Developing an Active Canopy Sensor-Based Integrated Precision Rice Management System for Improving Grain Yield and Quality, Nitrogen Use Efficiency, and Lodging Resistance

Active crop sensor-based precision nitrogen (N) management can significantly improve N use efficiency but generally does not increase crop yield. The objective of this research was to develop and evaluate an active canopy sensor-based precision rice management system in terms of grain yield and quality, N use efficiency, and lodging resistance as compared with farmer practice, regional optimum rice management system recommended by the extension service, and a chlorophyll meter-based precision rice management system. Two field experiments were conducted from 2011 to 2013 at Jiansanjiang Experiment Station of China Agricultural University in Heilongjiang, China, involving four rice management systems and two varieties (Kongyu 131 and Longjing 21). The results indicated that the canopy sensor-based precision rice management system significantly increased rice grain yield (by 9.4–13.5%) over the farmer practice while improving N use efficiency, grain quality, and lodging resistance. Compared with the already optimized regional optimum rice management system, in the cool weather year of 2011, the developed system decreased the N rate applied in Kongyu 131 by 12% and improved N use efficiency without inducing yield loss. In the warm weather year of 2013, the canopy sensor-based management system recommended an 8% higher N rate to be applied in Longjing 21 than the regional optimum rice management, which improved rice panicle number per unit area and eventually led to increased grain yield by over 10% and improved N use efficiency. More studies are needed to further test the developed active canopy sensor-based precision rice management system under more diverse on-farm conditions and further improve it using unmanned aerial vehicle or satellite remote sensing technologies for large-scale applications.publishedVersio

Rice crop phenology mapping at high spatial and temporal resolution using downscaled MODIS time-series

Satellite data holds considerable potential as a source of information on rice crop growth which can be used to inform agronomy. However, given the typical field sizes in many rice-growing countries such as China, data from coarse spatial resolution satellite systems such as the Moderate Resolution Imaging Spectroradiometer (MODIS) are inadequate for resolving crop growth variability at the field scale. Nevertheless, systems such as MODIS do provide images with sufficient frequency to be able to capture the detail of rice crop growth trajectories throughout a growing season. In order to generate high spatial and temporal resolution data suitable for mapping rice crop phenology, this study fused MODIS data with lower frequency, higher spatial resolution Landsat data. An overall workflow was developed which began with image preprocessing, calculation of multi-temporal normalized difference vegetation index (NDVI) images, and spatiotemporal fusion of data from the two sensors. The Spatial and Temporal Adaptive Reflectance Fusion Model was used to effectively downscale the MODIS data to deliver a time-series of 30 m spatial resolution NDVI data at 8-day intervals throughout the rice-growing season. Zonal statistical analysis was used to extract NDVI time-series for individual fields and signal filtering was applied to the time-series to generate rice phenology curves. The downscaled MODIS NDVI products were able to characterize the development of paddy rice at fine spatial and temporal resolutions, across wide spatial extents over multiple growing seasons. These data permitted the extraction of key crop seasonality parameters that quantified inter-annual growth variability for a whole agricultural region and enabled mapping of the variability in crop performance between and within fields. Hence, this approach can provide rice crop growth data that is suitable for informing agronomic policy and practice across a wide range of scales

Mapping paddy rice fields by applying machine learning algorithms to multi-temporal Sentinel-1A and Landsat data

Sentinel-1A synthetic aperture radar (SAR) data present an opportunity for acquiring crop information without restrictions caused by weather and illumination conditions, at a spatial resolution appropriate for individual rice fields and a temporal resolution sufficient to capture the growth profiles of different crop species. This study investigated the use of multi-temporal Sentinel-1A SAR data and Landsat-derived normalized difference vegetation index (NDVI) data to map the spatial distribution of paddy rice fields across parts of the Sanjiang plain, in northeast China. The satellite sensor data were acquired throughout the rice crop-growing season (May–October). A co-registered set of 10 dual polarization (VH/VV) SAR and NDVI images depicting crop phenological development were used as inputs to Support Vector Machine (SVM) and Random Forest (RF) machine learning classification algorithms in order to map paddy rice fields. The results showed a significant increase in overall classification when the NDVI time-series data were integrated with the various combinations of multi-temporal polarization channels (i.e. VH, VV, and VH/VV). The highest classification accuracies overall (95.2%) and for paddy rice (96.7%) were generated using the RF algorithm applied to combined multi-temporal VH polarization and NDVI data. The SVM classifier was most effective when applied to the dual polarization (i.e. VH and VV) SAR data alone and this generated overall and paddy rice classification accuracies of 91.6% and 82.5%, respectively. The results demonstrate the practicality of implementing RF or SVM machine learning algorithms to produce 10 m spatial resolution maps of paddy rice fields with limited ground data using a combination of multi-temporal SAR and NDVI data, where available, or SAR data alone. The methodological framework developed in this study is apposite for large-scale implementation across China and other major rice-growing regions of the world

Fully Photonic Integrated Wearable Optical Interrogator

Wearable technology constitutes a pioneering and leading innovation and a market development platform worldwide for technologies worn close to the body. Wearable optical fiber sensors have the most value for advanced multiparameter sensing in digital health monitoring systems. We demonstrated the first example of a fully integrated optical interrogator. By integrating all the optical components on a silicon photonic chip, we realized a stable, miniaturized and low-cost optical interrogator for the continuous, dynamic, and long-term acquisition of human physiological signals. The interrogator was integrated in a wristband, enabling the detection of body temperature and heart sounds. Our study paves the way for the development of watch-sized integrated wearable optical interrogators with potential applications in health monitoring and can be directly exploited for the customized design of ultraminiaturized optical interrogator systems.H.L. acknowledges the support from the Tianjin Talent Special Support Program. J.D.P.G. acknowledges the support from the Serra Hunter Program, the ICREA Academia Program, and the Tianjin Distinguished University Professor Program. This work was supported by the National Natural Science Foundation of China (no. 61675154), the Tianjin Key Research and Development Program (no. 19YFZCSY00180), the Tianjin Major Project for Civil-Military Integration of Science and Technology (no. 18ZXJMTG00260), the Tianjin Science and Technology Program (no. 20YDTPJC01380), and the Tianjin Municipal Special Foundation for Key Cultivation of China (no. XB202007)

Corn Nitrogen Nutrition Index Prediction Improved by Integrating Genetic, Environmental, and Management Factors with Active Canopy Sensing Using Machine Learning

Accurate nitrogen (N) diagnosis early in the growing season across diverse soil, weather, and management conditions is challenging. Strategies using multi-source data are hypothesized to perform significantly better than approaches using crop sensing information alone. The objective of this study was to evaluate, across diverse environments, the potential for integrating genetic (e.g., comparative relative maturity and growing degree units to key developmental growth stages), environmental (e.g., soil and weather), and management (e.g., seeding rate, irrigation, previous crop, and preplant N rate) information with active canopy sensor data for improved corn N nutrition index (NNI) prediction using machine learning methods. Thirteen site-year corn (Zea mays L.) N rate experiments involving eight N treatments conducted in four US Midwest states in 2015 and 2016 were used for this study. A proximal RapidSCAN CS-45 active canopy sensor was used to collect corn canopy reflectance data around the V9 developmental growth stage. The utility of vegetation indices and ancillary data for predicting corn aboveground biomass, plant N concentration, plant N uptake, and NNI was evaluated using singular variable regression and machine learning methods. The results indicated that when the genetic, environmental, and management data were used together with the active canopy sensor data, corn N status indicators could be more reliably predicted either using support vector regression (R2 = 0.74–0.90 for prediction) or random forest regression models (R2 = 0.84–0.93 for prediction), as compared with using the best-performing single vegetation index or using a normalized difference vegetation index (NDVI) and normalized difference red edge (NDRE) together (R2 \u3c 0.30). The N diagnostic accuracy based on the NNI was 87% using the data fusion approach with random forest regression (kappa statistic = 0.75), which was better than the result of a support vector regression model using the same inputs. The NDRE index was consistently ranked as the most important variable for predicting all the four corn N status indicators, followed by the preplant N rate. It is concluded that incorporating genetic, environmental, and management information with canopy sensing data can significantly improve in-season corn N status prediction and diagnosis across diverse soil and weather conditions

Corn Nitrogen Nutrition Index Prediction Improved by Integrating Genetic, Environmental, and Management Factors with Active Canopy Sensing Using Machine Learning

Accurate nitrogen (N) diagnosis early in the growing season across diverse soil, weather, and management conditions is challenging. Strategies using multi-source data are hypothesized to perform significantly better than approaches using crop sensing information alone. The objective of this study was to evaluate, across diverse environments, the potential for integrating genetic (e.g., comparative relative maturity and growing degree units to key developmental growth stages), environmental (e.g., soil and weather), and management (e.g., seeding rate, irrigation, previous crop, and preplant N rate) information with active canopy sensor data for improved corn N nutrition index (NNI) prediction using machine learning methods. Thirteen site-year corn (Zea mays L.) N rate experiments involving eight N treatments conducted in four US Midwest states in 2015 and 2016 were used for this study. A proximal RapidSCAN CS-45 active canopy sensor was used to collect corn canopy reflectance data around the V9 developmental growth stage. The utility of vegetation indices and ancillary data for predicting corn aboveground biomass, plant N concentration, plant N uptake, and NNI was evaluated using singular variable regression and machine learning methods. The results indicated that when the genetic, environmental, and management data were used together with the active canopy sensor data, corn N status indicators could be more reliably predicted either using support vector regression (R2 = 0.74–0.90 for prediction) or random forest regression models (R2 = 0.84–0.93 for prediction), as compared with using the best-performing single vegetation index or using a normalized difference vegetation index (NDVI) and normalized difference red edge (NDRE) together (R2 \u3c 0.30). The N diagnostic accuracy based on the NNI was 87% using the data fusion approach with random forest regression (kappa statistic = 0.75), which was better than the result of a support vector regression model using the same inputs. The NDRE index was consistently ranked as the most important variable for predicting all the four corn N status indicators, followed by the preplant N rate. It is concluded that incorporating genetic, environmental, and management information with canopy sensing data can significantly improve in-season corn N status prediction and diagnosis across diverse soil and weather conditions

mammalian target of rapamycin complex 2 controls folding and stability of Akt and protein kinase C

The target of rapamycin (TOR), as part of the rapamycinsensitive TOR complex 1 (TORC1), regulates various aspects of protein synthesis. Whether TOR functions in this process as part of TORC2 remains to be elucidated. Here, we demonstrate that mTOR, SIN1 and rictor, components of mammalian (m)TORC2, are required for phosphorylation of Akt and conventional protein kinase C (PKC) at the turn motif (TM) site. This TORC2 function is growth factor independent and conserved from yeast to mammals. TM site phosphorylation facilitates carboxyl-terminal folding and stabilizes newly synthesized Akt and PKC by interacting with conserved basic residues in the kinase domain. Without TM site phosphorylation, Akt becomes protected by the molecular chaperone Hsp90 from ubiquitination-mediated proteasome degradation. Finally, we demonstrate that mTORC2 independently controls the Akt TM and HM sites in vivo and can directly phosphorylate both sites in vitro. Our studies uncover a novel function of the TOR pathway in regulating protein folding and stability, processes that are most likely linked to the functions of TOR in protein synthesis

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data