Glycosidase-catalyzed synthesis of glycosylated nutraceutical ingredients

Hydroxyphenyl propenoic acids (hydroxycinnamic acids) and their alcohol derivatives are common components of the human diet which often occur in plants in the form of various glycosides. As the diets rich in polyphenols have repeatedly been related to low incidence of cardiovascular, neurodegenerative, and oncological diseases, various food supplements containing these compounds are becoming increasingly popular among the general population. In quest of a biocatalytic route to structurally complex phenolic glycosides, we built a sustainable and convenient, one-pot two-enzyme method for the glucosylation of arylalkyl alcohols based on the synthetic exploitation of a fungal rutinosidase from A. niger and rhamnosidase from A. terreus. Both these enzymes were available to us as heterologous proteins produced by a recombinant strain of P. pastoris. As an example, the -glucoside salidroside, a compound endowed with various pharmacological effects and commercialized in Rhodiola rosea nutraceutical formulations, was obtained in high isolated yield and purity from tyrosol thanks to our one-pot enzymatic process. Furthermore, during the course of our investigation, we found that the rutinosidase from A. niger not only efficiently converted hydroxylated aromatic acids (e.g. coumaric and ferulic acids) into the respective phenolic rutinosides, but surprisingly could also catalyze the formation of the respective glycosyl esters. Here the results of our systematic study about the glycosidase-based biocatalytic preparation of lycosylated nutraceutical ingredients, which lead us to the discovery of a unique enzymatic entry to naturally occurring glycosyl esters, are reported

Results on entire solutions for a degenerate critical elliptic equation with anisotropic coefficients

In this paper, we study the following degenerate critical elliptic equations with anisotropic coefficients $$-div(|x_{N}|^{2\alpha}\nabla u)=K(x)|x_{N}|^{\alpha\cdot 2^{*}(s)-s}|u|^{2^{*}(s)-2}u {in} \mathbb{R}^{N}$$ where $x=(x_{1},...,x_{N})\in\mathbb{R}^{N},$ $N\geq 3,$ $\alpha>1/2,$ $0\leq s\leq 2$ and $2^{*}(s)=2(N-s)/(N-2).$ Some basic properties of the degenerate elliptic operator $-div(|x_{N}|^{2\alpha}\nabla u)$ are investigated and some regularity, symmetry and uniqueness results for entire solutions of this equation are obtained. We also get some variational identities for solutions of this equation. As a consequence, we obtain some nonexistence results for solutions of this equation.Comment: 29 page

First prototype of a silicon tracker using an artificial retina for fast track finding

We report on the R\&D for a first prototype of a silicon tracker based on an alternative approach for fast track finding. The working principle is inspired from neurobiology, in particular by the processing of visual images by the brain as it happens in nature. It is based on extensive parallelisation of data distribution and pattern recognition. In this work we present the design of a practical device that consists of a telescope based on single-sided silicon detectors; we describe the data acquisition system and the implementation of the track finding algorithms using available digital logic of commercial FPGA devices. Tracking performance and trigger capabilities of the device are discussed along with perspectives for future applications.Comment: 9 pages, 7 figures, Technology and Instrumentation in Particle Physics 2014 (TIPP 2014), conference proceeding

Effect of polyvinyl alcohol ligands on supported gold nano-catalysts: Morphological and kinetics studies

The effect of polyvinyl alcohol (PVA) stabilizers and gold nanoparticles supported on active carbon (AuNPs/AC) was investigated in this article. Polymers with different molecular weights and hydrolysis degrees have been synthesized and used, like the stabilizing agent of Au nano-catalysts obtained by the sol-immobilization method. The reduction of 4-nitrophenol with NaBH4 has been used as a model reaction to investigate the catalytic activity of synthesized Au/AC catalysts. In addition, we report several characterization techniques such as ultraviolet-visible spectroscopy (UV-Vis), dynamic light scattering (DLS), X-ray diffraction (XRD), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS) in order to correlate the properties of the polymer with the metal nanoparticle size and the catalytic activity. A volcano plot was observed linking the catalytic performance with hydrolysis degree and the maximum of the curve was identified at a value of 60%. The Au:PVA-60 weight ratio was changed in order to explain how the amount of the polymer can influence catalytic properties. The effect of nitroaromatic ring substituents on the catalytic mechanism was examined by the Hammett theory. Moreover, the reusability of the catalyst was investigated, with little to no decrease in activity observed over five catalytic cycles. Morphological and kinetic studies reported in this paper reveal the effect of the PVA polymeric stabilizer properties on the size and catalytic activity of supported gold nanoparticles

Total Cost of Ownership of Digital vs. Analog Radio-Over-Fiber Architectures for 5G Fronthauling

The article analyzes the total cost of ownership (TCO) of 5G fronthauling solutions based on analog and digital radio-over-fiber (RoF) architectures in cloud radio access networks (C-RANs). The capital and operational expenditures (CAPEX, OPEX) are assessed, for a 10-year period, considering three different RoF techniques: intermediate frequency analog RoF (IF-A-RoF), digital signal processing (DSP) assisted analog RoF (DSP-A-RoF), and digital RoF (D-RoF) based on the common public radio interface (CPRI) specifications. The greenfield deployment scenario under exam includes both fiber trenching (FT) and fiber leasing (FL) options. The TCO is assessed while varying (i) the number of aggregated subcarriers, (ii) the number of three-sector antennas located at the base station, and (iii) the mean fiber-hop length. The comparison highlights the significance that subcarrier aggregation has on the cost efficiency of the analog RoF solutions. In addition, the analysis details the contribution of each cost category to the overall CAPEX and OPEX values. The obtained results indicate that subcarrier aggregation via DSP results in high cost efficiency for a mobile fronthaul network, while a CPRI-based architecture together with FL brings the highest OPEX value

Microwave pyrolysis of biomass for bio-oil production: Scalable processing concepts

The pursuit of sustainable hydrocarbon alternatives to fossil fuels has prompted an acceleration in the development of new technologies for biomass processing. Microwave pyrolysis of biomass has long been recognised to provide better quality bio-products in shorter timescales compared to conventional pyrolysis. Although this topic has been widely assessed and many investigations are currently ongoing, this article gives an overview beyond the physico-chemical pyrolysis process and covers engineering aspects and the limitations of microwave heating technology. Herein, we provide innovative scalable concepts to perform the microwave pyrolysis of biomass on a large scale, including essential energy and material handling requirements. Furthermore, some of the possible socio-economic and environmental implications derived from the use of this technology in our society are discussed. Such potential concepts are expected to assist the needs of the industrial bioenergy community to move this largely studied process upwards in scale

Mechanical and thermal properties of crab chitin reinforced carboxylated SBR composites

The addition of small amounts (up to 9 wt%) of chitin microsized particles, originating from shellfish waste, to carboxylated styrene-butadiene rubber (XSBR) matrix (as received and annealed to 100°C) has been studied. In particular, this study concentrated on their mechanical (creep investigation by nanoindentation and dynamical-mechanical analysis), thermal (differential scanning calorimetry and thermogravimetry) and swelling behaviour (toluene absorption) and was completed by morphological characterisation by scanning electron microscopy and atomic force microscopy. The results show that annealing has a limited effect on materials properties, effects which are further reduced by the addition of growing amounts of crab chitin. It should be noted that the limited filler content used in the study does not substantially modify the linear creep behaviour of XSBR for sufficiently long loading times. The thermal stability of the system does also appear to be preserved even with the maximum chitin content added, while it serves sufficiently as an effective barrier against aromatic solvent absorption

Unsupervised Clustering of Quantitative Imaging Phenotypes using Autoencoder and Gaussian Mixture Model

Quantitative medical image computing (radiomics) has been widely applied to build prediction models from medical images. However, overfitting is a significant issue in conventional radiomics, where a large number of radiomic features are directly used to train and test models that predict genotypes or clinical outcomes. In order to tackle this problem, we propose an unsupervised learning pipeline composed of an autoencoder for representation learning of radiomic features and a Gaussian mixture model based on minimum message length criterion for clustering. By incorporating probabilistic modeling, disease heterogeneity has been taken into account. The performance of the proposed pipeline was evaluated on an institutional MRI cohort of 108 patients with colorectal cancer liver metastases. Our approach is capable of automatically selecting the optimal number of clusters and assigns patients into clusters (imaging subtypes) with significantly different survival rates. Our method outperforms other unsupervised clustering methods that have been used for radiomics analysis and has comparable performance to a state-of-the-art imaging biomarker.Comment: Accepted at MICCAI 201