Extraction of antioxidant phenolic compounds by autohydrolysis of spent coffee grounds

The extraction of antioxidant phenolic compounds from spent coffee grounds (SCG) was studied. Experimental assays were carried out by the autohydrolysis technique and the effect of the process variables temperature, water/SCG ratio and extraction time on the amount of phenolic compounds and antioxidant activity of the produced extracts was evaluated. The variables were combined according to a 23 central composite design. The statistical model revealed that the optimum condition to produce extracts with high content of phenolic compounds (35.07 mg GAE/g SCG) and antioxidant activity (FRAP = 0.25 mmol Fe(II)/g SCG, DPPH = 121.7 μmol TE/g SCG, ABTS = 130.0 μmol TE/g SCG, TAA = 64.17 mg α-TOC/g SCG) was achieved when maximizing the process variables (temperature = 200 °C, ratio = 15 ml/g and time = 50 min)

Knowledge and Impact of COVID-19 on Middle-Aged and Older People Living with HIV in Lima, Peru

COVID-19 has had an unprecedented worldwide impact, and Peru has had one of the highest COVID-19 case rates despite implementation of an early strict nationwide quarantine. Repercussions on Peru's healthcare system may impact vulnerable populations, particularly people with HIV (PWH). We explored knowledge of COVID-19 and the socioeconomic and health impact of the pandemic among middle-aged and older PWH. A cross-sectional telephone survey was administered to 156 PWH age ≥40 years receiving care in one of two large HIV clinics in Lima, Peru. The majority of PWH (age 52 ± 7.7 years, 41% female, 65% completed secondary school or less) were knowledgeable regarding COVID-19 symptoms and prevention methods. Nearly half of those employed prior to the pandemic reported job loss. Female sex (unadjusted prevalence ratio [PR] 1.85 [95%CI 1.27-2.69]), low educational level (PR 1.62 [1.06-2.48]) and informal work (PR 1.58 [1.06-2.36]) were risk factors for unemployment but not in adjusted models. Increased anxiety was reported in 64% and stress in 77%. COVID-19 has had a substantial socioeconomic and mental health impact on PWH living in Lima, Peru, particularly those with lower educational levels and informal workers. Efforts are needed to ensure continued medical care and socioeconomic support of PWH in Peru

Cancer Survivors\u27 Self-Efficacy and Spirituality Outcomes Following a Holistic Integrative Intervention

Cancer treatments often negatively impact health-related quality of life (HR-QOL) for cancer survivors (CS), ignoring the multi-dimensional nature of the human experience of cancer and its impact on mental and spiritual domains. A holistic integrative approach was implemented on a heterogenous population of cancer survivors during the COVID epidemic with the goal of improving their overall well-being by looking beyond physical functioning. PURPOSE: To examine the relationship between spirituality and self-efficacy of cancer survivors of all types of cancer following a holistic intervention during the COVID-19 pandemic. METHODS: Subjects were recruited via network partners and self-referral. Subjects were eligible to participate if they were cancer survivors of any type at any stage of cancer treatment; subjects were ineligible to participate if they presented with any absolute contraindications to exercise testing as per ACSM guidelines. For 16 weeks, subjects were asked to participate in three 75-minute sessions of therapeutic yoga-based with loving kindness meditation per week. Psychosocial support text messages were sent daily to subjects based on their motivational state to encourage participation in these health enhancing behaviors. Data was collected through numerous channels including BlueJay Mobile Telehealth medicine application. RESULTS: A total of 29 survivors provided informed consent. The average age of the subjects was 58.9 years, 25 female survivors and 4 male survivors; 11 of the 29 self-reported as Latino/ Hispanic. The 29 subjects were survivors of the following primary cancers: breast cancer (n=21), cervical cancer (n=2), ovarian cancer (n=1), prostate cancer (n=1), sarcoma (n=1), lymphoma (n=1), thyroid cancer (n=1), or leukemia (n=1). There is a positive association between how confident a cancer survivor feels towards performing exercise in various situations and the amount of social support they receive from their family (p\u3c.001) and friends (p\u3c.001). The individual’s confidence to overcome the obstacle to exercise and their level of hope are associated with the support they receive from loved ones. CONCLUSION: The present study suggests that to improve exercise behavior in adult cancer survivors, one should incorporate social support to strengthen barriers self-efficacy to improve outcome expectations. To further understand these associations, longitudinal research is needed and should include more survivors

Rap1 binding and a lipid-dependent helix in talin F1 domain promote integrin activation in tandem.

Rap1 GTPases bind effectors, such as RIAM, to enable talin1 to induce integrin activation. In addition, Rap1 binds directly to the talin1 F0 domain (F0); however, this interaction makes a limited contribution to integrin activation in CHO cells or platelets. Here, we show that talin1 F1 domain (F1) contains a previously undetected Rap1-binding site of similar affinity to that in F0. A structure-guided point mutant (R118E) in F1, which blocks Rap1 binding, abolishes the capacity of Rap1 to potentiate talin1-induced integrin activation. The capacity of F1 to mediate Rap1-dependent integrin activation depends on a unique loop in F1 that has a propensity to form a helix upon binding to membrane lipids. Basic membrane-facing residues of this helix are critical, as charge-reversal mutations led to dramatic suppression of talin1-dependent activation. Thus, a novel Rap1-binding site and a transient lipid-dependent helix in F1 work in tandem to enable a direct Rap1-talin1 interaction to cause integrin activation

Highly Pathogenic H5N1 Avian Influenza: Entry Pathways into North America via Bird Migration

Given the possibility of highly pathogenic H5N1 avian influenza arriving in North America and monitoring programs that have been established to detect and track it, we review intercontinental movements of birds. We divided 157 bird species showing regular intercontinental movements into four groups based on patterns of movement—one of these groups (breed Holarctic, winter Eurasia) fits well with the design of the monitoring programs (i.e., western Alaska), but the other groups have quite different movement patterns, which would suggest the importance of H5N1 monitoring along the Pacific, Atlantic, and Gulf coasts of North America

B Cells Migrate into Remote Brain Areas and Support Neurogenesis and Functional Recovery after Focal Stroke in Mice

Lymphocytes infiltrate the stroke core and penumbra and often exacerbate cellular injury. B cells, however, are lymphocytes that do not contribute to acute pathology but can support recovery. B cell adoptive transfer to mice reduced infarct volumes 3 and 7 d after transient middle cerebral artery occlusion (tMCAo), independent of changing immune populations in recipient mice. Testing a direct neurotrophic effect, B cells cocultured with mixed cortical cells protected neurons and maintained dendritic arborization after oxygen-glucose deprivation. Whole-brain volumetric serial two-photon tomography (STPT) and a custom-developed image analysis pipeline visualized and quantified poststroke B cell diapedesis throughout the brain, including remote areas supporting functional recovery. Stroke induced significant bilateral B cell diapedesis into remote brain regions regulating motor and cognitive functions and neurogenesis (e.g., dentate gyrus, hypothalamus, olfactory areas, cerebellum) in the whole-brain datasets. To confirm a mechanistic role for B cells in functional recovery, rituximab was given to human CD20+ (hCD20+) transgenic mice to continuously deplete hCD20+-expressing B cells following tMCAo. These mice experienced delayed motor recovery, impaired spatial memory, and increased anxiety through 8 wk poststroke compared to wild type (WT) littermates also receiving rituximab. B cell depletion reduced stroke-induced hippocampal neurogenesis and cell survival. Thus, B cell diapedesis occurred in areas remote to the infarct that mediated motor and cognitive recovery. Understanding the role of B cells in neuronal health and disease-based plasticity is critical for developing effective immune-based therapies for protection against diseases that involve recruitment of peripheral immune cells into the injured brain

Erratum: Author Correction: Identification of genes required for eye development by high-throughput screening of mouse knockouts.

[This corrects the article DOI: 10.1038/s42003-018-0226-0.]

Outbreak of Antiviral Drug–Resistant Influenza A in Long-Term Care Facility, Illinois, USA, 2008

An outbreak of oseltamivir-resistant influenza A (H1N1) occurred in a long-term care facility. Eight (47%) of 17 and 1 (6%) of 16 residents in 2 wards had oseltamivir-resistant influenza A virus (H1N1) infections. Initial outbreak response included treatment and prophylaxis with oseltamivir. The outbreak abated, likely because of infection control measures