance of Salmonella spp. isolated from pork meat and environmental samples in 6 slaughterhouses and 14 butcheries in Tolima, Colombia. Methods: slaughterhouses and butcheries were selected depending on their slaughter capacity and compliance with good manufacturing practices. Samples (n = 507) were taken from carcasses, the environment, and fomites (i.e., surfaces of knives, hooks, floor, siphons, work surfaces, and transport trucks), then cultured in Salmonella selective media. Following this, the isolated Salmonella spp. was identified using a conventional biochemical test and genus antiserum (Poli A + Vi). The Kauffman-Whyte scheme was used for serotyping and the agar diffusion method (Kirby-Bauer) was used to determine antibiotic sensitivity. Results:Manhattan, Derby, Typhimurium, Javiana, Muenster, Hvittingfoss, Sinsfort, Kattbus, and Saint Paul serotypes of Salmonella were isolated from both pork meat and environmental samples, being Derby the most common serotype. Salmonella isolates showed antibiotic multiresistance mainly to tetracycline, lincomycin and nalidixic acid. Conclusion: several Salmonella serotypes are present in slaughterhouses and meat samples from butcheries, and they show similar antibiotic resistance patterns. This work represents the first report on Salmonella serotypes in slaughterhouses and pork meat from butcheries in Tolima, Colombia.Antecedentes: a Salmonella é uma bactéria Gram-negativa e causa principal de gastroenterite humana transmitida por consumo de produtos de origem animal. Objetivo: determinar o serotipo e de resistência a antibióticos de Salmonella spp. isolada a partir de carne de porco e amostras ambientais em 6 frigoríficos e lojas 14 açougues em Tolima, Colômbia. Métodos: abatedouros e açougues foram selecionados de acordo com o volume de animais sacrificados eo estabelecimento de boas práticas de manufatura. As amostras (n = 507) foram retirados de cadáveres, meio ambiente e fômites (ou seja, superfícies de facas, ganchos, piso, sifões, mésons e caminhões de transporte), cultivadas em meios de cultura de Salmonella seletiva e a isolada Salmonella spp., foram identificados usando e anti-soro de teste género bioquímica convencional (Poli A + Vi). A sorotipagem foi realizada utilizando esquema de Kauffman-Whyte e teste de sensibilidade aos antibióticos foi feito pelo método de difusão em ágar (Kirby-Bauer). Resultados: ''o'' sorotipos de Salmonella de Manhattan,= o surotipos de Salmonella Derby, Typhimurium, Javiana, Muenster, Hvittingfoss, Sinsfort, Kattbus e Saint Paul foram isolados de ambas as carnes de porco e amostras ambientais, sendo o sorotipo Derby o mais frequente. Os isolados de Salmonella apresentaram padrões de multirresistência aos antibióticos, principalmente para tetraciclina, lincomicina e ácido nalidíxico. Conclusão: vários sorotipos de Salmonella estão presentes em matadouros e lojas amostras de carne de porco de talho e mostram padrões similares de resistência a antibióticos. Este trabalho representa o primeiro relato de sorotipos de Salmonella em abatedouros e açougues de carne de porco em Tolima, Colômbia.Antecedentes: Salmonella es una bacteria Gram-negativa y la principal causa de gastroenteritis en humanos transmitida por consumo de alimentos de origen animal.Objective: determinar el serotipo y la resistencia antibiótica de Salmonella spp. aisladas de carne porcina y muestras ambientales en 6 plantas de beneficio y 14 expendios en Tolima, Colombia. Métodos:las plantas de beneficio y los expendios fueron seleccionados dependiendo del volumen de animales sacrificados y el establecimiento de las buenas prácticas de manufactura. Las muestras (n = 507) fueron tomadas de las carcasas, ambiente y fómites (i.e. superficie de cuchillos, ganchos, piso, sifones, mesones y camiones de transporte), cultivadas en medios de cultivo selectivos para Salmonella. Las Salmonella spp., aisladas fueron identificadas mediante purnas bioquímicas convencionales y antisuero de género (Poli A + Vi). La sero-tipificación fue llevada a cabo a través del esquema Kauffman-Whyte y la sensibilidad antibiótica a través del método de difusión en agar (Kirby-Bauer).. Resultados: fueron aislados los serotipos de Salmonella Manhattan, Derby, Typhimurium, Javiana, Muenster, Hvittingfoss, Sinsfort, Kattbus y Saint Paul tanto de carne porcina como muestras ambientales, siendo el serotipo Derby el más frecuente. Los aislamientos mostraron patrones de multi-resistencia antibiótica, principalmente a tetraciclina, lincomicina y ácido nalidíxico. Conclusión: diversos serotipos de Salmonella fueron aislados de muestras de plantas de beneficio y expendios de carne porcina y estos demostraron patrones similares de resistencia antibiótica. Este trabajo representa el primer reporte de serotipos de Salmonella en plantas de beneficio y expendios de carne porcina en Tolima, Colombia
Prevalencia de Salmonella spp. en carne porcina, plantas de beneficio y expendios del Tolima
Salmonellosis is an infectious disease whose main clinical manifestation is acute gastroenteritis caused by consumption of contaminated water or food especially meat. The aim of this study was to determine the Salmonella spp. prevalence inside pork and the environment from 6 slaughterhouses and 14 butcher's shops all of them selected in Tolima region. A convenience study was realized, choosing slaughterhouses depending on animal reception charge and the good manufacture practices. Sample were obtained (n=507) from environment samples were realized smears on knives, tables, hooks, waste pipes, floors, and transportation trucks. Samples were under microbiologic analysis for Salmonella isolation, which was identified by biochemical and serological test (Poli A +Vi). The results showed a prevalence of Salmonella spp. of 4.93% (25/507). 14 out 25 positives (56%) were isolated from carcass and 11 remaining (44%) were isolated from environment; in slaughterhouses as well as butcher's shops. In conclusion, Isolation of Salmonella spp. in post-production phases can be due to cross contamination with environmental fomites in transport as well as in butcher's shops and it's important for the risk in public health. This is the first time, in the Tolima region that the Salmonella prevalence is known in slaughterhouses and butcher's shops.A salmonelose é uma doença infecciosa cuja manifestação clínica principal é a gastroenterite aguda causada pelo consumo de água ou alimentos, especialmente carnes contaminados. O objetivo deste estudo foi determinar a prevalência de Salmonellaspp. na carne suína e em ambientes de 6 frigoríficos e 14 açougues selecionados no departamento de Tolima. Foi desenvolvido um por conveniência, a seleção de frigoríficos foi pelo volume de sacrifício e a gestão de boas práticas de manufatura. As amostras foram obtidas (n = 507) de carcaças, ambientes e fômites (ou seja, raspagem de facas, ganchos, pisos, sifões mesas e caminhões transportadores). Estas amostras foram submetidas à análise microbiológica para o isolamento de Salmonella spp.,a qual foi identificado por testes bioquímicos e serológicos (Poli A + Vi). Os resultados mostraram uma prevalência de 4,3% (25/507) de Salmonella spp. Das 25 amostras positivas 14 (56%) delas foram isoladas de carcaças e os restantes 11 (44%) foram isolados de ambientes, tanto de frigoríficos como dos açougues. Em conclusão, a presença de Salmonella spp. emfases de pós-produção (transformação e comercialização) pode ocorrer por contaminação por fômites ambientais no transporte e nos pontos de venda e é importante devido ao risco à saúde pública. Este trabalho constitui o primeiro reporte sobre a prevalência de Salmonella spp. em plantas de processamento e açougues, no departamento de Tolima.La salmonelosis es una enfermedad infectocontagiosa cuya principal manifestación clínica es la gastroenteritis aguda, causada por consumo de agua o alimentos contaminados especialmente carnes. El objetivo del presente trabajo fue determinar de la prevalencia de Salmonella spp. en carne porcina y en ambientes de 6 plantas de beneficio y 14 expendios seleccionados en el departamento del Tolima. Para ello se desarrolló un estudio por conveniencia, seleccionando las plantas por su volumen de sacrificio y el manejo de las buenas prácticas de manufactura. Se obtuvieron muestras (n=507) de canales, ambientes y fómites (i.e. frotis de cuchillos, ganchos, pisos, sifones, mesones y camiones transportadores). Estas muestras fueron sometidas a análisis microbiológico para el aislamiento de Salmonella spp., la cual fue identificada por pruebas bioquímicas y serológicas (Poli A + Vi). Los resultados arrojaron una prevalencia del 4.3% (25/507) de Salmonella spp. De las 25 muestras positivas 14 (56%) de ellas fueron asiladas de canales y las 11 restantes (44%) se aislaron de ambientes; tanto de las plantas de beneficio como de los expendios. En conclusión la presencia de Salmonella spp. en fases de posproducción (beneficio y comercialización) puede presentarse por contaminación con fómites ambientales en el transporte así como en los expendios y reviste importancia dado el riesgo para la salud pública. Este trabajo constituye el primer reporte de prevalencia de Salmonella spp. en plantas de beneficio y expendios en el departamento del Tolima
Prevalencia de Salmonella spp. en carne porcina, plantas de beneficio y expendios del Tolima
tirulo en ingles: Salmonella spp. prevalence in pork, slaughterhouses and butcher’s shops in the Tolima department of Colombia.titulo en portugues: Prevalência de Salmonella spp. em carne suína, plantas de processamento e açougues doTolima Resumen: La salmonelosis es una enfermedad infectocontagiosa cuya principal manifestación clínica es la gastroenteritis aguda, causada por consumo de agua o alimentos contaminados especialmente carnes. El objetivo del presente trabajo fue deter- minar de la prevalencia de Salmonella spp. en carne porcina y en ambientes de 6 plantas de beneficio y 14 expendios selec- cionados en el departamento del Tolima. Para ello se desarrolló un estudio por conveniencia, seleccionando las plantas por su volumen de sacrificio y el manejo de las buenas prácticas de manufactura. Se obtuvieron muestras (n=507) de canales, ambientes y fómites (i.e. frotis de cuchillos, ganchos, pisos, sifones, mesones y camiones transportadores). Estas muestras fueron sometidas a análisis microbiológico para el aislamiento de Salmonella spp., la cual fue identificada por pruebas bioquímicas y serológicas (Poli A + Vi). Los resultados arrojaron una prevalencia del 4.3% (25/507) de Salmonella spp. De las 25 muestras positivas 14 (56%) de ellas fueron asiladas de canales y las 11 restantes (44%) se aislaron de ambientes; tanto de las plantas de beneficio como de los expendios. En conclusión la presencia de Salmonella spp. en fases de pospro- ducción (beneficio y comercialización) puede presentarse por contaminación con fómites ambientales en el transporte así como en los expendios y reviste importancia dado el riesgo para la salud pública. Este trabajo constituye el primer reporte de prevalencia de Salmonella spp. en plantas de beneficio y expendios en el departamento del Tolima.Palabras clave: plantas de beneficio, porcino, productos cárnicos, Salmonella spp.Abstract: Salmonellosis is an infectious disease whose main clinical manifestation is acute gastroenteritis caused by consumption of contaminated water or food especially meat. The aim of this study was to determine the Salmonella spp. prevalence insidepork and the environment from 6 slaughterhouses and 14 butcher’s shops all of them selected in Tolima region. A convenien- ce study was realized, choosing laughterhouses depending on animal reception charge and the good manufacture practices. Sample were obtained (n=507) from environment samples were realized smears on knives, tables, hooks, waste pipes, floors, and transportation trucks. Samples were under microbiologic analysis for Salmonella isolation, which was identified by bioche- mical and serological test (Poli A +Vi). The results showed a prevalence of Salmonella spp. of 4.93% (25/507). 14 out 25 posi- tives (56%) were isolated from carcass and 11 remaining (44%) were isolated from environment; in slaughterhouses as well as butcher’s shops. In conclusion, Isolation of Salmonella spp. in post-production phases can be due to cross contamination with environmental fomites in transport as well as in butcher’s shops and it’s important for the risk in public health. This is the first time, in the Tolima region that the Salmonella prevalence is known in slaughterhouses and butcher’s shops.Key words: abattoirs, swine, meat products, Salmonella spp.Resumo: A salmonelose é uma doença infecciosa cuja manifestação clínica principal é a gastroenterite aguda causada pelo consu- mo de água ou alimentos, especialmente carnes contaminados. O objetivo deste estudo foi determinar a prevalência de Salmonellaspp. na carne suína e em ambientes de 6 frigoríficos e 14 açougues selecionados no departamento de Tolima. Foi desenvolvido um por conveniência, a seleção de frigoríficos foi pelo volume de sacrifício e a gestão de boas práticas de manufatura. As amostras foram obtidas (n = 507) de carcaças, ambientes e fômites (ou seja, raspagem de facas, gan- chos, pisos, sifões mesas e caminhões transportadores). Estas amostras foram submetidas à análise microbiológica para o isolamento de Salmonella spp.,a qual foi identificado por testes bioquímicos e serológicos (Poli A + Vi). Os resultados mostraram uma prevalência de 4,3% (25/507) de Salmonella spp. Das 25 amostras positivas 14 (56%) delas foram isoladas de carcaças e os restantes 11 (44%) foram isolados de ambientes, tanto de frigoríficos como dos açougues. Em conclusão, a presença de Salmonella spp. emfases de pós-produção (transformação e comercialização) pode ocorrer por contami- nação por fômites ambientais no transporte e nos pontos de venda e é importante devido ao risco à saúde pública. Este trabalho constitui o primeiro reporte sobre a prevalência de Salmonella spp. em plantas de processamento e açougues, no departamento de Tolima.Palavras chave: plantas de processamento, suínos, produtos carne, Salmonella spp.tirulo en ingles: Salmonella spp. prevalence in pork, slaughterhouses and butcher’s shops in the Tolima department of Colombia.titulo en portugues: Prevalência de Salmonella spp. em carne suína, plantas de processamento e açougues doTolima Resumen: La salmonelosis es una enfermedad infectocontagiosa cuya principal manifestación clínica es la gastroenteritis aguda, causada por consumo de agua o alimentos contaminados especialmente carnes. El objetivo del presente trabajo fue deter- minar de la prevalencia de Salmonella spp. en carne porcina y en ambientes de 6 plantas de beneficio y 14 expendios selec- cionados en el departamento del Tolima. Para ello se desarrolló un estudio por conveniencia, seleccionando las plantas por su volumen de sacrificio y el manejo de las buenas prácticas de manufactura. Se obtuvieron muestras (n=507) de canales, ambientes y fómites (i.e. frotis de cuchillos, ganchos, pisos, sifones, mesones y camiones transportadores). Estas muestras fueron sometidas a análisis microbiológico para el aislamiento de Salmonella spp., la cual fue identificada por pruebas bioquímicas y serológicas (Poli A + Vi). Los resultados arrojaron una prevalencia del 4.3% (25/507) de Salmonella spp. De las 25 muestras positivas 14 (56%) de ellas fueron asiladas de canales y las 11 restantes (44%) se aislaron de ambientes; tanto de las plantas de beneficio como de los expendios. En conclusión la presencia de Salmonella spp. en fases de pospro- ducción (beneficio y comercialización) puede presentarse por contaminación con fómites ambientales en el transporte así como en los expendios y reviste importancia dado el riesgo para la salud pública. Este trabajo constituye el primer reporte de prevalencia de Salmonella spp. en plantas de beneficio y expendios en el departamento del Tolima.Palabras clave: plantas de beneficio, porcino, productos cárnicos, Salmonella spp.Abstract: Salmonellosis is an infectious disease whose main clinical manifestation is acute gastroenteritis caused by consumption of contaminated water or food especially meat. The aim of this study was to determine the Salmonella spp. prevalence insidepork and the environment from 6 slaughterhouses and 14 butcher’s shops all of them selected in Tolima region. A convenien- ce study was realized, choosing laughterhouses depending on animal reception charge and the good manufacture practices. Sample were obtained (n=507) from environment samples were realized smears on knives, tables, hooks, waste pipes, floors, and transportation trucks. Samples were under microbiologic analysis for Salmonella isolation, which was identified by bioche- mical and serological test (Poli A +Vi). The results showed a prevalence of Salmonella spp. of 4.93% (25/507). 14 out 25 posi- tives (56%) were isolated from carcass and 11 remaining (44%) were isolated from environment; in slaughterhouses as well as butcher’s shops. In conclusion, Isolation of Salmonella spp. in post-production phases can be due to cross contamination with environmental fomites in transport as well as in butcher’s shops and it’s important for the risk in public health. This is the first time, in the Tolima region that the Salmonella prevalence is known in slaughterhouses and butcher’s shops.Key words: abattoirs, swine, meat products, Salmonella spp.Resumo: A salmonelose é uma doença infecciosa cuja manifestação clínica principal é a gastroenterite aguda causada pelo consu- mo de água ou alimentos, especialmente carnes contaminados. O objetivo deste estudo foi determinar a prevalência de Salmonellaspp. na carne suína e em ambientes de 6 frigoríficos e 14 açougues selecionados no departamento de Tolima. Foi desenvolvido um por conveniência, a seleção de frigoríficos foi pelo volume de sacrifício e a gestão de boas práticas de manufatura. As amostras foram obtidas (n = 507) de carcaças, ambientes e fômites (ou seja, raspagem de facas, gan- chos, pisos, sifões mesas e caminhões transportadores). Estas amostras foram submetidas à análise microbiológica para o isolamento de Salmonella spp.,a qual foi identificado por testes bioquímicos e serológicos (Poli A + Vi). Os resultados mostraram uma prevalência de 4,3% (25/507) de Salmonella spp. Das 25 amostras positivas 14 (56%) delas foram isoladas de carcaças e os restantes 11 (44%) foram isolados de ambientes, tanto de frigoríficos como dos açougues. Em conclusão, a presença de Salmonella spp. emfases de pós-produção (transformação e comercialização) pode ocorrer por contami- nação por fômites ambientais no transporte e nos pontos de venda e é importante devido ao risco à saúde pública. Este trabalho constitui o primeiro reporte sobre a prevalência de Salmonella spp. em plantas de processamento e açougues, no departamento de Tolima.Palavras chave: plantas de processamento, suínos, produtos carne, Salmonella spp
Modification of (3872) and (2) production in Pb collisions at TeV
The LHCb Collaboration measures production of the exotic hadron χc1(3872) in proton-nucleus collisions for the first time. Comparison with the charmonium state ψ(2S) suggests that the exotic χc1(3872) experiences different dynamics in the nuclear medium than conventional hadrons, and comparison with data from proton-proton collisions indicates that the presence of the nucleus may modify χc1(3872) production rates. This is the first measurement of the nuclear modification factor of an exotic hadron.The LHCb collaboration measures production of the exotic hadron (3872) in proton-nucleus collisions for the first time. Comparison with the charmonium state (2) suggests that the exotic (3872) experiences different dynamics in the nuclear medium than conventional hadrons, and comparison with data from proton-proton collisions indicates that the presence of the nucleus may modify (3872) production rates. This is the first measurement of the nuclear modification factor of an exotic hadron
Helium identification with LHCb
International audienceThe identification of helium nuclei at LHCb is achieved using a method based on measurements of ionisation losses in the silicon sensors and timing measurements in the Outer Tracker drift tubes. The background from photon conversions is reduced using the RICH detectors and an isolation requirement. The method is developed using collision data at recorded by the LHCb experiment in the years 2016 to 2018, corresponding to an integrated luminosity of . A total of around helium and antihelium candidates are identified with negligible background contamination. The helium identification efficiency is estimated to be approximately with a corresponding background rejection rate of up to . These results demonstrate the feasibility of a rich programme of measurements of QCD and astrophysics interest involving light nuclei
Enhanced production of baryons in high-multiplicity collisions at TeV
International audienceThe production rate of baryons relative to mesons in collisions at a center-of-mass energy TeV is measured by the LHCb experiment. The ratio of to production cross-sections shows a significant dependence on both the transverse momentum and the measured charged-particle multiplicity. At low multiplicity, the ratio measured at LHCb is consistent with the value measured in collisions, and increases by a factor of with increasing multiplicity. At relatively low transverse momentum, the ratio of to cross-sections is higher than what is measured in collisions, but converges with the ratio as the momentum increases. These results imply that the evolution of heavy quarks into final-state hadrons is influenced by the density of the hadronic environment produced in the collision. Comparisons with a statistical hadronization model and implications for the mechanisms enforcing quark confinement are discussed
Charge-dependent curvature-bias corrections using a pseudomass method
International audienceMomentum measurements for very high momentum charged particles, such as muons from electroweak vector boson decays, are particularly susceptible to charge-dependent curvature biases that arise from misalignments of tracking detectors. Low momentum charged particles used in alignment procedures have limited sensitivity to coherent displacements of such detectors, and therefore are unable to fully constrain these misalignments to the precision necessary for studies of electroweak physics. Additional approaches are therefore required to understand and correct for these effects. In this paper the curvature biases present at the LHCb detector are studied using the pseudomass method in proton-proton collision data recorded at centre of mass energy TeV during 2016, 2017 and 2018. The biases are determined using decays in intervals defined by the data-taking period, magnet polarity and muon direction. Correcting for these biases, which are typically at the GeV level, improves the mass resolution by roughly 20% and eliminates several pathological trends in the kinematic-dependence of the mean dimuon invariant mass
Enhanced production of baryons in high-multiplicity collisions at TeV
International audienceThe production rate of baryons relative to mesons in collisions at a center-of-mass energy TeV is measured by the LHCb experiment. The ratio of to production cross-sections shows a significant dependence on both the transverse momentum and the measured charged-particle multiplicity. At low multiplicity, the ratio measured at LHCb is consistent with the value measured in collisions, and increases by a factor of with increasing multiplicity. At relatively low transverse momentum, the ratio of to cross-sections is higher than what is measured in collisions, but converges with the ratio as the momentum increases. These results imply that the evolution of heavy quarks into final-state hadrons is influenced by the density of the hadronic environment produced in the collision. Comparisons with a statistical hadronization model and implications for the mechanisms enforcing quark confinement are discussed
Measurement of forward charged hadron flow harmonics in peripheral PbPb collisions at TeV with the LHCb detector
International audienceFlow harmonic coefficients, , which are the key to studying the hydrodynamics of the quark-gluon plasma (QGP) created in heavy-ion collisions, have been measured in various collision systems, kinematic regions, and using various particle species. The study of flow harmonics in a wide pseudorapidity range is particularly valuable to understand the temperature dependence of the shear viscosity to entropy density ratio of the QGP. This paper presents the first LHCb results of the second- and the third-order flow harmonic coefficients of charged hadrons as a function of transverse momentum in the forward region, corresponding to pseudorapidities between 2.0 and 4.9, using the data collected from PbPb collisions in 2018 at a center-of-mass energy of TeV. The coefficients measured using the two-particle angular correlation analysis method are smaller than the central-pseudorapidity measurements at ALICE and ATLAS from the same collision system but share similar features
Observation of and decays
International audienceThe decays and are observed for the first time, in proton-proton collision data at TeV corresponding to an integrated luminosity of 5.4 fb collected with the LHCb detector. Their ratios of branching fractions with respect to the mode are measured to be \begin{align*} \begin{split} \frac{\mathcal{B}(\Lambda_b^0 \to \Lambda_c^+\bar{D}^0 K^-)}{\mathcal{B}(\Lambda_b^0 \to \Lambda_c^+ D_s^-)} & = 0.1908 {}_{-0.0034}^{+0.0036} {}_{-0.0018}^{+0.0016} \pm 0.0038 \\ \frac{\mathcal{B}(\Lambda_b^0 \to \Lambda_c^+\bar{D}^{*0} K^-)}{\mathcal{B}(\Lambda_b^0 \to \Lambda_c^+ D_s^-)} & = 0.589 {}_{-0.017}^{+0.018} {}_{-0.018}^{+0.017} \pm 0.012 \\ \frac{\mathcal{B}(\Lambda_b^0 \to \Lambda_c^+ D_s^{*-})}{\mathcal{B}(\Lambda_b^0 \to \Lambda_c^+ D_s^-)} & = 1.668 \pm 0.022 {}_{-0.055}^{+0.061}\ , \end{split} \end{align*} where the first uncertainties are statistical, the second systematic, and the third, for the decays, are due to the uncertainties on the branching fractions of the and decay modes. The measured branching fractions probe factorization assumptions in effective theories and provide the normalization for future pentaquark searches in decay channels
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