The Write Stuff: The Plausible Capability of Jesus’ Followers to Author the Gospels

It is common for critics of the New Testament to cite William Harris’ 10% literacy rate for first-century Greco-Romans as evidence for the implausibility of Jesus’ followers to write, publish, and circulate the New Testament. This “evidence” is often used to dismiss the entire New Testament as a second-century fabrication that cannot accurately represent the true teachings of Jesus. Is this an accurate portrayal of Galilee during the time of Jesus? The purpose of this article is to demonstrate that Jesus’ followers possessed the “‘Write’ Stuff”—the ability to read, write, and memorize, as well as, access to the technology needed to produce and publish the New Testament

Rapid Replacement of Bridge Deck Expansion Joints Study – Phase I

Bridge deck expansion joints are used to allow for movement of the bridge deck due to thermal expansion, dynamics loading, and other factors. More recently, expansion joints have also been utilized to prevent the passage of winter de-icing chemicals and other corrosives applied to bridge decks from penetrating and damaging substructure components of the bridge. Expansion joints are often one of the first components of a bridge deck to fail and repairing or replacing expansion joints are essential to extending the life of any bridge. In the Phase I study, the research team focused on the current means and methods of repairing and replacing bridge deck expansion joints. Research team members visited with Iowa Department of Transportation (DOT) Bridge Crew Leaders to document methods of maintaining and repairing bridge deck expansion joints. Active joint replacement projects around Iowa were observed to document the means of replacing expansion joints that were beyond repair, as well as, to identify bottlenecks in the construction process that could be modified to decrease the length of expansion joint replacement projects. After maintenance and replacement strategies had been identified, a workshop was held at the Iowa State Institute for Transportation to develop ideas to better maintain and replace expansion joints. Maintenance strategies were included in the discussion as a way to extend the useful life of a joint, thus decreasing the number of joints replaced in a year and reducing the traffic disruptions

\u3ci\u3ePostmodernism, “Reality” and Public Administration: A Discourse\u3c/i\u3e

Chapter, Deconstructing Administrative Behavior: The “Real” as Representation, authored by Gary Marshall, UNO faculty member.https://digitalcommons.unomaha.edu/facultybooks/1237/thumbnail.jp

Experimental Line Parameters of the b^(1)Σ^(+)_g ← X^(3)Σ^(-)_g Band of Oxygen Isotopologues at 760 nm Using Frequency-Stabilized Cavity Ring-Down Spectroscopy

Positions, intensities, self-broadened widths, and collisional narrowing coefficients of the oxygen isotopologues ^(16)O^(18)O, ^(16)O^(17)O, ^(17)O^(18)O, and ^(18)O^(18)O have been measured for the b^(1)Σg + ← X^(3)Σg − (0,0) band using frequency-stabilized cavity ring-down spectroscopy. Line positions of 156 P-branch transitions were referenced against the hyperfine components of the ^(39)K D_1 (4s ^(2)S_(1/2) → 4p ^(2)P_(1/2)) and D_2 (4s ^(2)S_(1/2) → 4p ^(2)P_(3/2)) transitions, yielding precisions of ~0.00005 cm^(−1) and absolute accuracies of 0.00030 cm^(−1) or better. New excited b^(1)Σg + state molecular constants are reported for all four isotopologues. The measured line intensities of the ^(16)O^(18)O isotopologue are within 2% of the values currently assumed in molecular databases. However, the line intensities of the ^(16)O^(17)O isotopologue show a systematic, J-dependent offset between our results and the databases. Self-broadening half-widths for the various isotopologues are internally consistent to within 2%. This is the first comprehensive study of the line intensities and shapes for the ^(17)O^(18)O or ^(18)O_2 isotopologues of the b^(1)Σg + ← X^(3)Σg − (0,0) band of O_2. The ^(16)O_2, ^(16)O^(18)O, and ^(16)O^(17)O line parameters for the oxygen A-band have been extensively revised in the HITRAN 2008 database using results from the present study

Identifying components of the hair-cell interactome involved in cochlear amplification

<p>Abstract</p> <p>Background</p> <p>Although outer hair cells (OHCs) play a key role in cochlear amplification, it is not fully understood how they amplify sound signals by more than 100 fold. Two competing or possibly complementary mechanisms, stereocilia-based and somatic electromotility-based amplification, have been considered. Lacking knowledge about the exceptionally rich protein networks in the OHC plasma membrane, as well as related protein-protein interactions, limits our understanding of cochlear function. Therefore, we focused on finding protein partners for two important membrane proteins: Cadherin 23 (cdh23) and prestin. Cdh23 is one of the tip-link proteins involved in transducer function, a key component of mechanoelectrical transduction and stereocilia-based amplification. Prestin is a basolateral membrane protein responsible for OHC somatic electromotility.</p> <p>Results</p> <p>Using the membrane-based yeast two-hybrid system to screen a newly built cDNA library made predominantly from OHCs, we identified two completely different groups of potential protein partners using prestin and cdh23 as bait. These include both membrane bound and cytoplasmic proteins with 12 being <it>de novo </it>gene products with unknown function(s). In addition, some of these genes are closely associated with deafness loci, implying a potentially important role in hearing. The most abundant prey for prestin (38%) is composed of a group of proteins involved in electron transport, which may play a role in OHC survival. The most abundant group of cdh23 prey (55%) contains calcium-binding domains. Since calcium performs an important role in hair cell mechanoelectrical transduction and amplification, understanding the interactions between cdh23 and calcium-binding proteins should increase our knowledge of hair cell function at the molecular level.</p> <p>Conclusion</p> <p>The results of this study shed light on some protein networks in cochlear hair cells. Not only was a group of <it>de novo </it>genes closely associated with known deafness loci identified, but the data also indicate that the hair cell tip link interacts directly with calcium binding proteins. The OHC motor protein, prestin, also appears to be associated with electron transport proteins. These unanticipated results open potentially fruitful lines of investigation into the molecular basis of cochlear amplification.</p

Multitissue molecular, genomic, and developmental effects of the deepwater horizon oil spill on resident Gulf killifish (Fundulus grandis)

The Deepwater Horizon oil rig disaster resulted in crude oil contamination along the Gulf coast in sensitive estuaries. Toxicity from exposure to crude oil can affect populations of fish that live or breed in oiled habitats as seen following the Exxon Valdez oil spill. In an ongoing study of the effects of Deepwater Horizon crude oil on fish, Gulf killifish (Fundulus grandis) were collected from an oiled site (Grande Terre, LA) and two reference locations (coastal MS and AL) and monitored for measures of exposure to crude oil. Killifish collected from Grande Terre had divergent gene expression in the liver and gill tissue coincident with the arrival of contaminating oil and up-regulation of cytochrome P4501A (CYP1A) protein in gill, liver, intestine, and head kidney for over one year following peak landfall of oil (August 2011) compared to fish collected from reference sites. Furthermore, laboratory exposures of Gulf killifish embryos to field-collected sediments from Grande Terre and Barataria Bay, LA, also resulted in increased CYP1A and developmental abnormalities when exposed to sediments collected from oiled sites compared to exposure to sediments collected from a reference site. These data are predictive of population-level impacts in fish exposed to sediments from oiled locations along the Gulf of Mexico coast. © 2013 American Chemical Society

Inheritance of single copy nuclear genes (SCNGs) in artificial hybrids of Hesperocyparis arizonica x H. macrocarpa: Potential for utilization in the detection of hybridization in natural populations

Analyses were performed on 18 artificial hybrids from a cross of Hesperocyparis arizonica (male parent) x H. macrocarpa (female parent) using 9 single copy nuclear genes (SCNGs). Three SCNG were found to be informative: myb, 4CL and CnAIB2. Gene myb contained 5 variable sites, of which site 89 was homozygous (CC, TT) as was site 261 (GG, AA) and useful for the detection of hybridization. All 18 hybrids were heterozygous (CT and GA) at these 2 sites as predicted in hybrids. 4CL contained 8 variable sites, of which 1 site (591) was homozygous (TT, CC) and all 18 hybrids were heterozygous (TC) at this site as expected. CnAIP2 had two variable sites: 301 (AA, AC) and 554 (AG, AA). For site 301, 8 hybrids were AA, and 10 were AC as expected. For site 554, 10 hybrids were AA and 8 were AG, so neither would be useful for unequivocally identifying hybrids. The inheritance of variable sites for the three SCNGs followed simple co-occurrence. Examination of myb in the 18 hybrids revealed 2 cases of cross-over in the pollen gametes

Book Reviews

Reviews of the following books: Second Nature: An Environmental History of New England by Richard W. Judd; Hope and Fear in Margaret Chase Smith\u27s America: A Continuous Tangle by Gregory P. Gallant; The 2nd Maine Cavalry in the Civil War: A History and Roster by Ned Smith; Distilled in Maine: A History of Libations, Temperance and Craft Spirits by Kate McCarty; Bangor in World War II: From the Homefront to the Embattled Skies by David H. Bergquist; The Night the Sky Turned Red: The Story of the Great Portland Maine Fire of July 4th 1866, as told by Those Who Lived Through It by Allan M. Levinsk

A common founding clone with TP53 and PTEN mutations gives rise to a concurrent germ cell tumor and acute megakaryoblastic leukemia

We report the findings from a patient who presented with a concurrent mediastinal germ cell tumor (GCT) and acute myeloid leukemia (AML). Bone marrow pathology was consistent with a diagnosis of acute megakaryoblastic leukemia (AML M7), and biopsy of an anterior mediastinal mass was consistent with a nonseminomatous GCT. Prior studies have described associations between hematological malignancies, including AML M7 and nonseminomatous GCTs, and it was recently suggested that a common founding clone initiated both cancers. We performed enhanced exome sequencing on the GCT and the AML M7 from our patient to define the clonal relationship between the two cancers. We found that both samples contained somatic mutations in PTEN (C136R missense) and TP53 (R213 frameshift). The mutations in PTEN and TP53 were present at ∼100% variant allele frequency (VAF) in both tumors. In addition, we detected and validated five other shared somatic mutations. The copy-number analysis of the AML exome data revealed an amplification of Chromosome 12p. We also identified a heterozygous germline variant in FANCA (S858R), which is known to be associated with Fanconi anemia but is of uncertain significance here. In summary, our data not only support a common founding clone for these cancers but also suggest that a specific set of distinct genomic alterations (in PTEN and TP53) underlies the rare association between GCT and AML. This association is likely linked to the treatment resistance and extremely poor outcome of these patients. We cannot resolve the clonal evolution of these tumors given limitations of our data