Parc de la riera del Palau a Terrassa

Situat a la ciutat de Terrassa el projecte té com a objectiu millorar la connexió dels barris de Les Fonts i de Can Parellada, situats a l’extrem sud del terme municipal, amb els altres barris de la ciutat a través dels camins que voregen la riera del Palau, i que juntament amb el seu entorn, constitueixen el Parc de la Riera del Palau. El projecte proposa la renaturalització de la riera, l’adequació dels camins, noves passeres, la millora de l’entorn, i una reorganització i proposta d’espais d’horta, de lleure i descans, donant valor a aquest espai de la ciutat

Parc de la riera del Palau a Terrassa

Situat a la ciutat de Terrassa el projecte té com a objectiu millorar la connexió dels barris de Les Fonts i de Can Parellada, situats a l’extrem sud del terme municipal, amb els altres barris de la ciutat a través dels camins que voregen la riera del Palau, i que juntament amb el seu entorn, constitueixen el Parc de la Riera del Palau. El projecte proposa la renaturalització de la riera, l’adequació dels camins, noves passeres, la millora de l’entorn, i una reorganització i proposta d’espais d’horta, de lleure i descans, donant valor a aquest espai de la ciutat

Parc de la riera del Palau a Terrassa

Situat a la ciutat de Terrassa el projecte té com a objectiu millorar la connexió dels barris de Les Fonts i de Can Parellada, situats a l’extrem sud del terme municipal, amb els altres barris de la ciutat a través dels camins que voregen la riera del Palau, i que juntament amb el seu entorn, constitueixen el Parc de la Riera del Palau. El projecte proposa la renaturalització de la riera, l’adequació dels camins, noves passeres, la millora de l’entorn, i una reorganització i proposta d’espais d’horta, de lleure i descans, donant valor a aquest espai de la ciutat

Thermally-controlled extrusion-based bioprinting of collagen

PubMedID: 31041538Thermally-crosslinked hydrogels in bioprinting have gained increasing attention due to their ability to undergo tunable crosslinking by modulating the temperature and time of crosslinking. In this paper, we present a new bioink composed of collagen type-I and Pluronic® F-127 hydrogels, which was bioprinted using a thermally-controlled bioprinting unit. Bioprintability and rheology of the composite bioink was studied in a thorough manner in order to determine the optimal bioprinting time and extrusion profile of the bioink for fabrication of three-dimensional (3D) constructs, respectively. It was observed that collagen fibers aligned themselves along the directions of the printed filaments after bioprinting based on the results on an anisotropy study. Furthermore, rat bone marrow-derived stem cells (rBMSCs) were bioprinted in order to determine the effect of thermally-controlled extrusion process. In vitro viability and proliferation study revealed that rBMSCs were able to maintain their viability after extrusion and attached to collagen fibers, spread and proliferated within the constructs up to seven days of culture. [Figure not available: see fulltext.]. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.National Science Foundation: CMMI 1462232 Osteology Foundation: 15–042 BIDEP 2219 Türkiye Bilimsel ve Teknolojik Araştirma KurumuAcknowledgements This work was supported by the National Science Foundation Award (CMMI 1462232) and Osteology Foundation Award # 15–042. Dr. Veli Ozbolat acknowledges the support from the International Postdoctoral Research Scholarship Program (BIDEP 2219) of the Scientific and Technological Research Council of Turkey (TUBITAK). The authors would like to thank Dr. Albert Ratner (Mechanical and Industrial Engineering Department, University of Iowa, Iowa City, USA) providing the FLIR thermal camera system. The authors would like to thank Ethan M. Gerhard, Dr. Jian Yang and Kevin P. Godzik (Department of Biomedical Engineering, Penn State University, University Park, USA) for their assistance with the mechanical testing and the rheology study, respectively. The authors would like to also thank Mrs. Alyssa Sipos with her assistance to PSR and immunofluorescence staining. There has been no significant financial support for this work that could have influenced its outcome

Essential steps in bioprinting: From pre- to post-bioprinting

PubMedID: 29909085An increasing demand for directed assembly of biomaterials has inspired the development of bioprinting, which facilitates the assembling of both cellular and acellular inks into well-arranged three-dimensional (3D) structures for tissue fabrication. Although great advances have been achieved in the recent decade, there still exist issues to be addressed. Herein, a review has been systematically performed to discuss the considerations in the entire procedure of bioprinting. Though bioprinting is advancing at a rapid pace, it is seen that the whole process of obtaining tissue constructs from this technique involves multiple-stages, cutting across various technology domains. These stages can be divided into three broad categories: pre-bioprinting, bioprinting and post-bioprinting. Each stage can influence others and has a bearing on the performance of fabricated constructs. For example, in pre-bioprinting, tissue biopsy and cell expansion techniques are essential to ensure a large number of cells are available for mass organ production. Similarly, medical imaging is needed to provide high resolution designs, which can be faithfully bioprinted. In the bioprinting stage, compatibility of biomaterials is needed to be matched with solidification kinetics to ensure constructs with high cell viability and fidelity are obtained. On the other hand, there is a need to develop bioprinters, which have high degrees of freedom of movement, perform without failure concerns for several hours and are compact, and affordable. Finally, maturation of bioprinted cells are governed by conditions provided during the post-bioprinting process. This review, for the first time, puts all the bioprinting stages in perspective of the whole process of bioprinting, and analyzes their current state-of-the art. It is concluded that bioprinting community will recognize the relative importance and optimize the parameter of each stage to obtain the desired outcomes. © 2018 Elsevier Inc.National Science Foundation: 1600118, BIDEP 2219This work has been supported by National Science Foundation Award # 1600118 awarded to I.T.O. The authors also acknowledge Department of Science and Technology , Government of India, INSPIRE Faculty Award to P.D. The authors are grateful to International Postdoctoral Research Scholarship Program ( BIDEP 2219 ) of the Scientific and Technological Research Council of Turkey for providing scholarship to V. O and the support from the Turkish Ministry of National Education for providing graduate scholarship to B. A. The authors confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome

3D printing of poly(?-caprolactone)/poly(D,L-lactide-co-glycolide)/hydroxyapatite composite constructs for bone tissue engineering

Three-dimensional (3D) printing technology is a promising method for bone tissue engineering applications. For enhanced bone regeneration, it is important to have printable ink materials with appealing properties such as construct interconnectivity, mechanical strength, controlled degradation rates, and the presence of bioactive materials. In this respect, we develop a composite ink composed of polycaprolactone (PCL), poly(D,L-lactide-co-glycolide) (PLGA), and hydroxyapatite particles (HAps) and 3D print it into porous constructs. In vitro study revealed that composite constructs had higher mechanical properties, surface roughness, quicker degradation profile, and cellular behaviors compared to PCL counterparts. Furthermore, in vivo results showed that 3D-printed composite constructs had a positive influence on bone regeneration due to the presence of newly formed mineralized bone tissue and blood vessel formation. Therefore, 3D printable ink made of PCL/PLGA/HAp can be a highly useful material for 3D printing of bone tissue constructs. © Materials Research Society 2018

Collagen-infilled 3D printed scaffolds loaded with miR-148b-transfected bone marrow stem cells improve calvarial bone regeneration in rats

Differentiation of progenitors in a controlled environment improves the repair of critical-sized calvarial bone defects; however, integrating micro RNA (miRNA) therapy with 3D printed scaffolds still remains a challenge for craniofacial reconstruction. In this study, we aimed to engineer three-dimensional (3D) printed hybrid scaffolds as a new ex situ miR-148b expressing delivery system for osteogenic induction of rat bone marrow stem cells (rBMSCs) in vitro, and also in vivo in critical-sized rat calvarial defects. miR-148b-transfected rBMSCs underwent early differentiation in collagen-infilled 3D printed hybrid scaffolds, expressing significant levels of osteogenic markers compared to non-transfected rBMSCs, as confirmed by gene expression and immunohistochemical staining. Furthermore, after eight weeks of implantation, micro-computed tomography, histology and immunohistochemical staining results indicated that scaffolds loaded with miR-148b-transfected rBMSCs improved bone regeneration considerably compared to the scaffolds loaded with non-transfected rBMSCs and facilitated near-complete repair of critical-sized calvarial defects. In conclusion, our results demonstrate that collagen-infilled 3D printed scaffolds serve as an effective system for miRNA transfected progenitor cells, which has a promising potential for stimulating osteogenesis and calvarial bone repair. © 2019 Elsevier B.V.National Science Foundation: 1600118 National Institutes of Health: RDE024790A Osteology Foundation: 15-042 BIDEP 2219 International Team for Implantology: 1275_2017 Türkiye Bilimsel ve Teknolojik Araştirma KurumuThis research was funded by Osteology Foundation ( 15-042 ), International Team for Implantology (1275_2017) , and National Science Foundation ( 1600118 ) and National Institutes of Health ( RDE024790A ). Dr. R. Seda Tigli Aydin acknowledges a grant provided by the Scientific and Technological Research Council of Turkey (TUBITAK) ( BIDEP 2219 ). The authors are also thankful to The Huck Institute of The Life Sciences and Materials Research Institute at the Pennsylvania State University in providing support for the core facility use

Three-dimensional bioprinting using self-assembling scalable scaffold-free “tissue strands” as a new bioink

Recent advances in bioprinting have granted tissue engineers the ability to assemble biomaterials, cells, and signaling molecules into anatomically relevant functional tissues or organ parts. Scaffold-free fabrication has recently attracted a great deal of interest due to the ability to recapitulate tissue biology by using self-assembly, which mimics the embryonic development process. Despite several attempts, bioprinting of scale-up tissues at clinically-relevant dimensions with closely recapitulated tissue biology and functionality is still a major roadblock. Here, we fabricate and engineer scaffold-free scalable tissue strands as a novel bioink material for robotic-assisted bioprinting technologies. Compare to 400 μm-thick tissue spheroids bioprinted in a liquid delivery medium into confining molds, near 8 cm-long tissue strands with rapid fusion and self-assemble capabilities are bioprinted in solid form for the first time without any need for a scaffold or a mold support or a liquid delivery medium, and facilitated native-like scale-up tissues. The prominent approach has been verified using cartilage strands as building units to bioprint articular cartilage tissue

3D printing of poly(epsilon-caprolactone)/poly(D,L-lactide-co-glycolide)/hydroxyapatite composite constructs for bone tissue engineering

WOS: 000440179300005Three-dimensional (3D) printing technology is a promising method for bone tissue engineering applications. For enhanced bone regeneration, it is important to have printable ink materials with appealing properties such as construct interconnectivity, mechanical strength, controlled degradation rates, and the presence of bioactive materials. In this respect, we develop a composite ink composed of polycaprolactone (PCL), poly(D,L-lactide-co-glycolide) (PLGA), and hydroxyapatite particles (HAps) and 3D print it into porous constructs. In vitro study revealed that composite constructs had higher mechanical properties, surface roughness, quicker degradation profile, and cellular behaviors compared to PCL counterparts. Furthermore, in vivo results showed that 3D-printed composite constructs had a positive influence on bone regeneration due to the presence of newly formed mineralized bone tissue and blood vessel formation. Therefore, 3D printable ink made of PCL/PLGA/HAp can be a highly useful material for 3D printing of bone tissue constructs.National Science FoundationNational Science Foundation (NSF) [1600118]; Osteology Foundation [15-042]; International Postdoctoral Research Scholarship Program of the Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [BIDEP 2219]This work was partially supported by the National Science Foundation Award No. 1600118 and Osteology Foundation Award No. 15-042. The authors are thankful to Dr. Wu Yang for his assistance with the histology study. Dr. Veli Ozbolat acknowledges the support from the International Postdoctoral Research Scholarship Program (BIDEP 2219) of the Scientific and Technological Research Council of Turkey (TUBITAK). The authors are also thankful to Materials Research Institute at the Pennsylvania State University in supporting the X-ray scattering experiment. The authors also thank Dr. Abhishek Shetty from Anton-Paar USA, Inc. for his assistance with the rheology experiments. The authors confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome