The role of ras gene in the development of haemic neoplasia in Mytilus trossulus

Disseminated neoplasia has been reported in mussels (Mytilus spp) from numerous locations worldwide. This condition is progressive and fatal and the aetiology is unknown. In vertebrates, oncogenes such as ras, and tumour suppressor genes such as p53, play important roles in carcinogenesis. We have cloned a Mytilus trossulus homologue of the vertebrate ras gene, which shows conserved sequence in regions of functional importance. Neoplastic hemolymph samples derived from M. trossulus have been investigated for the presence of ras gene mutations and changes in expression

Light Lepton Number Violating Sneutrinos and the Baryon Number of the Universe

Recent results of neutrino oscillation experiments point to a nonvanishing neutrino mass. Neutrino mass models favour Majorana-type neutrinos. In such circumstances it is natural that the supersymmetric counterpart of the neutrino, the sneutrino, bears also lepton number violating properties. On the other hand, the fact that the universe exhibits an asymmetry in the baryon and antibaryon numbers poses constraints on the extent of lepton number violation in the light sneutrino sector if the electroweak phase transition is second or weak first order. From the requirement that the Baryon Asymmetry of the Universe should not be washed out by sneutrino induced lepton number violating interactions and sphalerons below the critical temperature of the electroweak phase transition we find that the mass splitting of the light sneutrino mass states is compatible with the sneutrino Cold Dark Matter hypothesis only for heavy gauginos and opposite sign gaugino mass parameters.Comment: 13 pages, 4 figure

Sedimentation and subsidence history of the Lomonosov Ridge

During the first scientific ocean drilling expedition to the Arctic Ocean (Arctic Coring Expedition [ACEX]; Integrated Ocean Drilling Program Expedition 302), four sites were drilled and cored atop the central part of the Lomonosov Ridge in the Arctic Ocean at ~88°N, 140°E (see Fig. F18 in the "Sites M0001–M0004" chapter). The ridge was rifted from the Eurasian continental margin at ~57 Ma (Fig. F1) (Jokat et al., 1992, 1995). Since the rifting event and the concurrent tilting and erosion of this sliver of the outer continental margin, the Lomonosov Ridge subsided while hemipelagic and pelagic sediments were deposited above the angular rifting unconformity (see Fig. F7A in the "Sites M0001–M0004" chapter).The sections recovered from the four sites drilled during Expedition 302 can be correlated using their seismic signature, physical properties (porosity, magnetic susceptibility, resistivity, and P-wave velocity), chemostratigraphy (ammonia content of pore waters), lithostratigraphy, and biostratigraphy. The lithostratigraphy of the composite section combined with biostratigraphy provides an insight into the complex history of deposition, erosion, and preservation of the biogenic fraction. Eventually, the ridge subsided to its present water depth as it drifted from the Eurasian margin. In this chapter, we compare a simple model of subsidence history with the sedimentary record recovered from atop the ridge

Accuracy of diabetes screening methods used for people with tuberculosis, Indonesia, Peru, Romania, South Africa

Objective To evaluate the performance of diagnostic tools for diabetes mellitus, including laboratory methods and clinical risk scores, in newly-diagnosed pulmonary tuberculosis patients from four middle-income countries. Methods In a multicentre, prospective study, we recruited 2185 patients with pulmonary tuberculosis from sites in Indonesia, Peru, Romania and South Africa from January 2014 to September 2016. Using laboratory-measured glycated haemoglobin (HbA1c) as the gold standard, we measured the diagnostic accuracy of random plasma glucose, point-of-care HbA1c, fasting blood glucose, urine dipstick, published and newly derived diabetes mellitus risk scores and anthropometric measurements. We also analysed combinations of tests, including a two-step test using point-of-care HbA1cwhen initial random plasma glucose was ≥ 6.1 mmol/L. Findings The overall crude prevalence of diabetes mellitus among newly diagnosed tuberculosis patients was 283/2185 (13.0%; 95% confidence interval, CI: 11.6–14.4). The marker with the best diagnostic accuracy was point-of-care HbA1c (area under receiver operating characteristic curve: 0.81; 95% CI: 0.75–0.86). A risk score derived using age, point-of-care HbA1c and random plasma glucose had the best overall diagnostic accuracy (area under curve: 0.85; 95% CI: 0.81–0.90). There was substantial heterogeneity between sites for all markers, but the two-step combination test performed well in Indonesia and Peru. Conclusion Random plasma glucose followed by point-of-care HbA1c testing can accurately diagnose diabetes in tuberculosis patients, particularly those with substantial hyperglycaemia, while reducing the need for more expensive point-of-care HbA1c testing. Risk scores with or without biochemical data may be useful but require validation

Appetite suppressants and valvular heart disease - a systematic review

Background Although appetite suppressants have been implicated in the development of valvular heart disease, the exact level of risk is still uncertain. Initial studies suggested that as many as 1 in 3 exposed patients were affected, but subsequent research has yielded substantially different figures. Our objective was to systematically assess the risk of valvular heart disease with appetite suppressants. Methods We accepted studies involving obese patients treated with any of the following appetite suppressants: fenfluramine, dexfenfluramine, and phentermine. Three types of studies were reviewed: controlled and uncontrolled observational studies, and randomized controlled trials. Outcomes of interest were echocardiographically detectable aortic regurgitation of mild or greater severity, or mitral regurgitation of moderate or greater severity. Results Of the 1279 patients evaluated in seven uncontrolled cohort studies, 236 (18%) and 60 (5%) were found to have aortic and mitral regurgitation, respectively. Pooled data from six controlled cohort studies yielded, for aortic regurgitation, a relative risk ratio of 2.32 (95% confidence intervals 1.79 to 3.01, p < 0.00001) and an attributable rate of 4.9%, and for mitral regurgitation, a relative risk ratio of 1.55 (95% confidence intervals 1.06 to 2.25, p = 0.02) with an attributable rate of 1.0%. Only one case of valvular heart disease was detected in 57 randomized controlled trials, but this was judged unrelated to drug therapy. Conclusions The risk of valvular heart disease is significantly increased by the appetite suppressants reviewed here. Nevertheless, when considering all the evidence, valvulopathy is much less common than suggested by the initial, less methodologically rigorous studies

Expedition 302 geophysics: integrating past data with new results

In preparation for IODP Expedition 302, Arctic Coring Expedition (ACEX), a site survey database comprising geophysical and geological data from the Lomonosov Ridge was compiled. The accumulated database includes data collected from ice islands, icebreakers, and submarines from 1961 to 2001. In addition, seismic reflection profiles were collected during Expedition 302 that complement the existing seismic reflection data and facilitate integration between the acoustic stratigraphy and the Expedition 302 drill cores. An overview of these data is presented in this chapter.It is well recognized that collecting geophysical data in ice-covered seas, in particular the Arctic Ocean, is a challenging endeavor. This is because much of the Arctic Ocean is continuously covered with ice thicknesses that vary from 1 to 6 m. Over the continental shelves, sea ice can be absent during summer months, but it is present year-round in the central basins. This ice cover is the most dominant feature of the Arctic Ocean environment. It circulates in the ocean basin in two main circulation patterns: the Transpolar Drift and the Beaufort Gyre (see the "Expedition 302 summary" chapter; Rudels et al., 1996).Expedition 302 sites are located within the less severe of these two ice circulation systems, the Transpolar Drift, which primarily moves sea ice from the shelves where it is formed (the Laptev and East Siberian Seas) across the basin and exits through the Fram Strait. During late summer, concentrations of Arctic sea ice can be &lt;100% (10/10 ice cover), making it possible for icebreakers to operate. Average ice concentrations in the central Arctic Ocean during summer months can locally vary from partially open water (6/10) to completely ice covered (10/10). This sea-ice cover can move at speeds up to 0.5 kt.Early Arctic Ocean geophysical exploration was performed from ice-drift stations (Weber and Roots, 1990). However, the tracks from these drifting ice stations were controlled "by the whims of nature" (Jackson et al., 1990), preventing detailed, systematic surveys of predetermined target areas. These ice-drift stations were set up on stable icebergs that were trapped in sea ice and moved generally with the large drift patterns, but locally they were erratic, so preselected locations could not be surveyed. In the late 1980s, single icebreakers began to be used for oceanographic survey work in the Arctic Ocean. Between 1991 and 2001, four scientific icebreaker expeditions to the Lomonosov Ridge took place. These cruises all experienced local sea-ice conditions varying between 8/10 and 10/10. During these expeditions, towed geophysical equipment was occasionally damaged or lost, either because of a rapidly closing wake caused by local ice pressure or because ice had cut the air gun array.Conventionally powered icebreakers reached as far as the North Pole for the first time during the 1991 Expedition (Andersen and Carlsonn, 1992; Fütterer, 1992). Geophysical results from this expedition collected two important reflection profiles, AWI-91090 and AWI-91091, that crossed the Lomonosov Ridge between 87° and 88°N. These profiles imaged a ~450 m thick, well-stratified and apparently undisturbed drape of sediments overlying a prominent acoustic unconformity (Jokat et al., 1992) that spawned the idea to conduct a paleoceanographic drilling expedition to this Ridge.The use of US Navy nuclear submarines for geophysical mapping was implemented through the Science Ice Exercise program (SCICEX) (Newton, 2000). The development of the Seafloor Characterization and Mapping Pods (SCAMP), which hold a Chirp subbottom profiler, swath bathymetric profiler, and side scan sonar, was an essential part of the SCICEX program (Chayes et al., 1996). In 1999, the Lomonosov Ridge geophysical database was augmented with acoustic data acquired during the SCICEX program using the SCAMP system mounted on the US nuclear submarine USS Hawkbill (Edwards and Coakley, 2003)

The UK risk assessment scheme for all non-native species

1. A pest risk assessment scheme, adapted from the EPPO (European and Mediterranean Plant Protection Organisation) scheme, was developed to assess the risks posed to UK species, habitats and ecosystems by non-native taxa. 2. The scheme provides a structured framework for evaluating the potential for non-native organisms, whether intentional or unintentional introductions, to enter, establish, spread and cause significant impacts in all or part of the UK. Specialist modules permit the relative importance of entry pathways, the vulnerability of receptors and the consequences of policies to be assessed and appropriate risk management options to be selected. Spreadsheets for summarising the level of risk and uncertainty, invasive attributes and economic impact were created. In addition, new methods for quantifying economic impact and summarising risk and uncertainty were explored. 3. Although designed for the UK, the scheme can readily be applied elsewhere

Expedition 302 summary

The first scientific drilling expedition to the central Arctic Ocean was completed in September 2004. Integrated Ocean Drilling Program Expedition 302, Arctic Coring Expedition (ACEX), recovered sediment cores to 428 meters below seafloor (mbsf) in water depths of ~1300 m, 250 km from the North Pole.Expedition 302's destination was the Lomonosov Ridge, hypothesized to be a sliver of continental crust that broke away from the Eurasian plate at ~56 Ma. As the ridge moved northward and subsided, marine sedimentation occurred and continues to the present, resulting in what was anticipated from seismic data to be a continuous paleoceanographic record. The elevation of the ridge above the surrounding abyssal plains (~3 km) ensured that sediments atop the ridge were free of turbidites. The primary scientific objective of Expedition 302 was to continuously recover this sediment record and to sample the underlying sedimentary bedrock by drilling and coring from a stationary drillship.The biggest challenge during Expedition 302 was maintaining the drillship's location while drilling and coring in 2–4 m thick sea ice that moved at speeds approaching 0.5 kt. Sea-ice cover over the Lomonosov Ridge moves with one of the two major Arctic sea-ice circulation systems, the Transpolar Drift, and responds locally to wind, tides, and currents. Until now, the high Arctic Ocean Basin, known as "mare incognitum" within the scientific community, had never before been deeply cored because of these challenging sea-ice conditions.Initial results reveal that biogenic carbonate is present only in the Holocene–Pleistocene interval. The upper 198 mbsf represents a relatively high sedimentation rate record of the past 18 m.y. and is composed of sediment with ice-rafted debris and dropstones, suggesting that ice-covered conditions extended at least this far back in time. Details of the ice type (e.g., iceberg versus sea ice), timing, and characteristics (e.g., perennial versus seasonal) await further study. A hiatus occurs at 193.13 mbsf, spanning a 25 m.y. interval from the early Miocene to the middle Eocene between ~18 Ma and 43 Ma. The sediment record during the middle Eocene is of dark, organic-rich biosiliceous composition. Isolated pebbles, interpreted as ice-rafted dropstones, are present down to 239 mbsf, well into this middle Eocene interval. Around the lower/middle Eocene boundary an abundance of Azolla spp. occurs, suggesting that a fresh and/or low-salinity surface water setting dominated the region during this time period. Although predrilling predictions based on geophysical data had placed the base of the sediment column at 50 Ma, drilling revealed that the uppermost Paleocene to lowermost Eocene boundary interval, well known as the Paleocene/Eocene Thermal Maximum (PETM), was recovered. During the PETM, the temperature of the Arctic Ocean surface waters exceeded 20°C.Drilling during Expedition 302 also penetrated into the underlying sedimentary bedrock, revealing a shallow-water depositional environment of Late Cretaceous age