Genetic stability of Campylobacter coli in patients with primary antibody deficiencies

In the Clinical Communication, Dion et al 1 reported that in patients with severe primary antibody deficiency (PAD), Campylobacter infection is a major cause (6.5%) of chronic or recurrent diarrhea. Moreover, by a molecular study performed in a subset of 18 strains from 5 patients with recurrent infections, they demonstrated that all strains were different, even when the episodes occurred closely over time. Thus, the authors hypothesized that reinfection would be more likely than persistent colonization, although colonization with multiple strains cannot be excluded. In a previous study, 2 our group showed that Campylobacter coli (C. coli) (6.7%) was the first cause of diarrhea in patients with symptomatic PAD with a positive stool culture, followed by Campylobacter jejuni (C. jejuni) (3.9%). Moreover, C. coli was also the most frequent isolate (5%) in patients with asymptomatic PAD, followed by C. jejuni (1.2%), whereas in immunocompetent individuals, C. jejuni is one of the most prevalent etiologic agents of gastroenteritis and C. coli has a low prevalence in diarrheal disease

Insights Into The Biological Role Of Ovothiol In Sea Urchins And Diatoms

Ovothiols are π-methyl-5-thiohistidines, first isolated and characterised from ovary, eggs and biological fluids of marine invertebrates. They are produced in large amounts by sea urchin eggs as a protection from the oxidative burst at fertilisation and from environmental cues during embryonic development. The position of the thiol group on the imidazole ring confers to these molecules unique chemical properties allowing them to play numerous biological roles in nature, as well as they are receiving an increasing interest as pharmacological compounds for a potential therapeutic use in humans. The key enzymes responsible for ovothiol biosynthesis, OvoA and OvoB, have been characterised revealing that its biosynthesis is much more widespread than previously thought. In this thesis, the physiological roles of ovothiol have been investigated in two marine organisms inhabiting coastal areas, sea urchins and diatoms, with the aim to highlight new biological functions and possible applications for human health. Through molecular and functional analyses of OvoA transcript and protein carried out during development of the Mediterranean sea urchin Paracentrotus lividus, this study has revealed that ovothiol biosynthesis is fundamental for the progression of a correct developmental program. In particular, the role of this metabolite is likely related to fundamental processes like cell cycle during early development, skeletogenesis and gut functionality in larval stages. On the other hand, molecular experiments performed in the centric diatom Skeletonema marinoi have revealed that OvoA transcription is modulated by light and is associated with reactive oxygen and nitrogen species variations, thus suggesting that ovothiol can be involved in the antioxidant response triggered by light in diatoms. These studies are relevant considering the possibility to use diatoms to produce high amounts of the compound necessary for applied research

Advances in the Hierarchical Emergent Behaviors (HEB) approach to autonomous vehicles

Widespread deployment of autonomous vehicles (AVs) presents formidable challenges in terms on handling scalability and complexity, particularly regarding vehicular reaction in the face of unforeseen corner cases. Hierarchical Emergent Behaviors (HEB) is a scalable architecture based on the concepts of emergent behaviors and hierarchical decomposition. It relies on a few simple but powerful rules to govern local vehicular interactions. Rather than requiring prescriptive programming of every possible scenario, HEB’s approach relies on global behaviors induced by the application of these local, well-understood rules. Our first two papers on HEB focused on a primal set of rules applied at the first hierarchical level. On the path to systematize a solid design methodology, this paper proposes additional rules for the second level, studies through simulations the resultant richer set of emergent behaviors, and discusses the communica-tion mechanisms between the different levels.Peer ReviewedPostprint (author's final draft

Marine 5-thiohistidines as protective molecules from skin damage

Introduction Marine environment is a great source of bioactive molecules, whose biological properties and applications are often used especially to prevent skin diseases and aging caused by UVA­exposure. Ovothiols are methyl­5­thiohistidines from marine invertebrates, bacteria, and microalgae, which protect cells from environmental stressors. Recently, we have shown that, ovothiol, isolated from sea urchin eggs, exerts anti­inflammatory and antioxidant activities on human endothelial cells, and exhibits antifibrotic effect in an in vivo model of liver fibrosis.info:eu-repo/semantics/publishedVersio

Emergent behaviors in the Internet of things: The ultimate ultra-large-scale system

To reach its potential, the Internet of Things (IoT) must break down the silos that limit applications' interoperability and hinder their manageability. Doing so leads to the building of ultra-large-scale systems (ULSS) in several areas, including autonomous vehicles, smart cities, and smart grids. The scope of ULSS is both large and complex. Thus, the authors propose Hierarchical Emergent Behaviors (HEB), a paradigm that builds on the concepts of emergent behavior and hierarchical organization. Rather than explicitly programming all possible decisions in the vast space of ULSS scenarios, HEB relies on the emergent behaviors induced by local rules at each level of the hierarchy. The authors discuss the modifications to classical IoT architectures required by HEB, as well as the new challenges. They also illustrate the HEB concepts in reference to autonomous vehicles. This use case paves the way to the discussion of new lines of research.Damian Roca work was supported by a Doctoral Scholarship provided by Fundación La Caixa. This work has been supported by the Spanish Government (Severo Ochoa grants SEV2015-0493) and by the Spanish Ministry of Science and Innovation (contracts TIN2015-65316-P).Peer ReviewedPostprint (author's final draft

Clinical management of patients with primary immunodeficiencies during the COVID-19 pandemic

Introduction: Patients affected by Inborn Errors of Immunity (IEI) represent a potential group-at-risk in the current COVID-19 pandemic. Studies on large and small cohorts of IEI reported a huge variability clinical manifestations associated to SARS-Cov-2, ranging from asymptomatic, mild, moderate/severe to death. A great impulse to improve remote assistance programs and to switch to home-based treatment to reduce mobility and face to face contacts has been implemented. Areas covered: The authors completed a comprehensive review of the literature by searching the PubMed database for studies on large and small cohorts and case reports of IEI patients with COVID-19, with the aim to provide useful information for their clinical management during the COVID-19 pandemic. Expert opinion: Surprisingly, a low number of IEI patients affected by SARS-Cov-2 were reported with a risk to die for COVID-19 overlapping that of the general population. The low number might be explained by the choice of most physicians to inform early in the pandemic about safety measures, to switch most of the IEI patients to home therapy and to remote assistance. The guidelines issued by the scientific societies and periodically updated, represent the best tool for the clinical management of IEI patients

Development and Initial Validation of a Questionnaire to Measure Health-Related Quality of Life of Adults with Common Variable Immune Deficiency: The CVID_QoL Questionnaire.

BACKGROUND: Generic health status quality of life (QoL) instruments have been used in patients with common variable immune deficiency (CVID). However, by their nature, these tools may over- or underestimate the impact of diseases on an individual's QoL. OBJECTIVE: The objective of this study was to develop and validate a questionnaire to measure specific-health-related QoL for adults with CVID (CVID_QoL). METHODS: The 32-item content of the CVID_QoL questionnaire was developed using focus groups and individual patient interviews. Validation studies included 118 adults with CVID who completed Short Form-36, Saint George Respiratory Questionnaire, General Health Questionnaire-12, and EuroQol-5D questionnaire in a single session. Principal component and factor analysis solutions identified 3 scores to be similar in number and content for each solution. Validation of 3 factor scores was performed by construct validity. Reproducibility, reliability, convergent validity, and discriminant validity were evaluated. Matrices consisting of correlations between the 32 items in the CVID_QOL were calculated. RESULTS: Factor analysis identified 3 dimensions: emotional functioning (EF), relational functioning (RF), and gastrointestinal and skin symptoms (GSS). The instrument had good internal consistency (Cronbach's alpha, min. 0.74 for GSS, max. 0.84 for RF, n = 118) and high reproducibility (intraclass correlation coefficient, min. 0.79 for RF, max 0.90 for EF, n = 27). EF and RF scores showed good convergent validity correlating with conceptually similar dimensions of other study scales. Acute and relapsing infections had a significant impact on EF and RF. CONCLUSIONS: This study provides evidence of the reliability and construct validity of the CVID_QoL to identify QoL issues in patients with CVID that may not be addressed by generic instruments

From Sea to Skin: Is There a Future for Natural Photoprotectants?

In the last few decades, the thinning of the ozone layer due to increased atmospheric pollution has exacerbated the negative effects of excessive exposure to solar ultraviolet radiation (UVR), and skin cancer has become a major public health concern. In order to prevent skin damage, public health advice mainly focuses on the use of sunscreens, along with wearing protective clothing and avoiding sun exposure during peak hours. Sunscreens present on the market are topical formulations that contain a number of different synthetic, organic, and inorganic UVR filters with different absorbance profiles, which, when combined, provide broad UVR spectrum protection. However, increased evidence suggests that some of these compounds cause subtle damage to marine ecosystems. One alternative may be the use of natural products that are produced in a wide range of marine species and are mainly thought to act as a defense against UVR-mediated damage. However, their potential for human photoprotection is largely under-investigated. In this review, attention has been placed on the molecular strategies adopted by marine organisms to counteract UVR-induced negative effects and we provide a broad portrayal of the recent literature concerning marine-derived natural products having potential as natural sunscreens/photoprotectants for human skin. Their chemical structure, UVR absorption properties, and their pleiotropic role as bioactive molecules are discussed. Most studies strongly suggest that these natural products could be promising for use in biocompatible sunscreens and may represent an alternative eco-friendly approach to protect humans against UV-induced skin damage