Ekspresi Phosphatase Regenerating Liver-3 dan E-Cadherin pada Kanker Serviks Stadium Awal dan Lanjut Lokoregional

Tujuan: untuk menyelidiki hubungan antara ekspresi PRL-3 dan E-Cadherin dengan stadium, jenis histopatologi, dan derajat diferensiasi kanker serviks. Metode: penelitian ini merupakan studi analitik dengan pendekatan potong lintang di beberapa rumah sakit pendidikan Bagian Obstetri dan Ginekologi, Makassar, mulai Januari hingga Juni 2011. Ekspresi PRL-3 dan E-Cadherin diperiksa dengan pengecatan imunohistokimia pada 16 kanker serviks stadium awal dan 15 stadium lanjut lokoregional. Hasil: ekspresi PRL-3 tidak berbeda bermakna antara kanker serviks stadium awal dengan stadium lanjut lokoregional, antara berbagai jenis histopatologik dan derajat diferensiasi (p>0,05). Ekspresi E-Cadherin juga menunjukkan tidak ada perbedaan bermakna antara berbagai stadium, jenis histopatologi, dan derajat diferensiasi (p>0,05). Tidak didapatkan korelasi bermakna antara ekspresi PRL-3 dengan E-Cadherin pada penelitian ini. Kesimpulan: tidak ada hubungan antara ekspresi PRL-3 dan E-Cadherin dengan stadium, jenis histopatologi, dan derajat diferensiasi kanker serviks

DISTRIBUTION PATTERNS OF ANGIOGENESIS IN COLORECTAL CANCER: STUDY PROTEIN EXPRESSION OF ENDOGLIN (CD105)

ABSTRACT Introduction: Angiogenesis plays an important role in the growth and metastasis of colorectal carcinoma, which is currently one of the targets of cancer therapy. It has been reported that the Endoglin (CD105) involved in angiogenesis and is a powerful marker for angiogenesis in colorectal carcinoma. Level Quantitative angiogenesis in peritumor and an intratumor area is important to know because it is closely related to the micro-environmental factors that influence the occurrence of cancer angiogenesis. The goal of this study to analyze the distribution pattern of angiogenesis in colorectal cancer by comparing the distribution of angiogenesis in peritumor and intratumor areas between well, moderate and poorly differentiated colorectal carcinoma, and between metastasis and non-metastatic colorectal cancer. Methods: This study analyzed fifty samples of resected adenocarcinoma colorectal. Angiogenesis was assessed by the immunohistochemical method using a primary monoclonal antibody Endoglin (CD105). Positive expression of CD105 was assessed through the CD105 protein expression in neovascular endothelial cells, while the distribution pat- tern of angiogenesis assessed by counting the positive expression of CD105 protein in hot spots by using the MVD (microvessel density) in the peritumor and intratumor areas and then performed statistical analysis. Results: There is a significant difference between the quantitative level of an- giogenesis in peritumor and intratumor areas of well (P<0.01), moderate (P<0.01) and poorly (<0.05) differentiated adenocarcinoma. The significant difference between the quantitative levels of angio- genesis in peritumor and intratumor areas of non-metastatic colorectal cancer (P<0.01) and lymph node metastases (<0.05) was found, but not in colorectal cancer with liver metastasis. Conclu- sion: Angiogenesis pattern is more concentrated in peritumor compared to intratumor areas. This showed the role of stromal cells in-angiogenesis. There is significant expression between angio- genesis in peritumor and intratumor areas. Keywords: Angiogenesis, Colorectal cancer, Endoglin (CD105

Clinical and Pathological Characteristics of High Grade Colorectal Adenocarcinoma at Hasanuddin University Hospital, Indonesia, 2015-2020

Colorectal cancer is one of the most common cancers and the fourth leading cause of cancer-related death in the world. According to the type of histopathology, more than 90% of colorectal carcinomas are adenocarcinomas which are grouped into low grade and high grade based on their grading. Identification of characteristic groupings based on grading is still rarely done in Indonesia, especially in Makassar. This study aims to determine the clinical and pathological characteristics of high grade colorectal adenocarcinoma at Hasanuddin University Hospital, Makassar from January 2015 to December 2020. This study used a descriptive method. The sample used was a sample that was diagnosed histopathologically as high grade colorectal adenocarcinoma and met the inclusion criteria then selected by total sampling and also used data from medical records. The distribution of data in this study is classified based on age, gender, tumor location, clinical symptoms, and tumor stage. Descriptive statistical calculations were performed using SPSS 20 for Windows software. There were 98 samples, most of which were at the age of ≥50 years, as many as 32 samples (32.7%) with man as many as 60 samples (61.2%). The most common location was in the rectum as many as 38 samples (38.8%) with the most clinical symptoms were bowel disorders as many as 68 samples (69.4%) and stage 3 was the most cases with 35 samples (35.7%). &nbsp

Clinicopathological Profile of Colorectal Cancer Patients in Anatomical Pathology Department of Hasanuddin University Makassar

Colorectal cancer (CRC) is the third most common cancer in men, the second in women, and it’s the second for leading cause of cancer death worldwide, with more than 1.2 million new cancer cases and 608,700 deaths. The World Health Organization (WHO) in 2018, explained that globally there were 1.80 million new cases of colorectal cancer diagnosed and 862,000 patients died of colorectal cancer. This study aims to define the epidemiological and clinicopathological characteristics of CRC with metastasize in Makassar. Retrospective study was performed used cross-sectional design included all patients diagnosed with colorectal adenocarcinoma with metastasize at Anatomical Pathology Laboratory Dr. Wahidin Sudirohusodo, Faculty of Medicine Hasanuddin University and Makassar Pathology Diagnostic Center, from January 2016 to December 2021. A total of 60 patients were included in this study, 45% were females and 55% were males. Samples with the age category <50 years were 13 samples (21.7%) and the age category > 50 years were 47 samples (78.3%). Based on the location of the tumor, the location of tumor in the right colon were 24 samples (40.0%), in the left colon were 14 samples (23.3%), and in the rectum were 22 (36.7%) samples. The low-grade colorectal adenocarcinoma group were consisted of 31 samples (51.7%) and 29 samples (48.3%) of the high-grade. Samples with positive lymphovascular invasion were 17 samples (51.7%) and 43 samples (71.7%) were negative.The depth of invasion were 28 samples (46.7%) at muscularis externa and 32 samples (53.3%) at serosa.   Colorectal adenocarcinoma is more frequent at the age more than 50 years old and right colon is the most affected sub site. The mean age at diagnosis was 57.2 years with male predominance. Most patients had low grade tumor, no lymphovascular invasion findings at microscopic and the depth of invasion reach the serosa. Lymph nodes is the most common metastatic site

Comparison of The Means of Argyrophilic Nucleolar Organizer Region (mAgNOR) Pre- and Post-Therapy in Nasopharyngeal Carcinoma Patients at Wahidin Sudirohusodo General Hospital Makassar

BACKGROUND: Nasopharyngeal carcinoma (NPC) is malignant tumor growing in nasopharynx with a predilection in fossa Rossenmuller and nasopharyngeal roof. This research aimed to prove whether the means of argyrophilic nucleolar organizer region (mAgNOR) can predict the success of treatment in nasopharyngeal carcinoma patients.METHODS: We used diagnostic test method with longitudinal design and purposive sampling technique. Endoscopic biopsy examination was performed on 15 nasopharyngeal carcinoma patients before and after therapy, 13 patients underwent chemotherapy and other two underwent chemoradiotherapy. Tumor tissues were stained and AgNOR was calculated.RESULTS: Based on the tumor stage, sample characteristic showed 3 patients (20%) were in stage II, 3 patients (20%) in stage III, and 9 patients (60%) in stage IV, with pre- and post-therapy mAgNOR were 1.610±0.988 and 1.000±0.000, respectively in stage II, 1.100±0.092 and 1.000±0.000, respectively in stage III, 1.226±0.265 and 1.107±0.164, respectively in stage IV patients. Based on histopathology type, 4 patients (26.7%) had non keratinizing squamous cell carcinoma with pre- and post-therapy mAgNOR were 1.117±0.134 and 1.060±0.120, respectively, while 11 patients (73.3%) had undifferentiated squamous cell carcinoma with pre- and post-therapy mAgNOR were 1.335±0.528 and 1.065±0.146, respectively. Overall the pre-therapy were significantly higher than post-therapy mAgNOR. In subgroups there are significant differences in stage IV and type 3.CONCLUSION: The values of AgNOR were decreased in all NPC stages and significantly decreased in undifferentiated squamous cell carcinoma. AgNOR can be used to predict the successfulness of therapy in NPC.KEYWORDS: nasopharyngeal carcinoma, therapy, proliferation, mAgNO

Tumour-Infiltrating Lymphocytes in Colorectal Adenocarcinoma

Several studies have indicated that lymphocytes found in tumour area, known as TILs (tumour-infiltrating lymphocytes), can provide prognostic information in colorectal cancer (CRC). The purpose of this study was to evaluate degree of stromal TILs  according to histological grading of CRC. This study used  a cross sectional design method by taking random samples of data and formalin-fixed paraffin-embedded of colorectal adenocarcinoma patients treated in Dr. Wahidin Sudirohusodo Hospital and Hasanuddin University Hospital in Makassar during the period of 2014-2016. The histological grading of each sample was reviewed and  TILs were assessed according to recommendation of the International TILs Working Group, 2014 on hematoxylin-eosin (H-E)-stained sections. Based on histological grading it was found that from 98 samples, 23 samples (23.5%) were well-differentiated, 66 samples (67.3%) were moderately differentiated, and 6 samples were (9.2%) poorly differentiated. While based on degree of TILs found that low- and moderate-score were 38 (38.8%) and 42 (42.9%) samples respectively, and high-score were 18 (18.4%) samples. Data were analysed using the Chi-square test and a p value of 0.865 (p > 0.05) was obtained and therefore it was concluded that there was no significant difference of degree of TILs according to histological grading of colorectal adenocarcinoma in this study

Programmed Death – Ligand 1 Expression in Malignancy of Thyroid Follicular Epithelial Cell Origin

Several previous studies reported the fact that expression of Programmed Death – Ligan 1 (PD-L1) in various types of histopathology of thyroid cancer showed varied results and had predictive value and prognosis that were expected to be targeted for anti PD-1/PD-L1 immunotherapy. The aim of this study was to evaluate comparison expression of PD-L1 one to each other group between papillary thyroid carcinoma (PTC), follicular thyroid carcinoma (FTC), poorly differentiated carcinoma (PDTC) and anaplastic thyroid carcinoma (ATC). This study was an observational analytic with a cross sectional design using paraffin block samples from three anatomic pathology laboratories in Makassar during the periode of July 2015 – February 2019. PD-L1 expression was evaluated using Rabbit Monoclonal Antibody (28-8) and data were analysed using The Mann – Whitney Test. There was a significant difference of PD-L1 expression score between PTC with PDTC (p = 0,045 ; p < 0,05) and ATC (p = 0,046 ; p < 0,05), whereas there was no significant difference of PD-L1 expression score between PTC with FTC (p = 0,371 ; p > 0,05), between FTC with PDTC (p = 0,147 ; p > 0,05) and ATC (p = 0,069 ; p > 0,05), also between PDTC with ATC (p = 0,483 ; p > 0,05). But overall, PD-L1 expression showed higher expression in a subset of advanced thyroid cancers such as poorly differentiated thyroid carcinoma and anaplastic thyroid carcinoma

Correlation of Baseline BCL-2 mRNA Expression and Clinical Response to Neoadjuvant Chemotherapy in Breast Cancer

Background: Impairment of apoptosis is a hallmark of cancer. Tumor resistance to apoptosis usually caused by deregulation of the expression of BCL-2 family protein or mutation of the tumor suppressor gene p53. Over expression of Bcl-2 is commonly found in various types of cancer including breast cancer. Studies mentioned that analysis of Bcl-2 might predict response to selected endocrine and chemotherapies. Purpose: Evaluation of correlation of BCL-2 mRNA expression and clinical response to neoadjuvant chemotherapy in breast cancer patients. Method: longitudinal study, 30 subjects of breast cancer tissue samples prechemotherapy using cyclophosphamide-adriamycin-5FU regiment. Detection of mRNA expression of BCL-2 using qRT-PCR techniques

Correlation of FOXP3 Expression in Placenta as Regulatory T Cells Marker with Maternal HBeAg Serum and Intrauterine Transmission Status

Hepatitis B is an infectious disease caused by the Hepatitis B Virus (HBV) with a risk of transplacental (mother-to-child) transmission which is responsible for the failure of chil

Relationship of Survivin Expression with Histopathological Grade and Events of Metastasis to Omentum in Serous Carcinoma of Ovary

Ovarian cancer is the third most common cancer in Indonesia after cervical and breast cancer with the increasing incidence and mortality. The purpose of this study was to determine the relationship of survivin expression with the degree of histopathology and the incidence of metastasis to the omentum using immunohistochemical examinations. This is a cross-sectional study using 72 paraffine blocks of serous carcinoma of ovary without and with metastasis to omentum in Makassar, Indonesia during 2015-2018. Survivin was positive in tumor cells with a different degree based on the grade of histopathology and the incidence of metastases to the omentum. There was a significant difference between survivin expression with the degree of histopathology and the incidence of metastases to the omentum in serous carcinoma of ovary (p = 0.000)