Eksperimentalna infektivna pneumokonioza

Quartz and coal-mine dusts were introduced into the lungs of rats by inhalation, in a high concentration, for four months, and then groups of these dusted animals were infected with a virulent strain of human tubercle bacillus (37 Rv). Dusting was continued for a further period of ten months in the case of coal-mine dust. No variation in lung pathology was seen in the quartz groups, although both produced classical silicotic nodules. In the coal-mine dust the infective group showed slightly more fibrosis, diffuse in nature, than either the noninfected or the tuberculous control group. These are preliminary experiments, and the results must be considered as both tentative and not very conclusive. Work is being continued.Prašina kvarca i ugljenokopna prašina bile su unesene inhalacijom u pluća štakora. Koncentracija je bila visoka, a ekspozicija je trajala 4 mjeseca Nakon toga su grupe zaprašenih životinja bile inficirane virulentnim sojom humanog tuberkuloznog bacila (H 37 Rv). Kod grupe životinja, koje su bile izvrgnute djelovanju ugljenokopne prašine, nastavljeno je zaprašivanje daljih 10 mjeseci. U kvarcnim grupama nije bilo razlike s obzirom na patologiju pluća, iako je u obje grupe došlo do stvaranja klasičnih silikotičnih čvorova. U grupi životinja, koje su bile izvrgnute ugljenokopnoj prašini, nađeno je nešto više fibroze, koja je bila difuzna, nego bilo u neinficiranoj ili tuberkuloznoj kontrolnoj grupi. Ti eksperimenti su preliminarni i ne treba ih smatrati previše konkluzivnima. Rad se nastavlja

Persister populations of Mycobacterium tuberculosis in sputum that grow in liquid but not on solid culture media

OBJECTIVES : Can the characteristics of persisters in cultures of Mycobacterium tuberculosis also be found in bacilli from the sputum of pulmonary tuberculosis patients? The objective of this study was to explore whether the ability of persisters to grow in liquid but not on solid culture media, as in 100 day static cultures, can also be found in bacilli in sputum. METHODS : Serial dilutions of homogenized sputum obtained from patients before or during the first week of treatment were inoculated into broths to estimate the probable number of organisms and onto plates to give colony counts. RESULTS : Cultures in broths grew slowly to reach a maximal count at 12 weeks of probable numbers about 10-fold higher than the colony counts on plates, which did not grow beyond the initial count at 3-4 weeks. No such excess growth in liquid medium was found with control log-phase cultures. CONCLUSIONS : About 90% of the bacilli in sputum are persisters that can grow in liquid media but not on solid plates.Sputum specimens were examined as part of the quality assurance programme of the Oflotub clinical trial,14 financially supported by a grant (ICA4-CT2002-10057) from the European Commission and another (OD/TS-04-00291) from the WHO special programme for research and training in tropical diseases (TDR).http://jac.oxfordjournals.orghb201

Active Brownian Particles. From Individual to Collective Stochastic Dynamics

We review theoretical models of individual motility as well as collective dynamics and pattern formation of active particles. We focus on simple models of active dynamics with a particular emphasis on nonlinear and stochastic dynamics of such self-propelled entities in the framework of statistical mechanics. Examples of such active units in complex physico-chemical and biological systems are chemically powered nano-rods, localized patterns in reaction-diffusion system, motile cells or macroscopic animals. Based on the description of individual motion of point-like active particles by stochastic differential equations, we discuss different velocity-dependent friction functions, the impact of various types of fluctuations and calculate characteristic observables such as stationary velocity distributions or diffusion coefficients. Finally, we consider not only the free and confined individual active dynamics but also different types of interaction between active particles. The resulting collective dynamical behavior of large assemblies and aggregates of active units is discussed and an overview over some recent results on spatiotemporal pattern formation in such systems is given.Comment: 161 pages, Review, Eur Phys J Special-Topics, accepte

Eksperimentalna infektivna pneumokonioza

Quartz and coal-mine dusts were introduced into the lungs of rats by inhalation, in a high concentration, for four months, and then groups of these dusted animals were infected with a virulent strain of human tubercle bacillus (37 Rv). Dusting was continued for a further period of ten months in the case of coal-mine dust. No variation in lung pathology was seen in the quartz groups, although both produced classical silicotic nodules. In the coal-mine dust the infective group showed slightly more fibrosis, diffuse in nature, than either the noninfected or the tuberculous control group. These are preliminary experiments, and the results must be considered as both tentative and not very conclusive. Work is being continued.Prašina kvarca i ugljenokopna prašina bile su unesene inhalacijom u pluća štakora. Koncentracija je bila visoka, a ekspozicija je trajala 4 mjeseca Nakon toga su grupe zaprašenih životinja bile inficirane virulentnim sojom humanog tuberkuloznog bacila (H 37 Rv). Kod grupe životinja, koje su bile izvrgnute djelovanju ugljenokopne prašine, nastavljeno je zaprašivanje daljih 10 mjeseci. U kvarcnim grupama nije bilo razlike s obzirom na patologiju pluća, iako je u obje grupe došlo do stvaranja klasičnih silikotičnih čvorova. U grupi životinja, koje su bile izvrgnute ugljenokopnoj prašini, nađeno je nešto više fibroze, koja je bila difuzna, nego bilo u neinficiranoj ili tuberkuloznoj kontrolnoj grupi. Ti eksperimenti su preliminarni i ne treba ih smatrati previše konkluzivnima. Rad se nastavlja

Use of the Phoenix system for bacteriology

A dedicated microbiology data processing system with remote batched job entry to an obsolete computer, has been superseded by the inclusion of bacteriology in an on-line interactive clinical pathology system which had previously incorporated chemical pathology and haematology. The original Phoenix system has been adapted to allow for the entry of bacteriology data using mnemonic codes and to deal with the problems caused by the longer processing time of bacteriology specimens. Particular advantages of the new system include the immediate linkage of all specimens for each patient and an easy recall and display of results in the laboratories and on the wards

Isoniazid activity is terminated by bacterial persistence [2]

[No abstract available]Lette