Fabrication and Study of Power- Output of MultiChamber Microbial Fuel Cells (Mfcs) With Clay as Ion Exchange Partition

The main challenges in the construction of microbial fuel cells (MFCs) are the identification of materials, designs, and architectures that may maximize power generation efficiency and fabrication cost. In view of these facts, an attempt was made to design and fabricate Multi – Chamber MFCs of different configuration using locally available Mfensi clay as ionexchange partitions. The performance of each micro-cell, combined effect of the total system as one cell, and the overall performance were studied. The volume of each chamber of these cells was approximately 130 cm3 . It was found that the wastewater of chemical oxygen demand (COD) that was 6340 gm/L used in the MFCs yielded a maximum open circuit voltage (OCV) of 1421 ± 30 mV. The peak power density of 33.30 mW/cm2 (0.037 mA/cm2 ) at 1000 Ω was normalized to the anode surface are

Assessment of the Impact of Solid Waste Disposal on the Portability of Surface Water and Groundwater Using Water Quality Index (WQI) in Kpassa, Nkwanta North District of Ghana

The emergence of the Water Quality Index (WQI) has helped to convey vital water quality information to stakeholders and the general public at certain locations and times based on water quality parameters. The present study was carried out to assess how improper solid waste disposal impacts on the potability of surface and ground water at Kpassa in the Nkwanta North District of the Volta Region in Ghana. Water samples were collected at different locations on the River Kpassa and from boreholes within Kpassa in the Nkwanta North District of Ghana. The quality of the water was assessed by testing various physicochemical parameters such as pH, EC, Total Dissolved Solid (TDS), Total Hardness (TH), Calcium, Magnesium, Nitrate, Sulphate, Dissolved Oxygen (DO) and Biochemical Oxygen Demand (BOD). Apart from turbidity that was observed to be high in the samples during the period of study (indicating the presence of organic matter pollution, domestic effluents and runoffs from agricultural fields), all the other water quality parameters in both surface and groundwater were lower compared to WHO/ICMR/BIS standards.  The WQI for the surface water and the borehole water were 56.50 and 94.82 respectively. The study shows that the samples within the study area had poor water quality and therefore unsuitable for drinking and for domestic use. Regular water monitoring should be carried out especially with reference to the microbial analysis to give a better view of the water quality at Kpassa. Keywords: Kpassa, Index, pollution, agriculture fields, Leachat

Increasing the use of continuing professional development courses to strengthen trauma care in Ghana

Injury is a major cause of death and disability in Ghana. Strengthening care of the injured is essential to reduce this burden. Trauma continuing professional development (CPD) courses are an important component of strengthening trauma care. In many countries, including Ghana, their use needs to be more uniformly promoted. We propose lowcost strategies to increase the utilization of trauma CPD in Ghana, especially in district hospitals and higher need areas. These strategies include developing plans by regional health directorates and teaching hospitals for the regions for which they are responsible. Lists could be kept and monitored of which hospitals have doctors with which type of training. Those hospitals that need to have at least one doctor trained could be flagged for notice of upcoming courses in the area and especially encouraged to have the needed doctors attend. The targets should include at least one surgeon or one emergency physician at all regional or large district hospitals who have taken the Advanced Trauma Life Support (ATLS) (or locally-developed alternative) in the past 4 years, and each district hospital should have at least one doctor who has taken the Primary Trauma Care (PTC) or Trauma Evaluation and Management (TEAM) (or locally-developed alternatives) in the past 4 years. Parallel measures would increase enrollment in the courses during training, such as promoting TEAM for all medical students and ATLS for all surgery residents. It is important to develop and utilize more “home grown” alternatives to increase the long-term sustainability of these efforts, Keywords: trauma, injury, education, training, continuing professional developmentFunding: Non

IL-4 and IL-13 synergize to increase growth and CCL4 chemokine mRNA expression by 4T1 mammary adenocarcinomal tumor cells

Approximately, 1 in 8 women in the US will develop invasive breast cancer and 1 in 33 will die. Breast Cancer is a major health disparity for women all over the globe, having the largest impact on African American women under the age of 45. Despite medical advances, the 5-year survivorship among breast cancer patients remains significantly low largely due to high recurrence rates and propensity for metastasis to distal organs. (e.g., lung, liver, brain). Thus, further knowledge of the mechanisms which promote tumor development and progression is needed to advance cancer treatment. Tumor cells are known to secrete and express immune modulators as a mechanism to invade host antitumor immune responses. Using an experimental murine tumor cell line, our preliminary results demonstrate the expression of interleukin-4 receptor (IL-4R). We hypothesize that IL-4R activity plays a role in mediation of tumor cell function. In this study, 4T1 mammary adenocarcinoma cells were exposed to IL-4R ligands, interleukin-4 (IL-4), interleukin-13 (IL-13) and their ability to influence TNF-a, TGF-B1 and CCL4 expression by 4T1 cells. Our results demonstrated an increase growth and CCL4 mRNA expression in response to IL-4 in combination with IL-13, suggesting a potential target to mitigate tumor progression.Oklahoma Louis Stokes Alliance for Minority Participation ProgramNational Cancer Institute (U.S.)National Institue of Health (U.S.)National Heart, Lung, and Blood InstituteMicrobiology, Immunology and Genetic

A novel ruthenium-silver based antimicrobial potentiates aminoglycoside activity against Pseudomonas aeruginosa

The rapid dissemination of antibiotic resistance combined with the decline in the discovery of novel antibiotics represents a major challenge for infectious disease control that can only be mitigated by investments in novel treatment strategies. Alternative antimicrobials, including silver, have regained interest due to their diverse mechanisms of inhibiting microbial growth. One such example is AGXX, a broad-spec­trum antimicrobial that produces highly cytotoxic reactive oxygen species (ROS) to inflict extensive macromolecular damage. Due to the connections identified between ROS production and antibiotic lethality, we hypothesized that AGXX could potentially increase the activity of conventional antibiotics. Using the gram-negative pathogen Pseudomonas aeruginosa, we screened possible synergistic effects of AGXX on several antibiotic classes. We found that the combination of AGXX and aminoglycosides tested at sublethal concentrations led to a rapid exponential decrease in bacterial survival and restored the sensitivity of a kanamycin-resistant strain. ROS production contributes significantly to the bactericidal effects of AGXX/aminoglycoside treatments, which is dependent on oxygen availability and can be reduced by the addition of ROS scaveng­ers. Additionally, P. aeruginosa strains deficient in ROS detoxifying/repair genes were more susceptible to AGXX/aminoglycoside treatment. We further demonstrate that this synergistic interaction was associated with a significant increase in outer and inner membrane permeability, resulting in increased antibiotic influx. Our study also revealed that AGXX/aminoglycoside-mediated killing requires an active proton motive force across the bacterial membrane. Overall, our findings provide an understanding of cellular targets that could be inhibited to increase the activity of conventional antimicrobial

The effect of malting on phenolic compounds and radical scavenging activity in grains and breakfast cereals

Abstract: Breakfast cereals are popular grain foods and sources of polyphenols. Malting alters polyphenol content and activity; however, effects are varied. The total polyphenol content (TPC), radical scavenging activity (RSA), and polyphenol profile were analyzed in unmalted and malted grains (wheat, barley, and sorghum) and breakfast cereals (wheat, barley) by Folin Ciocalteu Reagent (FCR), % inhibition of the free radical 2,2-diphenyl-1-picryl-hydrazyl, and high performance liquid chromatography. Higher TPC was observed in all malted grains and breakfast cereals compared with unmalted samples (p < 0.05). Higher RSA was also observed in all malted samples compared to unmalted samples (p < 0.05) except for wheat grain to malted wheat grain. In this study, malting induced additional polyphenols and antioxidant activity in grains and cereal products. Malted grain breakfast cereals may be practical sources of polyphenol antioxidants. Practical Application: This study utilized malting in a unique way to investigate potential health benefits of polyphenols and antioxidant activity in grains (wheat, barley, and sorghum) and ready-to-eat breakfast cereals (wheat and barley). This study found that grains and breakfast cereals are important sources of antioxidant polyphenols, and these were significantly increased in malted varieties. Understanding this is important as grains and breakfast cereals are widely consumed staple foods. Consuming healthier grain products may be a practical strategy in reducing the risk of noncommunicable diseases such as colorectal cancer and type-2 diabetes, where wholegrain consumption may be important in prevention

Long-term consequences of the misuse of ivermectin data

Ivermectin is an oral anti-infective medicine that is integral to neglected tropical disease programmes. It is safe and effective for the treatment and control of lymphatic filariasis, scabies, and onchocerciasis, sometimes as part of a mass drug administration, as recognised in the WHO road map for neglected tropical diseases 2021–30. The WHO essential medicines list provides recommendations for minimum medicine needs for a basic health-care system, which includes ivermectin as an anthelmintic, antifilarial, and antiectoparasitic treatment. There has been a groundswell of opinion across several countries that ivermectin might be useful in reducing the symptoms of and mortality due to COVID-19, with many citing meta-analyses that infer positive effects; however, these conclusions appear to be unreliable

A randomised controlled trial assessing the use of citalopram, sertraline, fluoxetine and mirtazapine in preventing relapse in primary care patients who are taking long-term maintenance antidepressants (ANTLER : ANTidepressants to prevent reLapse in dEpRession): study protocol for a randomised controlled trial

BACKGROUND: Antidepressants are used both for treating acute episodes and for prophylaxis to prevent future episodes of depression, also called maintenance treatment. This article describes the protocol for a randomised controlled trial (ANTLER: ANTidepressants to prevent reLapse in dEpRession) to investigate the clinical effectiveness and cost-effectiveness in UK primary care of continuing on long-term maintenance antidepressants compared with a placebo in preventing relapse of depression in those who have taken antidepressants for more than 9 months and who are currently well enough to consider stopping maintenance treatment. METHODS/DESIGN: The ANTLER trial is an individually randomised, double-blind, placebo-controlled trial in which participants are randomised to remain on active medication or to take an identical placebo after a tapering period of 2 months. Eligible participants are those who: are between the ages of 18 and 74 years; have had at least two episodes of depression; and have been taking antidepressants for 9 months or more and are currently taking citalopram 20 mg, sertraline 100 mg, fluoxetine 20 mg or mirtazapine 30 mg but are well enough to consider stopping their medication. The participants will be followed up at 6, 12, 26, 39 and 52 weeks. The primary outcome will be the time in weeks to the beginning of the first episode of depression after randomisation. This will be measured using a retrospective version of the Clinical Interview Schedule-Revised administered at 12, 26, 39 and 52 weeks. Secondary outcomes will include depressive and anxiety symptoms, adverse effects, withdrawal symptoms, emotional processing tasks, quality of life and the resources and costs used. We will also perform a cost-effectiveness analysis based on results of the trial. DISCUSSION: The ANTLER trial findings will inform primary care prescribing practice by providing a valid and generalisable estimate of the clinical effectiveness and cost-effectiveness of long-term maintenance treatment with antidepressants in UK primary care. TRIAL REGISTRATION: Controlled Trials ISRCTN Registry, ISRCTN15969819. Registered on 21 September 2015