55 research outputs found
Characterization of genetically modified mice for phosphoglycerate mutase, a vitally-essential enzyme in glycolysis
Models de ratolí; Glucòlisi; Diabetis mellitusModelos de ratón; Glucólisis; Diabetes mellitusMouse models; Glycolysis; Diabetes mellitusGlycolytic metabolism is closely involved in physiological homeostasis and pathophysiological states. Among glycolytic enzymes, phosphoglycerate mutase (PGAM) has been reported to exert certain physiological role in vitro, whereas its impact on glucose metabolism in vivo remains unclear. Here, we report the characterization of Pgam1 knockout mice. We observed that homozygous knockout mice of Pgam1 were embryonic lethal. Although we previously reported that both PGAM-1 and -2 affect global glycolytic profile of cancers in vitro, in vivo glucose parameters were less affected both in the heterozygous knockout of Pgam1 and in Pgam2 transgenic mice. Thus, the impact of PGAM on in vivo glucose metabolism is rather complex than expected before.This work was supported in part by grants from the Japan Society for the Promotion of Science (Grants No. 26310103 to HK and No. 15K19283 to HK), and by the Japan Agency for Medical Research and Development (AMED), Core Research for Evolutional Science and Technology (CREST JP17gm0610002h0306 to HK). HK; Hiroshi Kondoh
Upregulated Expression of Activation-Induced Cytidine Deaminase in Ocular Adnexal Marginal Zone Lymphoma with IgG4-Positive Cells
Immunoglobulin G4-related disease (IgG4-RD) is a systemic disorder characterized by tissue fibrosis and intense lymphoplasmacytic infiltration, causing progressive organ dysfunction. Activation-induced cytidine deaminase (AID), a deaminase normally expressed in activated B-cells in germinal centers, edits ribonucleotides to induce somatic hypermutation and class switching of immunoglobulin. While AID expression is strictly controlled under physiological conditions, chronic inflammation has been noted to induce its upregulation to propel oncogenesis. We examined AID expression in IgG4-related ophthalmic disease (IgG4-ROD; n = 16), marginal zone lymphoma with IgG4-positive cells (IgG4+ MZL; n = 11), and marginal zone lymphoma without IgG4-positive cells (IgG4- MZL; n = 12) of ocular adnexa using immunohistochemical staining. Immunohistochemistry revealed significantly higher AID-intensity index in IgG4-ROD and IgG4+ MZL than IgG4- MZL (p < 0.001 and = 0.001, respectively). The present results suggest that IgG4-RD has several specific causes of AID up-regulation in addition to inflammation, and AID may be a driver of oncogenesis in IgG4-ROD to IgG4+ MZL
Endoscopic resection of pleomorphic adenoma
Background : An accessory parotid gland (APG) is a common anatomical structure that occurs in 10%–56% of individuals. Pleomorphic adenomas are the most common benign tumors of the APG, and their ideal treatment is surgical excision, although there is a risk for aesthetic disorders and facial nerve damage due to the site of origin. Moreover, despite being benign, these tumors are known to recur. Therefore, it is necessary to achieve both reliable excision and avoidance of facial nerve damage. Case presentation : We report a case of a 49-year-old Japanese man with a mass in his left cheek. The lesion was diagnosed as a benign salivary gland tumor derived from the APG by computed tomography imaging, magnetic resonance imaging and fine needle aspiration cytology. We resected the tumor using modified high submandibular incision under the endoscopic-assisted field of view. Discussion and Conclusions : The tumor was less invasive and reliably resected using an endoscope. In surgical treatment, the endoscopic-assisted technique is very useful to achieve complete tumor resection and prevent relapse while avoiding serious complications due to surgical procedures
Streptococcus anginosus のプロリルトリペプチジルペプチダーゼの産生と酵素性状
Streptococcus anginosus is considered to be implicated in the etiology of oral infectious diseases as well as abscess formation in various body sites. We investigated the production and the enzymatic properties of PTP of S. anginosus NCTC 10713. This enzyme was found only in cell extract and active on tripeptide substrates containing proline residue at P1 position, particularly H−Ala−Ala−Pro−p−nitroanilide. The enzyme was produced by all 8 species of tested streptococci, indicating occurrence of this enzyme is rather ubiquitous within streptococci. This PTP was purified to homogeneity from the cell extract by the procedures including ammonium sulfate precipitation, chromatography, gel filtration and electrophoresis. The enzyme was inhibited by serine enzyme inhibitors and chelating reagents, indicating this PTP is a serine metalloenzyme with a molecular mass of 66 kDa. The enzyme was active against H−Ala−Ala−Pro−p−nitroanilide and H−Ala−Phe−Pro−p−nitroanilide in neutral pH solutions. The activity was completely lost by heating at 50°C for 10min
Single electron yields from semileptonic charm and bottom hadron decays in AuAu collisions at GeV
The PHENIX Collaboration at the Relativistic Heavy Ion Collider has measured
open heavy-flavor production in minimum bias AuAu collisions at
GeV via the yields of electrons from semileptonic decays
of charm and bottom hadrons. Previous heavy-flavor electron measurements
indicated substantial modification in the momentum distribution of the parent
heavy quarks due to the quark-gluon plasma created in these collisions. For the
first time, using the PHENIX silicon vertex detector to measure precision
displaced tracking, the relative contributions from charm and bottom hadrons to
these electrons as a function of transverse momentum are measured in AuAu
collisions. We compare the fraction of electrons from bottom hadrons to
previously published results extracted from electron-hadron correlations in
collisions at GeV and find the fractions to be
similar within the large uncertainties on both measurements for
GeV/. We use the bottom electron fractions in AuAu and along
with the previously measured heavy flavor electron to calculate the
for electrons from charm and bottom hadron decays separately. We find
that electrons from bottom hadron decays are less suppressed than those from
charm for the region GeV/.Comment: 432 authors, 33 pages, 23 figures, 2 tables, 2011 data. v2 is version
accepted for publication by Phys. Rev. C. Plain text data tables for the
points plotted in figures for this and previous PHENIX publications are (or
will be) publicly available at http://www.phenix.bnl.gov/papers.htm
Measurement of higher cumulants of net-charge multiplicity distributions in AuAu collisions at GeV
We report the measurement of cumulants () of the net-charge
distributions measured within pseudorapidity () in AuAu
collisions at GeV with the PHENIX experiment at the
Relativistic Heavy Ion Collider. The ratios of cumulants (e.g. ,
) of the net-charge distributions, which can be related to volume
independent susceptibility ratios, are studied as a function of centrality and
energy. These quantities are important to understand the quantum-chromodynamics
phase diagram and possible existence of a critical end point. The measured
values are very well described by expectation from negative binomial
distributions. We do not observe any nonmonotonic behavior in the ratios of the
cumulants as a function of collision energy. The measured values of and can be directly compared to lattice
quantum-chromodynamics calculations and thus allow extraction of both the
chemical freeze-out temperature and the baryon chemical potential at each
center-of-mass energy.Comment: 512 authors, 8 pages, 4 figures, 1 table. v2 is version accepted for
publication in Phys. Rev. C as a Rapid Communication. Plain text data tables
for the points plotted in figures for this and previous PHENIX publications
are (or will be) publicly available at http://www.phenix.bnl.gov/papers.htm
Transverse energy production and charged-particle multiplicity at midrapidity in various systems from to 200 GeV
Measurements of midrapidity charged particle multiplicity distributions,
, and midrapidity transverse-energy distributions,
, are presented for a variety of collision systems and energies.
Included are distributions for AuAu collisions at ,
130, 62.4, 39, 27, 19.6, 14.5, and 7.7 GeV, CuCu collisions at
and 62.4 GeV, CuAu collisions at
GeV, UU collisions at GeV,
Au collisions at GeV, HeAu collisions at
GeV, and collisions at
GeV. Centrality-dependent distributions at midrapidity are presented in terms
of the number of nucleon participants, , and the number of
constituent quark participants, . For all collisions
down to GeV, it is observed that the midrapidity data
are better described by scaling with than scaling with . Also presented are estimates of the Bjorken energy density,
, and the ratio of to ,
the latter of which is seen to be constant as a function of centrality for all
systems.Comment: 706 authors, 32 pages, 20 figures, 34 tables, 2004, 2005, 2008, 2010,
2011, and 2012 data. v2 is version accepted for publication in Phys. Rev.
Measurement of jet-medium interactions via direct photon-hadron correlations in AuAu and Au collisions at GeV
We present direct photon-hadron correlations in 200 GeV/A AuAu, Au
and collisions, for direct photon from 5--12 GeV/, collected
by the PHENIX Collaboration in the years from 2006 to 2011. We observe no
significant modification of jet fragmentation in Au collisions,
indicating that cold nuclear matter effects are small or absent. Hadrons
carrying a large fraction of the quark's momentum are suppressed in AuAu
compared to and Au. As the momentum fraction decreases, the
yield of hadrons in AuAu increases to an excess over the yield in
collisions. The excess is at large angles and at low hadron and is most
pronounced for hadrons associated with lower momentum direct photons.
Comparison to theoretical calculations suggests that the hadron excess arises
from medium response to energy deposited by jets.Comment: 578 authors from 80 institutions, 11 pages, 7 figures, data from
2007, 2008, 2010, and 2011. v2 is version accepted for publication in
Physical Review C. Plain text data tables for the points plotted in figures
for this and previous PHENIX publications are (or will be) publicly available
at http://www.phenix.bnl.gov/papers.htm
The whole blood transcriptional regulation landscape in 465 COVID-19 infected samples from Japan COVID-19 Task Force
「コロナ制圧タスクフォース」COVID-19患者由来の血液細胞における遺伝子発現の網羅的解析 --重症度に応じた遺伝子発現の変化には、ヒトゲノム配列の個人差が影響する--. 京都大学プレスリリース. 2022-08-23.Coronavirus disease 2019 (COVID-19) is a recently-emerged infectious disease that has caused millions of deaths, where comprehensive understanding of disease mechanisms is still unestablished. In particular, studies of gene expression dynamics and regulation landscape in COVID-19 infected individuals are limited. Here, we report on a thorough analysis of whole blood RNA-seq data from 465 genotyped samples from the Japan COVID-19 Task Force, including 359 severe and 106 non-severe COVID-19 cases. We discover 1169 putative causal expression quantitative trait loci (eQTLs) including 34 possible colocalizations with biobank fine-mapping results of hematopoietic traits in a Japanese population, 1549 putative causal splice QTLs (sQTLs; e.g. two independent sQTLs at TOR1AIP1), as well as biologically interpretable trans-eQTL examples (e.g., REST and STING1), all fine-mapped at single variant resolution. We perform differential gene expression analysis to elucidate 198 genes with increased expression in severe COVID-19 cases and enriched for innate immune-related functions. Finally, we evaluate the limited but non-zero effect of COVID-19 phenotype on eQTL discovery, and highlight the presence of COVID-19 severity-interaction eQTLs (ieQTLs; e.g., CLEC4C and MYBL2). Our study provides a comprehensive catalog of whole blood regulatory variants in Japanese, as well as a reference for transcriptional landscapes in response to COVID-19 infection
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