Local adaptation to hosts and parasitoids shape Hamiltonella defensa genotypes across aphid species

Facultative symbionts are common in insects and can provide their hosts with significant adaptations. Yet we still have a limited understanding of what shapes their distributions, such as why particular symbiont strains are common in some host species yet absent in others. To address this question, we genotyped the defensive symbiont Hamiltonella defensa in 26 aphid species that commonly carry this microbe. We found that Hamiltonella strains were strongly associated with specific aphid species and that strains found in one host species rarely occurred in others. To explain these associations, we reciprocally transferred the Hamiltonella strains of three aphid species, Acyrthosiphon pisum, Macrosiphoniella artemisiae and Macrosiphum euphorbiae, and assessed the impact of Hamiltonella strain on: the stability of the symbiosis, aphid fecundity and parasitoid resistance. We demonstrate that the Hamiltonella strains found in nature are locally adapted to specific aphid hosts, and their ecology: aphids tend to carry Hamiltonella strains that are efficiently transmitted to their offspring, non-lethal, and that provide strong protection against their dominant parasitoid species. Our results suggest that facultative symbiont distributions are shaped by selection from natural enemies, and the host itself, resulting in locally adapted symbioses that provide significant benefits against prevailing natural enemies

A strategy to qualify the performance of radiographic monitors

The purpose of this work was to compare standard desktop display systems with dedicated medical display systems. The set of image tests proposed by the American Association of Physicists in Medicine (AAPM TG18) was used to assess a Philips 107S desktop display system and a Siemens medical display. Three observers performed the subjective assessment, in a non-concerted manner. The objective assessment was performed using a CCD camera according to the AAPM TG18 procedure. The results clearly demonstrate the inadequacy of standard desktop display systems in the framework of diagnostic radiology. Moreover, a good correlation between the subjective and objective assessment methods was obtaine

Parallel Evolution in the Integration of a Co-obligate Aphid Symbiosis

Dependence on multiple nutrient- provisioning symbionts has evolved numerous times in insects. Monnin et al. provide evidence from the symbionts of aphids that these dependencies evolve in a predictable manner. The repeated losses of the same metabolic pathways bind the symbionts into co-dependence, and integration follows in a stepwise manner

Management of patient dose and image noise in routine pediatric CT abdominal examinations

The aim was to propose a strategy for finding reasonable compromises between image noise and dose as a function of patient weight. Weighted CT dose index (CTDIw) was measured on a multidetector-row CT unit using CTDI test objects of 16, 24 and 32cm in diameter at 80, 100, 120 and 140kV. These test objects were then scanned in helical mode using a wide range of tube currents and voltages with a reconstructed slice thickness of 5mm. For each set of acquisition parameter image noise was measured and the Rose model observer was used to test two strategies for proposing a reasonable compromise between dose and low-contrast detection performance: (1) the use of a unique noise level for all test object diameters, and (2) the use of a unique dose efficacy level defined as the noise reduction per unit dose. Published data were used to define four weight classes and an acquisition protocol was proposed for each class. The protocols have been applied in clinical routine for more than one year. CTDIvol values of 6.7, 9.4, 15.9 and 24.5mGy were proposed for the following weight classes: 2.5-5, 5-15, 15-30 and 30-50kg with image noise levels in the range of 10-15HU. The proposed method allows patient dose and image noise to be controlled in such a way that dose reduction does not impair the detection of low-contrast lesions. The proposed values correspond to high- quality images and can be reduced if only high-contrast organs are assesse

Nutritional Asymmetries Are Related to Division of Labor in a Queenless Ant

Eusocial species exhibit pronounced division of labor, most notably between reproductive and non-reproductive castes, but also within non-reproductive castes via morphological specialization and temporal polyethism. For species with distinct worker and queen castes, age-related differences in behavior among workers (e.g. within-nest tasks versus foraging) appear to result from physiological changes such as decreased lipid content. However, we know little about how labor is divided among individuals in species that lack a distinct queen caste. In this study, we investigated how fat storage varied among individuals in a species of ant (Dinoponera australis) that lacks a distinct queen caste and in which all individuals are morphologically similar and capable of reproduction (totipotent at birth). We distinguish between two hypotheses, 1) all individuals are physiologically similar, consistent with the possibility that any non-reproductive may eventually become reproductive, and 2) non-reproductive individuals vary in stored fat, similar to highly eusocial species, where depletion is associated with foraging and non-reproductives have lower lipid stores than reproducing individuals. Our data support the latter hypothesis. Location in the nest, the probability of foraging, and foraging effort, were all associated with decreased fat storage

The gait and balance of patients with diabetes can be improved: a randomised controlled trial

Aims/hypothesis: Gait characteristics and balance are altered in diabetic patients. Little is known about possible treatment strategies. This study evaluates the effect of a specific training programme on gait and balance of diabetic patients. Methods: This was a randomised controlled trial (n = 71) with an intervention (n = 35) and control group (n = 36). The intervention consisted of physiotherapeutic group training including gait and balance exercises with function-orientated strengthening (twice weekly over 12weeks). Controls received no treatment. Individuals were allocated to the groups in a central office. Gait, balance, fear of falls, muscle strength and joint mobility were measured at baseline, after intervention and at 6-month follow-up. Results: The trial is closed to recruitment and follow-up. After training, the intervention group increased habitual walking speed by 0.149m/s (p < 0.001) compared with the control group. Patients in the intervention group also significantly improved their balance (time to walk over a beam, balance index recorded on Biodex balance system), their performance-oriented mobility, their degree of concern about falling, their hip and ankle plantar flexor strength, and their hip flexion mobility compared with the control group. After 6months, all these variables remained significant except for the Biodex sway index and ankle plantar flexor strength. Two patients developed pain in their Achilles tendon: the progression for two related exercises was slowed down. Conclusions/interpretation: Specific training can improve gait speed, balance, muscle strength and joint mobility in diabetic patients. Further studies are needed to explore the influence of these improvements on the number of reported falls, patients' physical activity levels and quality of life. Trial registration:: ClinicalTrials.gov NCT00637546 Funding:: This work was supported by the Swiss National Foundation (SNF): PBSKP-123446/

Virtual Noncontrast Abdominal Imaging with Photon-counting Detector CT.

Background Accurate CT attenuation and diagnostic quality of virtual noncontrast (VNC) images acquired with photon-counting detector (PCD) CT are needed to replace true noncontrast (TNC) scans. Purpose To assess the attenuation errors and image quality of VNC images from abdominal PCD CT compared with TNC images. Materials and Methods In this retrospective study, consecutive adult patients who underwent a triphasic examination with PCD CT from July 2021 to October 2021 were included. VNC images were reconstructed from arterial and portal venous phase CT. The absolute attenuation error of VNC compared with TNC images was measured in multiple structures by two readers. Then, two readers blinded to image reconstruction assessed the overall image quality, image noise, noise texture, and delineation of small structures using five-point discrete visual scales (5 = excellent, 1 = nondiagnostic). Overall image quality greater than or equal to 3 was deemed diagnostic. In a phantom, noise texture, spatial resolution, and detectability index were assessed. A detectability index greater than or equal to 5 indicated high diagnostic accuracy. Interreader agreement was evaluated using the Krippendorff α coefficient. The paired t test and Friedman test were applied to compare objective and subjective results. Results Overall, 100 patients (mean age, 72 years ± 10 [SD]; 81 men) were included. In patients, VNC image attenuation values were consistent between readers (α = .60), with errors less than 5 HU in 76% and less than 10 HU in 95% of measurements. There was no evidence of a difference in error of VNC images from arterial or portal venous phase CT (3.3 HU vs 3.5 HU, P = .16). Subjective image quality was rated lower in VNC images for all categories (all, P &lt; .001). Diagnostic quality of VNC images was reached in 99% and 100% of patients for readers 1 and 2, respectively. In the phantom, VNC images exhibited 33% higher noise, blotchier noise texture, similar spatial resolution, and inferior but overall good image quality (detectability index &gt;20) compared with TNC images. Conclusion Abdominal virtual noncontrast images from the arterial and portal venous phase of photon-counting detector CT yielded accurate CT attenuation and good image quality compared with true noncontrast images. © RSNA, 2022 Online supplemental material is available for this article See also the editorial by Sosna in this issue

Abyssal Atlantic circulation during the Last Glacial Maximum: Constraining the ratio between transport and vertical mixing

The ocean’s role in regulating atmospheric carbon dioxide on glacial‐interglacial timescales remains an unresolved issue in paleoclimatology. Reduced mixing between deep water masses may have aided oceanic storage of atmospheric CO_2 during the Last Glacial Maximum (LGM), but data supporting this idea have remained elusive. The δ^(13)C of benthic foraminifera indicate the Atlantic Ocean was more chemically stratified during the LGM, but the nonconservative nature of δ^(13)C complicates interpretation of the LGM signal. Here we use benthic foraminiferal δ^(18)O as a conservative tracer to constrain the ratio of meridional transport to vertical diffusivity in the deep Atlantic. Our calculations suggest that the ratio was at least twice as large at the LGM. We speculate that the primary cause was reduced mixing between northern and southern component waters, associated with movement of this water mass boundary away from the zone of intense mixing near the seafloor. The shallower water mass boundary yields an order of magnitude increase in the volume of southern component water, suggesting its residence time may have increased substantially. Our analysis supports the idea that an expanded volume of Antarctic Bottom Water and limited vertical mixing enhanced the abyssal ocean’s ability to trap carbon during glacial times