The influence of the political environment and destination governance on sustainable tourism development: a study of Bled, Slovenia

In the context of sustainable tourism development, there are many studies about the exchange process between residents and tourism, yet this issue is practically unexplored with respect to the political environment of tourism. Therefore, this paper introduces and posits that the political environment is a necessary enabler for implementing sustainable tourism. The authors extend the established three-pillar sustainability concept by adding in the political dimension. Then they surveyed how residents' positive and negative perceptions of tourism impacts determine their satisfaction with life in the tourism destination and thus their support for tourism in their community. The model was empirically tested within the context of the long-established Alpine destination of Bled in Slovenia. The findings confirm the importance of the political environment and question the sustainability of Bled's tourism development. It is suggested that the community has relatively weak destination governance due to the underdeveloped political environment. The survey expands and deepens the tourism sustainability debate by adding in the political environment and how it relates to the emerging growth of research on destination governance. The proposed model can be adapted and applied to any destination in order to improve its governance, including the implementation of sustainable tourism development

Resident perceptions and responses to tourism: individual vs community level impacts

This paper reviews resident tourism attitude research through the lens of the individual- and community-level perceived impacts of and responses to tourism. It explores how perceived impacts of tourism and responses to tourism development have been conceptualised and measured in the existing resident attitudes models published between 1990 and 2020. Three categories of variables were identified and used: antecedent variables, tourism impact variables, and dependent variables. The latter three categories are used to discuss the research topic from the lenses of improvements in measurement instruments. Finally, the paper suggests rethinking the overall conceptualisation of residents’ perceptions of and reactions to tourism – it proposes future research directions to distinguish between individual-level and community-level effects and reactions

Analiza dizajna LCLC rezonantnog invertera za dvostupanjsko dvofazno napajanje

This paper deals with the design analysis and synthesis of power resonant inverter with sinusoidal output voltage for sensitive loads. The proposed filter must be capable of removing higher harmonic components from the supplying voltage to reach a harmonic distortion of roughly 5% in the whole range of the load (0 − 100%). The inverter can be supplied from either single-phase voltage inverter in full- or half- bridge connection, or from simple DC/DC buck converter. Non-symmetrical control causes higher harmonic content, both odd and even. Simulation and experimental results based on designed parameters and subsequently obtained from Matlab and OrCad models confirm good quality of output quantities, voltage and current.Tema je ovog članka analiza dizajna i sinteza učinskog rezonantnog invertera sa sinisuidalnim izlaznim naponom za osjetljive terete. Predloženi filtar mora moći filtrirati više harmonike ulaznog napona kako bi distorzija harmonika bila oko 5% u čitavom radnom području (0−100%). Inverter se može napajati ili iz jednofaznog naponskog invertera u mosnom ili uzrokuje pojavu viših harmonika u signalu, kako parnih tako i neparnih. Simulacijski i eksperimentalni rezultati temeljeni na sintetiziranim parametrima dobivenim od modela napravljenih u programskim paketima Matlab i OrCad potvr.uju dobru kvalitetu izlaznih veličina napona i struje

Poboljšana elektromagnetska kompatibilnost (EMC) sklopnog energetskog pretvarača sa slučajnom modulacijom

This paper surveys some analytical and experimental results concerning different randomized modulation strategies in switched-mode power converters (SMPCs). After a short review of practical experiences within the literature it presents the benefits of several randomized schemes for power converters (i.e. reduced electromagnetic interference - EMI, and lower acoustic noise). It also introduces the mathematical background for dealing with randomized modulation within the medium-frequency range: power spectrum density (PSD). Finally, the EMI measurements confirm the improved EMC performances of the randomized boost rectifier, as also in the DC-DC buck converter.Ovaj članak istražuje analitičke i eksperimentalne rezultate različitih slučajnih strategija modulacije u sklopnim energetskim pretvaračima (SMPCs). Nakon kratkog pregleda praktičnih iskustava iz literature, predstavljene su prednosti nekoliko slučajnih shema za energetske pretvarače (smanjena elektromagnetska interferencija - EMI, i niži akustični šum). Također, uvedena je matematička podloga za rad sa slučajnom modulacijom u području srednjih frekvencija: spektralna gustoća snage (PSD). Konačno, EMI mjerenja potvrđuju poboljšanja EMC performansi slučajnih uzlaznih ispravljača, kao i DC-DC silaznih pretvarača

Conservation of Affinity Rather Than Sequence Underlies a Dynamic Evolution of the Motif-Mediated p53/MDM2 Interaction in Ray-Finned Fishes.

The transcription factor and cell cycle regulator p53 is marked for degradation by the ubiquitin ligase MDM2. The interaction between these 2 proteins is mediated by a conserved binding motif in the disordered p53 transactivation domain (p53TAD) and the folded SWIB domain in MDM2. The conserved motif in p53TAD from zebrafish displays a 20-fold weaker interaction with MDM2, compared to the interaction in human and chicken. To investigate this apparent difference, we tracked the molecular evolution of the p53TAD/MDM2 interaction among ray-finned fishes (Actinopterygii), the largest vertebrate clade. Intriguingly, phylogenetic analyses, ancestral sequence reconstructions, and binding experiments showed that different loss-of-affinity changes in the canonical binding motif within p53TAD have occurred repeatedly and convergently in different fish lineages, resulting in relatively low extant affinities (KD = 0.5 to 5 μM). However, for 11 different fish p53TAD/MDM2 interactions, nonconserved regions flanking the canonical motif increased the affinity 4- to 73-fold to be on par with the human interaction. Our findings suggest that compensating changes at conserved and nonconserved positions within the motif, as well as in flanking regions of low conservation, underlie a stabilizing selection of functional affinity in the p53TAD/MDM2 interaction. Such interplay complicates bioinformatic prediction of binding and calls for experimental validation. Motif-mediated protein-protein interactions involving short binding motifs and folded interaction domains are very common across multicellular life. It is likely that the evolution of affinity in motif-mediated interactions often involves an interplay between specific interactions made by conserved motif residues and nonspecific interactions by nonconserved disordered regions

Conservation of Affinity Rather Than Sequence Underlies a Dynamic Evolution of the Motif-Mediated p53/MDM2 Interaction in Ray-Finned Fishes

The transcription factor and cell cycle regulator p53 is marked for degradation by the ubiquitin ligase MDM2. The interaction between these 2 proteins is mediated by a conserved binding motif in the disordered p53 transactivation domain (p53TAD) and the folded SWIB domain in MDM2. The conserved motif in p53TAD from zebrafish displays a 20-fold weaker interaction with MDM2, compared to the interaction in human and chicken. To investigate this apparent difference, we tracked the molecular evolution of the p53TAD/MDM2 interaction among ray-finned fishes (Actinopterygii), the largest vertebrate clade. Intriguingly, phylogenetic analyses, ancestral sequence reconstructions, and binding experiments showed that different loss-of-affinity changes in the canonical binding motif within p53TAD have occurred repeatedly and convergently in different fish lineages, resulting in relatively low extant affinities (KD = 0.5 to 5 mu M). However, for 11 different fish p53TAD/MDM2 interactions, nonconserved regions flanking the canonical motif increased the affinity 4- to 73-fold to be on par with the human interaction. Our findings suggest that compensating changes at conserved and nonconserved positions within the motif, as well as in flanking regions of low conservation, underlie a stabilizing selection of "functional affinity" in the p53TAD/MDM2 interaction. Such interplay complicates bioinformatic prediction of binding and calls for experimental validation. Motif-mediated protein-protein interactions involving short binding motifs and folded interaction domains are very common across multicellular life. It is likely that the evolution of affinity in motif-mediated interactions often involves an interplay between specific interactions made by conserved motif residues and nonspecific interactions by nonconserved disordered regions

Eco-labelling of marine recreation services: the case of Blue Flag price premium in Croatia

Eco-labels or eco-certifications communicate to the public and tourists that a business is employing one or more environmentally friendly practices. A successful label provides incentives for industry to participate by allowing for further product differentiation, leading to increased price premia. This paper examines whether the Blue Flag eco-label has a positive influence on prices of tourism services associated with marinas and beaches in coastal Croatia. For this purpose, panel data regression models are employed, and they explicitly address endogeneity of the eco-label variable arising from selection of firms adopting the label. Treatment effects and instrumental variables specifications are estimated, and results suggest positive impacts of the Blue Flag label on marina slip rental, sailboat charter, and hotel services, with price premia ranging between 18% and 72%. These results may be of use to businesses, governmental, and non-governmental organisations interested in pursuing such certification