Aquatic Ecosystem Response to Storm Water Abatement Measures in the Ravines of the GVSU Allendale Campus: Establishment of Base-line Biological Condition

The ravine tributary streams surrounding Grand Valley State Universities Allendale campus represent unique and understudied ecosystems, worthy of significant restoration efforts and of long-lasting protection. They are variously affected by storm water runoff, representing a spectrum from severely impacted to relatively pristine. Quantitative macroinvertebrate samples taken from six streams in late June 2007, indicated that insect diversity was positively correlated to ammonium (p=0.057), while total abundance was negatively correlated to phosphate and chlorophyll-a concentration (n.s.). In addition, phosphate, nitrate, sulfate and iron concentrations were elevated in streams that experience significant storm-water runoff and these streams also tended to have lower macroinvertebrate abundance, diversity and richness. These elevated nutrients, phosphorus in particular, were rapidly taken up by the benthic algae as evidenced by declining nutrients, and increased algal pigment and organic matter concentration from up to down-stream (n.s.). Biological uptake did not translate into increased macroinvertebrate abundance, likely because of the flashy discharge regime. Combination of non EPT (Ephemeroptera, Plecoptera, and Trichoptera) metrics indicated that the Shire and Junkyard ravines were in better condition than sites at Isengard and Fangorn—patterns which strongly suggest that extent of storm-water runoff has negatively impacted the macroinvertebrate communities. The fish community assessment indicated that blacknose dace (Rhinichthys atratulus), a species known to prefer cold, clean water, was most abundant in the Shire—the most pristine ravine stream sampled. Comparing length/weight data in the sampled streams to state standards indicated that these dace are not as fit as typically found in other water bodies whereas other taxa, namely the white sucker (Catostomus commersonii) and creek chub (Somotillus atromaculatus) are indistinguishable from state fitness standards. We were successful establishing biological base-line conditions prior to the initiation of a campus wide storm-water abatement program and can use these benchmarks to gage the long-term efficacy of restoration using physicochemical, population, community and ecosystem functional attributes measured in these unique ecosystems

Automated analysis of 16-color polychromatic flow cytometry data maps immune cell populations and reveals a distinct inhibitory receptor signature in systemic sclerosis

Background. The phenotypic profiles of both peripheral blood and tissue-resident immune cells have been linked to the health status of individuals with infectious and autoimmune diseases, as well as cancer. In light of the promising clinical trial results of agents that block the Inhibitory Receptor (IR) Programmed Death 1 (PD-1) axis, novel flow cytometric panels that simultaneously measure multiple IRs on several immune cell subsets could provide the distinct IR signatures to target in combinational therapies for many disease states. Also, due to the paucity of human samples, larger (14+ color) ‘1-tube’ panels for immune cell characterization ex vivo are of a high value in translational studies. Development of fluorescent-based panels offer several advantages as compared with analogous mass cytometric methods, including the ability to sort multiple populations of interest from the sample for further study. However, automated platforms of multi-dimensional single cell analysis that allow objective and comprehensive population characterization are severely underutilized on data generated from large polychromatic panels. Methods. A 16-color flow cytometry (FCM) panel was developed and optimized for the simultaneous characterization and purification of multiple human immune cell populations on a 4- laser BD FACSARIA II cell sorter. FCM data of samples obtained from healthy subjects and individuals with systemic sclerosis (SSc) were loaded into Cytobank cloud, then compensated and analyzed with SPADE clustering algorithm. The viSNE algorithm was also employed to compress the data into a 2D map of phenotypic space that was subsequently clustered using SPADE. For comparison, the FCM data were also analyzed manually using FlowJo software. Results. Our novel 16-color panel recognizes CD3, CD4, CD8, CD45RO, CD25, CD127, CD16, CD56, γδTCR, vα24, PD-1, LAG-3, CTLA-4, and TIM-3; it also contains a CD1d-tetramer and a live-dead dye (with CD19 and CD14 included as a combined dump channel). This panel allows combinational IR signatures to be determined from CD4+ T, CD8+ T, Natural Killer (NK), invariant Natural Killer (iNKT), and gamma delta (γδ) immune cell subsets within one sample. We have successfully identified all subsets of interest using automatic SPADE and viSNE algorithms integrated into Cytobank services, and demonstrated a distinctive phenotype of IR distribution on healthy versus systemic sclerosis subject groups. Conclusions. Methods of automatic analysis that were originally developed for processing multi-dimensional mass cytometry can be applied to polychromatic FCM datasets and provide robust results, including subset identification and distinct IR signatures in healthy compared to diseased subject groups

Improved Peddler for the Ann Arbor Veterans Affairs Healthcare System

ME450 Capstone Design and Manufacturing Experience: Fall 2015The Ann Arbor Veterans Association (AAVA) has a Home Based Cardiovascular Rehabilitation (HBCR) program for patients who have experienced a significant cardiac event and live in areas far from the AAVA. A peddler sent home with veterans for this program has been identified as displeasing and uncomfortable to use; we have been tasked in redesigning the machine to fit the needs of healthcare professionals and alleviate the complaints expressed by users. The areas we have determined that need redesign are: application of pedaling resistance, pedal design, a time and distance display, and anchoring of the peddler during use - all while maintaining the ability to pedal with hands or feet and a relatively compact and lightweight peddler that will be portable for veterans of all ages. The concepts we have chosen to meet these needs are a band brake to apply resistance to a rotor, larger pedals, a bike computer, and increased coefficient of friction between the peddler and the surface. Our final design incorporates these chosen concepts into a complete peddler which meets the key specifications.http://deepblue.lib.umich.edu/bitstream/2027.42/117342/1/ME450-F15-Project25-FinalReport.pd

Post, Share, Like: The Role of Facebook in the Russo-Ukrainian War

Facebook is being used by both Russia and Ukraine as a tool of war, for very different purposes. This demonstrates that the platform no longer serves the sole function of connecting communities together. Existing literature has recognized that social media is being used in the current Russo-Ukrainian war but has yet to conduct comparative and contrastive analyses of Russian and Ukrainian social media strategies and effects. Conducting these analyses will illustrate not only what strategies are being used, but how they can be simultaneously advantageous and disadvantageous for belligerents. By focusing on one platform, Facebook, one can not only learn why it is of crucial importance to both countries, but how the platform might be used moving forward. The findings of this paper suggest that Russian and Ukrainian tactics on Facebook are similar in at least six ways, but on the whole, they differ more than they coincide. The six coinciding tactics include funding, documentation on the ground, narrative spreading, heightening morale, name-calling, and utilization of the platform by leaders. Additionally, the effect of any given strategy varies, with some being successful, and others unsuccessful. Ultimately, these findings can serve as a resource for the national security, social media, political, legal, and academic communities

Improving medication safety in varied health systems

Non peer reviewe

Heterogeneous Spine Loss in Layer 5 Cortical Neurons after Spinal Cord Injury

A large thoracic spinal cord injury disconnects the hindlimb (HL) sensory-motor cortex from its target, the lumbar spinal cord. The fate of the synaptic structures of the axotomized cortical neurons is not well studied. We evaluated the density of spines on axotomized corticospinal neurons at 3, 7, and 21 days after the injury in adult mice expressing yellow fluorescence protein in a subset of layer 5 neurons. Spine density of the dendritic segment proximal to the soma (in layer 5) declined as early as 3 days after injury, far preceding the onset of somatic atrophy. In the distal segment (in layer 2/3), spine loss was slower and less severe than in the proximal segment. Axotomy of corticospinal axons in the brainstem (pyramidotomy) induced a comparable reduction of spine density, demonstrating that the loss is not restricted to the neurons axotomized in the thoracic spinal cord. Surprisingly, in both forms of injury, the spine density of putative non-axotomized layer 5 neurons was reduced as well. The spine loss may reflect fast rearrangements of cortical circuits after axotomy, for example, by a disconnection of HL cortical neurons from synaptic inputs that no longer provide useful informatio

Meet Your Regulators

In this session, representatives from Indiana Department of Environmental Management, Indiana Department of Natural Resources, US Army Corps of Engineers, and US Fish and Wildlife Service will discuss their roles in transportation projects as well as best practices and areas of concern they see when reviewing projects. Agency roles include waterway permitting, endangered species, historic preservation, and biological/floodway concerns

Lessons from the Health Care Innovation Awards: Productively Engaging Vulnerable Populations to Address Social Determinants of Health

Objectives: This paper examines productive interactions—a cornerstone of the Chronic Care Model—between health care providers and vulnerable populations with chronic illnesses and/or disabilities. Methods: We conducted qualitative analysis of 16 focus groups and 29 interviews with patients and/or caregivers and 195 interviews with program leadership and providers across 15 Health Care Innovation Awards that targeted vulnerable populations. We analyzed how awardees addressed health concerns and social determinants of health (SDOH), and identified key components of productive interactions. Results: Providers achieved productive interactions through four primary strategies: establishing trust and showing respect; solving problems; building accuracy in health information exchange; and sharing accountability and responsibility. While providers sought cooperation from patients and caregivers for medical goals, they often addressed SDOH priorities. Discussion: Strategies tailored to vulnerable populations can enable shared decision-making and effective self-care. A nonjudgmental engagement style, accurate information, and consistent communication are important for patient engagement

Community Research Collaboratives

This policy memo discusses an approach to research and engagement called Community Research Collaboratives (CRCs). Its goal is to further define and elevate an existing field of work currently under-recognized by academic researchers, philanthropists, and educational policymakers and practitioners. The authors describe CRCs as partnerships among community members, researchers, and/or educational institutions that work together to advance equity and justice by jointly creating new knowledge, policies, and/or practices that promote systemic change in educational systems and society. The authors' analysis is intended to highlight the unique attributes of CRCs, to boost their visibility in education, and to encourage funding support for this growing field.</p