Modelling environmental factors correlated with podoconiosis: a geospatial study of non-filarial elephantiasis

Introduction The precise trigger of podoconiosis — endemic non-filarial elephantiasis of the lower legs — is unknown. Epidemiological and ecological studies have linked the disease with barefoot exposure to red clay soils of volcanic origin. Histopathology investigations have demonstrated that silicon, aluminium, magnesium and iron are present in the lower limb lymph node macrophages of both patients and non-patients living barefoot on these clays. We studied the spatial variation (variations across an area) in podoconiosis prevalence and the associated environmental factors with a goal to better understanding the pathogenesis of podoconiosis. Methods Fieldwork was conducted from June 2011 to February 2013 in 12 kebeles (administrative units) in northern Ethiopia. Geo-located prevalence data and soil samples were collected and analysed along with secondary geological, topographic, meteorological and elevation data. Soil data were analysed for chemical composition, mineralogy and particle size, and were interpolated to provide spatially continuous information. Exploratory, spatial, univariate and multivariate regression analyses of podoconiosis prevalence were conducted in relation to primary (soil) and secondary (elevation, precipitation, and geology) covariates. Results Podoconiosis distribution showed spatial correlation with variation in elevation and precipitation. Exploratory analysis identified that phyllosilicate minerals, particularly clay (smectite and kaolinite) and mica groups, quartz (crystalline silica), iron oxide, and zirconium were associated with podoconiosis prevalence. The final multivariate model showed that the quantities of smectite (RR = 2.76, 95% CI: 1.35, 5.73; p = 0.007), quartz (RR = 1.16, 95% CI: 1.06, 1.26; p = 0.001) and mica (RR = 1.09, 95% CI: 1.05, 1.13; p < 0.001) in the soil had positive associations with podoconiosis prevalence. Conclusions More quantities of smectite, mica and quartz within the soil were associated with podoconiosis prevalence. Together with previous work indicating that these minerals may influence water absorption, potentiate infection and be toxic to human cells, the present findings suggest that these particles may play a role in the pathogenesis of podoconiosis and acute adenolymphangitis, a common cause of morbidity in podoconiosis patients

Use of heavy metals in swine feed and its association with the co-selection of metal tolerant and multi-drug resistant Salmonella

This study was conducted to characterize the role of chemical interventions, biocides and heavy metal micronutrients, in particular, in swine production systems on the emergence of heavy metal and biocide tolerant Salmonella and its association with antimicrobial resistance. A total of 353 Salmonella isolates with different antimicrobial resistance profiles identified from 36 barns exposed to three different classes of biocides were analyzed. The sources of isolates included feed (n=30), fecal (n=226), and environment (n=97) samples that were systematically selected

E-readiness to G-readiness: developing a green information technology readiness framework

Businesses are under increasing pressure from competitors, regulators and community groups to implement sustainable business practices. Balancing economic and environmental performance to be green and competitive is therefore a key strategic issue. The increased discussion on green information technology (IT) has sparked the interest of this research. Green IT is poised to influence not only technology but also competitive strategy and even the legality of some business strategic options. Understanding and leveraging Green IT is therefore critical for businesses¿ continued progress. Nevertheless, the principles, practices and value of Green IT is yet to be researched. This paper introduces the concept of Green IT and describes the main pillars of a g-readiness framework to help organisations evaluate their readiness for adopting Green IT. It argues that just as e-readiness has been, and continues to be, a critical quality in the digital economy, g-readiness is an equally critical quality in the low carbon digital economy. Without a clear understanding of g-readiness, organisations would approach Green IT initiatives on an ad hoc and somewhat reactive basis which is undesirable

Glyphosate resistance by engineering the flavoenzyme glycine oxidase.

Glycine oxidase from Bacillus subtilis is a homotetrameric flavoprotein of great potential biotechnological use because it catalyzes the oxidative deamination of various amines and D-isomer of amino acids to yield the corresponding \u3b1-keto acids, ammonia/amine, and hydrogen peroxide. Glyphosate (N-phosphonomethylglycine), a broad spectrum herbicide, is an interesting synthetic amino acid: this compound inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, which is essential for the biosynthesis of aromatic amino acids in plants and certain bacteria. In recent years, transgenic crops resistant to glyphosate were mainly generated by overproducing the plant enzyme or by introducing a 5-enolpyruvylshikimate-3-phosphate synthase insensitive to this herbicide. In this work, we propose that the enzymatic oxidation of glyphosate could be an effective alternative to this important biotechnological process. To reach this goal, we used a rational design approach (together with site saturation mutagenesis) to generate a glycine oxidase variant more active on glyphosate than on the physiological substrate glycine. The glycine oxidase containing three point mutations (G51S/A54R/H244A) reaches an up to a 210-fold increase in catalytic efficiency and a 15,000-fold increase in the specificity constant (the kcat/Km ratio between glyphosate and glycine) as compared with wild-type glycine oxidase. The inspection of its three-dimensional structure shows that the \u3b12-\u3b13 loop (comprising residues 50-60 and containing two of the mutated residues) assumes a novel conformation and that the newly introduced residue Arg54 could be the key residue in stabilizing glyphosate binding and destabilizing glycine positioning in the binding site, thus increasing efficiency on the herbicide

Modern contraceptive use and associated factors during extended postpartum period among women who gave birth in the last 12 months at Northwest Ethiopia

Background: The extended postpartum period is a one-year follow-up period after giving birth, and it is critical for women to prevent unintended pregnancy and reduce the risk of maternal and child mortality by ensuring safe birth intervals. Many women, however, are unaware that they are at risk for pregnancy throughout this period. Hence, the aim of this study was to assess the utilization and associated factors of modern contraceptives during extended postpartum family planning (EPPP) in northwest Ethiopia. Methods: A community-based cross-sectional study design was conducted using 630 samples from October 01 to October 30, 2020, in northwest Ethiopia. The study participants were drawn through a multistage sampling technique and data were collected using structured questionnaires via interview. The collected data were entered into EpiData version 4.2 and exported into SPSS version 25.0 for management and further analysis. A bivariable logistic regression model was used to identify variables having an association with the outcome variable. In bivariable analysis, variables having P ≤ 0.25 were selected and entered into multivariable logistic regression analysis. Finally, in multivariable analysis, variables having P ≤ 0.05 with a 95% CI were declared as significantly associated with the outcome variable. Results: About 60.6% of women were using modern contraceptive during extended postpartum period. Mothers to partner discussion (AOR= 7.6, 95% CI: 4.20– 14.05), secondary educational status (AOR= 3.8, 95% CI: 1.36– 10.93), college and above educational status (AOR= 7, 95% CI: 1.92– 25.57), menstrual resumption (AOR= 9.2, 95% CI: 5.66– 15.12), sex resumed (AOR=8.5, 95% CI: 2.19– 33.58), fertility desire (AOR= 3.9, 95% CI: 1.99– 6.15), linkage to FP during child immunization (AOR= 2.7, 95% CI: 1.67– 4.50), and FP counseling during pregnancy (AOR=2, 95% CI: 1.25– 3.34) were significantly associated with outcome variable. Conclusion: Associating factors were identified as partner discussion, education, menstrual resumption, fertility desire, sexual resumption, FP counseling, and FP during child immunization. Improving mothers’ education and informing couples about the dangers of becoming pregnant before menstruation are critical

ORIGINAL COMMUNICATION Effects on health of fluid restriction during fasting in Ramadan

During the 9th month (Ramadan) of the Islamic calendar (Hijra) many millions of adult Muslims all over the world fast during the daylight hours. Since Hijra is a lunar calendar, Ramadan occurs at different times in the seasonal year over a 33-year cycle. Fasting during Ramadan is partial because the abstention from food, fluid, tobacco and caffeine is from sunrise to sunset. Several categories of people are exempt or can postpone the Ramadan fast. The effect on health and well being of the month-long intermittent fast and fluid restriction has been studied in various potentially vulnerable groups in addition to normal healthy individuals in many countries. The majority of the studies have found significant metabolic changes, but few health problems arising from the fast. A reduction in drug compliance was an inherent negative aspect of the fast. Common findings of the studies reviewed were increased irritability and incidences of headaches with sleep deprivation and lassitude prevalent. A small body mass loss is a frequent, but not universal, outcome of Ramadan. During the daylight hours of Ramadan fasting, practising Muslims are undoubtedly dehydrating, but it is not clear whether they are chronically hypohydrated during the month of Ramadan. No detrimental effects on health have as yet been directly attributed to negative water balance at the levels that may be produced during Ramadan

Implementasi Algoritma K-Means untuk Mengenali Pola Jemaat dalam Kegiatan Pelayanan Gereja

Gereja adalah sebuah institusi yang tidak hanya mengelola kerohanian jemaatnya, namun juga merupakan wadah organisasi yang mengayomi dan melindungi jemaat serta menjamin keberlangsungan kehidupan sosial masyarakat jemaatnya. Data statistik juga menunjukkan jumlah pemeluk agama Kristen Protestan di kota Kupang mencapai angka 209.438 orang atau sebanyak 61,08% dari jumlah total penduduk di kota Kupang. Jumlah gereja Kristen Protestan sebanyak 150 gereja sehingga diasumsikan setiap gereja memiliki jemaat rata-rata 1.396 jemaat per gereja. &nbsp;Data mining&nbsp;merupakan proses yang mempekerjakan satu atau lebih teknik pembelajaran komputer (machine learning) untuk menganalisis dan mengekstrasi pengetahuan sehingga didapat pola tertentu. Penelitian ini akan mengimplementasikan algoritma data mining K-Means Clustering untuk mengenali pola jemaat yang menjadi salah satu target kegiatan pelayanan gereja yaitu jemaat yang belum dibaptis padahal sudah melewati usia baptis pada umumnya sebanyak . Luaran utama yaitu berupa rumusan/pola jemaat dan hasil rumusan itu akan dipakai oleh pihak gereja untuk melakukan pendekatan kepada jemaat dengan ciri/pola tersebut

The stellar populations of spiral disks.II Measuring and modeling the radial distribution of absorption spectral indices

The radial distributions of the Mg2 and Fe5270 Lick spectral indices have been measured to large radial distances on the disks of NGC 4303 and NGC 4535 using an imaging technique based on interference filters. These data, added to those of NGC 4321 previously published in Paper I of this series are used to constraint chemical (multiphase) evolutionary models for these galaxies. Because the integrated light of a stellar disk is a time average over the history of the galaxy weighted by the star formation rate, these constraints complement the information on chemical gradients provided by the study of HII regions which, by themselves, can only provide the alpha-elements abundance accumulate over the life of the galaxy. The agreement between the observations and the model predictions shown here lends confidence to the models which are then used to describe the time evolution of galaxy parameters such as star formation rates, chemical gradients, and gradients in the mean age of the stellar population.Comment: to be published in Astrophysical Journa

Modeling the radial abundance distribution of the transition galaxy ngc 1313

NGC 1313 is the most massive disk galaxy showing a flat radial abundance distribution in its interstellar gas, a behavior generally observed in magellanic and irregular galaxies. We have attempted to reproduce this flat abundance distribution using a multiphase chemical evolution model, which has been previously used sucessfully to depict other spiral galaxies along the Hubble morphological sequence. We found that it is not possible to reproduce the flat radial abundance distribution in NGC 1313, and at the same time, be consistent with observed radial distributions of other key parameters such the surface gas density and star formation profiles. We conclude that a more complicated galactic evolution model including radial flows, and possibly mass loss due to supernova explosions and winds, is necessary to explain the apparent chemical uniformity of the disk of NGC 1313Comment: 14 paginas, 4 figures, to be published in ApJ, apri