Association of mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol involves catalytic domain of the synthetase and transframe and integrase domains of Pol

Aim. Analyze the interaction between Lysyl-tRNA synthetase (LysRS) and HIV-1 GagPol to know whether a particular N-terminal sequence of mitochondrial LysRS triggers a specific recognition with GagPol. Methods. Yeast two-hybrid analysis, immunoprecipitation. Results. We have shown that LysRS associates with the Pol domain of GagPol. Conclusions. A model of the assembly of the LysRS:tRNA₃Lys:GagPol packaging complex is proposed. Keywords: tRNA₃Lys, lysyl-tRNA synthetase, HIV-1, packaging.Мета. Встановити, чи може N-кінцева послідовність, яка є специфічною для мітохондріальної форми лізил-тРНК синтетази, забезпечувати взаємодію цього ферменту з білком GagPol ВІЛ-1. Методи. Двогібридна дріжджова система, імунопреципітація. Результати. Ми показали що лізил-тРНК синтетаза взаємодіє з доменом Pol білка Gag. Висновки. Запропоновано модель утворення комплексу ЛізРС:тРНК₃Ліз:GagPol. Ключові слова: тРНК₃Ліз, лізил-тРНК синтетаза, ВІЛ-1, збирання віріона.Цель. Выяснить, может ли N-концевая последовательность, являющаяся специфичной для митохондриальной формы лизилтРНК синтетазы, обеспечивать взаимодействие этого фермента с белком GagPol ВИЧ-1. Методы. Двугибридная дрожжевая система, иммунопреципитация Результаты. Мы показали, что лизил-тРНК синтетаза взаимодействует с доменом Pol белка Gag. Выводы. Предложена модель образования комплекса ЛизРС:тРНК₃Лиз:GagPol. Ключевые слова: тРНК₃Лиз, лизил-тРНК синтетаза, ВИЧ-1, сборка вириона

Two new species of Odontostilbe historically hidden under O. microcephala (Characiformes: Cheirodontinae)

Specimens historically identified as Odontostilbe microcephala from the upper rio Paraná and Andean piedmont tributaries of the río Paraguay are reviewed and split in three species. We found that the distribution of O. microcephala is restricted to the Andean slope of the río Paraguay basin. The species is distinguished from congeners with subterminal mouth by the elongate body, usually 10-12 gill rakers on upper branch and smaller horizontal orbital diameter (24.6-32.8 % HL, mean 28.7%). Specimens from upper rio Paraná constitute two new species, diagnosed from other Cheirodontinae by the presence of mesopterygoid teeth, grouped on median portion and forming a continuous row. The new species are distinguished from each other by having premaxillary teeth with five cusps vs. nine cusps and by the number of lamellae in left and right sides of central median raphe of olfactory rosette with 20-21 vs. 11-12.Espécimes historicamente identificados com Odontostilbe microcephala do rio Paraná e tributários do río Paraguay, foram revisados e separados em três espécies. A distribuição de O. microcephala é restrita ao sopé andino da bacia do río Paraguay. A espécie é distinta das congêneres com boca subterminal pela forma alongada, geralmente 10-12 rastros branquiais no ramo superior e menor diâmetro horizontal da órbita (24,6-32,8 % CC, média 28,7%). Espécimes do alto rio Paraná constituem duas espécies novas diagnosticadas de outros Cheirodontinae pela presença de dentes no mesopterigoide, agrupados em sua porção média e formando uma fileira continua. As novas espécies distinguem-se por ter dentes premaxilares com cinco cúspides vs. nove cúspides e pelo número de lamelas nos lados esquerdo e direito da rafe central da roseta olfativa com 20-21 vs. 11-12

New species of miniature fish from Marajó Island, Pará, Brazil, with comments on its relationships (Characiformes: Characidae)

A new miniature species of the family Characidae from Marajó Island, Pará State, Brazil is described and assigned to the genus Tyttobrycon. The new species can be distinguished from its congeners primarily by having multicuspid teeth on jaws, and additionally by having the combination of 5-7 premaxillary teeth, dorsal fin only with scattered melanophores and the presence of an adipose fin