FARM MACHINERY INVESTMENT AND THE TAX REFORM ACT OF 1986

The Tax Reform Act of 1986 significantly changed incentives for investing. This analysis specifically examines how changes in marginal tax rates, depreciation schedules, and the investment tax credit altered the cost of capital and net investment in agriculture. A stochastic coefficients econometric methodology is used to estimate an investment function which is then used to simulate the effects of tax reform. Estimates indicated that relative to prior law, the Tax Reform Act will reduce the capital stock of farm machinery and equipment by nearly $4 billion.Agricultural Finance, Farm Management,

AGGREGATE FRUIT PRICE EQUATIONS AND CONDITIONAL PRICE FORECASTS

Demand and Price Analysis,

Paleoseismicity derived from piston coring methods, Explorer and Juan de Fuca Plate systems, Bristish Columbia

Coring of marine sediments has revealed deposits related to slope instability induced by seismicity on the western margin of Canada. Debris flows and turbidite sequences related to megathrust earthquakes have been recovered in six piston cores on the Juan de Fuca and Explorer tectonic plates, allowing comparison of the response of each plate to shaking during great earthquakes. Analyses of the recovered cores show that turbidite sequences associated with a megathrust quake occur on the Juan de Fuca Plate and do not occur in cores collected 90 km away at a similar site on the Explorer Plate. The record of subduction-related earthquake turbidite sequences is not complete at the Juan de Fuca study area and no reconstruction of megathrust quake periodicity is thus possible using this site alone. These results indicate that strong ground shaking is probably not experienced during large subduction earthquakes on the Explorer Plate

Inositol 1,4,5-trisphosphate supports the arrhythmogenic action of endothelin-1 on ventricular cardiac myocytes

Although ventricular cardiomyocytes express inositol 1,4,5-trisphosphate [Ins(1,4,5)Ρ3] receptors, it is unclear how these Ca2+ channels contribute to the effects of Gq-coupled agonists. Endothelin-1 augmented the amplitude of pacing-evoked Ca2+ signals (positive inotropy), and caused an increasing frequency of spontaneous diastolic Ca2+-release transients. Both effects of endothelin-1 were blocked by an antagonist of phospholipase C, suggesting that Ins(1,4,5)Ρ3 and/or diacylglycerol production was necessary. The endothelin-1-mediated spontaneous Ca2+ transients were abolished by application of 2-aminoethoxydiphenyl borate (2-APB), an antagonist of Ins(1,4,5)Ρ3 receptors. Incubation of electrically-paced ventricular myocytes with a membrane-permeant Ins(1,4,5)Ρ3 ester provoked the occurrence of spontaneous diastolic Ca2+ transients with the same characteristics and sensitivity to 2-APB as the events stimulated by endothelin-1. In addition to evoking spontaneous Ca2+ transients, stimulation of ventricular myocytes with the Ins(1,4,5)Ρ3 ester caused a positive inotropic effect. The effects of endothelin-1 were compared with two other stimuli, isoproterenol and digoxin, which are known to induce inotropy and spontaneous Ca2+ transients by overloading intracellular Ca2+ stores. The events evoked by isoproterenol and digoxin were dissimilar from those triggered by endothelin-1 in several ways. We propose that Ins(1,4,5)Ρ3 receptors support the development of both inotropy and spontaneous pro-arrhythmic Ca2+ signals in ventricular myocytes stimulated with a Gq-coupled agonist

Genetic and Tissue Engineering Approaches to Modeling the Mechanics of Human Heart Failure for Drug Discovery

Heart failure is the leading cause of death in the western world and as such, there is a great need for new therapies. Heart failure has a variable presentation in patients and a complex etiology; however, it is fundamentally a condition that affects the mechanics of cardiac contraction, preventing the heart from generating sufficient cardiac output under normal operating pressures. One of the major issues hindering the development of new therapies has been difficulties in developing appropriate in vitro model systems of human heart failure that recapitulate the essential changes in cardiac mechanics seen in the disease. Recent advances in stem cell technologies, genetic engineering, and tissue engineering have the potential to revolutionize our ability to model and study heart failure in vitro. Here, we review how these technologies are being applied to develop personalized models of heart failure and discover novel therapeutics

High power heating of magnetic reconnection in merging tokamak experimentsa)

Significant ion/electron heating of magnetic reconnection up to 1.2 keV was documented in two spherical tokamakplasma merging experiment on MAST with the significantly large Reynolds number R∼10⁵. Measured 1D/2D contours of ion and electron temperatures reveal clearly energy-conversion mechanisms of magnetic reconnection: huge outflow heating of ions in the downstream and localized heating of electrons at the X-point. Ions are accelerated up to the order of poloidal Alfven speed in the reconnection outflow region and are thermalized by fast shock-like density pileups formed in the downstreams, in agreement with recent solar satellite observations and PIC simulation results. The magnetic reconnection efficiently converts the reconnecting (poloidal) magnetic energy mostly into ion thermal energy through the outflow, causing the reconnectionheating energy proportional to square of the reconnecting (poloidal) magnetic field Brec²  ∼  Bp². The guide toroidal field Bt does not affect the bulk heating of ions and electrons, probably because the reconnection/outflow speeds are determined mostly by the external driven inflow by the help of another fast reconnection mechanism: intermittent sheet ejection. The localized electron heating at the X-point increases sharply with the guide toroidal field Bt, probably because the toroidal field increases electron confinement and acceleration length along the X-line. 2D measurements of magnetic field and temperatures in the TS-3 tokamak merging experiment also reveal the detailed reconnectionheating mechanisms mentioned above. The high-power heating of tokamak merging is useful not only for laboratory study of reconnection but also for economical startup and heating of tokamakplasmas. The MAST/TS-3 tokamak merging with Bp > 0.4 T will enables us to heat the plasma to the alpha heating regime: Ti > 5 keV without using any additional heating facility.This work was supported by a Grant-in-Aid for Scientific Research (A) No 22246119 and JSPS Core-to-Core program No 22001, the JSPS Institutional Program for Young Researcher Overseas Visits and NIFS Collaboration Research Programs (NIFS11KNWS001, NIFS12KLEH024, NIFS11KUTR060). This work was funded partly by the RCUK Energy Program under Grant No. EP/I501045 and the European Communities under the contract of CCFE

Colon carcinoma cells harboring PIK3CA mutations display resistance to growth factor deprivation induced apoptosis.

PIK3CA, encoding the p110alpha catalytic subunit of phosphatidylinositol 3-kinase (PI3K), is mutated in a variety of human cancers. We screened the colon cancer cell lines previously established in our laboratory for PIK3CA mutations and found that four of them harbored gain of function mutations. We have now compared a panel of mutant and wild-type cell lines for cell proliferation and survival in response to stress. There was little difference in PI3K activity between mutant PIK3CA-bearing cells (mutant cells) and wild-type PIK3CA-bearing cells (wild-type cells) under optimal growth conditions. However, the mutant cells showed constitutive PI3K activity during growth factor deprivation stress (GFDS), whereas PI3K activity decayed rapidly in the wild-type cells. Importantly, constitutively active PI3K rendered the mutant cells resistant to GFDS-induced apoptosis relative to the wild-type cells, indicating a biological advantage under stress conditions that is imparted by the mutant enzymes. Compared with the wild-type cells, the mutant cells were hypersensitive to the apoptosis induced by the PI3K inhibitor LY294002. In addition, PIK3CA small interfering RNA significantly decreased DNA synthesis and/or induced apoptosis in the mutant cells but not in the wild-type cells. Furthermore, ecotopic expression of a mutant PIK3CA in a nontumorigenic PIK3CA wild-type cell line resulted in resistance to GFDS-induced apoptosis, whereas transfection of wild-type PIK3CA or empty vector had little effect. Taken together, our studies show that mutant PIK3CA increases the capacity for proliferation and survival under environmental stresses, such as GFDS while also imparting greater dependency on the PI3K pathway for proliferation and survival

Overview of field operations during a 2013 research expedition to the southern Beaufort Sea on the RV Araon

Research experiments conducted and preliminary findings The Expedition ARA04C is a multidisciplinary research program in the Beaufort Sea, carried out in collaboration between the Korea Polar Research Institute (KOPRI), Geological Survey of Canada (GSC), Department of Fisheries and Ocean (DFO), Monterey Bay Aquarium Research Institute (MBARI), and the Alfred Wegener Institute (AWI). The Expedition ARA04C on the IBRV Araon took place from September 6 to September 24, 2013 (Figure 0.1). Multiple research experiments were undertaken to study geological processes related to degrading permafrost, fluid flow and degassing, and associated geohazards, paleo-oceanography of the Beaufort shelf and slope region, as well as physical and chemical oceanography measurement of the Arctic Ocean linked with continuous atmospheric studies. The expedition focused on two main research areas: offshore Barrow, Alaska, from September 7 to September 9, 2013, and the Canadian Beaufort Sea from September 10 to September 24, 2013. Multichannel seismic data, in conjunction with an ocean-bottom-seismometer (OBS) study were collected to support drilling proposals especially IODP pre-proposal #806 (Dallimore et al., 2012), and to verify distribution and internal structures of the offshore permafrost occurrences (Figure 0.2). The multi-channel seismic data were acquired on the outer continental shelf of the Canadian Beaufort Sea, totaling 14 lines with ~435 line-kilometers and ~4,500 shot gathers (Chapter 3). The combined multichannel seismic and OBS data will be processed post-expedition at KOPRI and the GSC, and will allow detailed velocity analyses to investigate the permafrost signature and help mapping zones of high-velocity sediments indicative of the presence of ice (Chapter 4). Individual shot gathers collected during the multichannel seismic program show clear refraction arrivals with velocities around 2000m/s in areas of expected permafrost occurrence, and shot gathers lacked such arrivals in zones where the permafrost was predicted to be absent. It is therefore expected that the OBS data, once processed, will also show clear refracted arrivals for velocity analyses. Continuous sub-bottom profiler (SBP) and multibeam data were collected along all ship tracks for detailed subsurface imaging of sediment structures and permafrost, as well as for core-site location verification (Chapter 5 and 6). During Expedition ARA04C, more than 3000 line-kilometers of SBP data were collected, co-located with multibeam and backscatter data. These data are an essential part of the study of sub-seafloor permafrost distribution and provide insights into sediment dynamics at critical boundaries, such as the shelf edge. Along the shelf edge, the occurrence of pingo-like features (PLFs) result in a rugged landscape with thousands of PLFs piercing through the otherwise laminated sediments. More than 30 crossings of this critical shelf-edge boundary were made during this expedition, which complement data acquired in 2012 with the Huntec system and 3.5 kHz data provided by ArcticNet as part of the regional multibeam map of the study area. High resolution data provided critical new insights in deep-water fluid expulsion zones. Key new data were acquired over the area of the "Gary Knolls", where PLF structures occur at the shelf edge in water depth of only 50 to 60 m. All SBP data from this expedition will be post-processed and analyzed for the presence of sub-seafloor permafrost, occurrence of the PLF structures and indications for fluid and gas migration. Multibeam and backscatter data were collected along all ship tracks, adding to the database of existing information gathered through previous expeditions to the study region. Heat flow measurements were undertaken at eight stations (Figure 0.3) to study the thermal structure of fluid expulsion features, as well as degrading permafrost along a slope-shelf transect in the eastern Mackenzie Trough (Chapter 7). The data provide critical constraints on the distribution of sub-seafloor permafrost as well as the gas hydrate stability zone around fluid expulsion features. A very important finding is the observation made at the mud volcano in 420 m water depth, where seafloor temperatures are the highest in all observed stations, indicating active mud volcanism. Geological sampling using gravity coring and multi-coring tools was performed at strategic sites to support two research objectives. The first objective was to provide key data towards ongoing international research linked to IODP pre-proposals #753 (O'Regan et al., 2010) and #806 (Dallimore et al., 2012). The second objective was to collect core to define key seismo-stratigraphic horizons critical to the understanding of geohazards in the region (Chapter 8). In total, 21 gravity cores and 12 multi-cores were taken (Figure 0.4, Table 8.3). All cores were scanned with a multi-sensor core-logger to measure physical properties (Chapter 9). Most sediment analyses on the cores will be performed post-expedition at KOPRI, GSC, and laboratories of other University-based collaborators in Canada and Germany. Onboard, sub-samples were taken from all shallow multi-cores and selected gravity cores. On selected cores from the Canadian Beaufort study region pore-waters were extracted using rhizones. These samples will be analyzed postexpedition at MBARI. Water sampling and Conductivity-Temperature-Depth (CTD) profiling was undertaken at most core sites to study physical and chemical properties of the seawater (Figure 0.5). These station-measurements were complemented by continuous waterproperty and atmospheric measurements when the Araon was underway. Most samples taken will be analyzed post-expedition at KOPRI for DIC/TA, nutrients, DOC, and POC. The pH of seawater, underway data of pCO2, CH4, and N2O, as well as a variety of subsequent calculations is required for accurate estimates in the above listed parameters. Methane was also measured with a methane sensor attached to the CTD tool and at the mud volcano in 420 m water depth, methane concentrations of more than 100-times ocean background were seen. The methane plume was also acoustically imaged with the echo sounder systems on board the IBRV Araon. Further details on the water sampling and atmospheric measurements are given in Chapter 10 and 11