896 research outputs found
Bogomol'nyi Decomposition for Vesicles of Arbitrary Genus
We apply the Bogomol'nyi technique, which is usually invoked in the study of
solitons or models with topological invariants, to the case of elastic energy
of vesicles. We show that spontaneous bending contribution caused by any
deformation from metastable bending shapes falls in two distinct topological
sets: shapes of spherical topology and shapes of non-spherical topology
experience respectively a deviatoric bending contribution a la Fischer and a
mean curvature bending contribution a la Helfrich. In other words, topology may
be considered to describe bending phenomena. Besides, we calculate the bending
energy per genus and the bending closure energy regardless of the shape of the
vesicle. As an illustration we briefly consider geometrical frustration
phenomena experienced by magnetically coated vesicles.Comment: 8 pages, 1 figure; LaTeX2e + IOPar
Self-Dual Bending Theory for Vesicles
We present a self-dual bending theory that may enable a better understanding
of highly nonlinear global behavior observed in biological vesicles. Adopting
this topological approach for spherical vesicles of revolution allows us to
describe them as frustrated sine-Gordon kinks. Finally, to illustrate an
application of our results, we consider a spherical vesicle globally distorted
by two polar latex beads.Comment: 10 pages, 3 figures, LaTeX2e+IOPar
Phase ordering and shape deformation of two-phase membranes
Within a coupled-field Ginzburg-Landau model we study analytically phase
separation and accompanying shape deformation on a two-phase elastic membrane
in simple geometries such as cylinders, spheres and tori. Using an exact
periodic domain wall solution we solve for the shape and phase ordering field,
and estimate the degree of deformation of the membrane. The results are
pertinent to a preferential phase separation in regions of differing curvature
on a variety of vesicles.Comment: 4 pages, submitted to PR
Transcritical flow of a stratified fluid: The forced extended Korteweg-de Vries model
Transcritical, or resonant, flow of a stratified fluid over an obstacle is studied using a forced extended Korteweg-de Vries model. This model is particularly relevant for a two-layer fluid when the layer depths are near critical, but can also be useful in other similar circumstances. Both quadratic and cubic nonlinearities are present and they are balanced by third-order dispersion. We consider both possible signs for the cubic nonlinear term but emphasize the less-studied case when the cubic nonlinear term and the dispersion term have the same-signed coefficients. In this case, our numerical computations show that two kinds of solitary waves are found in certain parameter regimes. One kind is similar to those of the well-known forced Korteweg-de Vries model and occurs when the cubic nonlinear term is rather small, while the other kind is irregularly generated waves of variable amplitude, which may continually interact. To explain this phenomenon, we develop a hydraulic theory in which the dispersion term in the model is omitted. This theory can predict the occurence of upstream and downstream undular bores, and these predictions are found to agree quite well with the numerical computations. © 2002 American Institute of Physics.published_or_final_versio
Decreased MCM2-6 in Drosophila S2 cells does not generate significant DNA damage or cause a marked increase in sensitivity to replication interference.
A reduction in the level of some MCM proteins in human cancer cells (MCM5 in U20S cells or MCM3 in Hela cells) causes a rapid increase in the level of DNA damage under normal conditions of cell proliferation and a loss of viability when the cells are subjected to replication interference. Here we show that Drosophila S2 cells do not appear to show the same degree of sensitivity to MCM2-6 reduction. Under normal cell growth conditions a reduction of >95% in the levels of MCM3, 5, and 6 causes no significant short term alteration in the parameters of DNA replication or increase in DNA damage. MCM depleted cells challenged with HU do show a decrease in the density of replication forks compared to cells with normal levels of MCM proteins, but this produces no consistent change in the levels of DNA damage observed. In contrast a comparable reduction of MCM7 levels has marked effects on viability, replication parameters and DNA damage in the absence of HU treatment
Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy
Development of single-molecule localization microscopy techniques has allowed nanometre scale localization accuracy inside cells, permitting the resolution of ultra-fine cell structure and the elucidation of crucial molecular mechanisms. Application of these methodologies to understanding processes underlying DNA replication and repair has been limited to defined in vitro biochemical analysis and prokaryotic cells. In order to expand these techniques to eukaryotic systems, we have further developed a photo-activated localization microscopy-based method to directly visualize DNA-associated proteins in unfixed eukaryotic cells. We demonstrate that motion blurring of fluorescence due to protein diffusivity can be used to selectively image the DNA-bound population of proteins. We designed and tested a simple methodology and show that it can be used to detect changes in DNA binding of a replicative helicase subunit, Mcm4, and the replication sliding clamp, PCNA, between different stages of the cell cycle and between distinct genetic backgrounds
Photoluminescent diamond nanoparticles for cell labeling: study of the uptake mechanism in mammalian cells
Diamond nanoparticles (nanodiamonds) have been recently proposed as new
labels for cellular imaging. For small nanodiamonds (size <40 nm) resonant
laser scattering and Raman scattering cross-sections are too small to allow
single nanoparticle observation. Nanodiamonds can however be rendered
photoluminescent with a perfect photostability at room temperature. Such a
remarkable property allows easier single-particle tracking over long
time-scales. In this work we use photoluminescent nanodiamonds of size <50 nm
for intracellular labeling and investigate the mechanism of their uptake by
living cells . By blocking selectively different uptake processes we show that
nanodiamonds enter cells mainly by endocytosis and converging data indicate
that it is clathrin mediated. We also examine nanodiamonds intracellular
localization in endocytic vesicles using immunofluorescence and transmission
electron microscopy. We find a high degree of colocalization between vesicles
and the biggest nanoparticles or aggregates, while the smallest particles
appear free in the cytosol. Our results pave the way for the use of
photoluminescent nanodiamonds in targeted intracellular labeling or biomolecule
deliver
Synthesis of Fluorine-18 Functionalized Nanoparticles for use as in vivo Molecular Imaging Agents
Nanoparticles containing fluorine-18 were prepared from block copolymers made by ring opening metathesis polymerization (ROMP). Using the fast initiating ruthenium metathesis catalyst (H_2IMes)(pyr)_2(Cl)_2Ru=CHPh, low polydispersity amphiphilic block copolymers were prepared from a cinnamoyl-containing hydrophobic norbornene monomer and a mesyl-terminated PEG-containing hydrophilic norbornene monomer. Self-assembly into micelles and subsequent cross-linking of the micelle cores by light-activated dimerization of the cinnamoyl groups yielded stable nanoparticles. Incorporation of fluorine-18 was achieved by nucleophilic displacement of the mesylates by the radioactive fluoride ion with 31% incorporation of radioactivity. The resulting positron-emitting nanoparticles are to be used as in vivo molecular imaging agents for use in tumor imaging
Multiband tight-binding theory of disordered ABC semiconductor quantum dots: Application to the optical properties of alloyed CdZnSe nanocrystals
Zero-dimensional nanocrystals, as obtained by chemical synthesis, offer a
broad range of applications, as their spectrum and thus their excitation gap
can be tailored by variation of their size. Additionally, nanocrystals of the
type ABC can be realized by alloying of two pure compound semiconductor
materials AC and BC, which allows for a continuous tuning of their absorption
and emission spectrum with the concentration x. We use the single-particle
energies and wave functions calculated from a multiband sp^3 empirical
tight-binding model in combination with the configuration interaction scheme to
calculate the optical properties of CdZnSe nanocrystals with a spherical shape.
In contrast to common mean-field approaches like the virtual crystal
approximation (VCA), we treat the disorder on a microscopic level by taking
into account a finite number of realizations for each size and concentration.
We then compare the results for the optical properties with recent experimental
data and calculate the optical bowing coefficient for further sizes
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