Species diversity of phytophagous and predatory mites of wild fruit trees in forest ecosystems of Serbia

Proučavanjem fitofagnih i predatorskih grinja samoniklih vrsta voćaka u šumskim ekosistemima Srbije u periodu 2000-2013 utvrđeno je prisustvo: - 37 vrsta iz natfamilije Eriophyoidea od kojih jedna vrsta pripada familiji Phytoptidae, 31 vrsta familiji Eriophyidae i pet vrsta familiji Diptilomiopidae, svrstanih u 18 rodova. Za faunu Srbije novih je 14 vrsta eriofida. Njihovo prisustvo ustanovljeno je na 41 istraživanom lokalitetu na 21 vrsti biljke hraniteljke. Za tri vrste Leipothrix yipingae, Anthocoptes speciosus i Quadracus ursynus Srbija je zabeležena kao drugi lokalitet posle tipskog lokaliteta. - 20 vrsta iz familije Tetranychidae, od toga šest vrsta pripada potfamiliji Bryobiinae a14 potfamiliji Tetranychinae, grupisanih u osam rodova. Za faunu Srbije zabeleženo je sedam novih vrsta paučinara iz dva roda. Za 13 vrsta paučinara zabeležene su nove biljke hraniteljke. Prikazani su rezultati sa 39 lokaliteta i 19 biljnih vrsta hraniteljki. - 26 vrsta iz familije Phytoseiidae. Potfamilija Amblyseiinae obuhvata osam vrsta, potfamilija Phytoseiinae šest vrsta i potfamilija Typhlodrominae 12 vrsta iz ukupno 11 rodova. Za faunu Srbije novih je 12 vrsta fitozeida. Vrste Phytoseius corniger i Typhloseiulus arzakanicus su prvi put zabeležene u fauni Evrope. Za vrstu Typhloseiulus arzakanicus Srbija je zabeležena kao drugi lokalitet posle tipskog lokaliteta. Prikazani su rezultati sa 44 lokaliteta i 23 biljne vrste. Za sve tri grupe grinja dati su podaci o distribuciji nađenih vrsta u svetu i u Srbiji, odnos prema biljci hraniteljki i moguća štetnost ili korist. Za svaku vrstu dat je taksonomski validni status i tipski podaci, naveden je pun naziv i sinonimi, ukoliko postoje, kao i najznačajnije karakteristike...By studying phytophagous and predatory mites on wild fruit species of forest ecosystems of Serbia in the period 2000-2013 it was determined the presence of: - 37 species from the superfamily Eriophyoidea of which 1 species belongs to the family Phytoptidae, 31 species belong to the family Eriophyidae and 5 species belong to the family Diptilomiopidae grouped into 18 genera. There are 14 new species of eriophyoid mites for fauna of Serbia. Their presence was determined at 41 studying localities on 21 host plants. For 3 species Leipothrix yipingae, Anthocoptes speciosus i Quadracus ursynus Serbia is the second locality after the type locality. - 20 species from the family Tetranychidae of which 6 species belong to the subfamily Bryobiinae and 14 species to the subfamily Tetranychinae that were grouped into 8 genera. 7 new species of spider mites from 2 genera were recorded for the fauna of Serbia. The new host plants were determined for 13 species. The results from 39 localities and 19 host plants are presented. - 26 species from the family Phytoseiidae include 8 species of subfamily Amblyseiinae, 6 species of subfamily Phytoseiinae and 12 species of subfamily Typhlodrominae from a total of 11 genera. For our country 12 species of phytoseiid mites were recorded for the first time. For the fauna of Europe 2 species Phytoseius corniger and Typhloseiulus arzakanicus were recorded for the first time. For one species Typhloseiulus arzakanicus Serbia is the second locality after the type locality. The results from 44 localities and 23 host plants are presented. Data on the distribution of species found in the world and in Serbia, the relationship with the host plant and the possible harm and benefit were given for all three groups of mites..

In vitro procjena antimikrobnog potencijala bakterije Streptococcus uberis izolirane iz autohtonog sira jugoistočne Srbije

Streptococcus uberis is an environmental bacterium responsible for bovine mastitis. It is occasionally described as a human pathogen. In our study, the isolation was undertaken of lactic acid bacteria from a local cheese from Southeastern Serbia, produced in a traditional way. S. uberis (7 isolates) and S. agalactiae (1 isolate) were isolated from the cheese samples taken in the summer. The biochemical and physiological characteristics of the isolates were examined. Using tetracycline, chloramphenicol, novobiocin and rifampicin, the antibiotic susceptibility of the isolates was evaluated. The results demonstrated that all the isolates were susceptible to all the tested antibiotics, with a growth inhibition zone from 36-48 mm. Also, the antagonism was examined of S. uberis KGPMF1-7 and S. agalactiae KFPMF8 isolates on the growth of Escherichia coli ATCC 25922, Proteus mirabilis ATCC 12453, Klebsiella oxytoca KGPMF1, Klebsiella ornithinolytica KGPMF8 and Aeromonas hydrophila, as indicator stains. The results were compared with the activities of chloramphenicol, streptomycin and tetracycline on the tested indicator stains. The strongest antagonism was demonstrated by all Streptococcus isolates on the growth of K. oxytoca KGPMF1 (growth inhibition zone from 12-20 mm) and the A. hydrophila (growth inhibition zone from 13-20 mm). When these results were compared with the results of the sensitivity of tested indicator stains to antibiotics, S. uberis KGPMF1-7 and S. agalactiae KGPMF8 isolates showed a moderate antagonistic effect. Due to the specific way cheese is made in from Sokobanja, these isolates probably originate from cows’ udders.Streptococcus uberis jest okolišna bakterija odgovorna za mastitis kod goveda, a povremeno se opisuje kao patogen u ljudi. U ovom su istraživanju izolirane bakterije mliječne kiseline iz autohtonog sira jugoistočne Srbije, proizvedenog na tradicionalni način. S. uberis (7 izolata) i S. agalactiae (1 izolat) izolirani su iz uzoraka sira prikupljenih tijekom ljeta. Analizirane su biokemijske i fiziološke značajke izolata. Koristeći se tetraciklinom, kloramfenikolom, novobiocinom i rifampicinom, procijenjena je osjetljivost izolata na antibiotike. Rezultati su pokazali da su svi izolati osjetljivi na sve istražene antibiotike, sa zonom inhibicije rasta od 36 do 48 mm. Također, istražen je antagonizam S. uberis KGPMF1-7 i S. agalactiae KFPMF8 izolata na rast Escherichia coli ATCC 25922, Proteus mirabilis ATCC 12453, Klebsiella oxytoca KGPMF1, Klebsiella ornithinolytica KGPMF8 i Aeromonas hydrophila kao indikatorskih sojeva. Rezultati su uspoređeni s aktivnostima kloramfenikola, streptomicina i tetraciklina na testiranim indikatorskim sojevima. Najjači antagonizam pokazali su svi Streptococcus izolati na rast K. oxytoca KGPMF1 (zona inhibicije rasta od 12 do 20 mm) i A. hydrophila (zona inhibicije rasta od 13 do 20 mm). Usporedbom tih rezultata s rezultatima osjetljivosti istraženih indikatorskih sojeva na antibiotike, za S. uberis KGPMF1-7 i S. agalactiae KGPMF8 izolate utvrđen je umjeren antagonistički učinak. Ti izolati vjerojatno potječu iz vimena krava i u siru iz Sokobanje prisutni su zbog specifičnosti njegove pripreme

Antagonistički potencijal i sposobnost stvaranja biofilma bakterija Enterococcus spp. izoliranih iz sira u Srbiji

In this paper, the presence, biochemical and physiological characteristics of the members of genus Enterococcus, isolated from traditionally made Serbian cheese, were investigated. The members were identified as: Enterococcus faecium (8 isolates), E. faecalis (14 isolates), E. hirae (4 isolates) and E. durans (4 isolates), using the biochemical tests and MALDI-TOF mass spectrometry. All the tested isolates showed good acidification ability in pure and enriched milk. The antagonism of enterococci on the growth of Escherichia coli ATCC 25922, Proteus mirabilis ATCC 12453, Klebsiella oxytoca KGPMF1, Klebsiella ornithinolytica KGPMF8 and Aeromonas hydrophila, as well as their ability to form biofilms, were examined. The tested isolates showed moderate inhibitory activity (10-22 mm) on the growth of the indicator strains. Among all the isolates, only E. hirae KGPMF9 and E. faecium KGPMF14 showed the ability of biofilm formation. The results provide a basis for further research into the possible practical application of the isolated enterococci.U ovom je radu istražena prisutnost te biokemijske i fiziološke značajke bakterija iz roda Enterococcus, izoliranih iz domaćeg sira u Srbiji, proizvedenoga na tradicionalan način. Biokemijskim testom i masenom spektrometrijom MALDI-TOF dokazani su Enterococcus faecium (8 izolata), E. faecalis (14 izolata), E. hirae (4 izolata) i E. durans (4 izolata). Svi su analizirani izolati pokazali dobru sposobnost acidifikacije u sirovu i obogaćenom mlijeku. Istraženi su antagonizam enterokoka na rast bakterija Escherichia coli ATCC 25922, Proteus mirabilis ATCC 12453, Klebsiella oxytoca KGPMF1, Klebsiella ornithinolytica KGPMF8 i Aeromonas hydrophila te njihova sposobnost stvaranja biofilma. Analizirani izolati pokazali su umjerenu inhibicijsku aktivnost (10 - 22 mm) u rastu navedenih sojeva. Među izolatima samo su E. hirae KGPMF9 i E. faecium KGPMF14 pokazali sposobnost stvaranja biofilma. Rezultati ove studije daju temelj za daljnja istraživanja mogućnosti praktične primjene izoliranih enterokoka

The influence of environmental factors on the planktonic growth and biofilm formation of Escherichia coli

In this study, the effects of environmental factors (different media, temperature, pH, salt and sugar concentrations) on the planktonic growth, biofilm formation and formed biofilm of Escherichia coli KGPMF 16 and Escherichia coli KGPMF 17 were investigated. Tested bacteria were isolated from traditionally made cheese produced in Southeastern Serbia (Sokobanja region). The influence on planktonic growth, biofilm formation and formed biofilm was determined using spectrophotometric method. The limiting factors for the planktonic growth and biofilm formation were temperature of 4 °C and all tested concentrations of salt. The growth of tested bacteria was higher in media enriched with lactose than in media containing glucose. TSB was more congenial media to the planktonic growth of bacteria than MHB broth. None of the tested bacteria demonstrated the ability to form biofilm at 4 °C and 44 °C. Only E. coli KGPMF 17 showed ability to form biofilm in TSB at 37 °C. Different concentrations of salt, glucose and lactose exhibited inhibitory effect on biofilm formation, but all tested concentrations of lactose showed stimulating effect on formed biofilm of E. coli KGPMF 17. These results contribute to better understanding of the effects of environmental factors on the development of E. coli in cheese

Volatiles of Thymus serpyllum Obtained by Three Different Methods

Volatiles of Thymus serpyllum L. were obtained by hydrodistillation (HD), simultaneous hydrodistillation, and extraction (SDE) and static headspace gas chromatography-mass spectrometry analysis (head space [HS]), respectively. Monoterpenes were the most dominant in all 3 techniques (84.8%-94.2%). Essential oil profiles obtained by HD and SDE were similar, with oxygenated monoterpenes being the most abundant (up to 75.4%). In HS volatiles of T. serpyllum monoterpene hydrocarbons strongly dominated (94.2%). The main compounds were α-terpinyl acetate (HD and SDE) and myrcene (HS)

Supplementary material for the article: Milošev, M. Z.; Jakovljević, K.; Joksović, M. D.; Stanojković, T.; Matić, I. Z.; Perović, M.; Tešić, V.; Kanazir, S.; Mladenović, M.; Rodić, M. V.; et al. Mannich Bases of 1,2,4-Triazole3-Thione Containing Adamantane Moiety: Synthesis, Preliminary Anticancer Evaluation, and Molecular Modeling Studies. Chemical Biology and Drug Design 2017, 89 (6), 943–952. https://doi.org/10.1111/cbdd.12920

Supporting Information: [https://doi.org/10.1111/cbdd.12920]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2482]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3221

Supplementary material for the article: Milošev, M. Z.; Jakovljević, K.; Joksović, M. D.; Stanojković, T.; Matić, I. Z.; Perović, M.; Tešić, V.; Kanazir, S.; Mladenović, M.; Rodić, M. V.; et al. Mannich Bases of 1,2,4-Triazole3-Thione Containing Adamantane Moiety: Synthesis, Preliminary Anticancer Evaluation, and Molecular Modeling Studies. Chemical Biology and Drug Design 2017, 89 (6), 943–952. https://doi.org/10.1111/cbdd.12920

Supporting Information: [https://doi.org/10.1111/cbdd.12920]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2482]Related to accepted version: [http://cherry.chem.bg.ac.rs/handle/123456789/3221

Species diversity of phytophagous and predatory mites of wild fruit trees in forest ecosystems of Serbia

Proučavanjem fitofagnih i predatorskih grinja samoniklih vrsta voćaka u šumskim ekosistemima Srbije u periodu 2000-2013 utvrđeno je prisustvo: - 37 vrsta iz natfamilije Eriophyoidea od kojih jedna vrsta pripada familiji Phytoptidae, 31 vrsta familiji Eriophyidae i pet vrsta familiji Diptilomiopidae, svrstanih u 18 rodova. Za faunu Srbije novih je 14 vrsta eriofida. Njihovo prisustvo ustanovljeno je na 41 istraživanom lokalitetu na 21 vrsti biljke hraniteljke. Za tri vrste Leipothrix yipingae, Anthocoptes speciosus i Quadracus ursynus Srbija je zabeležena kao drugi lokalitet posle tipskog lokaliteta. - 20 vrsta iz familije Tetranychidae, od toga šest vrsta pripada potfamiliji Bryobiinae a14 potfamiliji Tetranychinae, grupisanih u osam rodova. Za faunu Srbije zabeleženo je sedam novih vrsta paučinara iz dva roda. Za 13 vrsta paučinara zabeležene su nove biljke hraniteljke. Prikazani su rezultati sa 39 lokaliteta i 19 biljnih vrsta hraniteljki. - 26 vrsta iz familije Phytoseiidae. Potfamilija Amblyseiinae obuhvata osam vrsta, potfamilija Phytoseiinae šest vrsta i potfamilija Typhlodrominae 12 vrsta iz ukupno 11 rodova. Za faunu Srbije novih je 12 vrsta fitozeida. Vrste Phytoseius corniger i Typhloseiulus arzakanicus su prvi put zabeležene u fauni Evrope. Za vrstu Typhloseiulus arzakanicus Srbija je zabeležena kao drugi lokalitet posle tipskog lokaliteta. Prikazani su rezultati sa 44 lokaliteta i 23 biljne vrste. Za sve tri grupe grinja dati su podaci o distribuciji nađenih vrsta u svetu i u Srbiji, odnos prema biljci hraniteljki i moguća štetnost ili korist. Za svaku vrstu dat je taksonomski validni status i tipski podaci, naveden je pun naziv i sinonimi, ukoliko postoje, kao i najznačajnije karakteristike...By studying phytophagous and predatory mites on wild fruit species of forest ecosystems of Serbia in the period 2000-2013 it was determined the presence of: - 37 species from the superfamily Eriophyoidea of which 1 species belongs to the family Phytoptidae, 31 species belong to the family Eriophyidae and 5 species belong to the family Diptilomiopidae grouped into 18 genera. There are 14 new species of eriophyoid mites for fauna of Serbia. Their presence was determined at 41 studying localities on 21 host plants. For 3 species Leipothrix yipingae, Anthocoptes speciosus i Quadracus ursynus Serbia is the second locality after the type locality. - 20 species from the family Tetranychidae of which 6 species belong to the subfamily Bryobiinae and 14 species to the subfamily Tetranychinae that were grouped into 8 genera. 7 new species of spider mites from 2 genera were recorded for the fauna of Serbia. The new host plants were determined for 13 species. The results from 39 localities and 19 host plants are presented. - 26 species from the family Phytoseiidae include 8 species of subfamily Amblyseiinae, 6 species of subfamily Phytoseiinae and 12 species of subfamily Typhlodrominae from a total of 11 genera. For our country 12 species of phytoseiid mites were recorded for the first time. For the fauna of Europe 2 species Phytoseius corniger and Typhloseiulus arzakanicus were recorded for the first time. For one species Typhloseiulus arzakanicus Serbia is the second locality after the type locality. The results from 44 localities and 23 host plants are presented. Data on the distribution of species found in the world and in Serbia, the relationship with the host plant and the possible harm and benefit were given for all three groups of mites..

Species diversity of phytophagous and predatory mites of wild fruit trees in forest ecosystems of Serbia

Proučavanjem fitofagnih i predatorskih grinja samoniklih vrsta voćaka u šumskim ekosistemima Srbije u periodu 2000-2013 utvrđeno je prisustvo: - 37 vrsta iz natfamilije Eriophyoidea od kojih jedna vrsta pripada familiji Phytoptidae, 31 vrsta familiji Eriophyidae i pet vrsta familiji Diptilomiopidae, svrstanih u 18 rodova. Za faunu Srbije novih je 14 vrsta eriofida. Njihovo prisustvo ustanovljeno je na 41 istraživanom lokalitetu na 21 vrsti biljke hraniteljke. Za tri vrste Leipothrix yipingae, Anthocoptes speciosus i Quadracus ursynus Srbija je zabeležena kao drugi lokalitet posle tipskog lokaliteta. - 20 vrsta iz familije Tetranychidae, od toga šest vrsta pripada potfamiliji Bryobiinae a14 potfamiliji Tetranychinae, grupisanih u osam rodova. Za faunu Srbije zabeleženo je sedam novih vrsta paučinara iz dva roda. Za 13 vrsta paučinara zabeležene su nove biljke hraniteljke. Prikazani su rezultati sa 39 lokaliteta i 19 biljnih vrsta hraniteljki. - 26 vrsta iz familije Phytoseiidae. Potfamilija Amblyseiinae obuhvata osam vrsta, potfamilija Phytoseiinae šest vrsta i potfamilija Typhlodrominae 12 vrsta iz ukupno 11 rodova. Za faunu Srbije novih je 12 vrsta fitozeida. Vrste Phytoseius corniger i Typhloseiulus arzakanicus su prvi put zabeležene u fauni Evrope. Za vrstu Typhloseiulus arzakanicus Srbija je zabeležena kao drugi lokalitet posle tipskog lokaliteta. Prikazani su rezultati sa 44 lokaliteta i 23 biljne vrste. Za sve tri grupe grinja dati su podaci o distribuciji nađenih vrsta u svetu i u Srbiji, odnos prema biljci hraniteljki i moguća štetnost ili korist. Za svaku vrstu dat je taksonomski validni status i tipski podaci, naveden je pun naziv i sinonimi, ukoliko postoje, kao i najznačajnije karakteristike...By studying phytophagous and predatory mites on wild fruit species of forest ecosystems of Serbia in the period 2000-2013 it was determined the presence of: - 37 species from the superfamily Eriophyoidea of which 1 species belongs to the family Phytoptidae, 31 species belong to the family Eriophyidae and 5 species belong to the family Diptilomiopidae grouped into 18 genera. There are 14 new species of eriophyoid mites for fauna of Serbia. Their presence was determined at 41 studying localities on 21 host plants. For 3 species Leipothrix yipingae, Anthocoptes speciosus i Quadracus ursynus Serbia is the second locality after the type locality. - 20 species from the family Tetranychidae of which 6 species belong to the subfamily Bryobiinae and 14 species to the subfamily Tetranychinae that were grouped into 8 genera. 7 new species of spider mites from 2 genera were recorded for the fauna of Serbia. The new host plants were determined for 13 species. The results from 39 localities and 19 host plants are presented. - 26 species from the family Phytoseiidae include 8 species of subfamily Amblyseiinae, 6 species of subfamily Phytoseiinae and 12 species of subfamily Typhlodrominae from a total of 11 genera. For our country 12 species of phytoseiid mites were recorded for the first time. For the fauna of Europe 2 species Phytoseius corniger and Typhloseiulus arzakanicus were recorded for the first time. For one species Typhloseiulus arzakanicus Serbia is the second locality after the type locality. The results from 44 localities and 23 host plants are presented. Data on the distribution of species found in the world and in Serbia, the relationship with the host plant and the possible harm and benefit were given for all three groups of mites..

Extracts of Agrimonia eupatoria L. as sources of biologically active compounds and evaluation of their antioxidant, antimicrobial, and antibiofilm activities

In this study, we determined the concentration of total phenols, flavonoids, tannins, and proanthocyanidins in the water, diethyl ether, acetone, and ethanol extracts of Agrimonia eupatoria L. We also investigated the antioxidant activity of these extracts using two methods [2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power] and their in vitro antimicrobial (antibacterial and antifungal) activity on some selected species of bacteria and fungi. In addition, the effects of the acetone and water extracts on the inhibition of biofilm formation of Proteus mirabilis and Pseudomonas aeruginosa were investigated using the crystal violet method. The concentration of total phenols was measured according to the Folin–Ciocalteu method and the values obtained ranged from 19.61 mgGA/g to 220.31 mgGA/g. The concentration of flavonoids was examined by the aluminum chloride method and the values obtained ranged from 20.58 mgRU/g to 97.06 mgRU/g. The total tannins concentration was measured by the polyvinylpolypyrrolidone method and the values obtained ranged from 3.06 mgGA/g to 207.27 mgGA/g. The concentration of proanthocyanidins was determined by the butanol–HCl method and the values obtained ranged from 4.15 CChE/g to 103.72 CChE/g. Among the various extracts studied, the acetone extract exhibited good antioxidant activity (97.13%, as determined by the DPPH method). The acetone extract was active in the absorbance value range from 2.2665 to 0.2495 (as determined by the reducing power method). The strongest antimicrobial activity was detected on G+ bacteria, especially on probiotic species, and the acetone extract demonstrated the highest activity. Biofilm inhibitory concentration required to reduce biofilm coverage by 50% values for acetone extract was 4315 μg/mL for P. mirabilis and 4469.5 μg/mL for P. aeruginosa. The results provide a basis for further research of this plant species