Jack Goldstein in Los Angeles

This paper reexamines Jack Goldstein as one of the key but under recognized figures of what came to be known as the postmodern period in American art. Often described as bridging the gap between conceptualism and pop art, his work explores visual strategies of representation through the lens of art and mass media and how they inform our understanding of the world around us. Goldstein\u27s interest in representation is outlined by the cultural practices and aesthetic priorities of Los Angeles (where he began making art) of the late 1960s and 1970s and by extension, Hollywood

Immune DNA signature of T-cell infiltration in breast tumor exomes.

Tumor infiltrating lymphocytes (TILs) have been associated with favorable prognosis in multiple tumor types. The Cancer Genome Atlas (TCGA) represents the largest collection of cancer molecular data, but lacks detailed information about the immune environment. Here, we show that exome reads mapping to the complementarity-determining-region 3 (CDR3) of mature T-cell receptor beta (TCRB) can be used as an immune DNA (iDNA) signature. Specifically, we propose a method to identify CDR3 reads in a breast tumor exome and validate it using deep TCRB sequencing. In 1,078 TCGA breast cancer exomes, the fraction of CDR3 reads was associated with TILs fraction, tumor purity, adaptive immunity gene expression signatures and improved survival in Her2+ patients. Only 2/839 TCRB clonotypes were shared between patients and none associated with a specific HLA allele or somatic driver mutations. The iDNA biomarker enriches the comprehensive dataset collected through TCGA, revealing associations with other molecular features and clinical outcomes

Supramolecular self-assembly of gold rectangles

Master'sMASTER OF SCIENC

Mechanisms of CFTR Functional Variants That Impair Regulated Bicarbonate Permeation and Increase Risk for Pancreatitis but Not for Cystic Fibrosis

CFTR is a dynamically regulated anion channel. Intracellular WNK1-SPAK activation causes CFTR to change permeability and conductance characteristics from a chloride-preferring to bicarbonate-preferring channel through unknown mechanisms. Two severe CFTR mutations (CFTRsev) cause complete loss of CFTR function and result in cystic fibrosis (CF), a severe genetic disorder affecting sweat glands, nasal sinuses, lungs, pancreas, liver, intestines, and male reproductive system. We hypothesize that those CFTR mutations that disrupt the WNK1-SPAK activation mechanisms cause a selective, bicarbonate defect in channel function (CFTRBD) affecting organs that utilize CFTR for bicarbonate secretion (e.g. the pancreas, nasal sinus, vas deferens) but do not cause typical CF. To understand the structural and functional requirements of the CFTR bicarbonate-preferring channel, we (a) screened 984 well-phenotyped pancreatitis cases for candidate CFTRBD mutations from among 81 previously described CFTR variants; (b) conducted electrophysiology studies on clones of variants found in pancreatitis but not CF; (c) computationally constructed a new, complete structural model of CFTR for molecular dynamics simulation of wild-type and mutant variants; and (d) tested the newly defined CFTRBD variants for disease in non-pancreas organs utilizing CFTR for bicarbonate secretion. Nine variants (CFTR R74Q, R75Q, R117H, R170H, L967S, L997F, D1152H, S1235R, and D1270N) not associated with typical CF were associated with pancreatitis (OR 1.5, p = 0.002). Clones expressed in HEK 293T cells had normal chloride but not bicarbonate permeability and conductance with WNK1-SPAK activation. Molecular dynamics simulations suggest physical restriction of the CFTR channel and altered dynamic channel regulation. Comparing pancreatitis patients and controls, CFTRBD increased risk for rhinosinusitis (OR 2.3, p<0.005) and male infertility (OR 395, p<<0.0001). WNK1-SPAK pathway-activated increases in CFTR bicarbonate permeability are altered by CFTRBD variants through multiple mechanisms. CFTRBD variants are associated with clinically significant disorders of the pancreas, sinuses, and male reproductive system.Fil: LaRusch, Jessica. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Jung, Jinsei. Yonsei University College of Medicine; Corea del SurFil: General, Ignacio. University of Pittsburgh; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lewis, Michele D.. Mayo Clinic. Division of Gastroenterology and Hepatology; Estados UnidosFil: Park, Hyun Woo. Yonsei University College of Medicine; Corea del SurFil: Brand, Randall E.. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Gelrud, Andres. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Anderson, Michelle A.. University of Michigan; Estados UnidosFil: Banks, Peter A.. Brigham and Women’s Hospital. Division of Gastroenterology; Estados UnidosFil: Conwell, Darwin. Brigham and Women’s Hospital. Division of Gastroenterology; Estados UnidosFil: Lawrence, Christopher. Medical University of South Carolina; Estados UnidosFil: Romagnuolo, Joseph. Medical University of South Carolina; Estados UnidosFil: Baillie, John. University of Duke; Estados UnidosFil: Alkaade, Samer. St. Louis University. School of Medicine; Estados UnidosFil: Cote, Gregory. Indiana University; Estados UnidosFil: Gardner, Timothy B.. Dartmouth-Hitchcock Medical Center; Estados UnidosFil: Amann, Stephen T.. North Mississippi Medical Center; Estados UnidosFil: Slivka, Adam. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Sandhu, Bimaljit. Virginia Commonwealth University Medical Center; Estados UnidosFil: Aloe, Amy. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Kienholz, Michelle L.. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Yadav, Dhiraj. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Barmada, M. Michael. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Bahar, Ivet. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: Lee, Min Goo. Yonsei University College of Medicine; Corea del SurFil: Whitcomb, David C.. Univeristy of Pittsburgh. School of Medicine; Estados UnidosFil: North American Pancreatitis Study Group. No especifica

Northern Orange Latino community, Orange County, North Carolina : an action-oriented community diagnosis : findings and next steps of action

From September 2004 – April 2005, a six-member graduate student team from the Department of Health Behavior and Health Education at the University of North Carolina at Chapel Hill conducted an Action Oriented Community Diagnosis (AOCD) with the Latino Community (LC) of Northern Orange County (NOC). An AOCD examines the quality of life, community capacity, and strengths and needs of a community. Two preceptors from the Orange County Public Health Department mentored the student team throughout this process. The students interviewed 67 community members and service providers in both individual and group interview settings. Additional information was collected using secondary data sources and participant observations by the student team. The students presented the results at a community forum held in Hillsborough, North Carolina on April 9, 2005. Major topics that were discussed and addressed at the forum include transportation, adult education, linguistically and culturally competent service providers, a central location for information, and dental care. Below, a summary of the themes and the key action steps that were laid out at the community forum are listed (note Appendix F): There is a lack of public transportation in Northern Orange County. 1. Develop bilingual pamphlets about existing public transportation in Northern Orange County and distribute them to churches and social service agencies serving Latinos. 2. The Department of Transportation will hold a forum to inform Latinos of the extended bus routes that are scheduled to take effect in January 2006. 3. A bilingual community member will attend the Department of Transportation (DOT) board meetings. The Latino community needs more dental services that are affordable. 1. Hold dental screening and cleaning for preschoolers. 2. Publicize this and other events through a network of Promotores de Salud (community members who have received training in health education) as well as churches serving Latinos. 3. Latino community members will help the dental clinic to demonstrate the need for more dental services that are affordable. There is a need for a central location in Northern Orange County that provides the Latino community with information about existing social and health services. 1. Establish a planning committee. 2. Speak to libraries, schools, churches, and county commissioners about office space. 3. Coordinate a petition from the community and service providers. There is a need for more service providers in Northern Orange County with linguistic and cultural competency. 1. Invite the following agencies to learn how to recruit bilingual service providers: Orange County Health Department Medical Clinic in Hillsborough. Orange County Health Department Dental Clinic in Hillsborough. Orange County Department of Social Services. Day cares. Schools (parent/teacher conferences). Police. Courts. 2. Plan and hold job fairs. 3. Share resources and bilingual personnel between towns and counties. 4. Increase English as a Second Language (ESL) educational opportunities. 5. Offer free Spanish classes to personnel in agencies serving a large number of Latinos. There is a need for more adult education in Northern Orange County, such as ESL classes and job skills training. 1. Ask about possible locations for ESL classes. 2. Ask the following people and places for information and help: Teachers and schools. Social service agencies. Day cares. County politiciansMaster of Public Healt