Efficiency of blocking of non-specific interaction of different proteins by BSA adsorbed on hydrophobic and hydrophilic surfaces

AcceptéInternational audienc

Inactivation Of E. Coli Mediated By High Surface Area Cuo Accelerated By Light Irradiation >360 Nm

CuO powders with different specific surface areas are reported hereby to inactivate E. coli in aqueous solution in the dark under visible light irradiation λ > 360 nm. The inactivation of E. coli mediated by the CuO suspensions was investigated as a function of the solution parameters: specific surface area of the Cu-oxides (40-77 m 2/g), amount of CuO, light intensity and fate of the Cu 1+-ion within the inactivation process. The specific surface area of the CuO was observed to play an important role during the E. coli inactivation kinetics. The light induced inactivation of E. coli in CuO suspensions (1 g/L) was complete within 4 h. The cytotoxicity of E. coli when using CuO (77 m 2/g) was found for CuO concentrations as low as 0.2 g/L. A reaction mechanism is suggested for the Fenton-like reactions due to the Cu-ions/CuO action and the reactive oxygen species (ROS) generated in solution. These highly oxidative radicals decompose Orange II and methylene blue (MB) dyes in aqueous solution of CuO. The CuO in contact with the bacterial suspension shows a change in its surface oxidation state from Cu 2+ to Cu 1+. The outermost layer of the catalyst (5-7 nm) becomes mainly Cu 2O (80%) and CuO (20%) as observed by X-ray photoelectron spectroscopy (XPS). A shift of the Cu 2p 3/2 peak from the initial position at 933.6-932.6 eV upon contact of the E. coli with CuO was observed concomitant with the disappearance of the Cu 2+ shake-up satellite lines at 942.3 and 962.2 eV. The XPS surface composition of copper catalyst is reported at different stages of E. coli inactivation and it was observed that the reduced copper oxide remains stable during the 4 h needed to inactivate the E. coli suspension. © 2008 Elsevier B.V. All rights reserved.1991105111D.K. Karlin, Y. Gulneth, In Progress in Inorganic Chemistry, vol. 35, Lippard, Ed., 1987, pp. 220-237Tolman, B.W., (1997) Acc. Chem. Res., 30, pp. 227-240Bandara, J., Guasaquillo, I., Bowen, P., Soare, L., Jardim, F.W., Kiwi, J., (2005) Langmuir, 21, pp. 8554-8559Bandara, J., Kiwi, J., Pulgarin, C., Peringer, P., Pajonk, G.-M., Elalui, A., Albers, P., (1996) Environ. Sci. Technol., 30, pp. 1261-1267Li, D., Yuranova, T., Kiwi, J., (2004) Water Res., 38, pp. 3541-3550Oppenlaender, Th., (2003) Photochemical Purification of Water and Air, , Wiley-VCH, Weinheim, GermanyGak, Y., Nadtochenko, V., Kiwi, J., (1998) J. Photochem. Photobiol. A., 116, pp. 57-62Carnes, L.C., Stipp, J., Klabunde, J., Bonevich, J., (2002) Langmuir, 18, pp. 1352-1358Wang, W., Zhan, X., Wang, Y., Liu, Y., Zheng, G., Wang, G., (2002) Mater. Res. Bull., 37, pp. 1092-1100Sadana, A., Katzer, J., (1974) J. Catal., 35, pp. 140-152Hai-Yan, D., Yu-Ling, C., Jing-Kui, L., Si-Shen, X.J., (1993) Mater. Sci., 28, pp. 5176-5178Walsh, D., Arcelli, T., Ikoma, J., Tanaka, J., Mann, S., (2003) Nat. Maters, 2, pp. 386-388Yokota, T., Kubota, Y., Takahata, Y., Katsuyama, T., Matsuda, Y., (2004) J. Chem. Eng. Jpn, 37, pp. 238-244(2002) Drug Ther. Bull., 40, pp. 67-69Bader, H., Sturzenegger, V., Hoigné, (1988) J. Wat. Res., 22, pp. 1109-1115Hulanicki, A., Krawczyk, T.K.V., Lewenstam, A., (1984) Anal. Chim. Acta, 158, pp. 343-355Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C., Yolken, R.H., (1995) Manual of Clinical Microbiology. sixth edition, , American Society of Microbiology, Washington, D.CCooney, T.E., (1995) Infect. Control. Hosp. Epidemiol., 16, pp. 444-450Bacsa, R., Kiwi, J., Ohno, T., Albers, P., Nadtochenko, V., (2005) J. Phys. Chem. B., 109, pp. 5994-6003. , (and references therein)Sunada, K., Watanabe, T., Hashimoto, K., (2003) Environ. Sci. Technol., 37, pp. 4785-4789Hardee, K., Bard, A., (1977) J. Electrochem. Soc., 124, p. 215Hardee, K., Bard, A., (1977) J. Electrochem. Soc., 124, pp. 215-224Goldstein, S., Czapski, G., Meyerstein, D., (1990) J. Am. Chem. Soc., 112, pp. 6489-6493Bielski, J.B., Cabelli, D., Arudi, R., Ross, A., (1985) J. Phys. Chem. Ref. Data, 14, pp. 1041-1061Petasne, R.G., Zika, R.G., (1997) Mar. Chem., 56, pp. 215-225Kieber, R.J., George, R.H., (1995) Estuarine, Coastal Shelf Sci., 40, pp. 495-503Cooper, W.J., Lean, D.R.S., (1989) Environ. Sci. Technol., 23, pp. 1425-1428Jardim, W.F., Soldá, M.I., Gimenez, S.M., (1986) Sci. Total Environ., 58, pp. 47-54Weiss, J., (1935) Naturwissenchaften, 23, pp. 64-67Letelier, M.E., Lepe, A., Faundez, M., Salazar, J., Marin, R., Aracena, P., Speisky, H., (2005) Chem.-Biol. Interact., 151, pp. 71-82Takeshi, N., Insook, M., Noriyuki, S., Takakiro, I., (1997) J. Biol. Chem., 272, pp. 23037-2304

Data modeling for Tools and Technologies for the Analysis and Synthesis of NANOstructures (TASNANO) project

The main aim of TASNANO project is to focus on the development of a web-based system that allows cooperative work between partners. Even if some commercial tools become available, the specificities of nanotechnology applications suggest implementing an ad-hoc tool. This approach permits to update information every moment, so that not only the latest versions of the documents are always available, but also the raw experimental data are shared in a protected environment. Organizing big amount of data begins with modelling of conceptual schemes, which individualizes entities involved into the project and links between them. The rules\u2019 application of logic modeling leads to the production of a logic scheme. The model allows the data base design, which can be enlarged and enriched preserving coherence and avoiding redundancy. Data and information can be directly treated on the web in accordance with latest modifications