526 research outputs found

    Combined electrochemistry and Raman spectroscopy of heme proteins

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    Gooijer, C. [Promotor]Zwan, G. van der [Copromotor

    An Ultra-Stable Referenced Interrogation System in the Deep Ultraviolet for a Mercury Optical Lattice Clock

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    We have developed an ultra-stable source in the deep ultraviolet, suitable to fulfill the interrogation requirements of a future fully-operational lattice clock based on neutral mercury. At the core of the system is a Fabry-P\'erot cavity which is highly impervious to temperature and vibrational perturbations. The mirror substrate is made of fused silica in order to exploit the comparatively low thermal noise limits associated with this material. By stabilizing the frequency of a 1062.6 nm Yb-doped fiber laser to the cavity, and including an additional link to LNE-SYRTE's fountain primary frequency standards via an optical frequency comb, we produce a signal which is both stable at the 1E-15 level in fractional terms and referenced to primary frequency standards. The signal is subsequently amplified and frequency-doubled twice to produce several milliwatts of interrogation signal at 265.6 nm in the deep ultraviolet.Comment: 7 pages, 6 figure

    Ultralow phase noise microwave generation with an Er:fiber-based optical frequency divider

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    We present an optical frequency divider based on a 200 MHz repetition rate Er:fiber mode-locked laser that, when locked to a stable optical frequency reference, generates microwave signals with absolute phase noise that is equal to or better than cryogenic microwave oscillators. At 1 Hz offset from a 10 GHz carrier, the phase noise is below -100 dBc/Hz, limited by the optical reference. For offset frequencies > 10 kHz, the phase noise is shot noise limited at -145 dBc/Hz. An analysis of the contribution of the residual noise from the Er:fiber optical frequency divider is also presented.Comment: 4 pages, 3 figure

    Ultrastable lasers based on vibration insensitive cavities

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    We present two ultra-stable lasers based on two vibration insensitive cavity designs, one with vertical optical axis geometry, the other horizontal. Ultra-stable cavities are constructed with fused silica mirror substrates, shown to decrease the thermal noise limit, in order to improve the frequency stability over previous designs. Vibration sensitivity components measured are equal to or better than 1.5e-11 per m.s^-2 for each spatial direction, which shows significant improvement over previous studies. We have tested the very low dependence on the position of the cavity support points, in order to establish that our designs eliminate the need for fine tuning to achieve extremely low vibration sensitivity. Relative frequency measurements show that at least one of the stabilized lasers has a stability better than 5.6e-16 at 1 second, which is the best result obtained for this length of cavity.Comment: 8 pages 12 figure

    Amplitude to phase conversion of InGaAs pin photo-diodes for femtosecond lasers microwave signal generation

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    When a photo-diode is illuminated by a pulse train from a femtosecond laser, it generates microwaves components at the harmonics of the repetition rate within its bandwidth. The phase of these components (relative to the optical pulse train) is known to be dependent on the optical energy per pulse. We present an experimental study of this dependence in InGaAs pin photo-diodes illuminated with ultra-short pulses generated by an Erbium-doped fiber based femtosecond laser. The energy to phase dependence is measured over a large range of impinging pulse energies near and above saturation for two typical detectors, commonly used in optical frequency metrology with femtosecond laser based optical frequency combs. When scanning the optical pulse energy, the coefficient which relates phase variations to energy variations is found to alternate between positive and negative values, with many (for high harmonics of the repetition rate) vanishing points. By operating the system near one of these vanishing points, the typical amplitude noise level of commercial-core fiber-based femtosecond lasers is sufficiently low to generate state-of-the-art ultra-low phase noise microwave signals, virtually immune to amplitude to phase conversion related noise.Comment: 7 pages, 6 figures, submitted to Applied Physics

    Ultra-low noise microwave generation with fiber-based optical frequency comb and application to atomic fountain clock

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    We demonstrate the use of a fiber-based femtosecond laser locked onto an ultra-stable optical cavity to generate a low-noise microwave reference signal. Comparison with both a liquid Helium cryogenic sapphire oscillator (CSO) and a Ti:Sapphire-based optical frequency comb system exhibit a stability about 3×10153\times10^{-15} between 1 s and 10 s. The microwave signal from the fiber system is used to perform Ramsey spectroscopy in a state-of-the-art Cesium fountain clock. The resulting clock system is compared to the CSO and exhibits a stability of 3.5×1014τ1/23.5\times10^{-14}\tau^{-1/2}. Our continuously operated fiber-based system therefore demonstrates its potential to replace the CSO for atomic clocks with high stability in both the optical and microwave domain, most particularly for operational primary frequency standards.Comment: 3 pages, 3 figure

    Ethylene Forming Activity from ACC in Citrus Leaf Discs: Influence of Light and Darkness

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    The influence of light and darkness incubation on ethylene forming activity from 1-aminocyclopropane-1-carboxylic acid (ACC) in citrus (Citrus sinensis L. Osbeck cv. 'Salustiana) mature leaf discs was studied. Leaf discs incubated 48 hours inlight produced 20 times greater ethylene than in darkness. Twenty-four hours light and darkness alternative incubations were carried out. In any case, transference of discs from the light to the dark resulted in inhibition of ethylene forming activity. Effects of DCMU (3-(3,4- dichlorophenyl)-1,1-dimethylurea, inhibitor of photosynthetic electron flow) and KCN (inhibitor of cytochrome oxidase) were studied,, DCMU at 0.1 mM concentration inhibited ethylene forming activity after 48 h incubation in light at 95%.However, ethylene forming activity was not affected by DCMU in the dark. On the other hand, 1mMKCN stimulated considerably ethylene forming activity both in the light and dark. Incubation in a CO, enriched atmosphere did not affect ethylene forming activity in light. Therefore, respiratory CO, release could not be the responsible of ethylene forming activity inhibition in the dark. Increase on ethylene production in light from ACC in mature leaf discs is related with the ethylene forming enzyme (EFE) because of CO, + ion (inhibitor of EFE activity) reduced highly ethylene production from ACC both in the light and dark. Likewise mannitol (stimulator of EFE activity and ACC synthesis) enhanced ethylene production from ACC both in the light and in the dark. Cycloheximide (inhibitor of protein synthesis) also inhibited ethylene production from ACC. Therefore, enzyme synthesis could be required for the ethylene forming activity from ACC
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