Transcriptional cytokine responses associated with pathological outcomes in chickens experimentally infected with pigeon variant of avian avulavirus type 1

Background: Newcastle disease (ND) caused by virulent avian avulavirus 1 (AAvV-1) within the family paramyxoviridae, affects variety of avian species around the globe as well as responsible for significant economic losses in the poultry industry.Methods: Transcriptional response of selected cytokines associated with pathological outcomes were studied on chickens after experimental infection with three virulent isolates of pigeon variant avian avulavirus type 1 (PAvV-1). The specific pathogen free (SPF) chickens were used in the study, after virus inoculation via the intranasal route virus, observed daily for clinical symptoms. On day 3 of post infection, the experiment was terminated and then anatomopathogical and histopathological changes, virus load and transcriptional cytokine response were determined in the spleen.Results: For the study, four cytokines were chosen: interferons (IFN-β, IFN-γ) and interleukins (IL-2, IL-6). The increased transcriptional levels of cytokine expression were associated with viral gene expression and marked lesions. The increase in the mRNA levels of IL-6 was most significant in chickens with clinical symptoms. The combined up-regulations of IFN-γ and IL-6 mRNA expression were detected simultaneously with infiltration of macrophages in the histopathology analysis. The RNA expression of IL-2 and slight up-regulation of IFN-β were only in chickens infected with isolate closely related to poultry isolates in the phylogenetic study.Conclusions: The difference in the mRNA expression levels of cytokines may help to elucidate mechanisms of immune responses in birds infected with different virulent strains of avian avulavirus type 1

Business Model of Culture and Creative Industries Incubator in creating social innovation

This project has been funded with support from the European Commission. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.The publication was co-funded by Culture programme of the European Union as a part of Campus Culturae project (grant No. 2011-1177/001-001)

Desenho de materiais funcionais 2D para futuras aplicações em microeletrónica

Doutoramento em Ciência e Engenharia de MateriaisDevido à redução de dimensões e ao aumento da velocidade de processamento de dados nos dispositivos microeletrónicos baseados em semicondutores convencionais, estão a ser exploradas abordagens inovadoras envolvendo novos materiais tais como óxidos funcionais. Com o rápido desenvolvimento da indústria eletrónica existe uma maior necessidade de elevado desempenho, de elevada fiabilidade, e de componentes eletrónicos miniaturizados integrados em vários dispositivos. A fim de tornar os dispositivos amplamente acessíveis e de fácil utilização, requisitos adicionais devem ser considerados: o tamanho e peso desejados, o custo reduzido, o baixo consumo de energia e a portabilidade. Materiais funcionais de baixa dimensionalidade são muito promissores para cumprir essas exigências. Em particular, os ferroeléctricos de filmes finos bidimensionais (2D) têm recebido grande atenção devido à sua crescente utilização em memórias não voláteis, detectores piroelétricos, transdutores piezoeléctricos miniaturizados e dispositivos sintonizáveis de micro-ondas. A temperatura de cristalização é um parâmetro chave na preparação de ferroelétricos 2D. Muitos filmes finos ferroelétricos são cristalizados a temperaturas >600 °C. Esses valores estão acima da temperatura que certos elementos do dispositivo funcional podem suportar. Recentemente, este facto tornou-se ainda mais importante, devido às promissoras aplicações que podem ser consideradas caso os ferroeléctricos 2D sejam compatíveis com substratos poliméricos flexíveis de baixo custo e de baixo ponto de fusão. A compatibilidade de filmes finos ferróicos com estes últimos tipos de substratos é muito difícil, mas se conseguida pode ampliar acentuadamente a gama de aplicações para os mais recentes requisitos de eletrónica flexível e microeletrónica, onde dispositivos leves e baratos são exigidos. Neste trabalho, é implementada uma combinação da modificação da química de precursores e assistência por luz UV, com promoção simultânea da cristalização pela introdução de sementes nanocristalinas na solução precursora, para a fabricação de filmes finos ferróicos sem chumbo - Método de Precursores Fotossensíveis Semeados. Neste contexto, o principal objetivo deste trabalho foi fabricar filmes finos sem chumbo BiFeO3 (BFO) e Na0.5Bi0.5TiO3 (NBT) a baixas temperaturas (~300 °C) com uma resposta ferroelétrica competitiva. Além disso, a investigação do efeito do elétrodo-base sobre as propriedades dielétricas e ferroelétricas de filmes finos de BFO foi levada a cabo, e a comparação entre o comportamento de condensadores de BFO com base em IrO2, LaNiO3 (LNO) e Pt foi estabelecida. Adicionalmente, os efeitos dos vários eléctrodos sobre a microestrutura de filmes finos ferroeléctricos de BFO foram estudados por microscopia eletrónica de transmissão (TEM) de alta resolução. Primeiramente, filmes finos finos de perovesquite BFO e NBT foram preparados sobre substratos de silício revestidos com Pt, por deposição de solução química. Os filmes finos de BFO foram preparados a temperaturas na gama de 400-500 °C, a partir de soluções de precursores estequiométricas e com excesso de Bi. Os filmes de BFO cristalinos foram obtidos a 400 °C, o limite inferior de temperatura. Os filmes preparadas com excesso de Bi possuem curvas de histerese ferroelétrica mais definidas do que aqueles sem qualquer excesso, para filmes com espessuras ~150 nm. Uma vez que as densidades de corrente de fuga nos filmes finos diminuem com a diminuição da temperatura de processamento, a polarização de filmes finos de BFO preparados com excesso Bi e recozidos a 400 e 450 °C pode ser efetivamente comutada à temperatura ambiente. Obtiveram-se valores de polarização remanescente de Pr ~10 e ~60 μC/cm2 com campos coercivos de EC ~ 205 e 235 kV/cm para os filmes finos preparados a 400 e 450 °C, respectivamente. Os filmes finos de NBT foram preparados a temperaturas entre 400 e 650 °C. As propriedades estruturais e ferroelétricas dos filmes foram examinadas. A constante dieléctrica observada e as perdas dieléctricas a 100 kHz são 616 e 0,032, respectivamente, enquanto que a polarização remanescente observada e o campo coercivo são Pr ~ 24 μC/cm2 e EC ~ 215 kV/cm, respectivamente para o filme de NBT recozido a 650 °C. O recozimento térmico, em atmosfera de oxigénio após cada camada de revestimento, é eficaz na promoção da cristalização do filme na fase de perovesquite romboédrica a uma baixa temperatura de 400 °C. No entanto, obteve-se um ciclo P-E quase linear para os filmes NBT cristalizados a 400 °C devido à sua incipiente cristalinidade. Os filmes finos de BFO foram depositados numa gama de elétrodos para determinar o seu papel no controlo da formação de fases e da microestrutura. A cristalização em elétrodos de óxido seguiu a sequência: amorfa → Bi2O2(CO3) → perovesquite, enquanto que nos elétrodos de Pt cristalizaram diretamente a partir da fase amorfa. Os elétrodos de IrO2 promoveram a formação da fase de perovesquite à temperatura mais baixa e o LNO induziu adicionalmente o crescimento epitaxial local. O LNO tem a estrutura de perovesquite com o parâmetro de rede a = 0.384 nm, compatível com o de BFO, a = 0.396 nm, e assim a epitaxia é mais provável. Todas as composições exibiram precipitados inteiramente coerentes ricos em Fe dentro do interior de grão da matriz de perovesquite, enquanto que a incoerente segunda fase de Bi2Fe4O9 foi também observada nos limites de grão de BFO crescido em eléctrodos de Pt. Esta última pode ser observada por difração de raios X, bem como TEM, mas os precipitados coerentes foram observados apenas por TEM, principalmente evidenciados pelo seu contraste Z em imagens de campo escuro anular. Estes dados têm consequências acentuadas permitindo alargar a utilização de filmes de BFO sob campo aplicado, a aplicações como atuadores, sensores e aplicações de memória. Em seguida, os filmes finos de BFO foram depositados em substratos de Si com elétrodos distintos, como Pt, LNO e IrO2, para investigar o efeito do elétrodo-base sobre o crescimento e as propriedades elétricas do BFO. Todas os filmes de BFO são compostos por grãos colunares cujo tamanho é dependente do elétrodo-base. Não se observou textura para filmes de 320 nm de espessura fabricados em Pt orientado (111). Os filmes sobre eléctrodos de óxido, em particular sobre LNO são altamente orientados no plano (012). A grande polarização remanescente em BFO/Pt e BFO/IrO2 é atribuída à alta contribuição de corrente de fuga. Os filmes BFO de 400 nm de espessura em LNO possuem uma baixa densidade de corrente de fuga ~4 × 10-6 A/cm2, uma grande polarização remanescente de 50 μC/cm2 e um pequeno campo coercitivo de 180 kV/cm à temperatura ambiente. Demonstramos que as camadas de LNO aumentam a cristalinidade e a orientação de filmes finos BFO, o que se reflete nas suas propriedades funcionais. Este estudo mostra que, além da simples necessidade de filmes monofásicos, os elétrodos de óxido de metal têm um impacto relevante no desenvolvimento de filmes finos BFO de alta qualidade fabricados por métodos químicos de deposição de solução. Estes resultados têm uma implicação grande para a fabricação de dispositivos BFO baseados em filmes finos. Finalmente, provamos que é possível fabricar diretamente filmes finos de BFO sem chumbo em substratos flexíveis de poliamida com funcionalidades ferroelétricas e magnéticas (multiferroicidade) à temperatura ambiente. O nosso método inovador, baseado em soluções de Precursores Fotossensíveis e nanosementes cristalinas, foi usado com sucesso para diminuir a temperatura de cristalização de filmes finos de BFO até uma temperatura tão baixa quanto 300 °C, a mais baixa temperatura reportada até agora para a preparação de filmes finos multiferróicos de BFO. Apesar deste excepcionalmente baixo nível térmico, obtém-se uma polarização remanescente Pr de 2.8 μC/cm2 para os filmes semeados + UV, com um campo coercitivo EC de 300 kV/cm. A estratégia de síntese baseada na utilização de precursores fotossensíveis sementados pode ser transferida para qualquer outra família de óxidos metálicos funcionais.With the dimensions reduction and data processing speeds increasing of conventional semiconductor based microelectronic devices, innovative approaches involving new materials such as functional oxides are being explored. With the rapid development of the electronics industry there is a need for high performance, high reliability and miniaturized electronic components integrated into various devices. In order to make the devices user friendly and widely accessible, additional requirements should be considered: the desired size and weight, low cost, low power consumption, and portability in addition to high levels of functionality. Low dimensional functional materials hold great promises to fulfil those requirements. In particular, two-dimensional (2D) thin film ferroelectrics have received wide attention because of their growing use as non-volatile memories, pyroelectric detectors, miniaturized piezoelectric transducers and tunable microwave devices. Crystallization temperature is a key parameter in preparation of 2D-ferroelectrics. Many ferroelectric thin films are crystallized at temperatures >600 °C. This is above the temperature that certain elements of the functional device can withstand. Recently it became even more important due to promising applications that can be envisaged if 2D-ferroelectrics will be compatible with low cost, low melting temperature flexible polymeric substrates. The compatibility of ferroic thin films with those last types of substrates can markedly widen the range of applications towards the most recent requirements of flexible electronics and microelectronics, where lightweight and cheap devices are demanded. In this work, a combination of the modification of precursor chemistry and the assistance of UV-light, with simultaneous promotion of crystallization by introducing nanocrystalline seeds in the precursor solution, is implemented to fabricate lead-free ferroic thin films - Seeded Photosensitive Precursor Method. Within this context, the main objective of this work was to fabricate lead-free BiFeO3 (BFO) and Na0.5Bi0.5TiO3 (NBT) thin films with a competitive ferroelectric response at low temperatures. Moreover, investigations of the effect of the bottom electrode on the dielectric and ferroelectric properties of BFO thin films was conducted and the comparison between the behavior of IrO2, LaNiO3 (LNO) and Pt based BFO capacitors established. Additionally, the effects of these various bottom electrodes on the microstructure of BiFeO3 ferroelectric films was studied by high-resolution TEM. Firstly, BFO and NBT perovskite thin films were prepared on Pt-coated silicon substrates by chemical solution deposition. BFO was prepared at temperatures in the range 400-500 °C, and from stoichiometric and Bi excess precursor solutions. Crystalline BFO films were obtained at the lowest temperature limit of 400 °C. The films prepared with Bi excess possess more defined ferroelectric hysteresis loops than those without any excess; for films with thicknesses ~150 nm. As the leakage current densities in the films decrease with decreasing the processing temperature, polarization of BFO films prepared with Bi excess and annealed at 400 and 450 °C can be effectively switched at room temperature. Remanent polarization values of Pr ~ 10 and ~60 μC/cm2 with coercive fields of EC ~ 205 and 235 kV/cm were obtained for the films prepared at 400 and 450 °C, respectively. NBT thin films were prepared at temperatures from 400 to 650 °C. Structural and ferroelectric properties of the films were examined. The observed dielectric constant and dielectric losses at 100 kHz are 616 and 0.032, respectively, while the observed remanent polarization and coercive field are Pr ~ 24 μC/cm2 and EC ~ 215 kV/cm, respectively for the NBT film annealed at 650 °C. Thermal annealing in an oxygen atmosphere after each layer of coating is effective in promoting crystallization of the film into rhombohedral perovskite phase at a low temperature of 400 °C. However, almost linear, P-E loop was obtained for those NBT films crystallized at 400 °C due to incipient crystallinity. BFO thin films were grown on a range of electrodes to determine their role in controlling phase formation and microstructure. The crystallization on oxide electrodes followed the sequence: amorphous → Bi2O2(CO3) → perovskite, while those on Pt crystallized directly from the amorphous phase. IrO2 electrodes promoted perovskite phase formation at the lowest temperature and LaNiO3 additionally induced local epitaxial growth. LNO has the perovskite structure with lattice parameter a = 0.384 nm, compatible with that of BFO, a = 0.396 nm and thus epitaxy is more likely. It was observed for the first time that all compositions exhibited fully coherent Fe-rich precipitates within the grain interior of the perovskite matrix, whereas incoherent Bi2Fe4O9 second phase was also observed at the grain boundaries of BFO grown on Pt electrodes. The latter could be observed by X-ray diffraction as well as transmission electron microscopy (TEM) but coherent precipitates were only observed by TEM, principally evidenced by their Z contrast in annular dark field images. These data have pronounced consequences for the extended use of BFO films under applied field for actuator, sensor and memory applications. Then, BFO thin films were deposited on Si-based substrates with distinct electrodes, such as Pt, LNO, and IrO2, in order to investigate the effect of bottom electrode on the growth and electrical properties of BFO. All BFO films are composed of columnar grains which size is dependent on the bottom electrode. No texture was observed for 320 nm thick films fabricated on (111) oriented Pt. Films on oxide electrodes, in particular on LNO are highly (012) oriented. The large remanent polarization in BFO/Pt and BFO/IrO2 is attributed to the high leakage current contribution. 400 nm thick BFO films on LNO possess a low leakage current density ~4 × 10-6 A/cm2, a large remanent polarization of 50 μC/cm2 and a small coercive field of 180 kV/cm at room temperature. We demonstrate that LNO layers enhance the crystallinity and orientation of BFO thin films, which is reflected in their functional properties. This study shows that besides the simple need of monophasic films metal oxide electrodes have a relevant impact on the development of high quality BFO thin films fabricated by chemical solution deposition methods. These results have a broad implication for the fabrication of BFO thin film based devices. Finally, we prove that it is possible to directly fabricate lead-free BFO thin films on flexible polyamide substrates with ferroelectric and magnetic functionalites (multiferroicity) at room temperature. Our own proprietary novel solution-based Seeded Photosensitive Precursor Method was successfully used to decrease the crystallization temperature of BFO thin films down to a temperature as low as 300 °C, the lowest reported up to now for the preparation of multiferroic BFO thin films. Despite this exceptionally low thermal budget a remanent polarization Pr of 2.8 μC/cm2 is obtained for the seeded + UV films, with a coercive field EC of 300 kV/cm. The synthesis strategy based on the use of seeded photosensitive precursors can be transferred to any family of functional metal oxide

Antimicrobial and antifungal activities of the extracts and essential oils of Bidens tripartita.

The aim of this study was to determine the antibacterial and antifungal properties of the extracts, subextracts and essential oils of Bidens tripartita flowers and herbs. In the study, twelve extracts and two essential oils were investigated for activity against different Gram-positive Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus, Gram-negative bacteria Escherichia coli, E. coli (beta-laktamase+), Klebsiella pneumoniae (ESBL+), Pseudomonas aeruginosa and some fungal organisms Candida albicans, C. parapsilosis, Aspergillus fumigatus, A. terreus using a broth microdilution and disc diffusion methods. The results obtained indicate antimicrobial activity of the tested extracts (except butanolic extracts), which however did not inhibit the growth of fungi used in this study. Bacteriostatic effect of both essential oils is insignificant, but they have strong antifungal activity. These results support the use of B. tripartita to treat a microbial infections and it is indicated as an antimicrobial and antifungal agent, which may act as pharmaceuticals and preservatives

Studies of new antiferroelectric liquid crystal based on quantum-chemical model

Physical properties of new thermotropic antiferroelectric liquid crystal have been studied. Experiments were done by use of complementary methods such as differential scanning calorimetry, polarizing optical microscopy and X-ray powder diffractometry. Acquired data from X-ray powder diffractometry was examined under application of quantum chemical approach. It has been found that compound studied exhibits stable enantiotropic antiferroelectric SmC_{A}^{*} phase in the wide temperature range while ferroelectric phase SmC^{*} is very narrow

Influence of vegetarian diet on human body

All over the world there are more vegetarians than in previous years due to many reasons. One of them is positive influence on health what is shown in the latest research. The aim of the article is to review the influence of vegetarian diet on frequent diseases such as: hypertension, obesity,osteoporosis.An analysis of scientific papers from Pubmed and Google Scholar was performed, which most accurately described the issue of impact vegetarian diet on human health. The following keywords have been used in the search:Vegetarian, hypertension, obesity, osteoporosis:vegetarian, hypertension, obesity, osteoporosis.The researchers shown than vegetarians have lover systolic blood pressure  and diastolic blood pressure by 10 and 5 mmHg, they also lower overall mortality by 10-15%, risk of heart failure and by ~40%,  stroke by~35% and coronary events by 20%. Vegetarians also have statistically  lower body mass index. What’s more in this diet is more energy efficient thanks too low food energy density and helps keeping leptin on adequate level. Research has shown than vegetarians have lower mineral bone density than non-vegetarian, although some studies has shown that  risk of fractures in vegetarians and non-vegetarians was not affected by diet alternation. Based on the results of the studies above vegetarian diet contributes to lower blood pressure and preventing from cardiovascular diseases. It helps to avoid obesity and  to get rid of weights also doesn’t have negative impact on osteoporosis

Response to a DNA vaccine against the H5N1 virus depending on the chicken line and number of doses

Background Avian influenza virus infections cause significant economic losses on poultry farms and pose the threat of a possible pandemic outbreak. Routine vaccination of poultry against avian influenza is not recommended in Europe, however it has been ordered in some other countries, and more countries are considering use of the avian influenza vaccine as a component of their control strategy. Although a variety of such vaccines have been tested, most research has concentrated on specific antibodies and challenge experiments. Methods We monitored the transcriptomic response to a DNA vaccine encoding hemagglutinin from the highly pathogenic H5N1 avian influenza virus in the spleens of broiler and layer chickens. Moreover, in layer chickens the response to one and two doses of the vaccine was compared. Results All groups of birds immunized with two doses of the vaccine responded at the humoral level by producing specific anti-hemagglutinin antibodies. A response to the vaccine was also detected in the spleen transcriptomes. Differential expression of many genes encoding noncoding RNA and proteins functionally connected to the neuroendocrine-immune system was observed in different immunized groups

Charakterystyka postawy ciała dzieci w wieku przedszkolnym regionu bydgoskiego = Characteristics of body posture of Bydgoszcz region pre-school children

Mieszkowska Monika, Kochanowicz Magdalena, Garbin Michał, Srokowski Grzegorz, Tomczyk Maja, Maciejewski Jerzy. Charakterystyka postawy ciała dzieci w wieku przedszkolnym regionu bydgoskiego =Characteristics of body posture of Bydgoszcz region pre-school children. Journal of Education, Health and Sport. 2016;6(8):637-652. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.61120http://ojs.ukw.edu.pl/index.php/johs/article/view/3795   The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 755 (23.12.2015).755 Journal of Education, Health and Sport eISSN 2391-8306 7© The Author (s) 2016;This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, PolandOpen Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium,provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License(http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted, non commercialuse, distribution and reproduction in any medium, provided the work is properly cited.The authors declare that there is no conflict of interests regarding the publication of this paper.Received: 05.08.2016. Revised 25.08.2016. Accepted: 28.08.2016.  Charakterystyka postawy ciała dzieci w wieku przedszkolnym regionu bydgoskiegoCharacteristics of body posture of Bydgoszcz region pre-school children Monika Mieszkowskaa, Magdalena Kochanowicz b, Michał Garbin c, Grzegorz Srokowski c, Maja Tomczyk b, Jerzy Maciejewskid a Poznan University of Medical Sciences, Poznań, Poznan, Poland  b Jędrzej Śniadecki Academy of Physical Education and Sport in Gdańsk, Gdańsk, Polandc Nicolaus Copernicus University Ludwik Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland`d Multi-Municipal Hospital. Dr. E. Warminski in Bydgoszcz, Bydgoszcz, Poland  Key words: faulty postures, body mass index, Lovett testing, corrective gymnastics,Słowa kluczowe: wady postawy, BMI, testowanie Lovetta, gimnastyka korekcyjna, AbstractIntroduction. The problem of postures faults concerns on the increasing number of children at younger age and significantly affecting their health. Early diagnosis and its correction allow achieving measurable effects influencing on the quality of life in adulthood. The aim of the study was to assess the selected posture parameters of preschool children. Furthermore the aim was to determine their impact of postures faults on the muscle strength of selected muscle groups.Material/Methods. 50 preschool children, 23 girls and 27 boys (average age 5.34 (± 1.09) years) attending to a non-public kindergarten "Ludzikowo" in Bydgoszcz were examined. The study was conducted in the period from January to March 2013 and consisted of three parts: a questionnaire (diagnostic survey), anthropometric survey (weight, height, BMI), visual analyzes - qualitative method (comparison of posture to the reference), assessment of muscle strength of selected muscle groups using a Lovett testing.Results. The study group showed abnormalities at least in one element of the body posture. In the anterior and lateral projection 90%, in the rear projection 96% of examined children showed the presence of posture faults. A comprehensive body structure and posture analysis according to Staffel method showed that only 62% qualify for the so-called normal pattern of body posture. No statistically significant differences in muscle strength were found in whole group.Conclusions. Utility and the simplicity of proposed assessing methods enables their widespread and early uses in preschool children body posture analyzes. The presence of postures faults in preschool age children does not affect their level of muscle strength.  StreszczenieWstęp: Problem wad postawy dotyczy coraz większej liczby dzieci w coraz to młodszym wieku i znacząco rzutuje na ich zdrowie. Wczesna diagnostyka oraz ich korekcja pozwala uzyskać wymierne efekty terapeutyczne znacząco niwelując szkodliwość występujących wad oraz ich wpływ na komfort życia w dorosłości. Celem badania była ocena wybranych parametrów postawy ciała dzieci w wieku przedszkolnym oraz określenie wpływu występujących wad na poziom siły mięśniowej wybranych grup mięśni wg. skali Lovetta.Materiał i metody: Badania obejmowały grupę 50 dzieci, 23 dziewcząt i 27 chłopców (w wieku 5,34 (± 1,09) lata), uczęszczających do niepublicznego przedszkola „Ludzikowo” w Bydgoszczy. Badania przeprowadzono w okresie od stycznia do marca 2013. Badanie obejmowało trzy części: kwestionariusz ankiety (met. sondażu diagnostycznego), badanie antropometryczne (pomiar masy ciała, wzrostu, BMI), badanie wzrokowe - metoda jakościowa (porównanie postawy swobodnej z wzorcem), ocena siły mięśniowej wybranych grup mięśniowych przy pomocy testu Lovetta. Wyniki: Grupa badana wykazywała odchylenia od normy w przynajmniej jednym z obserwowanych elementów postawy ciała. W projekcji przedniej oraz bocznej u 90% dzieci wykazano obecność zaburzeń postawy. W badaniu w projekcji tylnej u 96% badanych wystąpiły odchylenia od prawidłowych wzorców postawy ciała. Całościowa analiza budowy i postawy ciała wg Staffela wykazała, że jedynie 62% kwalifikuje się do tzw. normalnego wzorca postawy ciała. Mimo zaburzeń wzorców postawy nie stwierdzono istotnych statystycznie zaburzeń siły mięśniowej wg. Lovetta.Wnioski: Użyteczność oraz łatwość wykonania zaproponowanych metody oceny postawy ciała pozwala na ich bardzo szerokie zastosowanie i umożliwia wczesną diagnostykę posturalną dzieci przedszkolnych. Obecność wad postawy w wieku przedszkolnym nie rzutuje jeszcze na poziom siły mięśniowej wybranych grup mięśniowych