Estandarización de procesos en la gestión de proyectos del departamento de Planta Física de la Universidad EAFIT basados en la metodología Prince 2, PMI e integrada a los procesos BIM

La metodología de trabajo por proyectos es uno de los esquemas más comunes como las empresas u organizaciones asumen sus nuevos desarrollos o inversiones; el área de Planta Física de la Universidad EAFIT no es ajena a dicho esquema y es así como ha asumido el desarrollo de todos los proyectos de crecimiento de infraestructura tanto en instalaciones técnicas, nuevas construcciones o actualizaciones a las mismas. El presente trabajo hace una revisión por varias metodologías de proyectos como son PMI (Project Management Institute); PRINCE 2 (PRojects IN Controlled Environment), CP3M (Modelo de Madurez – grupo de investigación GYEPRO) para establecer una metodología al interior del área a partir de identificación del grado de madurez del equipo de trabajo y de esta manera permitir trazar los planes y componentes requeridos para el desarrollo de cualquier proyecto que se desarrolle dentro del área de acuerdo con una clasificación previa que se indique para el proyecto. Además, se definen los componentes que podrían incluirse en la PWA que facilitaría la planificación, seguimiento y control de los distintos proyectos que se desarrollan en el área de Planta física

A theoretical study on the simultaneous vapor-liquid and chemical equilibria in a highly restricted system

In this work, the isomerization reaction of n-butane (C4(2)) to isobutane (iC4(1)) in the absence of other components is studied with the help of a model, in a relatively wide pressure range, both, under single fluid phase conditions and under vapor-liquid equilibrium conditions. In this last case the phase and chemical equilibrium are solved simultaneously. This binary system was chosen, among other considerations, because of the low number of degrees of freedom that it has, according to the phase rule for reactive systems. Such high level of restriction leads to a peculiar behavior that provides interesting insights, in particular on the simultaneous chemical and phase equilibrium. The C4(2) + iC4(1) system is represented in this work by the Soave-Redlich-Kwong equation of state coupled to quadratic mixing rules with binary interaction parameters set to zero. The required computation algorithms were developed in this work. The computed fluid phase equilibrium of this reactive system is a single univariant vapor-liquid equilibrium line. Such line ends at the only reactive vapor-liquid critical point that the system has. Some reactive isochores (or isotherms) were also computed. The obtained computation results show that for this system the conversion can be changed, under certain conditions, just by modifying the overall density, while keeping the temperature and the pressure at constant values.Fil: Molina, Matías José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Rodriguez Reartes, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Zabaloy, Marcelo Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentin

Baculovirus vectors induce the production of interferons in swine: Their potential in the development of antiviral strategies

The huge variety of viruses affecting swine represents a global threat. Since vaccines against highly contagious viruses last several days to induce protective immune responses, antiviral strategies for rapid control of outbreak situations are needed. The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), an insect virus, has been demonstrated to be an effective vaccine vector for mammals. Besides the ability to display or transduce heterologous antigens, it also induces strong innate immune responses and provides IFN-mediated protection against lethal challenges with viruses like foot-and-mouth disease virus (FMDV) in mice. Thus, the aim of this study was to evaluate the ability of AcMNPV to induce IFN production and elicit antiviral activity in porcine peripheral blood mononuclear cells (PBMCs). Our results demonstrated that AcMNPV induced an IFN-α-mediated antiviral activity in PBMCs in vitro. Moreover, the inoculation of AcMNPV in piglets led to the production of type I and II IFNs in sera from inoculated animals and antiviral activities against vesicular stomatitis virus (VSV) and FMDV measured by in vitro assays. Finally, it was demonstrated that the pseudotyping of AcMNPV with VSV-G protein, but not the enrichment of the AcMNPV genome with specific immunostimulatory CpG motifs for the porcine TLR9, improved the ability to induce IFN-α production in PBMCs in vitro. Together, these results suggest that AcMNPV is a promising tool for the induction of IFNs in antiviral strategies, with the potential to be biotechnologically improved.Fil: Molina, Guido Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Amalfi, Sabrina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Otero, Ignacio. Universidad Maimónides; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentin

Metabolomics Analysis Reveals Altered Metabolic Pathways and Response to Doxorubicin in Drug-Resistant Triple-Negative Breast Cancer Cells

This study aimed to investigate metabolic changes following the acquisition of resistance to doxorubicin in the triple-negative breast cancer (TNBC) cell line MDA-MB-231. Two drug-resistant cell lines, DOX-RES-50 and DOX-RES-100, were generated by treating MDA-MB-231 cells with doxorubicin for 24 h and allowing them to recover for six weeks. Both drug-resistant cell lines demonstrated an increase in doxorubicin IC50 values, indicating acquired drug resistance. Metabolomics analysis showed clear separation between the parental MDA-MB-231 cell line and the drug-resistant cell lines. Pathway analysis revealed that arginine and proline metabolism, glutathione metabolism, and beta-alanine metabolism were significantly perturbed in the drug-resistant cell lines compared to the parental cell line. After matching signals to an in-house library of reference standards, significant decreases in short- and medium-chain acylcarnitines and significant increases in long-chain acylcarnitines, 5-oxoproline, and 7-ketodeoxycholic acid were observed in the resistant cell lines as compared to the parental MDA-MB-231 cell line. In addition to baseline metabolic differences, we also investigated differences in metabolic responses in resistant cell lines upon a second exposure at multiple concentrations. Results indicate that whereas the parental MDA-MB-231 cell line had many metabolites that responded to doxorubicin in a dose-dependent manner, the two resistant cell lines lost a dose-dependent response for the majority of these metabolites. The study’s findings provide insight into how metabolism is altered during the acquisition of resistance in TNBC cells and how the metabolic response to doxorubicin changes upon repeated treatment. This information can potentially identify novel targets to prevent or reverse multi-drug resistance in TNBC, and also demonstrate the usefulness of metabolomics technology in identifying new mechanisms of drug resistance in cancer and potential drug targets

Baculovirus Transduction in Mammalian Cells Is Affected by the Production of Type I and III Interferons, Which Is Mediated Mainly by the cGAS-STING Pathway

The baculovirus Autographa californica multiple nucleopolyhedrovirus is an insect virus with a circular double-stranded DNA genome, which, among other multiple biotechnological applications, is used as an expression vector for gene delivery in mammalian cells. Nevertheless, the nonspecific immune response triggered by viral vectors often suppresses transgene expression. To understand the mechanisms involved in that response, in the present study, we studied the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway by using two approaches: the genetic edition through CRISPR/Cas9 technology of genes encoding STING or cGAS in NIH/3T3 murine fibroblasts and the infection of HEK293 and HEK293 T human epithelial cells, deficient in cGAS and in cGAS and STING expression, respectively. Overall, our results suggest the existence of two different pathways involved in the establishment of the antiviral response, both dependent on STING expression. Particularly, the cGAS-STING pathway resulted in the more relevant production of beta interferon (IFN-β) and IFN-λ1 in response to baculovirus infection. In human epithelial cells, IFN-λ1 production was also induced in a cGAS-independent and DNA-protein kinase (DNA-PK)-dependent manner. Finally, we demonstrated that these cellular responses toward baculovirus infection affect the efficiency of transduction of baculovirus vectors. IMPORTANCE Baculoviruses are nonpathogenic viruses that infect mammals, which, among other applications, are used as vehicles for gene delivery. Here, we demonstrated that the cytosolic DNA sensor cGAS recognizes baculoviral DNA and that the cGAS-STING axis is primarily responsible for the attenuation of transduction in human and mouse cell lines through type I and type III IFNs. Furthermore, we identified DNA-dependent protein kinase (DNA-PK) as a cGAS-independent and alternative DNA cytosolic sensor that contributes less to the antiviral state in baculovirus infection in human epithelial cells than cGAS. Knowledge of the pathways involved in the response of mammalian cells to baculovirus infection will improve the use of this vector as a tool for gene therapy.Fil: Amalfi, Sabrina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Molina, Guido Nicolás. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Bevacqua, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: López, María Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentin

Composição corporal de uma amostra de mulheres mexicanas e sua relação com o IMC / Body composition of a sample of mexican women and its relationship with BMI

A obesidade é considerada um problema de saúde pública e a realidade do México não é diferente, já que sua prevalência foi estimada em 31%.O objetivo deste trabalho foi descrever a composição corporal de uma mostra de mexicanas residentes em Xalapa, Vera Cruz e fazer uma comparação como IMC. Foram avaliadas de maneira voluntaria e com o consseentimento informado, e aprovação da Secretaria e Direção de Serviços de Saúde de Vera Cruz, 18 mulheres mediante a aplicação do método de Fracionamento Antropométrico (ENFA®). As 25 medidas de superfície foram realizadas por nutricionista especializada e refente em antropometria. Determinaram-se as cinco massas corporais: Massa Esquelética, Massa Lipídica, Massa Muscular, Massa Visceral e Massa Residual. Foram calculadas as reservas corporais: Reserva Protéica (RP= massa muscular/ massa esquelética) e Reserva Calórica (RC=massa lipídica/ massa esquelética). A população de referência utilizada foi o Phantom.A média de idade encontrada foi de 25.82 anos ± 3.91. A altura média de 157.8cm ±3.00 foi significativamente menor que o Phantom (teste T=-17.53 e αalpha‹0.000) e a altura sentada 86.4cm ± 3.22 também (teste T=-4.65 e αalpha;‹0.000). No entanto, o diâmetro Anteroposterior do tórax 20.97cm ± 1.42 foi significativamente maior que o Phantom (teste T =10.38 e αalpha‹0.000). A média do IMC foi de 28.21. A Reserva Lipídica encontrada de 1.55 ± 0.31 (VN=0.85 a 1.47) e a Reserva Protéica 2.35 ± 0.29 (VN=2.15 a 2.72). Somente 9 mulheres apresentaram Reserva Lipídica aumentada, no entanto o IMC encontrado foi elevado em todas as mulheres. A Reserva Lipídica normal é explicada pelo tamanho grande do esqueleto que possuem: 9.2 kg ± 0.45.  Em função ao tamanho do esqueleto precisa-se de uma determinada quantidade de massa muscular e lipídica. Isso explicaria a diferença encontrada em ambos métodos

Algoritmos de cálculo de envolventes de fases de sistemas reactivos multicomponente

Una envolvente de fases reactiva (EFR) es la frontera, en el plano presión-temperatura, que separa la región de homogeneidad de la de heterogeneidad, para un dado sistema multicomponente reactivo de composición global inicial especificada. En un punto de la EFR se satisfacen la igualdad de potenciales químicos, la condición de equilibrio químico para cada una de las reacciones químicas posibles, la conservación de la masa y la incipiencia de una de las fases. El propósito del presente trabajo es el desarrollo de algoritmos para el cálculo de EFRs. Una vez obtenido un punto convergido de la EFR, se aplica un método de continuación numérica capaz de obtener un altamente no lineal set continuo de soluciones del sistema de ecuaciones apropiado, el cual corresponde a un punto de saturación reactivo.Fil: Molina, Matías José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química; ArgentinaFil: Rodriguez Reartes, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química; ArgentinaFil: Zabaloy, Marcelo Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina. Universidad Nacional del Sur. Departamento de Ingeniería Química; ArgentinaXXII Congreso Argentino de Fisicoquímica y Química InorgánicaLa PlataArgentinaUniversidad Nacional de la Plata, Facultad de IngenieríaAsociación Argentina de Investigaciones Fisicoquímica

Metabolomics Analysis Reveals Novel Targets of Chemosensitizing Polyphenols and Omega-3 Polyunsaturated Fatty Acids in Triple Negative Breast Cancer Cells

Triple negative breast cancer (TNBC) is a subtype of breast cancer with typically poorer outcomes due to its aggressive clinical behavior and lack of targeted treatment options. Currently, treatment is limited to the administration of high-dose chemotherapeutics, which results in significant toxicities and drug resistance. As such, there is a need to de-escalate chemotherapeutic doses in TNBC while also retaining/improving treatment efficacy. Dietary polyphenols and omega-3 polyunsaturated fatty acids (PUFAs) have been demonstrated to have unique properties in experimental models of TNBC, improving the efficacy of doxorubicin and reversing multi-drug resistance. However, the pleiotropic nature of these compounds has caused their mechanisms to remain elusive, preventing the development of more potent mimetics to take advantage of their properties. Using untargeted metabolomics, we identify a diverse set of metabolites/metabolic pathways that are targeted by these compounds following treatment in MDA-MB-231 cells. Furthermore, we demonstrate that these chemosensitizers do not all target the same metabolic processes, but rather organize into distinct clusters based on similarities among metabolic targets. Common themes in metabolic targets included amino acid metabolism (particularly one-carbon and glutamine metabolism) and alterations in fatty acid oxidation. Moreover, doxorubicin treatment alone generally targeted different metabolites/pathways than chemosensitizers. This information provides novel insights into chemosensitization mechanisms in TNBC

Commonalities in Metabolic Reprogramming between Tobacco Use and Oral Cancer

Tobacco use is a major public health concern and is linked to myriad diseases, including cancer. The link between tobacco use and oral cancer, specifically, is very strong, making tobacco use one of the primary risk factors for oral cancer. While this association is well known, the underlying biochemical changes that result from tobacco use, and how this links to metabolic phenotypes of oral cancer, is not well understood. To address this knowledge gap, a combination of literature reviews and metabolomics studies were performed to identify commonalities in metabolic perturbations between tobacco use and oral cancers. Metabolomics analysis was performed on pooled reference urine from smokers and non-smokers, healthy and malignant oral tissues, and cultured oral cells with or without treatment of the well-known tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Alterations in amino acid metabolism, carbohydrates/oxidative phosphorylation, fatty acid oxidation, nucleotide metabolism, steroid metabolism, and vitamin metabolism were found to be shared between tobacco use and oral cancer. These results support the conclusion that tobacco use metabolically reprograms oral cells to support malignant transformation through these pathways. These metabolic reprogramming events may be potential targets to prevent or treat oral cancers that arise from tobacco use