IS YIELD RESPONSE SITE-SPECIFIC? REVISITING NITROGEN RECOMMENDATIONS ON CORN

Replaced with revised version of paper 08/19/02.Crop Production/Industries,

SITE-SPECIFIC VERSUS WHOLE-FIELD FERTILITY AND LIME MANAGEMENT IN MICHIGAN SOYBEANS AND CORN

Prior research into variable-rate application (VRA) of fertilizer nutrients has found profitability to be lacking in single nutrient applications to U.S. cereal crops. This study examines the yield and cost effects of VRA phosphorus, potassium and lime application on Michigan corn and soybean farm fields in 1998-2001. After four years, we found no yield gain from site-specific management, but statistically significant added costs, resulting in no gain in profitability. Contrary to results elsewhere, there was no evidence of enhanced spatial yield stability due to site-specific fertility management. Likewise, there was no evidence of decreased variability of phosphorus, potassium or lime after VRA treatment. Site-specific response functions and yield goals might also enhance the likelihood of profitable VRA in the future.Crop Production/Industries,

Using the acoustic peak to measure cosmological parameters

Recent measurements of the cosmic microwave background radiation by the Boomerang experiment indicate that the universe is spatially flat. Here some simple back-of-the-envelope calculations are used to explain their result. The main result is a simple formula for the angular scale of the acoustic peak in terms of the standard cosmological parameters: l=193*[1+3(1-Omega_0)/5+(1-h)/5+Omega_Lambda/35].Comment: 4 pages, 1 figure, Explanations have been clarifie

Reply to comment on the paper by Davies et al. “Resolving MISS conceptions and misconceptions: A geological approach to sedimentary surface textures generated by microbial and abiotic processes”

We thank Noffke (2017) for her comment and for providing an opportunity to clarify our classification of “sedimentary surface textures”. We accord great credit to Dr. Noffke and other dedicated researchers whose detailed work has brought microbially induced sedimentary structures (MISS) to the widespread attention of geoscientists. However, we stand by our assertion that attributing structures observed in practical field and laboratory studies to processes of formation is much more problematic than Noffke (2017) indicates. Indeed, points in the Comment confirm the need for a classification system that categorises the degree of certainty attributed to a given interpretation. We stress that our paper was not designed as a critique of previous studies of MISS but rather was designed to encourage a reasonable assessment of uncertainty in assigning sedimentary surface textures to physical processes or to MISS

Resolving MISS conceptions and misconceptions: A geological approach to sedimentary surface textures generated by microbial and abiotic processes

The rock record contains a rich variety of sedimentary surface textures on siliciclastic sandstone, siltstone and mudstone bedding planes. In recent years, an increasing number of these textures have been attributed to surficial microbial mats at the time of deposition, resulting in their classification as microbially induced sedimentary structures, or MISS. Research into MISS has developed at a rapid rate, resulting in a number of misconceptions in the literature. Here, we attempt to rectify these MISS misunderstandings. The first part of this paper surveys the stratigraphic and environmental range of reported MISS, revealing that contrary to popular belief there are more reported MISS-bearing rock units of Phanerozoic than Precambrian age. Furthermore, MISS exhibit a pan-environmental and almost continuous record since the Archean. Claims for the stratigraphic restriction of MISS to intervals prior to the evolution of grazing organisms or after mass extinction events, as well as claims for the environmental restriction of MISS, appear to result from sampling bias. In the second part of the paper we suggest that raised awareness of MISS has come at the cost of a decreasing appreciation of abiotic processes that may create morphologically similar features. By introducing the umbrella term ‘sedimentary surface textures’, of which MISS are one subset, we suggest a practical methodology for classifying such structures in the geological record. We illustrate how elucidating the formative mechanisms of ancient sedimentary surface textures usually requires consideration of a suite of sedimentological evidence from surrounding strata. Resultant interpretations, microbial or non-microbial, should be couched within a reasonable degree of uncertainty. This approach recognizes that morphological similarity alone does not constitute scientific proof of a common origin, and reinstates a passive descriptive terminology for sedimentary surface textures that cannot be achieved with the current MISS lexicon. It is hoped that this new terminology will reduce the number of overly sensational and misleading claims of MISS occurrence, and permit the means to practically separate initial observation from interpretation. Furthermore, this methodology offers a scientific approach that appreciates the low likelihood of conclusively identifying microbial structures from visual appearance alone, informing the search for true MISS in Earth's geological record and potentially on other planetary bodies such as Mars

Sidechain control of porosity closure in multiple peptide-based porous materials by cooperative folding

Porous materials find application in separation, storage and catalysis. We report a crystalline porous solid formed by coordination of metal centres with a glycylserine dipeptide. We prove experimentally that the structure evolves from a solvated porous into a non-porous state as result of ordered displacive and conformational changes of the peptide that suppress the void space in response to environmental pressure. This cooperative closure, which recalls the folding of proteins, retains order in three-dimensions and is driven by the hydroxyl groups acting as H-bond donors in the peptide sequence through the serine residue. This ordered closure is also displayed by multipeptide solid solutions in which the combination of different sequences of amino acids controls their guest response in a non-linear way. This functional control can be compared to the effect of single point mutations in proteins, where the exchange of single amino acids can radically alter structure and functio

Simultaneous determination of regional left ventricular wall stresses in intact canine hearts

Von Hans Walte

Proteomic approach to identify candidate effector molecules during the in vitro immune exclusion of infective Teladorsagia circumcincta in the abomasum of sheep

International audienceIn the present study we have employed an in vitro organ challenge model to study the post-challenge responses in parasite naïve and immune gastric tissue of sheep, in an attempt to identify the host derived factors involved in immune exclusion of Teladorsagia circumcincta larvae. Proteins present in the epithelial cells and mucus from ovine abomasa following parasite challenge in previously naïve and immune animals were analysed through Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI-Tof)-MS and shotgun proteomics. MALDI-ToF analysis of epithelial cell lysates revealed that a number of proteins identified were differentially expressed in naïve and immune cells. These included intelectin and lysozymes, which were present at higher levels in epithelial cell lysates derived from immune samples. A large number of proteins were identified in the mucosal wash from immune tissue which were not present in the mucosal wash of the naïve tissue. Some of these proteins were present in washes of immune tissue prior to the parasite challenge including immunoglobulin A, galectin 14 and 15 and sheep mast cell protease 1. However, other proteins, such as calcium activated chloride channel and intelectin were only detected in the washings from the challenged tissue. The latter may be related to an enhanced mucus release, which may result in entrapment of infective larvae and thus reduced establishment in tissue that has been previously challenged with the parasite. In conclusion, several proteins have been identified which may be involved, either directly or indirectly, in the exclusion and immune elimination of incoming infective larvae. In the present study, the usefulness of the in vitro model has been confirmed, and the global proteomic approach has identified proteins that had not previously been associated with parasite exclusion from abomasal mucosa, such as the calcium activated chloride channel