Dynamic self-referencing approach to whispering gallery mode biosensing and its application to measurement within undiluted serum

Biosensing within complex biological samples requires a sensor that can compensate for fluctuations in the signal due to changing environmental conditions and nonspecific binding events. To achieve this, we developed a novel self-referenced biosensor consisting of two almost identically sized dye-doped polystyrene microspheres placed on adjacent holes at the tip of a microstructured optical fiber (MOF). Here self-referenced biosensing is demonstrated with the detection of Neutravidin in undiluted, immunoglobulin-deprived human serum samples. The MOF allows remote excitation and collection of the whispering gallery modes (WGMs) of the microspheres while also providing a robust and easy to manipulate dip-sensing platform. By taking advantage of surface functionalization techniques, one microsphere acts as a dynamic reference, compensating for nonspecific binding events and changes in the environment (such as refractive index and temperature), while the other microsphere is functionalized to detect a specific interaction. The almost identical size allows the two spheres to have virtually identical refractive index sensitivity and surface area, while still having discernible WGM spectra. This ensures their responses to nonspecific binding and environmental changes are almost identical, whereby any specific changes, such as binding events, can be monitored via the relative movement between the two sets of WGM peaks.Tess Reynolds, Alexandre Franc, ois, Nicolas Riesen, Michelle E. Turvey, Stephen J. Nicholls, Peter Hoffmann, and Tanya M. Monr

Driving down the Detection Limit in Microstructured Fiber-Based Chemical Dip Sensors

We present improvements to fluorescence sensing in soft-glass microstructured optical fibers that result in significantly improved sensitivity relative to previously published results. Concentrations of CdSe quantum dots down to 10 pM levels have been demonstrated. We show that the primary limitation to the sensitivity of these systems is the intrinsic fluorescence of the glass itself

Cancer detection in human tissue samples using a fiber-tip pH probe

Abstract not availableErik P. Schartner, Matthew R. Henderson, Malcolm Purdey, Deepak Dhatrak, Tanya M. Monro, P. Grantley Gill, and David F. Calle

Advances and limitations in the modelling of fabricated photonic bandgap fibers

Copyright © 2006 IEEEWe model fabricated silica photonic bandgap fibers and achieve good agreement between simulated and measured properties. We identify the size of the SEM bitmap image as the ultimate limit to the accurate calculation of surfaces modes within the bandgapF. Poletti, M. N. Petrovich, R. Amezcua-Correa, N. G. Broderick, T. M. Monro and D. J. Richardsonhttp://eprints.soton.ac.uk/47883

Q-factor limits for far-field detection of whispering gallery modes in active microspheres

Abstract not availableNicolas Riesen,Tess Reynolds, Alexandre François, Matthew R. Henderson, and Tanya M. Monr

Optimization of whispering gallery mode sensor design for applications in biosensing

Abstract not availableT. Reynolds, A. François, M.R. Henderson, S.J. Nicholls, T.M Monr

Nonlinear polarization bistability in optical nanowires

Using the full vectorial nonlinear Schrödinger equations that describe nonlinear processes in isotropic optical nanowires, we show that there exist structural anisotropic nonlinearities that lead to unstable polarization states that exhibit periodic bistable behavior. We analyze and solve the nonlinear equations for continuous waves by means of a Lagrangian formulation and show that the system has bistable states and also kink solitons that are limiting forms of the bistable states.Wen Qi Zhang, M. A. Lohe, Tanya M. Monro, and Shahraam Afshar V

Enhancing the radiation efficiency of dye doped whispering gallery mode microresonators

We present a novel form of a Whispering Gallery Mode (WGM) sensor that exploits dye doped polystyrene microspheres, as active resonators, positioned onto the tip of a Microstructured Optical Fiber (MOF) as a means of overcoming the limited Q-factors for small resonators. We show that it is possible to substantially enhance the fluorescence emission of selected WGMs of the microspheres, resulting in an increase of the signal-to-noise ratio of the modes and of the effective Q-factor. This is done by positioning the resonator into one of the holes of a suspended core MOF and matching the resonator diameter with the hole diameter where it sits, effectively breaking the symmetry of the environment surrounding the sphere. Furthermore we demonstrate that using this experimental configuration, the lasing efficiency of the dye-doped microspheres is also significantly enhanced, which also contributes to an enhancement in the observed Q-factor.Alexandre François, Kristopher J. Rowland, Shahraam Afshar V., Matthew R. Henderson, and Tanya M. Monr

Quantification of the fluorescence sensing performance of microstructured optical fibers compared to multi-mode fiber tips

Published 3 Aug 2016Microstructured optical fibers, particularly those with a suspended-core geometry, have frequently been argued as efficient evanescent-field fluorescence-based sensors. However, to date there has not been a systematic comparison between such fibers and the more common geometry of a multi-mode fiber tip sensor. In this paper we make a direct comparison between these two fiber sensor geometries both theoretically and experimentally. Our results confirm that suspended-core fibers provide a significant advantage in terms of total collected fluorescence signal compared to multi-mode fibers using an equivalent experimental configuration.Erik P. Schartner, Georgios Tsiminis, Matthew R. Henderson, Stephen C. Warren-Smith, and Tanya M. Monr

Localised hydrogen peroxide sensing for reproductive health

Session 10 - Chemical Sensors and Biosensors IIThe production of reactive oxygen species (ROS) is known to affect the developmental competence of embryos. Hydrogen peroxide (H₂O₂) an important reactive oxygen species, is also known to causes DNA damage and defective sperm function. Current techniques require incubating a developing embryo with an organic fluorophore which is potentially hazardous for the embryo. What we need is a localised ROS sensor which does not require fluorophores in solution and hence will allow continuous monitoring of H₂O₂ production without adversely affect the development of the embryo. Here we report studies on such a fibre-based sensor for the detection of H₂O₂ that uses a surface-bound aryl boronate fluorophore carboxyperoxyfluor-1(CPF1). Optical fibres present a unique platform due to desirable characteristics as dip sensors in biological solutions. Attempts to functionalise the fibre tips using polyelectrolyte layers and (3-aminopropyl)triethoxysilane (APTES) coatings resulted in a limited signal and poor fluorescent response to H₂O₂ due to a low tip surface density of the fluorophore. To increase the surface density, CPF1 was integrated into a polymer matrix formed on the fibre tip by a UV-catalysed polymerisation process of acrylamide onto a methacrylate silane layer. The polyacrylamide containing CPF1 gave a much higher surface density than previous surface attachment methods and the sensor was found to effectively detect H₂O₂. Using this method, biologically relevant concentrations of H₂O₂ were detected, enabling remote sensing studies into ROS releases from embryos throughout early development.Malcolm S Purdey, Erik P Schartner, Melanie L Sutton-McDowall, Lesley J Ritter, Jeremy G Thompson, Tanya M Monro, and Andrew D Abel