GRAND CHALLENGE No. 4: CURRICULUM DESIGN – Curriculum Matters: Case Studies from Canada and the UK

Archaeology in the 21st century faces outward more than inward, with many archaeologists working on projects that actively involve young people, descendant communities, diverse colleagues and clients, and the general public. The ways and means of learning and teaching about the past, as outlined in the curricula of primary, secondary, and post-secondary schools, always reflect the prevalent pedagogies of the age. Our paper comments upon two different ways of learning about archaeology. First, it presents an online university graduate program in Canada for post-Baccalaureate Cultural Resource Management (CRM) practitioners and a module on archaeology and education, which may form part of a variety of Master’s degrees in the UK. Second, it examines the ways in which archaeology has been introduced into a range of subjects in the National Curricula of the UK. Our goal is to inspire critical reflection upon the connections between the social milieu in which we teach and learn and the scope and focus of curricula and pedagogy in archaeology. We conclude with comments on current dynamics and desired futures at the fascinating interface of archaeology and education

Applications of advanced diffractive optical elements

Digital Optics Corporation is a UNC-Charlotte spin-off company, established to transfer technology developed at UNC-Charlotte for the design and manufacture Computer Generated Holograms (CGH's) and to market products based on CGH technology. DOC acquired core technologies from UNC-Charlotte including: (1) a CGH encoding process that can provide holograms with extremely high diffraction efficiency; (2) a low cost, high precision CGH manufacturing process; and (3) extensive holographic and refractive element design capabilities for design and evaluation of complex optical systems. These technologies have been used to design and/or manufacture optical components for a variety of applications including: (1) generation of Spot arrays; (2) fiber optic coupling elements; (3) optical interconnects between VLSI chips within and between multichip modules; and (4) imaging systems for head-mounted displays (HMD's)

Als3 is a Candida albicans invasin that binds to cadherins and induces endocytosis by host cells.

Candida albicans is the most common cause of hematogenously disseminated and oropharyngeal candidiasis. Both of these diseases are characterized by fungal invasion of host cells. Previously, we have found that C. albicans hyphae invade endothelial cells and oral epithelial cells in vitro by inducing their own endocytosis. Therefore, we set out to identify the fungal surface protein and host cell receptors that mediate this process. We found that the C. albicans Als3 is required for the organism to be endocytosed by human umbilical vein endothelial cells and two different human oral epithelial lines. Affinity purification experiments with wild-type and an als3delta/als3delta mutant strain of C. albicans demonstrated that Als3 was required for C. albicans to bind to multiple host cell surface proteins, including N-cadherin on endothelial cells and E-cadherin on oral epithelial cells. Furthermore, latex beads coated with the recombinant N-terminal portion of Als3 were endocytosed by Chinese hamster ovary cells expressing human N-cadherin or E-cadherin, whereas control beads coated with bovine serum albumin were not. Molecular modeling of the interactions of the N-terminal region of Als3 with the ectodomains of N-cadherin and E-cadherin indicated that the binding parameters of Als3 to either cadherin are similar to those of cadherin-cadherin binding. Therefore, Als3 is a fungal invasin that mimics host cell cadherins and induces endocytosis by binding to N-cadherin on endothelial cells and E-cadherin on oral epithelial cells. These results uncover the first known fungal invasin and provide evidence that C. albicans Als3 is a molecular mimic of human cadherins

Influence of social networks on the adoption of climate smart technologies in East Africa: Findings from two surveys and participatory exercises with farmers and local experts

As part of the Policy Action for Climate Change Adaptation (PACCA) project, this info note summarizes findings of a project activity entitled “Influencing and linking policies and institutions from national to local level for the development and adoption of climate‐resilient food systems in East Africa” undertaken by researchers from Bioversity International and Arizona State University. By conducting a network analysis and participatory exercises with district officials and farmers in Lushoto (Tanzania) and Rakai (Uganda), the study assesses the extent to which farmers are adopting agricultural practices and correlates the findings about the size and “make up” of the networks in which the farmers are embedded

Adoption of climate smart technologies in East Africa: Findings from two surveys and participatory exercises with farmers and local experts

As part of the “Policy Action for Climate Change Adaptation” (PACCA) project this info note summarizes findings of a project activity entitled “Influencing and linking policies and institutions from national to local level for the development and adoption of climate‐resilient food systems in East Africa” undertaken by researchers from Bioversity International and Arizona State University. By conducting a network analysis and participatory exercises with district officials and farmers, the study assesses the extent to which farmers are adopting agricultural practices and correlates the findings about the size and “make up” of the networks in which the farmers are embedded

Structural correlates of antimicrobial efficacy in IL-8 and related human kinocidins

AbstractChemokines are small (8–12 kDa) effector proteins that potentiate leukocyte chemonavigation. Beyond this role, certain chemokines have direct antimicrobial activity against human pathogenic organisms; such molecules are termed kinocidins. The current investigation was designed to explore the structure–activity basis for direct microbicidal activity of kinocidins. Amino acid sequence and 3-dimensional analyses demonstrated these molecules to contain iterations of the conserved γ-core motif found in broad classes of classical antimicrobial peptides. Representative CXC, CC and C cysteine-motif-group kinocidins were tested for antimicrobial activity versus human pathogenic bacteria and fungi. Results demonstrate that these molecules exert direct antimicrobial activity in vitro, including antibacterial activity of native IL-8 and MCP-1, and microbicidal activity of native IL-8. To define molecular determinants governing its antimicrobial activities, the IL-8 γ-core (IL-8γ) and α-helical (IL-8α) motifs were compared to native IL-8 for antimicrobial efficacy in vitro. Microbicidal activity recapitulating that of native IL-8 localized to the autonomous IL-8α motif in vitro, and demonstrated durable microbicidal activity in human blood and blood matrices ex vivo. These results offer new insights into the modular architecture, context-related deployment and function, and evolution of host defense molecules containing γ-core motifs and microbicidal helices associated with antimicrobial activity

A multimodal spectroscopy system for real-time disease diagnosis

The combination of reflectance, fluorescence, and Raman spectroscopy—termed multimodal spectroscopy (MMS)—provides complementary and depth-sensitive information about tissue composition. As such, MMS is a promising tool for disease diagnosis, particularly in atherosclerosis and breast cancer. We have developed an integrated MMS instrument and optical fiber spectral probe for simultaneous collection of all three modalities in a clinical setting. The MMS instrument multiplexes three excitation sources, a xenon flash lamp (370–740 nm), a nitrogen laser (337 nm), and a diode laser (830 nm), through the MMS probe to excite tissue and collect the spectra. The spectra are recorded on two spectrograph/charge-coupled device modules, one optimized for visible wavelengths (reflectance and fluorescence) and the other for the near-infrared (Raman), and processed to provide diagnostic parameters. We also describe the design and calibration of a unitary MMS optical fiber probe 2 mm in outer diameter, containing a single appropriately filtered excitation fiber and a ring of 15 collection fibers, with separate groups of appropriately filtered fibers for efficiently collecting reflectance, fluorescence, and Raman spectra from the same tissue location. A probe with this excitation/collection geometry has not been used previously to collect reflectance and fluorescence spectra, and thus physical tissue models (“phantoms”) are used to characterize the probe’s spectroscopic response. This calibration provides probe-specific modeling parameters that enable accurate extraction of spectral parameters. This clinical MMS system has been used recently to analyze artery and breast tissue in vivo and ex vivo.National Institutes of Health (U.S) ( Grant No. P41-RR-02594

Differential hRad17 expression by histologic subtype of ovarian cancer

<p>Abstract</p> <p>Background</p> <p>In the search for unique ovarian cancer biomarkers, ovarian specific cDNA microarray analysis identified hRad17, a cell cycle checkpoint protein, as over-expressed in ovarian cancer. The aim of this study was to validate this expression.</p> <p>Methods</p> <p>Immunohistochemistry was performed on 72 serous, 19 endometrioid, 10 clear cell, and 6 mucinous ovarian cancers, 9 benign ovarian tumors, and 6 normal ovarian tissue sections using an anti-hRad17 antibody. Western blot analysis and quantitative PCR were performed using cell lysates and total RNA prepared from 17 ovarian cancer cell lines and 6 normal ovarian epithelial cell cultures (HOSE).</p> <p>Results</p> <p>Antibody staining confirmed upregulation of hRad17 in 49.5% of ovarian cancer cases. Immunohistochemistry demonstrated that only 42% of serous and 47% of endometrioid subtypes showed overexpression compared to 80% of clear cell and 100% of mucinous cancers. Western blot confirmed overexpression of hRad17 in cancer cell lines compared to HOSE. Quantitative PCR demonstrated an upregulation of hRad17 RNA by 1.5-7 fold. hRad17 RNA expression differed by subtype.</p> <p>Conclusions</p> <p>hRad17 is over-expressed in ovarian cancer. This over-expression varies by subtype suggesting a role in the pathogenesis of these types. Functional studies are needed to determine the potential role of this protein in ovarian cancer.</p

Reverse Engineering of Computer-Based Navy Systems

The financial pressure to meet the need for change in computer-based systems through evolution rather than through revolution has spawned the discipline of reengineering. One driving factor of reengineering is that it is increasingly becoming the case that enhanced requirements placed on computer-based systems are overstressing the processing resources of the systems. Thus, the distribution of processing load over highly parallel and distributed hardware architectures has become part of the reengineering process for computer-based Navy systems. This paper presents an intermediate representation (IR) for capturing features of computer-based systems to enable reengineering for concurrency. A novel feature of the IR is that it incorporates the mission critical software architecture, a view that enables information to be captured at five levels of granularity: the element/program level, the task level, the module/class/package level, the method/procedure level, and the statement/instruction level. An approach to reverse engineering is presented, in which the IR is captured, and is analyzed to identify potential concurrency. Thus, the paper defines concurrency metrics to guide the reengineering tasks of identifying, enhancing, and assessing concurrency, and for performing partitioning and assignment. Concurrency metrics are defined at several tiers of the mission critical software architecture. In addition to contributing an approach to reverse engineering for computer-based systems, the paper also discusses a reverse engineering analysis toolset that constructs and displays the IR and the concurrency metrics for Ada programs. Additionally, the paper contains a discussion of the context of our reengineering efforts within the United States Navy, by describing two reengineering projects focused on sussystems of the AEGIS Weapon System