The effect of thyroxine on percentage of hatching (Hypophthalmichthys molitrix)

Studies showed that Thyroxine can play an important role on fish growth, osmoregulation system, and other physiological activities. Since the direct effect of thyroxine on growth and metamorphose of bony fish larvae has not been yet approved, therefore, present study were carried out in Shahid Ansary propagation and cultivation farm in order to find out the way of effect of this hormone on the survirval rate and early growth stage of silver carp (Hypophthalmichthys molitrix) fertiled eggs that were bathed in thyroxin with concentrations of 0.1, 0.5 and 1.0 ppm (each one, replicates) for 6 hours. Then they were transfered into large incubators (zoog) with running water. After hatching all larvae were placed in mentioned thyroxin concentration bath for another 6 hours before being transfered into large incubators. Larvae stayed inzoogs until the yolk-sac was absorbed. Finally, live silver carp larvae were counted in separated plates. Results of present study indicated that the ra tio of hatched eggs in 0.5 ppm of thyroxin concentration was higher than other treatment

Intranasal administration of endometrial mesenchymal stem cells as a suitable approach for Parkinson�s disease therapy

This study aimed to investigate the therapeutic effects of intranasal administration of human endometrium-derived stem cells (HEDSCs) in the mouse model of Parkinson�s disease (PD). Thirty days after intrastriatal injection of 6-OHDA, HEDSCs were administrated intranasally in three doses (104, 5 � 104 and 105 cells µl�1). During 120 days after stem cell administration, behavioral tests were examined. Then the mice were sacrificed and the fresh section of the substantia nigra pars compacta (SNpc) was used for detection of HEDSCs-GFP labeled by fluorescence microscopy method. In addition, immunohistochemistry was used to assay GFP, human neural Nestin, and tyrosine hydroxylase (TH) markers in the fixed brain tissue at the SNpc. Our data revealed that behavioral parameters were significantly improved after cell therapy. Fluorescence microscopy assay in fresh tissue and GFP analysis in fixed tissue were showed that the HEDSCs-GFP labeled migrated to SNpc. The data from immunohistochemistry revealed that the Nestin as a differential neuronal biomarker was expressed in SNpc. Also, TH as a dopaminergic neuron marker significantly increased after HEDSCs therapy in an optimized dose 5 � 104 cells µl�1. Our results suggest that intranasal administration of HEDSCs improve the PD symptoms in the mouse model of PD dose-dependent manner as a noninvasive method. © 2019, Springer Nature B.V

Cultural Aspects of Social Anxiety Disorder: A Qualitative Analysis of Anxiety Experiences and Interpretation

Objective: Anxiety is a complex phenomenon on which culture has a prominent influence. The present study aimed to investigate the cultural aspects of social anxiety disorder (SAD) in an Iranian population. Method: A qualitative content analysis research was done to answer the study question. A total of 16 individuals with social anxiety disorder (six men and 10 women) were selected using purposeful sampling method (M = 24.43, SD = 4.56). The study was conducted in Tehran, Urmia, and Sanandaj- Iran. Participants were from different ethnic backgrounds (LOR, FARS, TURK, and KURD). Data were analyzed by thematic analysis using an inductive method. Results: Analysis of participants’ records yielded five distinct categories with some subcategories, which are as follow: (1) anxiety experiences; (2) core beliefs; (3) reasons of being anxious; (4) effects of SAD on life aspects; and (5) coping strategies. Conclusion: It seems that symptoms of social anxiety and its underlying beliefs, causes and effects and coping strategies are almost experienced and interpreted in a way that could be the same as DSM-5 clinical presentation of social anxiety, with the exception that somatic symptoms are experienced by almost all participants

Radiobiological effects of wound fluid on breast cancer cell lines and human-derived tumor spheroids in 2D and microfluidic culture

Intraoperative radiotherapy (IORT) could abrogate cancer recurrences, but the underlying mechanisms are unclear. To clarify the effects of IORT-induced wound fluid on tumor progression, we treated breast cancer cell lines and human-derived tumor spheroids in 2D and microfluidic cell culture systems, respectively. The viability, migration, and invasion of the cells under treatment of IORT-induced wound fluid (WF-RT) and the cells under surgery-induced wound fluid (WF) were compared. Our findings showed that cell viability was increased in spheroids under both WF treatments, whereas viability of the cell lines depended on the type of cells and incubation times. Both WFs significantly increased sub-G1 and arrested the cells in G0/G1 phases associated with increased P16 and P21 expression levels. The expression level of Caspase 3 in both cell culture systems and for both WF-treated groups was significantly increased. Furthermore, our results revealed that although the migration was increased in both systems of WF-treated cells compared to cell culture media-treated cells, E-cadherin expression was significantly increased only in the WF-RT group. In conclusion, WF-RT could not effectively inhibit tumor progression in an ex vivo tumor-on-chip model. Moreover, our data suggest that a microfluidic system could be a suitable 3D system to mimic in vivo tumor conditions than 2D cell culture

Evaluation of NPP1 as a novel biomarker of coronary artery disease: A pilot study in human beings

Purpose: Coronary artery calcification (CAC) is utilized as an important tool for global risk assessment of cardiovascular events in individuals with intermediate risk. Ecto phosphodiesterase/nucleotide phosphohydrolase-1(ENPP1) converts extracellular nucleotides into inorganic pyrophosphate and it is a key regulator of tissue calcification that adjusts calcification in tissues like vascular smooth muscle cells. The main purpose of this clinical study was to find out the correlation between ENPP1 serum concentration and CAC in human for the first time. Methods: In this study 83 patients (16 diabetic patients and 67 non-diabetic patients) with coronary artery disease who fulfilled inclusion and exclusion criteria, entered the study. For all patients a questionnaire consisting demographic data and traditional cardiovascular risk factors were completed. Computed tomography (CT)-Angiography was carried out to determine coronary artery calcium score and enzyme-linked immunosorbent assay (ELISA) method was used for measuring ENPP1 serum concentrations. Results: There was a reverse significant correlation between ENPP1 serum concentration and total CAC score and also CAC of right coronary artery (RCA) (P < 0.05) in non-diabetic patients. Conclusion: On the basis of our results, ENPP1 serum concentration may be a suitable biomarker for coronary artery disease at least in non-diabetic patients. However, more studies with higher sample size are necessary for its confirmation. © 2018 The Authors

Investigating the level of knowledge and performance of postmenopausal women about breast self-examination in Khorramabad in 2019

Background: Due to usually late diagnoses, breast cancer is accompanied by a high mortality rate. Breast self-examination increases the success of treatment and survival rates. This study aimed to evaluate the knowledge and practice of postmenopausal women residing in Khorramabad, Iran, about breast self-examination. Materials and Methods: The present descriptive-analytical study was performed on 333 postmenopausal women referring to health centers in Khorramabad in 2019. The participants were selected using cluster sampling. The required data were collected using a questionnaire containing items on demographic characteristics as well as variables of knowledge and practice of breast self-examination. Data analysis was performed in SPSS software version 22 using the Spearman correlation coefficient, the Chi-square test, and Fisher's exact test. Results: The mean age of the subjects was 55.13±8.13. It was found that 55% of women had moderate knowledge and 43.8% had a moderate performance. The most common source of information was through health center staff (52.9%). Knowledge and performance showed a direct and significant correlation with breast self-examination (r=0.530, P<0.001). There was a statistically significant relationship between knowledge and performance on the one hand, and visiting a doctor and having a history of attending classes on the other (P<0.05). There was also a statistically significant relationship between performance and marital status (P<0.05). Conclusion: Due to the significant relationship between knowledge and performance variables and behaviors such as attending classes and visiting doctors, it is recommended to design educational programs to encourage postmenopausal women to perform breast self-examination