Complete Genetic Correction of iPS Cells From Duchenne Muscular Dystrophy

Human artificial chromosome (HAC) has several advantages as a gene therapy vector, including stable episomal maintenance that avoids insertional mutations and the ability to carry large gene inserts including the regulatory elements. Induced pluripotent stem (iPS) cells have great potential for gene therapy, as such cells can be generated from the individual's own tissues, and when reintroduced can contribute to the specialized function of any tissue. As a proof of concept, we show herein the complete correction of a genetic deficiency in iPS cells derived from Duchenne muscular dystrophy (DMD) model (mdx) mice and a human DMD patient using a HAC with a complete genomic dystrophin sequence (DYS-HAC). Deletion or mutation of dystrophin in iPS cells was corrected by transferring the DYS-HAC via microcell-mediated chromosome transfer (MMCT). DMD patient- and mdx-specific iPS cells with the DYS-HAC gave rise to differentiation of three germ layers in the teratoma, and human dystrophin expression was detected in muscle-like tissues. Furthermore, chimeric mice from mdx-iPS (DYS-HAC) cells were produced and DYS-HAC was detected in all tissues examined, with tissue-specific expression of dystrophin. Therefore, the combination of patient-specific iPS cells and HAC-containing defective genes represents a powerful tool for gene and cell therapies

ヘイメン ノ セイソクブンカツ ニ モトズク ハーフトーンセル ノ セッケイ ： ドウブツ ノ ケイジョウ オ モツ モウテン ノ サクセイ

Halftone dots used in printing are usually square or circular. This paper describes a method to create halftone dots in the shape of a motif such as an animal. The method uses a tiling technique, which is based on four kinds of congruent transformation : translation, rotation, reflection and glide reflection. Tiling with quadrangle was examind and found to be divided into twelve groups. 0ne of the groups was selected for a design of a motif. In order to generate the motif a threshold matrix was used, which was designed so that a halftone dot appeared to be a different animal according to its tone value

Isolation of deuterated histones from yeast grown on media dissolved in 2H2O

AbstractWe have succeeded in growing Saccharomyces cerevisiae (baker's yeast) on media containing 2H2O and isolating the core histones highly deuterated in the non-exchangeable positions. The deuterated histones obtained here are of great value for their possible widespread use for structural studies of chromatin