Monotonicity of a profile of rankings with ties

International Conference, IPMU (17th. 2018. Cádiz

Escuela para hipertensos y modificación de estilos de vida

ntroducción: La hipertensión arterial está distribuida en todas las regiones del mundo, atendiendo a múltiples factores de índole económicos, sociales, culturales, ambientales y étnicos. La prevalencia ha estado en aumento, asociada a patrones alimentarios inadecuados, disminución de la actividad física y  hábitos tóxicos. Objetivo: Modificar  aspectos del estilo de vida  y  así lograr el control de la  hipertensión arterial. Método: Se realizó un estudio de intervención educativa en 210 pacientes hipertensos pertenecientes a los grupos básicos de trabajo 1 y 2  del  Policlínico Universitario “José Martí Pérez” de Santiago de Cuba, en el período comprendido desde  marzo del 2013 a marzo del  2014, interrelacionándose variables clínicas y sociodemográficas de interés, a los resultados se les aplicó como medida de resumen el porcentaje. Resultados: Encontramos que la enfermedad fue más frecuente en el sexo femenino entre la 5ta y 6ta década de la vida, predominio de hábitos tóxicos, con mala comunicación, escasa disponibilidad del tiempo libre y poca práctica de ejercicios físicos, así como conducta alimentaria inadecuada. En estos pacientes predominaron las cifras tensionales por encima de 140/90 mmHg. Conclusiones: Con la intervención realizada se lograron modificaciones positivas en el estilo de vida de estos pacientes en relación a los aspectos antes señalados, así como mayor control de las cifras tensionales y un aumento del nivel de conocimientos sobre la enfermedad. Palabras claves: hipertensión arterial, factores de riesgo, atención primaria de salud

Chronic environmentally relevant levels of simvastatin disrupt embryonic development, biochemical and molecular responses in zebrafish (Danio rerio)

This is the postprint (accepted manuscript) version of the article published in Aquatic Toxicology. https://doi.org/10.1016/j.aquatox.2018.05.014Simvastatin (SIM), a hypocholesterolaemic compound, is among the most prescribed pharmaceuticals for cardiovascular disease prevention worldwide. Several studies have shown that acute exposure to SIM causes multiple adverse effects in aquatic organisms. However, uncertainties still remain regarding the chronic effects of SIM in aquatic ecosystems. Therefore, the present study aimed to investigate the effects of SIM in the model freshwater teleost zebrafish (Danio rerio) following a chronic exposure (90 days) to environmentally relevant concentrations ranging from 8 ng/L to 1000 ng/L. This study used a multi-parameter approach integrating distinct ecologically-relevant endpoints, i.e. survival, growth, reproduction and embryonic development, with biochemical markers (cholesterol and triglycerides). Real Time PCR was used to analyse the transcription levels of key genes involved in the mevalonate pathway (hmgcra, cyp51, and dhcr7). Globally, SIM induced several effects that did not follow a dose-response relationship; embryonic development, biochemical and molecular markers, were significantly impacted in the lower concentrations, 8 ng/L, 40 ng/L and/or 200 ng/L, whereas no effects were recorded for the highest tested SIM levels (1000 ng/L). Taken together, these findings expand our understanding of statin effects in teleosts, demonstrating significant impacts at environmentally relevant concentrations and highlight the importance of addressing the effects of chemicals under chronic low-level concentrationsT. Neuparth was supported by the Postdoctoral fellowship SFRH/BPD/77912/2011 from Foundation of Science and Technology, Portugal. R. Montes, R. Rodil and J.B. Quintana acknowledge financial support from the Spanish “Agencia Estatal de Investigación” (project no. CTM2014-56628-C3-2-R), the Galician Council of Culture, Education and Universities (ED431C2017/36) and FEDER/ERDFS

Transcriptomic data on the transgenerational exposure of the keystone amphipod Gammarus locusta to simvastatin

The use of transcriptomics data brings new insights and works as a powerful tool to explore the molecular mode of action (MoA) of transgenerational inheritance effects of contaminants of emerging concern. Therefore, in this dataset, we present the transcriptomic data of the transgenerational effects of environmentally relevant simvastatin levels, one of the most prescribed human pharmaceuticals, in the keystone amphipod species Gammarus locusta. In summary, G. locusta juveniles were maintained under simvastatin exposure up to adulthood (exposed group - F0E) and the offspring of F0E were transferred to control water for the three subsequent generations (transgenerational group - F1T, F2T and F3T). To gain insights into the biological functions and canonical pathways transgenerationally disrupted by simvastatin, a G. locusta de novo transcriptome assembly was produced and the transcriptomic profiles of three individual G. locusta females, per group, over the four generations (F0 to F3) - solvent control groups (F0.C, F1.C, F2.C and F3.C), F0 320 ng/L simvastatin exposed group (F0.320E) and F1 to F3 320 transgenerational group (F1.320T; F2.320T and F3.320T) - were analyzed. Briefly, Illumina HiSeq™ 2500 platform was used to perform RNA sequencing, and due to the unavailability of G. locusta genome, the RNA-seq datasets were assembled de novo using Trinity and annotated with Trinotate software. After assembly and post-processing steps, 106093 transcripts with N50 of 2371 bp and mean sequence length of 1343.98 bp was produced. BUSCO analyses showed a transcriptome with gene completeness of 97.5 % Arthropoda library profile. The Bowtie2, RSEM and edgeR tools were used for the differential gene expression (DEGs) analyses that allowed the identification of a high quantity of genes differentially expressed in all generations. Finally, to identify the main metabolic pathways affected by the transgenerational effects of SIM across all generations, the DGEs genes were blasted onto KEGG pathways database using the KAAS webserver. The data furnished in this article allows a better molecular understanding of the transgenerational effects produced by simvastatin in the keystone amphipod G. locusta and has major implications for hazard and risk assessment of pharmaceuticals and other emerging contaminants. This article is related to the research article entitled “Transgenerational inheritance of chemical-induced signature: a case study with simvastatinThis article was developed under the Transobesogen project - Trans-phyletic obesogenic responses: from epigenetic modules to transgenerational environmental impacts (reference PTDC/CTA-AMB/31544/2017 - NORTE-01-0145-FEDER-031544), cofunded by Portugal 2020, the European Union through the ERDF and by the Portuguese Foundation for Science and Technology – FCT. This article was also supported by FCT through national funds (UIDB/04423/2020; UIDP/04423/2020), by the Spanish Agencia Estatal de Investigación (CTM2017-84763-C3-2-R) and by the Galician Council of Culture, Education and Universities (ED431C2017/36), cofounded by ERDF. A PhD grant awarded to Susana Barros acknowledges the doctoral grant attributed by FCT with reference PD/BD/143090/2018S