In Vitro Model of Vascularized Bone: Synergizing Vascular Development and Osteogenesis

Tissue engineering provides unique opportunities for regenerating diseased or damaged tissues using cells obtained from tissue biopsies. Tissue engineered grafts can also be used as high fidelity models to probe cellular and molecular interactions underlying developmental processes. In this study, we co-cultured human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (MSCs) under various environmental conditions to elicit synergistic interactions leading to the colocalized development of capillary-like and bone-like tissues. Cells were encapsulated at the 1∶1 ratio in fibrin gel to screen compositions of endothelial growth medium (EGM) and osteogenic medium (OM). It was determined that, to form both tissues, co-cultures should first be supplied with EGM followed by a 1∶1 cocktail of the two media types containing bone morphogenetic protein-2. Subsequent studies of HUVECs and MSCs cultured in decellularized, trabecular bone scaffolds for 6 weeks assessed the effects on tissue construct of both temporal variations in growth-factor availability and addition of fresh cells. The resulting grafts were implanted subcutaneously into nude mice to determine the phenotype stability and functionality of engineered vessels. Two important findings resulted from these studies: (i) vascular development needs to be induced prior to osteogenesis, and (ii) the addition of additional hMSCs at the osteogenic induction stage improves both tissue outcomes, as shown by increased bone volume fraction, osteoid deposition, close proximity of bone proteins to vascular networks, and anastomosis of vascular networks with the host vasculature. Interestingly, these observations compare well with what has been described for native development. We propose that our cultivation system can mimic various aspects of endothelial cell – osteogenic precursor interactions in vivo, and could find utility as a model for studies of heterotypic cellular interactions that couple blood vessel formation with osteogenesis

Measurement of neutron star parameters: a review of methods for low-mass X-ray binaries

Measurement of at least three independent parameters, for example, mass, radius and spin frequency, of a neutron star is probably the only way to understand the nature of its supranuclear core matter. Such a measurement is extremely difficult because of various systematic uncertainties. The lack of knowledge of several system parameter values gives rise to such systematics. Low-mass X-ray binaries, which contain neutron stars, provide a number of methods to constrain the stellar parameters. Joint application of these methods has a great potential to significantly reduce the systematic uncertainties, and hence to measure three independent neutron star parameters accurately. Here we review the methods based on (1) thermonuclear X-ray bursts; (2) accretion-powered millisecond-period pulsations; (3) kilohertz quasi-periodic oscillations; (4) broad relativistic iron lines; (5) quiescent emissions; and (6) binary orbital motions.Comment: 30 pages, 20 figures, 1 table, An Invited and Refereed Review, will be published in "Advances in Space Research

In Situ Grafting of Silica Nanoparticle Precursors with Covalently Attached Bioactive Agents to Form PVA-Based Materials for Sustainable Active Packaging

Sustainable antibacterial–antioxidant films were prepared using in situ graftings of silica nanoparticle (SNP) precursors with covalently attached bioactive agents benzoic acid (ba) or curcumin (cur) on polyvinyl alcohol (PVA). The modified PVA-SNP, PVA-SNP-ba and PVA-SNP-cur films were characterized using spectroscopic, physicochemical and microscopic methods. The prepared films showed excellent antibacterial and antioxidant activity, and increased hydrophobicity providing protection from undesired moisture. The PVA-SNP-ba films completely prevented the growth of the foodborne human pathogen Listeria innocua, whereas PVA-SNP-cur resulted in a 2.5 log reduction of this bacteria. The PVA-SNP-cur and PVA-SNP-ba films showed high antioxidant activity of 15.9 and 14.7 Mm/g TEAC, respectively. The described approach can serve as a generic platform for the formation of PVA-based packaging materials with tailor-made activity tuned by active substituents on silica precursors. Application of such biodegradable films bearing safe bioactive agents can be particularly valuable for advanced sustainable packaging materials in food and medicine

Adaptive and composite thin glass concepts for architectural applications

Thin glass – such as commonly applied for displays and touchscreen on electronic devices like smartphone and tablets – offers interesting characteristics for architectural applications. Due to its high strength and small thickness the glass can easily be bent in architecturally appealing curvatures, while the small thickness of the glass offers a significant weight reduction compared to traditional window glazing. This paper explores the potential of thin glass for architectural applications and reports on two thin glass concepts that are currently under investigation at TU Delft. The first concept concerns flexible and adaptive thin glass panels that can change their shape in response to external parameters. The second concept concerns thin glass composite panels in which thin glass facings are combined with (3D printed) core elements to create strong, stiff yet lightweight glass façade panels. From initial design explorations and prototyping, it can be seen that both concepts are very promising and viable for further in depth investigations