Evaluation of Antileishmanial Activity of Albaha Medicinal Plants against Leishmania amazonensis

Sixteen methanolic extracts obtained from thirteen plant species, selected either from ethnobotanical or chemotaxonomical data, were screened for their antileishmanial activity against Leishmania amazonensis. The cytotoxic activity against normal peritoneal macrophages from normal BALB/c mice was also determined. Eight extracts had IC50 values ranging from <12.5 to 37.8 µg/mL against promastigotes. Achillea biebersteinii flower, Euphorbia helioscopia, and Solanum incanum leaf extracts showed antileishmanial activities with IC50 between <12.5–26.9 µg/mL and acceptable selectivity indices of 8–5. The other leishmanicidal plant extracts, with IC50 ranging from 18.0 to 29.5 µg/mL, exhibited low selectivity indices

Hierarchical cluster analysis and chemical characterisation of <i>Myrtus communis</i> L. essential oil from Yemen region and its antimicrobial, antioxidant and anti-colorectal adenocarcinoma properties

<p>The hydrodistilled essential oil obtained from the dried leaves of <i>Myrtus communis</i>, collected in Yemen, was analysed by GC–MS. Forty-one compounds were identified, representing 96.3% of the total oil. The major constituents of essential oil were oxygenated monoterpenoids (87.1%), linalool (29.1%), 1,8-cineole (18.4%), α-terpineol (10.8%), geraniol (7.3%) and linalyl acetate (7.4%). The essential oil was assessed for its antimicrobial activity using a disc diffusion assay and resulted in moderate to potent antibacterial and antifungal activities targeting mainly <i>Bacillus subtilis, Staphylococcus aureus</i> and <i>Candida albicans.</i> The oil moderately reduced the diphenylpicrylhydrazyl radical (IC₅₀ = 4.2 μL/mL or 4.1 mg/mL). <i>In vitro</i> cytotoxicity evaluation against HT29 (human colonic adenocarcinoma cells) showed that the essential oil exhibited a moderate antitumor effect with IC₅₀ of 110 ± 4 μg/mL. Hierarchical cluster analysis of <i>M. communis</i> has been carried out based on the chemical compositions of 99 samples reported in the literature, including Yemeni sample.</p