43 research outputs found
A highly osmotolerant rhizobial strain confers a better tolerance of nitrogen fixation and enhances protective activities to nodules of Phaseolus vulgaris under drought stress
The effect of water deficiency on nodules of common bean (Phaseolus vulgaris) inoculated with three rhizobial strains differing in their osmotolerance, was investigated in two different experiments on sterile sand. In the first experiment, the control plants were maintained at 90% field capacity (FC) and water-deficient plants were grown at 35% FC. The nitrogen fixation and growth parameters drastically decreased under water deficiency, however the three rhizobial strains, Rhizobium etli A32 (sensitive), Rhizobium tropici CIAT899 (tolerant), and Ensifer meliloti 4H41 (highly tolerant), showed different symbiotic performances. E. meliloti 4H41 allowed the best acetylene reduction activity (ARA) and biomass production and the highest number of large-sized nodules, while no significant effect was observed on lipid peroxidation, protein and legheamoglobin contents. The effect on antioxidant activities was the lowest. In the second experiment, plants were maintained at 90% FC during 45 days and then watering was stopped. The results showed that, the response to water deficit was quite similar for the three analyzed symbioses until 35% FC, but below this value of FC, symbiosis involving strain E. meliloti 4H41 was the most tolerant. This tolerance was accompanied, by in both experiments, by a stability of metabolic indices and protective antioxidant activities. These results suggest that, the relative tolerance of the nodules induced by strain 4H41 could be due to a constructive adaptation involving specific cortex structure and stress-adapted metabolic activities acquired during nodule formation and growth, rather than to a timely inducible response due to the stimulation of antioxidant enzymes. This suggestion should be confirmed through microscopic structure analysis and supplemental key enzymes in nodule metabolism such as sucrose synthase and malate dehydrogenase.Key words: Antioxidant activities, in pots experiment, leghemoglobin content, nodule, rhizobia, osmotolerance, symbiotic efficiency, water deficiency
Manifestations Ophtalmologiques Au Cours Du Syndrome D\'apert : A Propos D\'un Cas
Introduction : Parmi les crâniosténoses, le syndrome d\'Apert demande la collaboration de plusieurs spécialistes, pour sauver ce qui peut l\'être de la fonction visuelle des patients et permettre un développement cérébral le plus proche de la normale.
Observation : Nous présentons le cas d\'une jeune suivie et traitée depuis son jeune âge pour un syndrome d\'Apert. Elle a subi plusieurs interventions successives pour garder à un âge assez avancé une fonction visuelle appréciable à 3/10.Les modifications anatomiques ont inéluctablement influé sur l\'état visuel de la patiente avec une myopie forte,un
astigmatisme relativement important et une atrophie papillaire partielle. Discussion : D\'origine génétique, le syndrome d\'Apert est dû à une mutation allélique du récepteur 2 d\'un facteur fibroblastique. Les signes de souffrance cérébrale sont inévitables, et l\'atrophie optique relative représente la séquelle fonctionnelle principale. Conclusion : Une prise en charge de longue haleine est nécessaire dans le syndrome d\'Apert pour espérer sauver une
fonction visuelle utile.Introduction : Several craniosynostotic syndromes are described such as Apert syndrome in which collaboration between different specialists is necessary to preserve visual function and to allow normal cerebral development. Case-report : It\'s a case note of a girl with Apert syndrome. She underwent since her infancy several surgical operations. Anatomic modifications affected her visual status with a best visual acuity of 3/1O, high myopia, astigmatism and partial optic atrophy. Discussion : Apert syndrome is a genetic disorder due to a mutation in fibroblast receptor growth factor genes. Optic
atrophy attributed to optic neuropathy represents the major functional sequella and the major cause of visual loss. Conclusion : Apert syndrome, like all craniosynostotic syndromes, requires a correct management in order to preserve the visual function. Keywords: craniosynostosis, Apert syndrome, decompression surgery, optic atrophy. Journal Tunisien d\'ORL et de chirurgie cervico-faciale Vol. 18 2007: pp. 46-4
Different species of Bradyrhizobium from symbiovars genistearum and retamae nodulate the endemic Retama dasycarpa in the High Atlas Mountains
Retama dasycarpa is an endemic Retama species native to the cold semi-arid bioclimates of the High Atlas Mountains in Morocco. In this work, we analyzed the diversity of the microsymbionts nodulating this plant and their different phenotypic and symbiotic characteristics. Phylogenetic analysis of the 16S rRNA gene revealed that the tested isolates clustered in the Bradyrhizobium genus. Multilocus sequence analyses of four housekeeping genes (recA, gyrB, glnII and atpD) for 12 selected strains grouped them into four clusters close to B. lupini USDA 3051, B. frederickii CNPSo 3446, B. valentinum LmjM3 and B. retamae Ro19. The individual phylogenies of these core genes and the symbiotic genes nodC, nodA and nifH were congruent. These isolates showed a broad host range, being able to nodulate different legume hosts, such as R. sphaerocarpa, R. monosperma, Lupinus luteus, Cytisus grandiflorus and Chamaecytisus albidus, but not Phaseolus vulgaris or Glycine max. They all had a similar metabolic capacity, using the majority of the carbohydrates and amino acids tested as sole sources of carbon and nitrogen. Furthermore, out of the 12 selected strains, some displayed plant growth-promoting features, with six of them solubilizing phosphate and three of them producing siderophores. The present work provides, for the first time, a detailed description about the microsymbionts associated with the endemic legume R. dasycarpa.This work was supported by the Ministry of Higher Education and Innovation. Dr Mouad Lamrabet was granted a fellowship from the PPR2-BIOMIVER project. The authors want to thank all the
people who contributed to this work
Recovery, assessment, and molecular characterization of minor olive genotypes in Tunisia
Olive is one of the oldest cultivated species in the Mediterranean Basin, including Tunisia, where it has a wide diversity, with more than 200 cultivars, of both wild and feral forms. Many minor cultivars are still present in marginal areas of Tunisia, where they are maintained by farmers in small local groves, but they are poorly characterized and evaluated. In order to recover this neglected germplasm, surveys were conducted in different areas, and 31 genotypes were collected, molecularly characterized with 12 nuclear microsatellite (simple sequence repeat (SSR)) markers, and compared with 26 reference cultivars present in the Tunisian National Olive collection. The analysis revealed an overall high genetic diversity of this olive’s germplasm, but also discovered the presence of synonymies and homonymies among the commercialized varieties. The structure analysis showed the presence of different gene pools in the analyzed germplasm. In particular, the marginal germplasm from Ras Jbal and Azmour is characterized by gene pools not present in commercial (Nurseries) varieties, pointing out the very narrow genetic base of the commercialized olive material in Tunisia, and the need to broaden it to avoid the risk of genetic erosion of this species in this country
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Is there sufficient Ensifer and Rhizobium species diversity in UK farmland soils to support red clover (Trifolium pratense), white clover (T. repens), lucerne (Medicago sativa) and black medic (M. lupulina)?
Rhizobia play important roles in agriculture owing to their ability to fix nitrogen through a symbiosis with legumes. The specificity of rhizobia-legume associations means that underused legume species may depend on seed inoculation with their rhizobial partners. For black medic (Medicago lupulina) and lucerne (Medicago sativa) little is known about the natural prevalence of their rhizobial partner Ensifer meliloti in UK soils, so that the need for inoculating them is unclear. We analysed the site-dependence of rhizobial seed inoculation effects on the subsequent ability of rhizobial communities to form symbioses with four legume species (Medicago lupulina, M. sativa, Trifolium repens and T. pratense). At ten organic farms across the UK, a species-diverse legume based mixture (LBM) which included these four species was grown. The LBM seed was inoculated with a mix of commercial inocula specific for clover and lucerne. At each site, soil from the LBM treatment was compared to the soil sampled prior to the sowing of the LBM (the control). From each site and each of the two treatments, a suspension of soils was applied to seedlings of the four legume species and grown in axenic conditions for six weeks. Root nodules were counted and their rhizobia isolated. PCR and sequencing of a fragment of the gyrB gene from rhizobial isolates allowed identification of strains. The number of nodules on each of the four legume species was significantly increased when inoculated with soil from the LBM treatment compared to the control. Both the proportion of plants forming nodules and the number of nodules formed varied significantly by site, with sites significantly affecting the Medicago species but not the Trifolium species. These differences in nodulation were broadly reflected in plant biomass where site and treatment interacted; at some sites there was a significant advantage from inoculation with the commercial inoculum but not at others. In particular, this study has demonstrated the commercial merit of inoculation of lucerne with compatible rhizobia
Catalytic wet peroxide oxidation of phenol over Ce-Zr-modified clays: Effect of the pillaring method
Cd and Ni transport and accumulation in the halophyte Sesuvium portulacastrum: Implication of organic acids in these processes
The implication of organic acids in Cd and Ni translocation was studied in the halophyte species Sesuvium portulacastrum. Citric, fumaric, malic, and ascorbic acids were separated and quantified by HPLC technique in shoots, roots and xylem saps of plants grown on nutrient solutions added with 50 μM Cd, 100 μM Ni and the combination of 50 μM Cd + 100 μM Ni. Results showed that Cd had no significant impact on biomass production while Ni and the combination of both metals drastically affected plant development. Cadmium and Ni concentrations in tissues and xylem sap were higher in plants subjected to individual metal application than those subjected to the combined effect of Cd and Ni suggesting a possible competition between these metals for absorption. Both metals applied separately or in combination induced an increase in citrate concentration in shoots and xylem sap but a decrease of this concentration in the roots. However, a minor relationship was observed between metal application and fumaric, malic, and ascorbic acids. Both observations suggest the implication of citric acid in Cd, Ni translocation and shoot accumulation in S. portulacastrum. The relatively high accumulation of citric acid in xylem sap and shoot of S. portulacastrum could be involved in metal chelation and thus contributes to heavy metal tolerance in this species. © 2015 Mnasri,Ghabriche,Fourati,Zaier,Sabally,Barrington,Lutts, AbdellyandGhnaya
Identification of the symbiovar maamori in Mesorhizobium isolated from nodules of Ononis repens in the Maamora forest (Morocco)
Ononis repens is a legume of the Fabaceae family with great ecological and medicinal importance that grows wild in the Maamora cork oak forest (Rabat, Morocco). The aim of this work was to analyze the diversity, phylogeny, and plant growth-promoting features of the plant root nodules microsymbionts. Out of twenty nodC-containing rhizobial bacteria, four strains, ORM6, ORM8.1, ORM 13.1, and ORM16, were selected for further molecular and phenotypic analyses. All four strains were able to metabolize a wide range of carbohydrates and amino acids as sole carbon and nitrogen sources, respectively, and grew in the presence of different pH, NaCl, and temperature conditions. They also solubilized inorganic phosphate and produced siderophores, and ORM16 was the only strain to produce indol acetic acid. The rrs gene sequence analysis showed that the four strains are members of the Mesorhizobium genus. The individual and concatenated sequences of the recA, glnII, and gyrB housekeeping genes revealed that the strains ORM6 and ORM 13.1 clustered with M. jarvisii ATCC 33,669 and M. intechii BD68 , with which they share 93.3 and 93.7% similarity, respectively; that the strain ORM8.1 affiliated with a group of which the strain M. shonense AC39a was the closest relative species with 84.3% similarity; and that the strain ORM16 clustered with M. australicum LMG24608, with 80.5% similarity. These results suggest that the four O. repens-isolated strains could belong to three putative new genospecies. All four strains nodulated their original host as well as Astragalus gombiformis and A. armatus, but only the strain ORM16 was able to form nodules on Vachellia gummifera. Analysis of the symbiotic nodC gene phylogenies showed that the strains ORM6, ORM8.1, ORM 13.1, and ORM16 formed a group apart from the known symbiovars in the genus Mesorhizobium, for which the name maamori is proposed.This work was supported by Hassan II Academy of Sciences and Technology, in Morocco. Grant P18-RT-1401 from Junta de AndalucĂa is also acknowledged. The authors want to thank all the persons who contributed to this work