A novel HD-Zip I/C2H2-ZFP/WD-repeat complex regulates the size of spine base in cucumber

Fruit spine is an important trait in cucumber, affecting not only commercial quality, but also fruit smoothness, transportation and storage. Spine size is determined by a multi-cellular base. However, the molecular mechanism underlying the regulation of cucumber spine base remains largely unknown. Here, we report map-based cloning and characterization of a spine base size 1 (SBS1) gene, encoding a C2H2 zinc-finger transcription factor.Near-isogenic lines of cucumber were used to map, identify and quantify cucumber spine base size 1 (CsSBS1). Yeast-hybrid, bimolecular fluorescence complementation (BiFC), co-immunoprecipitation (Co-IP) and RNA-sequencing assays were used to explore the molecular mechanism of CsSBS1 in regulating spine base size development.CsSBS1 was specifically expressed in cucumber ovaries with particularly high expression in fruit spines. Overexpression of CsSBS1 resulted in large fruit spine base, while RNA-interference silencing of CsSBS1 inhibited the expansion of fruit spine base. Sequence analysis of natural cucumber accessions revealed that CsSBS1 was lost in small spine base accessions, resulting from a 4895 bp fragment deletion in CsSBS1 locus. CsSBS1 can form a trimeric complex with two positive regulators CsTTG1 and CsGL1 to regulate spine base development through ethylene signaling.A novel regulator network is proposed that the CsGL1/CsSBS1/CsTTG1 complex plays a significant role in regulating spine base formation and size, which offers a strategy for cucumber breeders to develop smooth fruit.This work was supported by the National Natural Science Foundation of China (31902020, 31972427), the Zhongyuan Youth Talent Program (ZYQR201912161), the Key Research Project of Henan institutions of higher learning (20A210015), the Program for Science & Technology Innovation Talents of Henan Province (21HASTIT038), and the Major Science and Technology Projects of Henan Province (201300111300).Peer reviewe

Asset Specificity on the Intention of Farmers to Continue Land Recuperation: Based on the Perspective of Farmer Differentiation

Land recuperation is an important institutional guarantee for green agricultural development and an important measure to promote rural revitalization. Asset specificity is a crucial factor that affects farmers’ subsequent willingness to participate in land recuperation. Based on the perspective of farmer differentiation, this study uses survey data of 605 farmers in four counties of Gansu Province and employs the entropy method and the double-hurdle model to measure asset specificity and how it affects the subsequent willingness of different types of farmers to participate in land recuperation. The results show that: (1) farmers’ willingness to participate in land recuperation increases with the degree of their part-time occupations; (2) geographical location specificity has a significant negative effect on farmers’ intention and degree of subsequent land recuperations, and the impacts on non-farmers and II part-time farmers are significantly smaller than that on pure farmers and part-time farmers; (3) physical asset specificity has the most negligible influence on farmers’ subsequent willingness to participate; (4) human capital specificity has a significant negative impact on the intention and degree of land recuperation by farmers, and the effect is more significant for pure farmers than non-farmers; (5) factors such as land recuperation compensation satisfaction, land recuperation policy trust, social connection, and off-farm employment willingness promote the subsequent land recuperation willingness and degree of land recuperation of farmers, while the cultivated land area reduces the subsequent degree of participation in land recuperation

Effects of land recuperation on farmers’ social capital: a Chinese field analysis

Social capital is an integral part of farmers’ life, which can be exogenously affected by land recuperation. Based on 1240 farmer field survey data in Gansu Province, this paper used the Logit model to analyse the influencing factors of farmers’ participation in land recuperation, and used the entropy method to measure social capital from the three dimensions of social network, social trust and social norms, and further used the propensity matching score method to measure the effect of land recuperation on farmers, and then compared the effects under different fixed ages and education groups. The following factors significantly affected farmers’ participation in land recuperation: age, years of education, migrant workers’ relationships with family and friends, relationship between migrant workers and friends and colleagues in the workplace, number of migrant workers away from home, cultivated land area, and family living standards. Land recuperation had the greatest promotion effect on farmer’ social network (163.9%), followed by social trust (28.0%) and social norm (11.3%). According to the results of group differences, land recuperation most significantly affected the social capital of farmers aged 45–55 years and household heads educated for 9–12 years compared to other age and education groups

Interfacial Reactions and Mechanical Properties Studies of C-Coated and C/B4C Duplex-Coated SiC Fiber-Reinforced Ti2AlNb Composites

Continuous SiC fiber-reinforced Ti2AlNb matrix composites have a great potential for high-temperature aviation structure applications, and their properties strongly depend on the microstructure of the interfacial reaction layer. Notably, introducing diffusion barrier coatings has still been a popular strategy for optimizing the interfacial structure and interfacial properties of SiCf/Ti. In this work, C coating and C/B4C duplex coating were successfully fabricated onto SiC fibers via chemical vapor deposition (CVD), then consolidated into the SiCf/C/Ti2AlNb and the SiCf/C/B4C/Ti2AlNb composites, respectively, via hot isostatic pressing (HIP) under the condition of 970 °C, 150 MPa, 120 min, and finally furnace cooled to room temperature. The C- and C/B4C-dominated interfacial reactions in the SiCf/C/Ti2AlNb and the SiCf/C/B4C/Ti2AlNb were explored, revealing two different reaction products sequences: The different-sized TiC and the coarse-grained (Ti,Nb)C + AlNb3 for the SiCf/C/Ti2AlNb; and the fine-grained TiB2 + TiC, the needle-shaped (Ti,Nb)B2/NbB + (Ti,Nb)C, the coarse-grained (Ti,Nb)C + AlNb2 for the SiCf/C/B4C/Ti2AlNb. Annealing experiments were further carried out to verify the different reaction kinetics caused by C coating and C/B4C duplex coating. The reaction layer (RL)-dominated interfacial strength and tensile strength estimations showed that higher interface strength and tensile strength occurred in the SiCf/C/Ti2AlNb instead of the SiCf/C/B4C/Ti2AlNb, when the same failure mode of fiber push-out took place

Integrated network pharmacology and metabolomics reveal the mechanisms of Jasminum elongatum in anti-ulcerative colitis

Abstract Jasminum elongatum (JE), an ethnic Chinese medicine, is widely used in the Lingnan region of China, because of its analgesic and antidiarrheal action, as well as its anti-inflammatory effects in gastrointestinal diseases. However, whether JE could against ulcerative colitis (UC) remains unclear. This research aims to reveal JE in treating UC and clarify the underlying mechanism. We used the 2.5% dextran sulfate sodium (DSS)-induced UC mice (C57BL/6J) to evaluate the therapeutic effects of JE. Metabolomics of serum and network pharmacology were combined to draw target-metabolite pathways. Apart from that, the targets of associated pathways were confirmed, and the mechanism of action was made clear, using immunohistochemistry. The pharmacodynamic results, including disease activity index (DAI), histological evaluation, and inflammatory cytokines in colon tissues, demonstrated that JE significantly relieved the physiological and pathological symptoms of UC. Network pharmacology analysis indicated 25 core targets, such as TNF, IL-6, PTGS2 and RELA, and four key pathways, including the NF-κB signaling pathway and arachidonic acid metabolism pathway, which were the key connections between JE and UC. Metabolomics analysis identified 45 endogenous differential metabolites and 9 metabolic pathways by enrichment, with the arachidonic acid metabolism pathway being the main metabolism pathway, consistent with the prediction of network pharmacology. IκB, p65 and COX-2 were identified as key targets and this study demonstrated for the first time that JE reverses 2.5% DSS-induced UC in mice via the IκB/p65/COX-2/arachidonic acid pathway. This study reveals the complex mechanisms underlying the therapeutic effects of JE on UC and provides a new approach to identifying the underlying mechanisms of the pharmacological action of Chinese natural medicines such as JE

Submicroscopic chromosomal imbalances contribute to early abortion

Abstract Background Chromosomal abnormalities are one of the genetic mechanisms associated with abortion. However, the roles of submicroscopic chromosomal imbalances in early abortion are still unclear. This study aims to find out whether submicroscopic chromosomal imbalances contribute to early abortion. Methods A total of 78 chorionic villus specimens from early spontaneous abortion patients with no obvious abnormality are collected after miccroassay analysis (the case group). At the same time, 60 chorionic villus specimens from induced abortion patients with no obvious abnormality are selected as the control group. The submicroscopic structures of chromosomes from two groups are analyzed using an array-based comparative genomic hybridization (aCGH). Results In the case group, 15 specimens show submicroscopic chromosomal abnormalities including 14 micro-deletion/micro-duplication in chromosomes 2, 4, 5, 6, 7, 8, 9, 12, 15, 16, 18, and 22, and 1 uniparental disomy (UPD) in chromosome 19. Moreover, no pathogenic copy number variations are found in the control group. The results between these two groups exhibit significantly statistical difference. Conclusion Submicroscopic chromosomal imbalances may be one of the main reasons for early abortion