15 research outputs found
Numerical Modelling of Optical Trapping
Optical trapping is a widely used technique, with many important applications
in biology and metrology. Complete modelling of trapping requires calculation
of optical forces, primarily a scattering problem, and non-optical forces. The
T-matrix method is used to calculate forces acting on spheroidal and
cylindrical particles.Comment: 4 pages, 4 figure
Calculation and optical measurement of laser trapping forces on non-spherical particles
Optical trapping, where microscopic particles are trapped and manipulated by
light is a powerful and widespread technique, with the single-beam gradient
trap (also known as optical tweezers) in use for a large number of biological
and other applications.
The forces and torques acting on a trapped particle result from the transfer
of momentum and angular momentum from the trapping beam to the particle.
Despite the apparent simplicity of a laser trap, with a single particle in a
single beam, exact calculation of the optical forces and torques acting on
particles is difficult. Calculations can be performed using approximate
methods, but are only applicable within their ranges of validity, such as for
particles much larger than, or much smaller than, the trapping wavelength, and
for spherical isotropic particles.
This leaves unfortunate gaps, since wavelength-scale particles are of great
practical interest because they are readily and strongly trapped and are used
to probe interesting microscopic and macroscopic phenomena, and non-spherical
or anisotropic particles, biological, crystalline, or other, due to their
frequent occurance in nature, and the possibility of rotating such objects or
controlling or sensing their orientation.
The systematic application of electromagnetic scattering theory can provide a
general theory of laser trapping, and render results missing from existing
theory. We present here calculations of force and torque on a trapped particle
obtained from this theory and discuss the possible applications, including the
optical measurement of the force and torque.Comment: 10 pages, 5 figure
Calculation of the T-matrix: general considerations and application of the point-matching method
The T-matrix method is widely used for the calculation of scattering by
particles of sizes on the order of the illuminating wavelength. Although the
extended boundary condition method (EBCM) is the most commonly used technique
for calculating the T-matrix, a variety of methods can be used.
We consider some general principles of calculating T-matrices, and apply the
point-matching method to calculate the T-matrix for particles devoid of
symmetry. This method avoids the time-consuming surface integrals required by
the EBCM.Comment: 10 pages. 2 figures, 1 tabl
Multipole expansion of strongly focussed laser beams
Multipole expansion of an incident radiation field - that is, representation
of the fields as sums of vector spherical wavefunctions - is essential for
theoretical light scattering methods such as the T-matrix method and
generalised Lorenz-Mie theory (GLMT). In general, it is theoretically
straightforward to find a vector spherical wavefunction representation of an
arbitrary radiation field. For example, a simple formula results in the useful
case of an incident plane wave. Laser beams present some difficulties. These
problems are not a result of any deficiency in the basic process of spherical
wavefunction expansion, but are due to the fact that laser beams, in their
standard representations, are not radiation fields, but only approximations of
radiation fields. This results from the standard laser beam representations
being solutions to the paraxial scalar wave equation. We present an efficient
method for determining the multipole representation of an arbitrary focussed
beam.Comment: 13 pages, 7 figure
Optical application and measurement of torque on microparticles of isotropic nonabsorbing material
We show how it is possible to controllably rotate or align microscopic
particles of isotropic nonabsorbing material in a TEM00 Gaussian beam trap,
with simultaneous measurement of the applied torque using purely optical means.
This is a simple and general method of rotation, requiring only that the
particle is elongated along one direction. Thus, this method can be used to
rotate or align a wide range of naturally occurring particles. The ability to
measure the applied torque enables the use of this method as a quantitative
tool--the rotational equivalent of optical tweezers based force measurement. As
well as being of particular value for the rotation of biological specimens,
this method is also suitable for the development of optically-driven
micromachines.Comment: 8 pages, 6 figure
Endocannabinoid System Participates in Neuron-Glial Networks Adaptation to Modeled Ischemia Factors in vitro
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Лікування хворих з пошкодженням нирок при політравмі
Around 4.5 million of people suffer from traumas in Ukraine. Renal injuries in case of polytrauma are observed in 26.4% of cases and occupy second or third place among injuries of organs of abdominal cavity and retroperitoneal space. To study and prove the influence of the lymph stimulation therapy on functional condition of kidneys and vital organs and systems at suffered people with renal injuries in case of polytrauma we observed 218 patients, who were divided into two groups.
In the first main group the lymph stimulation therapy was conducted and the patients of the second group took traditional therapy. The main clinical and biochemical indicators of blood and urine were studied by laboratory methods; ultrasound diagnostics and CT scan of kidneys and organs of abdominal cavity were done.
It was proved that in patients, who received the lymph stimulation therapy, indicators of creatinine and indicators of other organs and systems normalized at 7 days earlier than in patients, who receive traditional therapy.В Украине вследствие травм страдают до 4,5 млн. человек. Повреждения почек при политравме наблюдаются у 26,4% случав и занимают 2–3 места среди повреждений органов брюшной полости и забрюшинного пространства. С целью обоснования и изучения влияния лимфостимулирующей терапии на функциональное состояние почек и жизненноважных органов и систем у потерпевших с повреждением почек при политравме наблюдали 218 больных, которые были разделены на две группи. В 1-й основной группе проводилась лимфостимулирующая терапия, а больные 2-й группы получали традиционную терапию. Лабораторными методами изучены основные клинико-биохимические показатели крови и мочи, УЗИ та КТ почек и органов брюшной полости.
Доказано, что у больных, которые получали лимфостимулирующую терапию, показатели креатинина и показатели других органов и систем нормализуются на 7 суток раньше, чем при традиционной терапии.В Україні внаслідок травм страждають до 4,5 млн. чоловік. Пошкодження нирок при політравмі спостерігаються у 26,4% випадків і займають 2-3 місця серед пошкоджень органів черевної порожнини і заочеревинного простору. З метою обґрунтування і вивчення впливу лімфостимулювальним терапії на функціональний стан нирок і життєвоважливих органів і систем у потерпілих з ушкодженням нирок при політравмі спостерігали 218 хворих, які були розділені на дві группи. У 1-й основній групі проводилася лімфостимулювальним терапія, а хворі 2-ї групи отримували традиційну терапію. Лабораторними методами вивчені основні клініко-біохімічні показники крові і сечі, УЗД та КТ нирок і органів черевної порожнини.
Доведено, що у хворих, які отримували лімфостимулювальним терапію, показники креатиніну та показники інших органів і систем нормалізуються на 7 діб раніше, ніж при традиційній терапії
FEATURES OF THE ELECTRICAL CONDUCTIVITY AND RESISTANCE OF CEMENT FOR FIXATION OF NON-REMOVABLE PROSTHETIC
Our studies show different resistance and consequently different electrical conductivity of dry and wet samples of studied cements. Particular attention is drawn to the fact of reducing the relative value of the resistance of cements to fix after humidification. It can be evidence of higher hydrophobicity of the samples which have greater resistance after humidification. Thus, it is possible to recommend the use for fixing sintered structures made with reference bits according to our proposed method “Tsemion” and polycarboxylate cement. Since these cements have high electrical resistance and a corresponding lower electric conductivity, there is a slight decrease in the resistance after wetting the samples
Immunogenic cell death induced by a new photodynamic therapy based on photosens and photodithazine
Background: Anti-cancer therapy is more successful when it can also induce an immunogenic form of cancer cell death (ICD). Therefore, when developing new treatment strategies, it is extremely important to choose methods that induce ICD and thereby activate anti-Tumor immune response leading to the most effective destruction of tumor cells. The aim of this work was to analyze whether the clinically widely used photosensitizers, photosens (PS) and photodithazine (PD), can induce ICD when used in photodynamic therapy (PDT). Methods: Cell death in murine glioma GL261 or fibrosarcoma MCA205 cells was induced by PS-or PD-PDT and cell death was analyzed by MTT or flow cytometry. Intracellular distribution of PS and PD was studied by using the laser scanning microscope. Calreticulin exposure and HMGB1 and ATP release were detected by flow cytometry, ELISA and luminescence assay, respectively. Immunogenicity in vitro was analyzed by co-culturing of dying cancer cells with bone-marrow derived dendritic cells (BMDCs) and rate of phagocytosis and maturation (CD11c+CD86+, CD11c+CD40+) of BMDCs and production of IL-6 in the supernatant were measured. In vivo immunogenicity was analyzed in mouse tumor prophylactic vaccination model. Results: We determined the optimal concentrations of the photosensitizers and found that at a light dose of 20 J/cm2 (\u3bbex 615-635 nm) both PS and PD efficiently induced cell death in glioma GL261 and fibrosarcoma MCA205 cells. We demonstrate that PS localized predominantly in the lysosomes and that the cell death induced by PS-PDT was inhibited by zVAD-fmk (apoptosis inhibitor) and by ferrostatin-1 and DFO (ferroptosis inhibitors), but not by the necroptosis inhibitor necrostatin-1 s. By contrast, PD accumulated in the endoplasmic reticulum and Golgi apparatus, and the cell death induced by PD-PDT was inhibited only by z-VAD-fmk. Dying cancer cells induced by PS-PDT or PD-PDT emit calreticulin, HMGB1 and ATP and they were efficiently engulfed by BMDCs, which then matured, became activated and produced IL-6. Using dying cancer cells induced by PS-PDT or PD-PDT, we demonstrate the efficient vaccination potential of ICD in vivo. Conclusions: Altogether, these results identify PS and PD as novel ICD inducers that could be effectively combined with PDT in cancer therapy