Initial Plant Growth in Sand Mine Spoil Amended with Organic Materials

Sand dunes in the Great Lakes Basin are ecologically and economically important. One economic value of sand dunes is sand mining. However, sand mining activities reduce soil quality which represents an impediment to reclamation efforts. Soil quality improvements followed by revegetation may be necessary for successful reclamation. This study evaluates the germination and initial growth of two legume species, wild lupine (Lupinus perennis) and Illinois bundleflower (Desmanthus illinoensis), and two warm-season grass species, Indian grass (Sorghastrum nutans) and little bluestem (Schizachyrium scoparium), in the presence of three soil amendments (coco peat, municipal sewage sludge, and sphagnum peat moss) added to spoil from a local sand mine. Species were grown in pots and propagated under greenhouse conditions. Our results indicate that lupine and Illinois bundleflower exhibited the greatest germination and growth and are recommended as potential candidates for the reclamation of sand mines. Indian grass and little bluestem exhibited low rates of germination and growth. Among spoil amendments, we recommend coco peat based on germination and root, shoot, and total biomass results. Peat moss also exhibited increased rates of germination and growth. We did not observe any germination success for seeds sown in sewage sludge amended spoil. The lack of germination in the sewage sludge amendment may be due to a combination of unfavorable abiotic and biotic soil conditions such as electrical conductivity, pH, or soil microbial activity. Our results are helpful in determining which species and amendment combinations are useful candidates for revegetating former sand mines or similar habitats

Kukutali Preserve: co-managing the first tribal-state park in Washington

Kukutali Preserve (Kukutali) is a unit of Deception Pass State Park and located within the Swinomish Indian Reservation near LaConner, Washington, as established by treaty in 1855. The upland area included in Kukutali was sold out of tribal ownership in 1928 and spent most of the next 82 years in private title with minimal development; as a result, Kukutali contains examples of rare, pristine habitat. Parks had long been interested in acquiring the property to preserve, and provide public recreational access to, its diverse ecosystems. SITC also had a long-standing wish to reacquire the property and restore access to its cultural and natural resources for Tribal citizens. In 2010, Parks and SITC entered into a historic co-management agreement to jointly acquire Kiket Island and an adjacent nine acre parcel on Fidalgo Island. SITC and Parks agreed to work in partnership to develop, operate, and maintain the islands and associated tidelands as a unit of Deception Pass State Park with the stipulation that the lands remain available for “limited public access consistent with protection of the resources and ecology of Kiket Island.” Parks and SITC co-own the upland portion of Kukutali, with SITC retaining sole ownership of the adjacent tidelands, and co-manage Kukutali via a board composed of three SITC representatives and three Parks representatives using consensus decision making. Kukutali is managed with two main goals: to protect the unique ecosystems and enhance the biodiversity on and around Kiket Island, and to provide the general public with an opportunity to learn about the natural ecosystems and take a part in the stewardship of these lands. A conservation management and restoration plan, developed by SITC and approved by the management board, provides guidance in this effort. Maintenance and operational costs are shared equally by the co-owners

Disproportionate School Disciplinary Responses: An Exploration of Prisonization and Minority Threat Hypothesis Among Black, Hispanic, and Native American Students

This research tests two potential explanations of school disciplinary responses: minority threat hypothesis and prisonization of schools. Data from the Arizona Safe and Drug-Free Schools (SDFS) survey and Arizona Youth Survey (AYS) are analyzed using ordinary least squares (OLS) regressions. Findings demonstrate that the percentage of Black, Hispanic, and Native American students was not associated with exclusionary responses to school misconduct, but was linked to decreases in mild and restorative disciplinary practices. Findings support the hypothesis that minority threat reduces access to mild and restorative disciplinary responses. Although, further research is needed on the roles of mental health professionals and counselors in school disciplinary procedures to better guide policy and school administrator expectations

Evaluation of surface water resources from machine-processing of ERTS multispectral data

The surface water resources of a large metropolitan area, Marion County (Indianapolis), Indiana, are studied in order to assess the potential value of ERTS spectral analysis to water resources problems. The results of the research indicate that all surface water bodies over 0.5 ha were identified accurately from ERTS multispectral analysis. Five distinct classes of water were identified and correlated with parameters which included: degree of water siltiness; depth of water; presence of macro and micro biotic forms in the water; and presence of various chemical concentrations in the water. The machine processing of ERTS spectral data used alone or in conjunction with conventional sources of hydrological information can lead to the monitoring of area of surface water bodies; estimated volume of selected surface water bodies; differences in degree of silt and clay suspended in water and degree of water eutrophication related to chemical concentrations

Soil conductivity study and implications for fish and farming compatibility in the Swinomish agricultural area

The Swinomish agricultural area, along the Swinomish Channel, is part of the Skagit River Delta, a major agrarian region in Puget Sound. Historically a complex system of tidal channels serving as salmon habitat, the tidelands have since been diked and drained. In 2005, the Swinomish Indian Tribal Community (SITC) began restoration to demonstrate compatibility of fish habitat and agriculture. Muted tidal regulators (MTRs) operated to optimize tidal inundation and fish passage, replaced traditional tidegates, and prompted a study to evaluate soil conductivity impacts on agriculture. Objectives included electromagnetic (EM) surveying of soil conductivity, qualitatively assessing EM results utilizing two additional methods, and assessing local crop suitability and restoration effectiveness for fish habitat and agriculture compatibility. All methods showed low conductivity throughout the majority of the area with isolated elevated levels adjacent to drainage ditches, the main Channel dike, and in low-lying depressional areas. Designed for periodic inundation, a restoration area yielded some of the highest conductivity levels. Using conversion formulas applicable to each conductivity measurement method, conductivity values were converted to salinity classes to evaluate potential adverse crop effects. The areas described above ranged from primarily ‘slightly saline’ and ‘moderately saline’ (many crop yields restricted), to ‘strongly saline’, with marginal ‘very strongly saline’ areas along the main dike and in the restoration area. However, the vast majority of the agricultural area could be categorized as ‘non-saline’, with negligible crop effects. These results indicate that the restoration designed to enhance fish habitat resulted in limited salinity intrusion to the adjacent cropland. This study, along with concurrent projects in the Swinomish agricultural area, suggests that fish and farming, two economically and culturally significant symbols of the region, may not only survive, but thrive in the same space

Framing Scientific Analyses for Risk Management of Environmental Hazards by Communities: Case Studies with Seafood Safety Issues

Risk management provides a context for addressing environmental health hazards. Critical to this approach is the identification of key opportunities for participation. We applied a framework based on the National Research Council’s (NRC) analytic–deliberative risk management dialogue model that illustrates two main iterative processes: informing and framing. The informing process involves conveying information from analyses of risk issues, often scientific, to all parties so they can participate in deliberation. In the framing process, ideas and concerns from stakeholder deliberations help determine what and how scientific analyses will be carried out. There are few activities through which affected parties can convey their ideas from deliberative processes for framing scientific analyses. The absence of participation results in one-way communication. The analytic–deliberative dialogue, as envisioned by the NRC and promoted by the National Institute of Environmental Health Sciences (NIEHS), underscores the importance of two-way communication. In this article we present case studies of three groups—an Asian and Pacific Islander community coalition and two Native American Tribes—active in framing scientific analyses of health risks related to contaminated seafood. Contacts with these organizations were established or enhanced through a regional NIEHS town meeting. The reasons for concern, participation, approaches, and funding sources were different for each group. Benefits from their activities include increased community involvement and ownership, better focusing of analytical processes, and improved accuracy and appropriateness of risk management. These examples present a spectrum of options for increasing community involvement in framing analyses and highlight the need for increased support of such activities

Influence of ceramide on lipid domain stability studied with small-angle neutron scattering: The role of acyl chain length and unsaturation

Ceramides and diacylglycerols are groups of lipids capable of nucleating and stabilizing ordered lipid domains, structures that have been implicated in a range of biological processes. Previous studies have used fluorescence reporter molecules to explore the influence of ceramide acyl chain structure on sphingolipid-rich ordered phases. Here, we use small-angle neutron scattering (SANS) to examine the ability of ceramides and diacylglycerols to promote lipid domain formation in the well-characterized domain- forming mixture DPPC/DOPC/cholesterol. SANS is a powerful, probe-free technique for interrogating membrane heterogeneity, as it is differentially sensitive to hydrogen\u27s stable isotopes protium and deuterium. Specifcally, neutron contrast is generated through selective deuteration of lipid species, thus enabling the detection of nanoscopic domains enriched in deuterated saturated lipids dispersed in a matrix of protiated un- saturated lipids. Using large unilamellar vesicles, we found that upon replacing 10 mol % DPPC with either C16:0 or C18:0 ceramide, or 16:0 diacylglycerol (dag), lipid domains persisted to higher temperatures. However, when DPPC was replaced with short chain (C6:0 or C12:0) or very long chain (C24:0) ceramides, or ceramides with unsaturated acyl chains of any length (C6:1(3), C6:1(5), C18:1, and C24:1), as well as C18:1-dag, lipid domains were destabilized, melting at lower temperatures than those in the DPPC/DOPC/cholesterol system. These results show how ceramide acyl chain length and unsaturation influence lipid domains, and have implications for how cell membranes might modify their function through the generation of different ceramide species

caCORRECT2: Improving the accuracy and reliability of microarray data in the presence of artifacts

© 2011 Moffitt et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.DOI: 10.1186/1471-2105-12-383Background. In previous work, we reported the development of caCORRECT, a novel microarray quality control system built to identify and correct spatial artifacts commonly found on Affymetrix arrays. We have made recent improvements to caCORRECT, including the development of a model-based data-replacement strategy and integration with typical microarray workflows via caCORRECT's web portal and caBIG grid services. In this report, we demonstrate that caCORRECT improves the reproducibility and reliability of experimental results across several common Affymetrix microarray platforms. caCORRECT represents an advance over state-of-art quality control methods such as Harshlighting, and acts to improve gene expression calculation techniques such as PLIER, RMA and MAS5.0, because it incorporates spatial information into outlier detection as well as outlier information into probe normalization. The ability of caCORRECT to recover accurate gene expressions from low quality probe intensity data is assessed using a combination of real and synthetic artifacts with PCR follow-up confirmation and the affycomp spike in data. The caCORRECT tool can be accessed at the website: http://cacorrect.bme.gatech.edu webcite. Results. We demonstrate that (1) caCORRECT's artifact-aware normalization avoids the undesirable global data warping that happens when any damaged chips are processed without caCORRECT; (2) When used upstream of RMA, PLIER, or MAS5.0, the data imputation of caCORRECT generally improves the accuracy of microarray gene expression in the presence of artifacts more than using Harshlighting or not using any quality control; (3) Biomarkers selected from artifactual microarray data which have undergone the quality control procedures of caCORRECT are more likely to be reliable, as shown by both spike in and PCR validation experiments. Finally, we present a case study of the use of caCORRECT to reliably identify biomarkers for renal cell carcinoma, yielding two diagnostic biomarkers with potential clinical utility, PRKAB1 and NNMT. Conclusions. caCORRECT is shown to improve the accuracy of gene expression, and the reproducibility of experimental results in clinical application. This study suggests that caCORRECT will be useful to clean up possible artifacts in new as well as archived microarray data