Evidence for orbital forcing of Middle Cambrian peritidal cycles: Wah Wah range, south-central Utah

We have applied a new method (gamma method) for constructing high-resolution age models to peritidal cycles in the Middle Cambrian Pierson Cove Formation (13 cycles) and the Trippe Limestone (40 cycles) exposed in the Wah Wah range, south-central Utah. Spectral analyses of the time series for the gamma age model indicate the presence of significant spectral peaks (relative to a null model) in both data sets. After experimenting with different assumptions for the duration of the mean primary or measured cycle, we found that for the Trippe data set assigning the mean duration of precession to the mean primary cycle produced a reasonably good correlation between the spectrum and the early Paleozoic estimate of insolation forcing. In particular, the periods of the three significant spectral peaks in the Trippe record correspond to estimated line periods for eccentricity and precession and a combination tone of precession. A spectrum for the Trippe cycles based on the conventional assumption that time is proportional to thickness contained only one significant peak, and reasonable estimates of the duration of the mean primary cycle produced a poor fit to the insolation model. Spectral results from the Pierson Cove cycles were less compelling, possibly because of the short length of the record. The presence in the Trippe spectrum of significant peaks with periods corresponding to high-frequency orbital variations suggests that preservation of high-frequency Milankovitch signals is more common than implied by models of shallow marine cyclicity based on Pleistocene sea-level records. The results of these spectral analyses suggest that the gamma method can be used to construct age models for peritidal carbonate cycles that are accurate enough to test for periodicity and deterministic mechanisms, even in rocks as old as the Cambrian

Evidence for orbital forcing of Middle Cambrian peritidal cycles: Wah Wah range, south-central Utah

We have applied a new method (gamma method) for constructing high-resolution age models to peritidal cycles in the Middle Cambrian Pierson Cove Formation (13 cycles) and the Trippe Limestone (40 cycles) exposed in the Wah Wah range, south-central Utah. Spectral analyses of the time series for the gamma age model indicate the presence of significant spectral peaks (relative to a null model) in both data sets. After experimenting with different assumptions for the duration of the mean primary or measured cycle, we found that for the Trippe data set assigning the mean duration of precession to the mean primary cycle produced a reasonably good correlation between the spectrum and the early Paleozoic estimate of insolation forcing. In particular, the periods of the three significant spectral peaks in the Trippe record correspond to estimated line periods for eccentricity and precession and a combination tone of precession. A spectrum for the Trippe cycles based on the conventional assumption that time is proportional to thickness contained only one significant peak, and reasonable estimates of the duration of the mean primary cycle produced a poor fit to the insolation model. Spectral results from the Pierson Cove cycles were less compelling, possibly because of the short length of the record. The presence in the Trippe spectrum of significant peaks with periods corresponding to high-frequency orbital variations suggests that preservation of high-frequency Milankovitch signals is more common than implied by models of shallow marine cyclicity based on Pleistocene sea-level records. The results of these spectral analyses suggest that the gamma method can be used to construct age models for peritidal carbonate cycles that are accurate enough to test for periodicity and deterministic mechanisms, even in rocks as old as the Cambrian

Are cyclic sediments periodic? Gamma analysis and spectral analysis of Newark Supergroup lacustrine strata

Methodologies are suggested for the analysis of cyclic sediments. These include (1) linear analysis to determine whether cycles are of approximately constant duration and whether the relation between thickness and time is facies dependent and (2) multiple prolate-spheroidal windowing spectral analysis to determine whether time-series data indicate periodicities, either of the primary cycles or of higher or lower orders. The results of both methods are compared to a null hypothesis as a semiquantitative test of periodicity. Application of the methods to Newark Supergroup lacustrine cycles suggests that the primary cycles are approximately periodic and record a response to astronomical precession. The time represented by a given thickness of the different facies increases with the depositional water depth of that facies and with decreasing grain size. Precessional index cycles and long-period precessional index beats, or eccentricity, are strongly recorded in the spectra. Spectral results suggest but do not prove lengthening of the periodicities of orbital parameters since 200 Ma

Are cyclic sediments periodic? Gamma analysis and spectral analysis of Newark Supergroup lacustrine strata

Methodologies are suggested for the analysis of cyclic sediments. These include (1) linear analysis to determine whether cycles are of approximately constant duration and whether the relation between thickness and time is facies dependent and (2) multiple prolate-spheroidal windowing spectral analysis to determine whether time-series data indicate periodicities, either of the primary cycles or of higher or lower orders. The results of both methods are compared to a null hypothesis as a semiquantitative test of periodicity. Application of the methods to Newark Supergroup lacustrine cycles suggests that the primary cycles are approximately periodic and record a response to astronomical precession. The time represented by a given thickness of the different facies increases with the depositional water depth of that facies and with decreasing grain size. Precessional index cycles and long-period precessional index beats, or eccentricity, are strongly recorded in the spectra. Spectral results suggest but do not prove lengthening of the periodicities of orbital parameters since 200 Ma

Endoplasmic reticulum and lysosomal Ca2+ stores are remodelled in GBA1-linked Parkinson disease patient fibroblasts.

Mutations in β-glucocerebrosidase (encoded by GBA1) cause Gaucher disease (GD), a lysosomal storage disorder, and increase the risk of developing Parkinson disease (PD). The pathogenetic relationship between the two disorders is unclear. Here, we characterised Ca2+ release in fibroblasts from type I GD and PD patients together with age-matched, asymptomatic carriers, all with the common N370S mutation in β-glucocerebrosidase. We show that endoplasmic reticulum (ER) Ca2+ release was potentiated in GD and PD patient fibroblasts but not in cells from asymptomatic carriers. ER Ca2+ signalling was also potentiated in fibroblasts from aged healthy subjects relative to younger individuals but not further increased in aged PD patient cells. Chemical or molecular inhibition of β-glucocerebrosidase in fibroblasts and a neuronal cell line did not affect ER Ca2+ signalling suggesting defects are independent of enzymatic activity loss. Conversely, lysosomal Ca2+ store content was reduced in PD fibroblasts and associated with age-dependent alterations in lysosomal morphology. Accelerated remodelling of Ca2+ stores by pathogenic GBA1 mutations may therefore feature in PD

Visual short-term memory deficits associated with GBA mutation and Parkinson's disease.

Individuals with mutation in the lysosomal enzyme glucocerebrosidase (GBA) gene are at significantly high risk of developing Parkinson's disease with cognitive deficit. We examined whether visual short-term memory impairments, long associated with patients with Parkinson's disease, are also present in GBA-positive individuals-both with and without Parkinson's disease. Precision of visual working memory was measured using a serial order task in which participants observed four bars, each of a different colour and orientation, presented sequentially at screen centre. Afterwards, they were asked to adjust a coloured probe bar's orientation to match the orientation of the bar of the same colour in the sequence. An additional attentional 'filtering' condition tested patients' ability to selectively encode one of the four bars while ignoring the others. A sensorimotor task using the same stimuli controlled for perceptual and motor factors. There was a significant deficit in memory precision in GBA-positive individuals-with or without Parkinson's disease-as well as GBA-negative patients with Parkinson's disease, compared to healthy controls. Worst recall was observed in GBA-positive cases with Parkinson's disease. Although all groups were impaired in visual short-term memory, there was a double dissociation between sources of error associated with GBA mutation and Parkinson's disease. The deficit observed in GBA-positive individuals, regardless of whether they had Parkinson's disease, was explained by a systematic increase in interference from features of other items in memory: misbinding errors. In contrast, impairments in patients with Parkinson's disease, regardless of GBA status, was explained by increased random responses. Individuals who were GBA-positive and also had Parkinson's disease suffered from both types of error, demonstrating the worst performance. These findings provide evidence for dissociable signature deficits within the domain of visual short-term memory associated with GBA mutation and with Parkinson's disease. Identification of the specific pattern of cognitive impairment in GBA mutation versus Parkinson's disease is potentially important as it might help to identify individuals at risk of developing Parkinson's disease

An investigation of secondary students' engagement in a science inquiry through a student–scientist partnership

In 2011, Year 9 science extension students at a regional Australian secondary school participated in a 'full scientific inquiry' (US National Academy of Sciences, 2000), wherein they undertook plant growth trials to investigate the capacity of biochar, a fine-grained charcoal, to enhance the nutrient quality of compost. The inquiry was planned and guided by their teacher and a scientist, who aimed to afford high-performing, junior secondary students an opportunity to work alongside a 'real scientist' and experience the 'thinking and doing of science' (Hume & Coll, 2010). Qualitative data emanating from semi-structured interviews with nine students, as well as the teacher and scientist, revealed high levels of student engagement in the inquiry focus, given potential benefits of biochar as a combined system for soil improvement and carbon sequestration. Students reported working with greater accuracy and purposefulness as inquiry results were not predetermined and were to inform pilot research for the scientist's doctorate. Quantitative data analysis revealed that students who participated in the biochar inquiry outperformed their science extension-level peers who did not participate in the inquiry, in both a state-wide science test and overall science grades. Findings of this study highlight positive outcomes to have resulted from the student–scientist partnership. At a time when the school science curriculum is failing to engage the majority of young people (Lyons, 2006), there is need for further research to evaluate the effectiveness of this strategy in enhancing student engagement and achievement in science in the compulsory years of schooling

A Human Neural Crest Stem Cell-Derived Dopaminergic Neuronal Model Recapitulates Biochemical Abnormalities in GBA1 Mutation Carriers

Numerically the most important risk factor for the development of Parkinson's disease (PD) is the presence of mutations in the glucocerebrosidase GBA1 gene. In vitro and in vivo studies show that GBA1 mutations reduce glucocerebrosidase (GCase) activity and are associated with increased α-synuclein levels, reflecting similar changes seen in idiopathic PD brain. We have developed a neural crest stem cell-derived dopaminergic neuronal model that recapitulates biochemical abnormalities in GBA1 mutation-associated PD. Cells showed reduced GCase protein and activity, impaired macroautophagy, and increased α-synuclein levels. Advantages of this approach include easy access to stem cells, no requirement to reprogram, and retention of the intact host genome. Treatment with a GCase chaperone increased GCase protein levels and activity, rescued the autophagic defects, and decreased α-synuclein levels. These results provide the basis for further investigation of GCase chaperones or similar drugs to slow the progression of PD