Comprehensive analysis reveals CCDC60 as a potential biomarker correlated with prognosis and immune infiltration of head and neck squamous cell carcinoma

BackgroundCoiled-coil domain containing 60 (CCDC60) is a member of the CCDC family, which participates in the progression of many types of cancer. However, the prognostic value of CCDC60 in head and neck squamous cell carcinoma (HNSC) and its function in tumor immunity remain unclear.MethodsCCDC60 expression and its prognostic potential in HNSC were evaluated by bioinformatics approaches, which was validated in human HNSC samples. Genetic alteration analysis of CCDC60 and the underlying biological function of CCDC60 related co-expressed genes in HNSC were analyzed. The impact of CCDC60 on the regulation of immune infiltration in HNSC was comprehensively investigated. In vitro, a series of functional assays on CCDC60 were performed in HNSC cells.ResultsOur study has indicated that compared with the adjacent normal tissues, CCDC60 expression was considerably downregulated in HNSC tissues. High CCDC60 expression was connected with favorable outcome of HNSC patients, and its prognostic significance was examined by distinct clinical characteristics. We identified the CCDC60-related co-expression genes, which were mainly enriched in the NOD-like receptor signaling pathway associated with the inhibition of tumor growth, leading to a better prognosis of HNSC patients. In vitro, CCDC60 overexpression significantly inhibited the growth, migration and invasiveness but regulated cell cycle progression, and promoted cell adhesion of Fadu and Cal27 cells. Additionally, high CCDC60 expression had strong connections with the infiltrating levels of immune cells, immune marker sets, immunomodulators and chemokines in HNSC, suggesting that targeting CCDC60 could be a promising strategy to enhance the efficacy of immunotherapy for HNSC patients.ConclusionTumor suppressor CCDC60 may be identified as a prognostic and immune-related indicator in HNSC, which had the potential functions in regulating the immune infiltration of HNSC and improving the response to immunotherapy for HNSC patients

Mask CycleGAN: Unpaired Multi-modal Domain Translation with Interpretable Latent Variable

We propose Mask CycleGAN, a novel architecture for unpaired image domain translation built based on CycleGAN, with an aim to address two issues: 1) unimodality in image translation and 2) lack of interpretability of latent variables. Our innovation in the technical approach is comprised of three key components: masking scheme, generator and objective. Experimental results demonstrate that this architecture is capable of bringing variations to generated images in a controllable manner and is reasonably robust to different masks

Research on fault recovery method of AC/DC hybrid distribution network with electric vehicle charging station

It is very effective to realize fault recovery through the advantages of DC network after the fault occurs in the AC/DC hybrid distribution network. The power transfer can be realized in space and time, through the coordination of voltage source converter (VSC), network reconfiguration and electric vehicle charging station (EVCS). In order to realize the fault recovery of AC/DC hybrid distribution network, the faults are divided into five types according to the fault locations and the connection characteristics between outage areas and power supply. The selection principles of the corresponding VSC control mode set are summarized, and all feasible VSC control modes are obtained. A mixed integer programming model for fault recovery of AC/DC hybrid distribution network with EVCS is established, which takes the minimum weighted outage power of loads as the optimization objective. The power supply recovery is realized from two aspects of space and time, and the effect of fault recovery is greatly improved. The results show that the total weighted outage loads of the proposed strategy are 14.98% lower than only using VSCs and reconfiguration

Disc swirl spinning — A novel approach

179-183A new spinning method ‘disc swirl spinning’, which consists of a combing roller drafting device, funnel fibre conveyer, disc fibre collector and swirl twister, has been developed. The spinning process, the design of the mechanism and its applications have been described and a preliminary comparative performance of the yarn quality analyzed. Scanning electron microphotographs of the disc swirl core-spun yarn have been compared with those of the air-jet spun and rotor-spun yarns. It is observed that the appearance of disc swirl core-spun yarn is similar to that of rotor-spun yarn, while the inner structure of the disc swirl core-spun yarn is found similar to that of the air-jet spun yarn. The breaking strength of disc swirl core-spun yarn is little lower than that of the ring core-spun yarn, while its breaking elongation CV﹪ is higher. The other quality parameters of the two kinds of yarn are found to be similar. The quality of the disc swirl core-spun yarn can basically meet the need of the processes

CYP4F12 is a potential biomarker and inhibits cell migration of head and neck squamous cell carcinoma via EMT pathway

Abstract Head and neck squamous cell carcinoma (HNSC) is the most common malignant tumor of head and neck. Due to the insidious nature of HNSC and the lack of effective early diagnostic indicators, the development of novel biomarkers to improve patient prognosis is particularly urgent. In this study, we explored and validated the correlation between cytochrome P450 family 4 subfamily F member 12 (CYP4F12) expression levels and HNSC progression using data from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) datasets and collected patient samples. We analyzed the association of CYP4F12 expression with clinicopathological features, immune correlation and prognosis. Finally, we analyzed the correlation between CYP4F12 and pathways, and verified by experiments. The results showed that CYP4F12 was low expressed in tumor tissues, participated in a variety of phenotypic changes of HNSC and affected immune cell infiltration. Pathway analysis indicated that CYP4F12 may play a key role in tumor cell migration and apoptosis. Experimental results showed that over-expression of CYP4F12 inhibited cell migration and enhanced the adhesion between cells and matrix by inhibiting epithelial-mesenchymal transition (EMT) pathway in HNSC cells. In conclusion, our study provided insights into the role of CYP4F12 in HNSC and revealed that CYP4F12 may be a potential therapeutic target for HNSC

Matrixing Network and Distributed Computing in the Simulation of Fishing Nets

AbstractMatrixing network was developed in the simulation of fishing nets. We illustrated that it was a high efficient data structure in the engineering computation of such pelagic fishing gears as purse seine and trawl. Based on this matrixing network, distributed computing was employed in solving the huge mathematical models. The results indicated that distributed computing could logically and efficiently arrange the calculations, and the matrixing data gave great advantages in computations in computer. The results showed that knot model was easier and quicker to reach equilibrium state. More mass points with delicate distributions might be responsible for this phenomenon. The less stability for knotless purse seine in actual fishing at sea was also consistent with the simulations