Student awareness of the Daily News-Record

The Daily News-Record is a local daily newspaper located in Harrisonburg, Virginia, a city with a population of about 52,000. The Daily News-Record\u27s print circulation is 26,000, while its digital circulation is 800. The paper also houses other local publications, such as the Rocktown Weekly, a free weekly publication that covers local news and the local music scene. James Madison University is a mid-sized public university also located in Harrisonburg, Virginia. The researcher wanted to understand JMU student awareness of local news sources, student consumption of news sources, student intention to read the local paper and whether there was a relationship with student community involvement, and student perception of campus opinion leaders. The survey research resulted in these major findings: 1) JMU students were mostly unaware of local news sources, but among local news sources, were most aware of the Daily News-Record; 2) students rely on online sources the most (Internet, Facebook, etc.) for gathering news; 3) students trust professors most as campus opinion leaders; 4) very few students had newspaper subscriptions, but when they did, their purpose of having one was to receive local news; 5) students were, by a very small margin, more likely to read and subscribe to the Rocktown Weekly compared to the Daily News-Record; 6) students more involved in the community were more aware of and more likely to read the Daily News-Record and Rocktown Weekly; 7) students more involved in the community had a higher intention to read and subscribe to the Daily News-Record and Rocktown Weekly; and 8) students who rely on print newspapers and print newspaper websites had a higher intention to read and subscribe to the Daily News-Record and Rocktown Weekly. Based on these primary research findings, goals, objectives, strategies, and tactics were created for a potential future campaign for Daily News-Record staff members to implement. A key public that would be targeted in this campaign was identified as a result of the research findings. This public has been named the Community Oriented Traditional Media Consumers (COTMC). This student public consumes more traditional forms of media, such as newspapers and newspaper websites, and is heavily involved in their community. Members of this public are more likely to read and subscribe to both publications, and students overall were more likely to subscribe to the Rocktown Weekly, so the COTMC public would be targeted in a campaign to increase their readership of the Rocktown Weekly. JMU students as a whole would also be targeted in this campaign, but because their intentions to read and subscribe to the publications were low, they would be targeted in a campaign to increase their overall awareness of both publications

Oral glutamine attenuates indomethacin-induced small intestinal damage

The use of NSAIDs (non-steroidal anti-inflammatory drugs), although of great therapeutic value clinically, is limited by their tendency to cause mucosal damage in the gastrointestinal tract. In the small intestine, the effects these drugs have been shown to produce include inhibition of cyclo-oxygenase, mitochondrial dysfunction and free radical-induced oxidative changes, all of which contribute to the mucosal damage seen. Glutamine is a fuel preferentially used by enterocytes and is known to contribute to maintaining the integrity of these cells. In the present study, we investigated the effect of glutamine on indomethacin-induced changes in the small intestinal mucosa. Rats were given 2% glutamine or glutamic acid or isonitrogenous amino acids, glycine or alanine, in the diet for 7 days. Indomethacin was then administered orally at a dose of 40 mg/kg of body weight. After 1 h, the small intestine was removed and used for the measurement of parameters of oxidative stress and mitochondrial and BBM (brush border membrane) function. Evidence of oxidative stress was found in the mucosa of the small intestine of drug-treated rats, as indicated by significantly increased activity of xanthine oxidase (P<0.001) and myeloperoxidase (P<0.001), with corresponding decreases in the levels of several free radical scavenging enzymes and a-tocopherol (P<0.001 in all cases). Levels of products of peroxidation were also significantly elevated (P<0.001 for all the parameters measured). In addition, oxidative stress was evident in isolated intestinal mitochondria and BBMs (P<0.001 for all the parameters measured), with associated alterations in function of these organelles (P<0.001 for all the parameters measured). Supplementation of the diet with glutamine or glutamic acid prior to treatment with indomethacin produced significant amelioration in all the effects produced by the drug in the small intestine (P<0.001 for all the parameters measured). Glycine and alanine were found to be much less effective in these respects

Forest Cover Associated with Improved Child Health and Nutrition: Evidence from the Malawi Demographic and Health Survey and Satellite Data

Healthy forests provide human communities with a host of important ecosystem services, including the provision of food, clean water, fuel, and natural medicines. Yet globally, about 13 million hectares of forests are lost every year, with the biggest losses in Africa and South America. As biodiversity loss and ecosystem degradation due to deforestation continue at unprecedented rates, with concomitant loss of ecosystem services, impacts on human health remain poorly understood. Here, we use data from the 2010 Malawi Demographic and Health Survey, linked with satellite remote sensing data on forest cover, to explore and better understand this relationship. Our analysis finds that forest cover is associated with improved health and nutrition outcomes among children in Malawi. Children living in areas with net forest cover loss between 2000 and 2010 were 19% less likely to have a diverse diet and 29% less likely to consume vitamin A-rich foods than children living in areas with no net change in forest cover. Conversely, children living in communities with higher percentages of forest cover were more likely to consume vitamin A-rich foods and less likely to experience diarrhea. Net gain in forest cover over the 10-year period was associated with a 34% decrease in the odds of children experiencing diarrhea (P5.002). Given that our analysis relied on observational data and that there were potential unknown factors for which we could not account, these preliminary findings demonstrate only associations, not causal relationships, between forest cover and child health and nutrition outcomes. However, the findings raise concerns about the potential short- and long-term impacts of ongoing deforestation and ecosystem degradation on community health in Malawi, and they suggest that preventing forest loss and maintaining the ecosystems services of forests are important factors in improving human health and nutrition outcomes

The Neoliberalization of Higher Education: Paradoxing Students\u27 Basic Needs at a Hispanic-Serving Institution

Millions of college students in the United States lack access to adequate food, housing, and other basic human needs. These insecurities have only been exacerbated in recent decades by the country\u27s neoliberal approach to higher education, with disproportionately negative consequences for historically underserved populations (e.g., racial/ethnic minorities, low-income students, and first-generation college students). For each of these reasons, this study explores the organizational paradoxes faced by students attending a public, 4-year Hispanic-Serving Institution (HSI) in southern California. Drawing upon 30 semi-structured interviews with undergraduates who self-identified as historically underserved, our three-stage conceptualization of data analysis revealed three specific paradoxes: (1) provision vs. dependence, (2) sympathy vs. distancing, and (3) bootstrapping vs. unattainability. We conclude with practical and theoretical implications for alleviating the repercussions of neoliberal policies on today\u27s college students

Expression analysis of rubber biosynthetic pathway genes in Hevea brasiliensis

Hevea brasiliensisis, the primary commercial source of natural rubber (cis-1, 4-polyisoprene), is a fundamental raw material used for manufacturing a wide range of industrial and domestic rubber products in automobile, medical and defense industries. In Hevea, biosynthesis of rubber takes place through mevalonate pathway. Clonal variations in the productivity of rubber may be the result of variations in the activities of the enzymes involved in rubber biosynthesis in different Hevea clones. In this study, expression of 14 genes corresponding to enzymes/regulatory proteins involved in rubber biosynthesis was analyzed in high and low latex yielding clones of Hevea brasiliensis. The level of expression of HbSUT3, a sucrose transporter and enzymes related to the synthesis of rubber such as hydroxymethyl glutaryl-CoA synthase (hmgs), HMG-CoA reductase (hmgr) and mevalonate diphosphate decarboxylase (MVD) were found to be significantly higher in high rubber yielding clones compared to the low rubber yielding clones. The higher expression of these genes might result in an increased supply of IPP, the isoprenoid monomer, required for rubber biosynthesis. Expression of genes in the downstream rubber biosynthetic pathway such as FPPS, RuT and REF2 were also found to be significantly higher in high rubber yielding clones than low yielders. The results suggest that high rubber yield is associated with high expression of these genes and these genes can be used as markers for high yield potential in Hevea

Characterization of a broad-based mosquito yeast interfering RNA larvicide with a conserved target site in mosquito semaphorin-1a genes

BACKGROUND: RNA interference (RNAi), which has facilitated functional characterization of mosquito neural development genes such as the axon guidance regulator semaphorin-1a (sema1a), could one day be applied as a new means of vector control. Saccharomyces cerevisiae (baker's yeast) may represent an effective interfering RNA expression system that could be used directly for delivery of RNA pesticides to mosquito larvae. Here we describe characterization of a yeast larvicide developed through bioengineering of S. cerevisiae to express a short hairpin RNA (shRNA) targeting a conserved site in mosquito sema1a genes. RESULTS: Experiments conducted on Aedes aegypti larvae demonstrated that the yeast larvicide effectively silences sema1a expression, generates severe neural defects, and induces high levels of larval mortality in laboratory, simulated-field, and semi-field experiments. The larvicide was also found to induce high levels of Aedes albopictus, Anopheles gambiae and Culex quinquefasciatus mortality. CONCLUSIONS: The results of these studies indicate that use of yeast interfering RNA larvicides targeting mosquito sema1a genes may represent a new biorational tool for mosquito control

Critical research needs for successful food systems adaptation to climate change

There is a growing sense of the fragility of agricultural production in the Global North and South and of increasing risks to food security, as scientific observations confirm significant changes in the Gulf Stream, polar ice, atmospheric CO2, methane release, and other measures of climate change. This sense is heightened as each of us experiences extreme weather, such as the increasing frequency of droughts, floods, unseasonal temperatures, and erratic seasonality. The central research challenge before us is how global, national, regional, and local food systems may adapt to accelerating climate change stresses and uncertainties to ensure the availability, access, consumption, and stability of healthy food for and by all people. Missing aspects of research fall into two broad categories: the impacts of rapid climate change on the environmental systems supporting food production, and climate change’s impact on the predominantly human systems that influence food security. Of particular concern is how different policy and governance mechanisms can support or hinder the collective decision-making needed to promote a swift adaptive response to increase and sustain food security. Human systems research is needed to investigate food system activities beyond production (processing, distribution, consumption, and waste management). It also must consider political, cultural, and regulatory factors that influence behavior and facilitate positive behavioral changes. To accurately envision future scenarios, research is needed to characterize risk comprehensively throughout the food system, assess barriers to and opportunities for changing food systems, and evaluate novel and traditional approaches that may lead to greater food security

Food webs and food sovereignty: Research agenda for sustainability

Future food production will be constrained by the scarcity of fossil fuel and fresh water as well as increasing intensity and unpredictability of weather events and climate changes. The assurance of food security and equity for many consumers is complicated by concentration of ownership of land and other production resources, as well as a global corporate food systems model that is driven by profit at the expense of people and the environment. To assess potential alternatives to the contemporary global food chain, well focused research is needed on local food production and food webs where small- and midscale family farms provide economic viability for rural communities and their regions. We suggest multiple and integrative research priorities in production, enterprise, and farm economics, environmental impacts of farming at different scales, and social and community consequences of value adding and economic multipliers in local food webs and systems as well as the structure of agriculture. Research into key questions on food security and how it relates to increased food sovereignty is clearly needed to assess creative food system alternatives for the future

Stress-shape misalignment in confluent cell layers

This study investigates the relationship between cell shape and cell-generated stresses in confluent cell layers. Using simultaneous measurements of cell shape orientation and cell-generated contractile forces in MDCK and LP-9 colonies, we report the emergence of correlated, dynamic domains in which misalignment between the directors defined by cell shape and by contractile forces reaches up to 90$^o$, effectively creating extensile domains in a monolayer of contractile cells. To understand this misalignment, we develop a continuum model that decouples the orientation of cell-generated active forces from the orientation of the cell shapes. This challenges the prevailing understanding that cells throughout a tissue create either contractile or extensile forces, and the validity of the usual active nematic models of cell motility where active forces are strictly slaved to cell shape orientation.Comment: 10 pages, 6 figure