Running Economy while Running in Extreme Cushioning and Normal Cushioning Running Shoes

The purpose of the study was to determine if running economy was influenced by wearing maximal cushioning shoes vs. control (neutral cushioning) shoes. (Please see Abstract in text

The Trypanosoma cruzi enzyme TcGPXI is a glycosomal peroxidase and can be linked to trypanothione reduction by glutathione or tryparedoxin.

Trypanosoma cruzi glutathione-dependent peroxidase I (TcGPXI) can reduce fatty acid, phospholipid, and short chain organic hydroperoxides utilizing a novel redox cycle in which enzyme activity is linked to the reduction of trypanothione, a parasite-specific thiol, by glutathione. Here we show that TcGPXI activity can also be linked to trypanothione reduction by an alternative pathway involving the thioredoxin-like protein tryparedoxin. The presence of this new pathway was first detected using dialyzed soluble fractions of parasite extract. Tryparedoxin was identified as the intermediate molecule following purification, sequence analysis, antibody studies, and reconstitution of the redox cycle in vitro. The system can be readily saturated by trypanothione, the rate-limiting step being the interaction of trypanothione with the tryparedoxin. Both tryparedoxin and TcGPXI operate by a ping-pong mechanism. Overexpression of TcGPXI in transfected parasites confers increased resistance to exogenous hydroperoxides. TcGPXI contains a carboxyl-terminal tripeptide (ARI) that could act as a targeting signal for the glycosome, a kinetoplastid-specific organelle. Using immunofluorescence, tagged fluorescent proteins, and biochemical fractionation, we have demonstrated that TcGPXI is localized to both the glycosome and the cytosol. The ability of TcGPXI to use alternative electron donors may reflect their availability at the corresponding subcellular sites

A high-resolution mass spectrometry based proteomic dataset of human regulatory T cells

Regulatory T cells (Tregs) play a core role in maintaining immune tolerance, homeostasis, and host health. High-resolution analysis of the Treg proteome is required to identify enriched biological processes and pathways distinct to this important immune cell lineage. We present a comprehensive proteomic dataset of Tregs paired with conventional CD4+ (Conv CD4+) T cells in healthy individuals. Tregs and Conv CD4+ T cells were sorted to high purity using dual magnetic bead-based and flow cytometry-based methodologies. Proteins were trypsin-digested and analysed using label-free data-dependent acquisition mass spectrometry (DDA-MS) followed by label free quantitation (LFQ) proteomics analysis using MaxQuant software. Approximately 4,000 T cell proteins were identified with a 1% false discovery rate, of which approximately 2,800 proteins were consistently identified and quantified in all the samples. Finally, flow cytometry with a monoclonal antibody was used to validate the elevated abundance of the protein phosphatase CD148 in Tregs. This proteomic dataset serves as a reference point for future mechanistic and clinical T cell immunology and identifies receptors, processes, and pathways distinct to Tregs. Collectively, these data will lead to a better understanding of Treg immunophysiology and potentially reveal novel leads for therapeutics seeking Treg regulation

Management of Children With Chronic Wet Cough and Protracted Bacterial Bronchitis CHEST Guideline and Expert Panel Report

BACKGROUND: Wet or productive cough is common in children with chronic cough. We formulated recommendations based on systematic reviews related to the management of chronic wet cough in children (aged METHODS: We used the CHEST expert cough panel\u27s protocol for systematic reviews and the American College of Chest Physicians (CHEST) methodologic guidelines and GRADE framework (the Grading of Recommendations Assessment, Development and Evaluation). Data from the systematic reviews in conjunction with patients\u27 values and preferences and the clinical context were used to form recommendations. Delphi methodology was used to obtain consensus for the recommendations/suggestions made. RESULTS: Combining data from the systematic reviews, we found high-quality evidence in children aged 4 weeks\u27 duration) wet/productive cough that using appropriate antibiotics improves cough resolution, and further investigations (eg, flexible bronchoscopy, chest CT scans, immunity tests) should be undertaken when specific cough pointers (eg, digital clubbing) are present. When the wet cough does not improve following 4 weeks of antibiotic treatment, there is moderate-quality evidence that further investigations should be considered to look for an underlying disease. New recommendations include the recognition of the clinical diagnostic entity of protracted bacterial bronchitis. CONCLUSIONS: Compared with the 2006 Cough Guidelines, there is now high-quality evidence for some, but not all, aspects of the management of chronic wet cough in specialist settings. However, further studies (particularly in primary health) are required

“I Want Justice from People Who Did Bad Things to Children”: Experiences of Justice for Sex Trafficking Survivors

This research from the Butterfly Longitudinal Research Project focused on understanding the experiences and perceptions of justice and the justice system for 93 Cambodia participants (including 88 survivors of sex trafficking) as they navigated the legal system. Thirty-two of these survivors had experiences in court and provided details into their courtroom experiences, predominantly within Cambodia but also in the United States. The survivors’ experiences were diverse; however, the prevailing themes were: fear throughout their legal journeys; a low level of awareness and understanding of their legal experiences; and that NGO support was essential for these survivors to engage in the often complicated, lengthy and emotionally challenging legal processes. The recommendations generated from the results at the individual survivor level included: encouraging active participation to make informed decisions on their legal journey; survivors need compassionate support from all stakeholders throughout their legal journey; questioning needs to be appropriate, sensitive and age appropriate; and survivor safety needs to be central both in the courtroom and in the community, as perpetrators and their associates may be threatening. At a wider, societal level, the legal system should be accessible and encourage victim participation. Further, the outcomes of court should be adequate for survivors, in that they provide justice, restitution and deter future crimes; trust in the legal system needs to be developed; information and education about the legal system and legal rights should be promoted; and protection for survivors must be enhanced

Strategic Players for Identifying Optimal Social Network Intervention Subjects

We present a method whereby social network ties are used to identify behavioral leaders who are situated in the network such that these individuals are: 1) able to influence other individuals who are in need of and most receptive to intervention, thereby optimizing the impact of the intervention; and 2) not embedded with ties to individuals that are likely to be behaviorally antagonistic to the intervention or that would compromise the optimal impact of intervention. In this study we developed a method that we call Strategic Players, which is a solution for identifying a set of players who are close to a target subset of the network (i.e., the target group), and far away from the subset we wish to avoid (i.e. the avoid group), where the proximity to either the target or avoid group may be facilitated by network members who are in neither group (i.e. the neutral group). This solution seeks to maximize the diffusion of the behavior to the target group while minimizing contact and influence to the avoid group. We apply this method to two different social networks, and one simulated social network

Flora of Richmond National Battlefield Park, Virginia

An inventory of the vascular flora of nine of eleven units of Richmond National Battlefield Park was compiled from 1985 to 1987. Each site was visited during the growing season in two to four week intervals; plant species were identified and recorded in the field and/or collected for later study. A total of 761 different species were identified in the surveyed units, and 2487 individual records of species per particular park unit were noted. Twenty-three percent of the flora consists of exotic species, largely from Eurasia. Voucher specimens are housed in the herbaria of the University of Richmond and Virginia Commonwealth University

A Critical Examination of Contemporary Legal Issues Important to College Presidents

This discussion provides a highlight of the legal dimensions that college presidents must consider in providing oversight for college operations. Many of these issues are driven by the behaviors of campus constituents, but several key legal issues are also the result of federal legislation and involvement in higher education