Amino Acids Involved in Polyphosphate Synthesis and Its Mobilization Are Distinct in Polyphosphate Kinase-1 from Mycobacterium tuberculosis

In bacteria polyphosphates (poly-P) are involved in cellular metabolism and development especially during stress. The enzyme, principally involved in polyphosphate biosynthesis and its mobilization leading to generation of NTPs, is known as polyphosphate kinase (PPK). PPK1 crystal structure since it owes 68% amino acid sequence similarity with mPPK1. Interestingly, our results revealed that mutations in mPPK1 affecting poly-P synthesis always affected its ATP synthesizing ability; however, the reverse may not be true. only

Magnetic structure of EuFe2As2 determined by single crystal neutron diffraction

Among various parent compounds of iron pnictide superconductors, EuFe2As2 stands out due to the presence of both spin density wave of Fe and antiferromagnetic ordering (AFM) of the localized Eu2+ moment. Single crystal neutron diffraction studies have been carried out to determine the magnetic structure of this compound and to investigate the coupling of two magnetic sublattices. Long range AFM ordering of Fe and Eu spins was observed below 190 K and 19 K, respectively. The ordering of Fe2+ moments is associated with the wave vector k = (1,0,1) and it takes place at the same temperature as the tetragonal to orthorhombic structural phase transition, which indicates the strong coupling between structural and magnetic components. The ordering of Eu moment is associated with the wave vector k = (0,0,1). While both Fe and Eu spins are aligned along the long a axis as experimentally determined, our studies suggest a weak coupling between the Fe and Eu magnetism.Comment: 7 pages, 7 figure

Field induced spin reorientation and giant spin-lattice coupling in EuFe2As2

We have studied a EuFe2As2 single crystal by neutron diffraction under magnetic fields up to 3.5 T and temperatures down to 2 K. A field induced spin reorientation is observed in the presence of a magnetic field along both the a and c axes, respectively. Above critical field, the ground state antiferromagnetic configuration of Eu$^{2+}$ moments transforms into a ferromagnetic structure with moments along the applied field direction. The magnetic phase diagram for Eu magnetic sublattice in EuFe2As2 is presented. A considerable strain ($\sim$0.9%) is induced by the magnetic field, caused by the realignment of the twinning structure. Furthermore, the realignment of the twinning structure is found to be reversible with the rebound of magnetic field, which suggested the existence of magnetic shape-memory effect. The Eu moment ordering exhibits close relationship with the twinning structure. We argue that the Zeeman energy in combined with magnetic anisotropy energy is responsible for the observed spin-lattice coupling.Comment: 5 pages, 4 figure

Narcotic Drug Detection and Identification through Synchronous Fluorescence Technique

Illegal production of potentially hazardous narcotics as well as various psychotropic drugs is being carried out in the neighbouring countries and international market which is causing narco-terrorism a global havoc. Compared with traditional/natural drugs the synthetic drugs are more smuggled nowadays. Because of the vulnerability of the borders to drug trafficking, India has increased surveillance at borders and coasts and thus has tackled the problem to some extent. Authors developing a Narcotic drug sensor (point sensor) for detection and identification of Narcotic drugs based on Laser Induced Fluorescence more specifically Synchronous Fluorescence Spectroscopy in an effort. Some narcotic samples viz. Caffeine, Quinine and Tramadol were considered for the study. The fluorescence spectrum was studied using our own laboratory-based sensor incorporating charge coupled device-based Spectrometer and Laser source (266 nm wavelength) and commercial system from ‘Horiba Scientific’ incorporating Photomultiplier tube and Xe lamp source

Laser isotope separation of <SUP>13</SUP>C: a comparative study

IR laser chemistry of (CF3Br/Cl2) mixture and neat CF2HCl are examined in the context of 13C enrichment. Decomposition extent, enrichment factor and energy absorbed are measured for both systems at their respective optimum conditions. A direct comparison is obtained by keeping extraneous factors such as laser, its pulse duration, cell, irradiation geometry etc. the same. The halogen scavenged CF3Br MPD requires lower fluence compared to neat CF2HCl irradiation. Overall throughput for a product with 60-65% 13C content in a single stage is the same for both systems requiring a similar amount of energy. However, at lower enrichment levels, CF2HCl MPD is better than (CF3Br/Cl2) photolysis in terms of both product yield and energy absorption

Redshifted formaldehyde from the gravitational lens B0218+357

The gravitational lens toward B0218+357 offers the unique possibility to study cool moderately dense gas with high sensitivity and angular resolution in a cloud that existed half a Hubble time ago. Observations of the radio continuum and six formaldehyde (H2CO) lines were carried out with the VLA, the Plateau de Bure interferometer, and the Effelsberg 100-m telescope. Three radio continuum maps indicate a flux density ratio between the two main images, A and B, of ~ 3.4 +/- 0.2. Within the errors the ratio is the same at 8.6, 14.1, and 43 GHz. The 1_{01}-0_{00} line of para-H2CO is shown to absorb the continuum of image A. Large Velocity Gradient radiative transfer calculations are performed to reproduce the optical depths of the observed two cm-wave "K-doublet" and four mm-wave rotational lines. These calculations also account for a likely frequency-dependent continuum cloud coverage. Confirming the diffuse nature of the cloud, an n(H2) density of < 1000 cm^{-3} is derived, with the best fit suggesting n(H2) ~ 200 cm^{-3}. The H2CO column density of the main velocity component is ~5 * 10^{13} cm^{-2}, to which about 7.5 * 10^{12} cm^{-2} has to be added to also account for a weaker feature on the blue side, 13 km/s apart. N(H2CO)/N(NH3) ~ 0.6, which is four times less than the average ratio obtained from a small number of local diffuse (galactic) clouds seen in absorption. The ortho-to-para H2CO abundance ratio is 2.0 - 3.0, which is consistent with the kinetic temperature of the molecular gas associated with the lens of B0218+357. With the gas kinetic temperature and density known, it is found that optically thin transitions of CS, HCN, HNC, HCO+, and N2H+ (but not CO) will provide excellent probes of the cosmic microwave background at redshift z=0.68.Comment: Accepted for A&A, 8 Pages, 3 Figures, 5 Table