The BNL AGS accelerator complex status and future plans

This paper describes the present performance and capability of the BNL AGS accelerator complex and possible future intensity upgrade plans. In 1995, the AGS reached its design upgrade goal of 6.0 x 10(exp 13) ppp with the Booster. The AGS with a new fast extracted beam (FEB) system is able to perform single bunch multiple extraction at 30 Hz per AGS cycle for the g-2 experiment and for RHIC injection

Purification and Comparison of Peroxisomal and Cytosolic Catalases from a Methanol-Grown Yeast, Kloeckera sp. 2201

Catalases were purified from a peroxisome-containing particulate fraction and a cytosolic fraction of methanol-grown Kloeckera sp. 2201 cells after subcellular fractionation. No difference was observed between the enzymes in the behaviours on column chromatographies, molecular mass of the subunits (M, 62, 000 daltons), and terminal amino acids, alanine. In addition, similar patterns were obtained with the peroxisomal and cytosolic enzymes on sodium dodecylsulfate/polyacrylamide slab-gel electrophoresis of the peptide fragments prepared by partial digestion with Staphylococcus aureus V 8 protease and papain. These results indicate that cytosolic catalase, even if functional, essentially has identical properties with the peroxisomal one in spite of the different subcellular distribution

Isolation of the Gene Encoding Yeast Peroxisomal Isocitrate Lyase by a Combination of the Plaque Hybridization with Non-Radioactive Probes and the Amplification of Phages in a Small Scale

A genomic DNA encoding isocitrate lyase, one of peroxisomal enzymes, was successfully isolated from an n-alkane-utilizable yeast genomic library prepared in a λEMBL phage by a combination of the plaque hybridization with a non-radioactive, biotin labeled, cDNA and the amplification of the phages in a small scale. Three clones, partially overlapping, with sizes of about 11, 13 and 16 kbp respectively were finally obtained. The genomic Southern blot analysis using the biotin-labeled probe suggested the presence of one isocitrate lyase gene in the genomic DNA

Expression Cloning of Catalase Genomic Gene : Genomic DNA Expression Library of Candida boidinii in Saccharomyces cerevisiae

The genomic DNA expression library of a methylotrophic yeast, Candida boidinii (Kloeckera sp.) 2201, was prepared in Saccharomyces cerevisiae by the electroporation method. Five transformants harbouring a catalase gene were independently isolated with an anti-C. boidinii catalase antibody from this library. Furthermore, exhibition of catalase activity in these transformants demonstrated that C. boidinii genes could sufficiently function even in S. cerevisiae

Single Photon Emission Computed Tomography of Neonatal Periventricular Leukomalacia

We describe herein single photon emission computed tomography (SPECT) with 99mTc HM-PAO of a patient with neonatal cystic periventricular leukomalacia (PVL). The patient, who was a low birth weight infant of 27 weeks gestation weighing 1,290 g, had suffered from respiratory distress syndrome. Following surfactant replacement therapy, mechanical ventilation were carried out six days after birth. Serial cranial ultrasonography (US) showed periventricular high-echoic lesions followed by cyst formation at three weeks of age. Although cystic PVL was markedly observed on both cranial US and MRI, a SPECT study initially revealed no abnormal distribution of rCBF. A chronological SPECT study at nine months of age revealed decreased rCBF of 10% in the left hemisphere as compared to the contralateral side. At the time decreased rCBF was observed on SPECT, periventricular cyst formation tended to disappear on simultaneous MRI. It is known that PVL is an infarction which affects all the cellular elements of the white matter and may lead to abnormal myelination. SPECT studies may also useful to clarify the cerebral function in neonatal PVL

Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: a role of rab3B in secretion of GH through porosome.

Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome

Evaluation of Source Generators in Partial Seizures : Availability of Neuroradiological Images Superimposed on the Dipole Tracing

To clarify the anatomical localization of epileptic foci in childhood partial seizures, the source generators of interictal focal spikes were analyzed using a dipole tracing method and the effectiveness of a composite image diagnostic technique using neuroradiological images obtained by magnetic resonance imaging and single photon emission computed tomography was evaluated. The localization of electric source generators approximately reflects the abnormal lesions on neuroradiological images. However, the source generators tended to be located in the deep and/or peripheral parts of the abnormal regions. Even though systemic shifts in the position of optimal dipoles from their true positions is a limitation that must be taken into consideration, composite image diagnostic technique may be useful for evaluating the epileptogenic regions in partial seizures, and particularly, in children where noninvasive methods are preferable

Development of Management File Sharing System not requiring File Server Specialized for Server Management System

In the file servers that are normally used to share management files in server management systems, complicated controls, such as management file sharing methods, are required both as preparation against failure and to ensure file server redundancy. To facilitate such efforts, we propose a management file sharing system (MFSS) for server management systems that does not require a specialized file server. Herein, the detailed configuration of our proposed MFSS, its operation at the times of server failure and recovery, and its overall performance are discussed and evaluated. Then, using various examples, including a high-availability server system and a power-saving server system that utilize different management timings, we demonstrate the utility of our proposed MFSS.departmental bulletin pape

ALCHEMI Finds a “Shocking” Carbon Footprint in the Starburst Galaxy NGC 253

The centers of starburst galaxies may be characterized by a specific gas and ice chemistry due to their gas dynamics and the presence of various ice desorption mechanisms. This may result in a peculiar observable composition. We analyse the abundances of CO2, a reliable tracer of ice chemistry, from data collected as part of the Atacama Large Millimeter/submillimeter Array large program ALCHEMI, a wide-frequency spectral scan toward the starburst galaxy NGC 253 with an angular resolution of 1.″6. We constrain the CO2 abundances in the gas phase using its protonated form HOCO+. The distribution of HOCO+ is similar to that of methanol, which suggests that HOCO+ is indeed produced from the protonation of CO2 sublimated from ice. The HOCO+ fractional abundances are found to be (1-2) 7 10−9 at the outer part of the central molecular zone (CMZ), while they are lower (∼10−10) near the kinematic center. This peak fractional abundance at the outer CMZ is comparable to that in the Milky Way CMZ, and orders of magnitude higher than that in Galactic disk, star-forming regions. From the range of HOCO+/CO2 ratios suggested from chemical models, the gas-phase CO2 fractional abundance is estimated to be (1-20) 7 10−7 at the outer CMZ, and orders of magnitude lower near the center. We estimate the CO2 ice fractional abundances at the outer CMZ to be (2-5) 7 10−6 from the literature. A comparison between the ice and gas CO2 abundances suggests an efficient sublimation mechanism. This sublimation is attributed to large-scale shocks at the orbital intersections of the bar and CMZ