Alternative models for the calculation of the RMR and Q indexes for granite rock masses

Empirical classification systems like the RMR and Q are often used in current practice of geotechnical structures design built in rock masses. They allow obtaining an overall description of the rock mass and the calculation, through analytical solutions, of strength and deformability parameters which are determinant in design. To be applied these systems need a set of geomechanical information that may not be available or can be difficult to obtain. In this work it is intended to develop new alternative regression models for the calculation of the RMR and Q indexes using less data than the original formulations and keeping a high accuracy level. It is also intended to have an insight of which parameters are the most important for the prediction of the indexes and in the rock masses behaviour. This study was carried out applying Data Mining techniques to a database of the empirical classification systems applications in a granite rock mass. Data Mining is a relatively new area of computer science which concerns with automatically find, simplify and summarize patterns and relationships within large databases. The used Data Mining techniques were the multiple regression and artificial neural networks. The developed models are able to predict the two geomechanical indexes using less information that in the original formulations with a good predictive capacity.Fundação para a Ciência e a Tecnologia (FCT) - projecto POCI/ECM/57495/2004 "Geotechnical Risk in Tunnels for High Speed Trains

A genetic code alteration generates a proteome of high diversity in the human pathogen Candida albicans

Background - Genetic code alterations have been reported in mitochondrial, prokaryotic, and eukaryotic cytoplasmic translation systems, but their evolution and how organisms cope and survive such dramatic genetic events are not understood. Results - Here we used an unusual decoding of leucine CUG codons as serine in the main human fungal pathogen Candida albicans to elucidate the global impact of genetic code alterations on the proteome. We show that C. albicans decodes CUG codons ambiguously and tolerates partial reversion of their identity from serine back to leucine on a genome-wide scale. Conclusion - Such codon ambiguity expands the proteome of this human pathogen exponentially and is used to generate important phenotypic diversity. This study highlights novel features of C. albicans biology and unanticipated roles for codon ambiguity in the evolution of the genetic code.publishe

Mechanical analysis of asphalt mixtures produced with waste plastic modified binders

This work compares the viscoelastic properties of an asphalt binder (70/100 pen) modified with different waste plastics and the mechanical properties of the resultant asphalt mixtures. Two different plastic wastes were used, namely recycled HDPE and EVA. Three different polymer modified binders were produced with these plastic wastes: i) 5% HDPE modified binder (P5); ii) 5% EVA modified binder (E5) and; iii) a modified binder with 4% of EVA and 2% HDPE (E4P2). Asphalt mixtures were produced with these modified binders, and their mechanical properties were analysed and compared with a conventional mixture produced with a 30/50 pen bitumen. It was possible to conclude that these recycled polymers are able to improve the mechanical performance of the asphalt mixtures used in road paving.FEDER funds through the Operational Programme for Competitiveness Factors & COMPETE and National Fund s through FCT & Foundation for Science and Technology under the project PLASTIROADS (PTDC/ECM/119179/2010) and Symposium_22 Recycling and Reuse of Wastes into New Composite Materials -2062-of the strategic project UI 4047- 2011-2012 Centre of Territory, Environment and Construction

Variable speed liquid chiller drop-in modeling for predicting energy performance of R1234yf as low-GWP refrigerant

This paper presents a model for a variable-speed liquid chiller integrating a compressor model based on Buckingham π-theorem to accurately predict the system performance when R134a is replaced with R1234yf, using a wide range of data obtained from an experimental setup. Relevant variables such as temperature, pressure, mass and volumetric flow rates, compressor power consumption and rotation speed were measured at several positions along the refrigeration and secondary circuits and were used to validate the developed model. Model results show that cooling capacity and power consumption predicted values are in good agreement with experimental data, within ±5%, being slightly higher for the deviation obtained for R134a than for R1234yf. Moreover, model results indicate that R1234yf has a reduction of coefficient of performance (COP) compared with R134a (between 2 and 11.3%), and that R1234yf COP reduction is diminished at intermediate volumetric flow rate and higher inlet temperature for the evaporator secondary fluid, respectively. On the other hand, an environmental analysis based on TEWI (total equivalent warming impact) method showed that direct emissions are almost negligible for R1234yf. However, there are no environmental benefits in terms of indirect greenhouse gas emissions using R1234yf without system modifications (as for instance the addition of internal heat exchanger or R1234yf new design components), which are required to reduce the liquid chiller climate change contribution using it as low GWP alternative in comparison with the typically used R134a refrigerant

RKIP Inhibition in Cervical Cancer Is Associated with Higher Tumor Aggressive Behavior and Resistance to Cisplatin Therapy

Cervical cancer is one of the most common cancers in women worldwide, being high-risk group the HPV infected, the leading etiological factor. The raf kinase inhibitory protein (RKIP) has been associated with tumor progression and metastasis in several human neoplasms, however its role on cervical cancer is unclear. In the present study, 259 uterine cervix tissues, including cervicitis, cervical intraepithelial lesions and carcinomas, were analyzed for RKIP expression by immunohistochemistry. We found that RKIP expression was significantly decreased during malignant progression, being highly expressed in non-neoplastic tissues (54% of the samples; 73/135), and expressed at low levels in the cervix invasive carcinomas (∼15% (19/124). Following in vitro downregulation of RKIP, we observed a viability and proliferative advantage of RKIP-inhibited cells over time, which was associated with an altered cell cycle distribution and higher colony number in a colony formation assay. An in vitro wound healing assay showed that RKIP abrogation is associated with increased migratory capability. RKIP downregulation was also associated with an increased vascularization of the tumors in vivo using a CAM assay. Furthermore, RKIP inhibition induced cervical cancer cells apoptotic resistance to cisplatin treatment. In conclusion, we described that RKIP protein is significantly depleted during the malignant progression of cervical tumors. Despite the lack of association with patient clinical outcome, we demonstrate, in vitro and in vivo, that loss of RKIP expression can be one of the factors that are behind the aggressiveness, malignant progression and chemotherapy resistance of cervical cancer.</p

Lattice-matched versus lattice-mismatched models to describe epitaxial monolayer graphene on Ru (0001)

Monolayer graphene grown on Ru(0001) surfaces forms a superstructure with periodic modulations in its geometry and electronic structure. The large dimension and inhomogeneous features of this superstructure make its description and subsequent analysis a challenge for theoretical modeling based on density functional theory. In this work, we compare two different approaches to describe the same physical properties of this surface, focusing on the geometry and the electronic states confined at the surface. In the more complex approach, the actual moiré structure is taken into account by means of large unit cells, whereas in the simplest one, the graphene moiré is completely neglected by representing the system as a stretched graphene layer that adapts pseudomorphically to Ru(0001). As shown in previous work, the more complex model provides an accurate description of the existing experimental observations. More interestingly, we show that the simplified stretched models, which are computationally inexpensive, reproduce qualitatively the main features of the surface electronic structure. They also provide a simple and comprehensive picture of the observed electronic structure, thus making them particularly useful for the analysis of these and maybe other complex interfacesWe thank Barcelona Supercomputing Center–Spanish Supercomputing Network (BSC-RES) and Centro de Computación Científica – Universidad Autónoma de Madrid (CCC-UAM) for allocation of computer time. Work supported by the MICINN Projects No. FIS2010-15127, No. FIS2010-18847, No. CTQ2010-17006, No. FIS-2010-19609- C09-00, No. ACI2008-0777, No. 2010C-07-25200, and No. CSD2007-00010, the CAM program NANOBIOMAGNET S2009/MAT1726 and the Gobierno Vasco-UPV/EHU Project No. IT-366-07. S.B. acknowledges financial support from MEC under FPU Grant No. AP-2007-00157. D.S. acknowledges financial support from the FPI-UAM grant progra

Role of dispersion forces in the structure of graphene monolayers on Ru surfaces

Elaborate density functional theory (DFT) calculations that include the effect of van der Waals (vdW) interactions have been carried out for graphene epitaxially grown on Ru(0001). The calculations predict a reduction of structural corrugation in the observed moiré pattern of about 25% (∼0.4  Å) with respect to DFT calculations without vdW corrections. The simulated STM topographies are close to the experimental ones in a wide range of bias voltage around the Fermi levelWe thank Mare Nostrum BSC and CCC-UAM for computer time. Work supported by the MICINN projects FIS2010-15127, FIS2010-18847, CTQ2010-17006, FIS2010-19609-C02-00, ACI2008-0777, 2010C-07- 25200, and CSD2007-00010, the CAM program NANOBIOMAGNET S2009/MAT1726, and the Gobierno Vasco—UPV/EHU project IT-366-0

Electron localization in epitaxial graphene on Ru(0001) determined by moiré corrugation

The interpretation of scanning tunneling spectroscopy (STS) and scanning tunneling microscopy measurements of epitaxial graphene on lattice-mismatched substrates is a challenging problem, because of the spatial modulation in the electronic structure imposed by the formation of a moiré pattern. Here we describe the electronic structure of graphene adsorbed on Ru(0001) by means of density functional theory calculations that include van der Waals interactions and are performed on a large 11×11 unit cell to account for the observed moiré patterns. Our results show the existence of localized electronic states in the high and low areas of the moiré at energies close to and well above the Fermi level, respectively. Localization is due to the spatial modulation of the graphene-Ru(0001) interaction and is at the origin of the various peaks observed in STS spectraWork supported by the MICINN Projects No. FIS2010-15127, No. FIS2010-18847, No. CTQ2010-17006, No. FIS-2010-19609-C09-00, No. ACI2008-0777, No. 2010C-07-25200, and No. CSD2007-00010, the CAM program NANOBIOMAGNET S2009/MAT1726, and the Gobierno Vasco-UPV/EHU Project No. IT-366-07. S.B. acknowledges financial support from MEC under FPU Grant No. AP-2007-0015

Sublittoral soft bottom communities and diversity of Mejillones Bay in northern Chile (Humboldt Current upwelling system)

The macrozoobenthos of Mejillones Bay (23°S; Humboldt Current) was quantitatively investigated over a 7-year period from austral summer 1995/1996 to winter 2002. About 78 van Veen grab samples taken at six stations (5, 10, 20 m depth) provided the basis for the analysis of the distribution of 60 species and 28 families of benthic invertebrates, as well as of their abundance and biomass. Mean abundance (2,119 individuals m-2) was in the same order compared to a previous investigation; mean biomass (966 g formalin wet mass m-2), however, exceeded prior estimations mainly due to the dominance of the bivalve Aulacomya ater. About 43% of the taxa inhabited the complete depth range. Mean taxonomic Shannon diversity (H', Log e) was 1.54 ± 0.58 with a maximum at 20 m (1.95 ± 0.33); evenness increased with depth. The fauna was numerically dominated by carnivorous gastropods, polychaetes and crustaceans (48%). About 15% of the species were suspensivorous, 13% sedimentivorous, 11% detritivorous, 7% omnivorous and 6% herbivorous. Cluster analyses showed a significant difference between the shallow and the deeper stations. Gammarid amphipods and the polychaete family Nephtyidae characterized the 5-mzone, the molluscs Aulacomya ater, Mitrella unifasciata and gammarids the intermediate zone, while the gastropod Nassarius gayi and the polychaete family Nereidae were most prominent at the deeper stations. The communities of the three depth zones did not appear to be limited by hypoxia during non-El Niño conditions. Therefore, no typical change in community structure occurred during El Niño 1997–1998, in contrast to what was observed for deeper faunal assemblages and hypoxic bays elsewhere in the coastal Humboldt Current system