Influence of silver-ion-containing pharmacotherapeutic system for repair of anterior abdominal wall on connective tissue formation in experiment

In modern medicine new pharmacotherapeutic systems significantly reducing the risk of complications are being actively searched for. The study undertaken was aimed at studying one of such systems consisting of a prosthesis coated with silver ions. The article notes that use of FCS contributes to the acceleration of reparative processes, earlier resolution of inflammation and stimulation of collagenesis both under sterile conditions and under microbial condition

The use of immobilized form of benzalkonium chloride and metronidazole in the treatment of purulent wounds

The purpose of the study is to investigate the wound-healing effect of the immobilized form of benzalkonium chloride and metronidazole based on the sodium salt of carboxymethylcellulose (Na-CMC) or polyethylene oxide (PEO) in the treatment of an experimental purulent wound.Materials and methods. The following compositions were studied, %. Combination 1: benzalkonium chloride – 0.02; Metronidazole – 1,0; Sodium carboxymethylcellulose (NaCMC) – 4,0; Purified water – up to 100,0. Combination 2: benzalkonium chloride – 0,02; Metronidazole – 1,0; Polyethylene oxide, m. 400 – 80.0; Polyethylene oxide, m. 1500 – 20,0. The experiment was performed on 180 male Wistar rats, which were divided into three statistically homogeneous groups of 60 animals in each, a purulent wound was modeled in all experimental rats using the method of P.I. Tolstykh. In the control group local treatment of the wound was performed with the help of “Levomekol” ointment, in two experimental groups, the combination was treated with 1 and 2.The course evaluation of the wound process was carried out using planimetric, microbiological and histological methods of investigation. The data were recorded and the animals were eliminated from the experiment on the 1st, 3rd, 5th, 8th, 10th and 15th days.Results. The data of the microbiological study confirmed the high efficiency of the combinations developed by the authors in relation to standard test strains of pathogen microorganisms. As a result of the planimetric study, a significant decrease in the area of wounds in the experimental group 1 was found compared to the ontrol group, which indicates a more effective course of the healing process in experimental group 1. The same process was noted in the experimental group 2, starting from the 5th day of observation relative to the Control. The use of combinations developed by the authors in the treatment of the purulent-inflammatory process of the subcutaneous layer promotes the rapid reduction of microbial contamination of wounds compared to “Levomekol” ointment. After the histological examination, it was noted that the processes of regeneration and epithelization proceeded better in the experimental groups relative to the control.Conclusion. The immobilized form of benzalkonium chloride and metronidazole in polyethylene oxide gel (combination 2) has the highest antimicrobial activity, in comparison with the rest of the preparations, and is statistically significant, in comparison with the control group. It also reduces the wound area and accelerates the epithelization period. At the same time, there are no significant differences between combination 1 and ointment “Levomecol” in the zones of growth retardation of microorganisms. Combination 1 shows the highest activity in the 1st day of the wound process, so it should be recommended for the treatment of purulent wounds in the first 3 days of the first phase of the wound process. Combination 2 should be recommended starting from the 3rd day of treatment, when the highest healing rate has been noted when applied in the experimental group 2

UMAOH calcium phosphate coatings designed for drug delivery: vancomycin, 5-fluorouracil, interferon α-2b case

Drug delivery systems based on calcium phosphate (CaP) coatings have been recently recognized as beneficial drug delivery systems in complex cases of bone diseases for admission of drugs in the localized area, simultaneously inducing osteoinduction because of the bioavailable Ca and P ions. However, micro-arc oxidation (MAO) deposition of CaP does not allow for the formation of a coating with sufficient interconnected porosity for drug delivery purposes. Here, we report on the method to deposit CaP-based coatings using a new hybrid ultrasound-assisted MAO (UMAOH) method for deposition of coatings for drug delivery that could carry various types of drugs, such as cytostatic, antibacterial, or immunomodulatory compositions. Application of UMAOH resulted in coatings with an Ra roughness equal to 3.5 mu m, a thickness of 50-55 mu m, and a combination of high values of internal and surface porosity, 39 and 28%, respectively. The coating is represented by the monetite phase that is distributed in the matrix of amorphous CaP. Optimal conditions of coating deposition have been determined and used for drug delivery by impregnation with Vancomycin, 5-Fluorouracil, and Interferon-alpha-2b. Cytotoxicity and antimicrobial activity of the manufactured drug-carrying coatings have been studied using the three different cell lines and methicillin-resistant S. aureus

Live-Cell STED Microscopy with Genetically Encoded Biosensor

Of the various super-resolution techniques, stimulated emission depletion (STED) microscopy achieves the best temporal resolution at high spatial resolution, enabling live-cell imaging beyond the diffraction limit. However, STED and most other super-resolution imaging methods utilize a particular type of information extractable from the raw data, namely the positions of fluorophores. To expand on the use of super-resolution techniques, we report here the live-cell STED microscopy of a dynamic biosensor. Using the fluorescent H₂O₂ sensor HyPer2 for subdiffraction imaging, we were able not only to image filaments with superior resolution by localizing emission but also to trace H₂O₂ produced within living cell by monitoring brightness of the probe. STED microscopy of HyPer2 demonstrates potential utility of FP-based biosensors for super-resolution experiments in situ and in vivo