Discriminatory Filtering: CIPA\u27s Effect on Our Nation\u27s Youth and Why the Supreme Court Erred in Upholding the Constitutionality of The Children\u27s Internet Protection Act

Congress introduced the Children\u27s Internet Protection Act ( CIPA ) in order to filter obscene and indecent material in response to a perceived threat to members of the public, specifically minors, who are exposed to pornographic material on the Internet. The provisions of CIPA have provoked tension between two competing interests: protecting minors from cyberpornography, and safeguarding First Amendment rights. This Note argues that the Supreme Court erred by upholding the constitutionality of CIPA. As a result of the Supreme Court\u27s decision, the nation\u27s youth will have restricted access to constitutionally protected information. The Court improperly relied on a provision of the Act permitting adults to request that library filters be disabled upon request. Part II of this Note provides background on CIPA and the litigation surrounding it. The decisions of both the district court and the Supreme Court are analyzed in detail. Part III explains what an Internet filter is and how filters work in the context of CIPA. Part IV identifies the burdens that libraries face as a consequence of CIPA. Part V recognizes several less restrictive alternatives to the implementation of CIPA. Part VI expounds upon the substantial effect that CIPA will have on today\u27s youth. The Note concludes by arguing that CIPA simply does not accomplish what it was designed to do

Discriminatory Filtering: CIPA\u27s Effect on Our Nation\u27s Youth and Why the Supreme Court Erred in Upholding the Constitutionality of The Children\u27s Internet Protection Act

Congress introduced the Children\u27s Internet Protection Act ( CIPA ) in order to filter obscene and indecent material in response to a perceived threat to members of the public, specifically minors, who are exposed to pornographic material on the Internet. The provisions of CIPA have provoked tension between two competing interests: protecting minors from cyberpornography, and safeguarding First Amendment rights. This Note argues that the Supreme Court erred by upholding the constitutionality of CIPA. As a result of the Supreme Court\u27s decision, the nation\u27s youth will have restricted access to constitutionally protected information. The Court improperly relied on a provision of the Act permitting adults to request that library filters be disabled upon request. Part II of this Note provides background on CIPA and the litigation surrounding it. The decisions of both the district court and the Supreme Court are analyzed in detail. Part III explains what an Internet filter is and how filters work in the context of CIPA. Part IV identifies the burdens that libraries face as a consequence of CIPA. Part V recognizes several less restrictive alternatives to the implementation of CIPA. Part VI expounds upon the substantial effect that CIPA will have on today\u27s youth. The Note concludes by arguing that CIPA simply does not accomplish what it was designed to do

Stabilization of microbial residues by co-precipitation with Fe and Al oxyhydroxides

It is now widely accepted that microbial residues are a significant source for soil organic matter (SOM) formation. This material must be stabilised in soil in order to persist. A potential mechanism for stabilisation of organic materials in soil is co-precipitation with metal oxyhydroxides (Fe and Al), which, however, may be affected by redox transitions. We thus evaluated the mineralisation of 14C-labelled bacterial residues (Escherichia coli cells and cell envelope fragments) and their co-precipitates with Fe or Al oxyhydroxide under different redox conditions in a laboratory incubation experiment. The co-precipitates or untreated microbial residues (control) were mixed with soil and incubated in sealed vessels under either fully aerobic or under oxygen-limited conditions for up to 345 days. To achieve oxygen limitation, incubation was conducted under an N2 atmosphere for the first 100 days. The redox potential was further decreased by waterlogging the samples (from day 100) and by substrate and nutrient additions (from day 290), to increase electron acceptor consumption by the soil microbes. Mineralisation of the microbial residues was quantified by liquid scintillation counting. The data were fitted to different types of models, depending on the experimental phase. Co-precipitation with Fe and Al oxyhydroxides decreased mineralisation of both intact cells and cell envelope fragments significantly, indicating strong protection of biomass and its fragments. Mineralisation of intact cells was slightly faster than that of cell envelope fragments, indicating higher recalcitrance of the latter material, which therefore may be enriched in SOM. Strongly reducing conditions resulted reductive dissolution of Fe oxyhydroxide and thus in a loss of the stabilising effect of the co-precipitation. We conclude that co-precipitation with and incrustation of organic material by Fe and Al oxyhydroxides provide significant stabilisation of microbial residues. However, environmental conditions, e.g. the redox potential, modify the extent of this stabilisation. Fitting the mineralisation data to the models indicated that initially mainly pool sizes were affected by the factors studied, whereas later in the experiment the rate constants were more sensitive. The results improved significantly our understanding how organic materials, in particular microbial residues, are stabilised in soil

Put Down that Phone and Talk to Me: Understanding the Roles of Mobile Phone Norm Adherence and Similarity in Relationships

This is the author's accepted manuscript. The published version will be available in 2014 from http://www.sagepub.com/journals/Journal202140.This study uses co-orientation theory to examine the impact of mobile phone use on relational quality across three co-present contexts. It investigates the relationship between perceived similarity, actual similarity, and understanding of mobile phone usage on relationship outcomes, and uses a new measure of mobile relational interference to assess how commitment, satisfaction, and liking are affected by perceptions of relational partners' mobile phone use. Contrary to popular belief, the results from this study of 69 dyads reveals that, at least within a sample of young Americans, failing to adhere to injunctive (i.e., societal) norms regarding mobile phone usage does not impact relational quality. Rather, results indicate that perceived adherence to participants' own internal standards —by both the participant, and the participant's relational partner— and perceived similarity between partners were more influential. Keywords: commitment; co-orientation theory; etiquette; liking; mobile phone; satisfactio

Lignin dynamics in two13C-labelled arable soils during 18 years

Lignin has long been considered a relatively stable component of soil organic matter. However, recent studies suggest that lignin may turn over within years to decades in arable soil. Here we analyzed lignin concentrations in an 18 year field experiment under continuous silage maize where two soils were sampled at six points in time. Our objectives were to examine the long-term dynamics of (i) lignin derived from a previous C3-vegetation and (ii) lignin derived from maize, as influenced by two levels of maize biomass input. Total lignin concentrations in soil were quantified by gas chromatography of lignin cupric oxide oxidation products. Compound-specific 13C isotope analysis allowed discrimination between C3-derived lignin and maize-derived lignin. Degradation dynamics of C3-derived lignin were independent of biomass input level, suggesting that priming did not affect soil lignin concentrations over almost two decades. After 18 years approximately two thirds of the initial C3-derived lignin remained in the soils, whereas, on average, 10 % of the recent maize-derived lignin input was retained. We suggest that lignin is effectively stabilized in these arable soils, although the mechanisms involved remain unclear

GABA-enhanced collective behavior in neuronal axons underlies persistent gamma-frequency oscillations

Gamma (30–80 Hz) oscillations occur in mammalian electroencephalogram in a manner that indicates cognitive relevance. In vitro models of gamma oscillations demonstrate two forms of oscillation: one occurring transiently and driven by discrete afferent input and the second occurring persistently in response to activation of excitatory metabotropic receptors. The mechanism underlying persistent gamma oscillations has been suggested to involve gap-junctional communication between axons of principal neurons, but the precise relationship between this neuronal activity and the gamma oscillation has remained elusive. Here we demonstrate that gamma oscillations coexist with high-frequency oscillations (>90 Hz). High-frequency oscillations can be generated in the axonal plexus even when it is physically isolated from pyramidal cell bodies. They were enhanced in networks by nonsomatic -aminobutyric acid type A (GABAA) receptor activation, were modulated by perisomatic GABAA receptor-mediated synaptic input to principal cells, and provided the phasic input to interneurons required to generate persistent gamma-frequency oscillations. The data suggest that high-frequency oscillations occurred as a consequence of random activity within the axonal plexus. Interneurons provide a mechanism by which this random activity is both amplified and organized into a coherent network rhythm

Cell death sensitization of leukemia cells by opioid receptor activation

Cyclic AMP (cAMP) regulates a number of cellular processes and modulates cell death induction. cAMP levels are altered upon stimulation of specific G-protein-coupled receptors inhibiting or activating adenylyl cyclases. Opioid receptor stimulation can activate inhibitory Gi-proteins which in turn block adenylyl cyclase activity reducing cAMP. Opioids such as D,L-methadone induce cell death in leukemia cells. However, the mechanism how opioids trigger apoptosis and activate caspases in leukemia cells is not understood. In this study, we demonstrate that downregulation of cAMP induced by opioid receptor activation using the opioid D,L-methadone kills and sensitizes leukemia cells for doxorubicin treatment. Enhancing cAMP levels by blocking opioid-receptor signaling strongly reduced D,L-methadone-induced apoptosis, caspase activation and doxorubicin-sensitivity. Induction of cell death in leukemia cells by activation of opioid receptors using the opioid D,L-methadone depends on critical levels of opioid receptor expression on the cell surface. Doxorubicin increased opioid receptor expression in leukemia cells. In addition, the opioid D,L-methadone increased doxorubicin uptake and decreased doxorubicin efflux in leukemia cells, suggesting that the opioid D,L-methadone as well as doxorubicin mutually increase their cytotoxic potential. Furthermore, we found that opioid receptor activation using D,L-methadone alone or in addition to doxorubicin inhibits tumor growth significantly in vivo. These results demonstrate that opioid receptor activation via triggering the downregulation of cAMP induces apoptosis, activates caspases and sensitizes leukemia cells for doxorubicin treatment. Hence, opioid receptor activation seems to be a promising strategy to improve anticancer therapies

Opioid receptor activation triggering downregulation of cAMP improves effectiveness of anti-cancer drugs in treatment of glioblastoma

Glioblastoma are the most frequent and malignant human brain tumors, having a very poor prognosis. The enhanced radio- and chemoresistance of glioblastoma and the glioblastoma stem cells might be the main reason why conventional therapies fail. The second messenger cyclic AMP (cAMP) controls cell proliferation, differentiation, and apoptosis. Downregulation of cAMP sensitizes tumor cells for anti-cancer treatment. Opioid receptor agonists triggering opioid receptors can activate inhibitory Gi proteins, which, in turn, block adenylyl cyclase activity reducing cAMP. In this study, we show that downregulation of cAMP by opioid receptor activation improves the effectiveness of anti-cancer drugs in treatment of glioblastoma. The µ-opioid receptor agonist D,L-methadone sensitizes glioblastoma as well as the untreatable glioblastoma stem cells for doxorubicin-induced apoptosis and activation of apoptosis pathways by reversing deficient caspase activation and deficient downregulation of XIAP and Bcl-xL, playing critical roles in glioblastomas' resistance. Blocking opioid receptors using the opioid receptor antagonist naloxone or increasing intracellular cAMP by 3-isobutyl-1-methylxanthine (IBMX) strongly reduced opioid receptor agonist-induced sensitization for doxorubicin. In addition, the opioid receptor agonist D,L-methadone increased doxorubicin uptake and decreased doxorubicin efflux, whereas doxorubicin increased opioid receptor expression in glioblastomas. Furthermore, opioid receptor activation using D,L-methadone inhibited tumor growth significantly in vivo. Our findings suggest that opioid receptor activation triggering downregulation of cAMP is a promising strategy to inhibit tumor growth and to improve the effectiveness of anti-cancer drugs in treatment of glioblastoma and in killing glioblastoma stem cells