Monoclonal antibodies against sporangia and spores of Marteilla sp. (Protozoa: Ascetospora)

6 pages, 4 figures.Digestive glands of mussels Mytilus edulis from Brittany, France, infected with Marteilia sp. (Ascetospora) were used to purify the parasite. A modification of a previously used punfication protocol increased purification efficiency, permitting sporangial primordia and sporangia of Marteilia sp. to be obtained. Mouse (Balb/c) monoclonal antibodies were generated against this parasite. From the fusion, 26 monoclonal antibodies against Marteilia sp. were obtained. Antibodies from 6 clones reacted only with Marteilia sp. cells and not with normal host tissues. Four of these antibodies (1/1-3, 3/1-1, 4/1-1 and 6/2-3) reacted with the sporangia wall and two with the spore cytoplasm (9/1-1 and 12/5-1), Antibodies cross-reacted with Marteilia refringens from Mytilus galloprovincialis obtained in the Ría de Vigo, Spain.J.A.F.R. acknowledges Xunta de Galicia, Spain, for his with research fellowship at the IIM-CSIC and Caixa Galicia for his research fellowship at IFREMER - La Tremblade.Peer reviewe

Surface Recombination Via Interface Defects in Field Effect Transistors

Recombination current at the oxide-semiconductor interface of metal-oxide-semiconductor devices has been analyzed and compared with the experimental result. The activity of interface traps is dependent on the energy level and on the operating conditions. A model is shown to be powerful to describe the effect of energy level of bulk recombination centers on the values of reverse recombination current

Bioremediation of water recirculation in white shrimp culture using autochthonous microbiota of red mangrove

La actividad acuícola en el cultivo del camarón blanco utilizando agua recirculante deteriora la calidad del agua por la acción hidrodinámica aguametabolitos- materia orgánica, que generan impactos negativos en la producción del crustáceo. El objetivo de esta investigación es demostrar que la microbiota autóctona extraída del mangle rojo estimula la biorremediación. Para este fin se sometió la microbiota a un proceso de bioaumentación que enriquece su biodiversidad, de tal forma que al ser utilizada garantice la actividad biorremediadora. La aplicación del tratamiento se inicia con el diagnóstico de calidad del agua en las diferentes etapas del proceso, a través del monitoreo de compuestos tóxicos como, amonio, nitritos y nitratos y la evaluación de la biodiversidad microbiana utilizando técnicas metagenómicas como la Prueba de Reacción de la Polimerasa, Electroforesis en la comprobación del ADN y su secuenciación mediante la técnica ILLUMINA. Los resultados revelan que los parámetros tóxicos mantienen niveles óptimos de calidad con la aplicación de la microbiota compuesta por 1780 especies microbianas. Estadísticamente se demostró la disminución significativa de niveles de compuestos tóxicos que comprueba la hipótesis, de que la microbiota del mangle rojo actúa eficazmente en la biorremediación del agua en el cultivo de camarón blanco.The aquaculture activity in the white shrimp culture using recirculating water deteriorates the quality of the water by the hydrodynamic action of watermetabolites- organic matter, which generate negative impacts on the production of the crustacean. The objective of this research is to demonstrate that the autochthonous microbiota extracted from the red mangrove stimulates bioremediation. For this purpose, the microbiota was subjected to a process of bioaugmentation that enriches its biodiversity, so that when used, it guarantees bioremediation activity. The application of the treatment begins with the diagnosis of water quality in the different stages of the process, through the monitoring of toxic compounds such as ammonium, nitrites and nitrates and the evaluation of microbial biodiversity using metagenomic techniques such as the Reaction Test of the Polymerase, Electrophoresis in DNA testing and sequencing using the ILLUMINA technique. The results reveal that the toxic parameters maintain optimum levels of quality with the application of the microbiota composed of 1780 microbial species. Statistically it was demonstrated the significant decrease of levels of toxic compounds that the hypothesis proves, that the red mangrove microbiota acts efficiently in the bioremediation of water in the white shrimp culture

Diode Parameter Determination Applied to LDD-MOSFETs for Device Characterization

The electrical properties of the drain-substrate diode of MOSFETs are shown to be related to the device geometrical structure. The two dimensional analysis takes into account the edge effects of the length and width of the gate. Intrinsic parameters are extracted from current-voltage characteristics and obtained dependent on these dimensions

SEIS: Insight's Seismic Experiment for Internal Structure of Mars.

By the end of 2018, 42 years after the landing of the two Viking seismometers on Mars, InSight will deploy onto Mars' surface the SEIS (Seismic Experiment for Internal Structure) instrument; a six-axes seismometer equipped with both a long-period three-axes Very Broad Band (VBB) instrument and a three-axes short-period (SP) instrument. These six sensors will cover a broad range of the seismic bandwidth, from 0.01 Hz to 50 Hz, with possible extension to longer periods. Data will be transmitted in the form of three continuous VBB components at 2 sample per second (sps), an estimation of the short period energy content from the SP at 1 sps and a continuous compound VBB/SP vertical axis at 10 sps. The continuous streams will be augmented by requested event data with sample rates from 20 to 100 sps. SEIS will improve upon the existing resolution of Viking's Mars seismic monitoring by a factor of ∼ 2500 at 1 Hz and ∼ 200 000 at 0.1 Hz. An additional major improvement is that, contrary to Viking, the seismometers will be deployed via a robotic arm directly onto Mars' surface and will be protected against temperature and wind by highly efficient thermal and wind shielding. Based on existing knowledge of Mars, it is reasonable to infer a moment magnitude detection threshold of M w ∼ 3 at 40 ∘ epicentral distance and a potential to detect several tens of quakes and about five impacts per year. In this paper, we first describe the science goals of the experiment and the rationale used to define its requirements. We then provide a detailed description of the hardware, from the sensors to the deployment system and associated performance, including transfer functions of the seismic sensors and temperature sensors. We conclude by describing the experiment ground segment, including data processing services, outreach and education networks and provide a description of the format to be used for future data distribution.Electronic supplementary materialThe online version of this article (10.1007/s11214-018-0574-6) contains supplementary material, which is available to authorized users

Transcriptome characterization of the South African abalone Haliotis midae using sequencing-by-synthesis

<p>Abstract</p> <p>Background</p> <p>Worldwide, the genus <it>Haliotis </it>is represented by 56 extant species and several of these are commercially cultured. Among the six abalone species found in South Africa, <it>Haliotis midae </it>is the only aquacultured species. Despite its economic importance, genomic sequence resources for <it>H. midae</it>, and for abalone in general, are still scarce. Next generation sequencing technologies provide a fast and efficient tool to generate large sequence collections that can be used to characterize the transcriptome and identify expressed genes associated with economically important traits like growth and disease resistance.</p> <p>Results</p> <p>More than 25 million short reads generated by the Illumina Genome Analyzer were <it>de novo </it>assembled in 22,761 contigs with an average size of 260 bp. With a stringent <it>E</it>-value threshold of 10^-10, 3,841 contigs (16.8%) had a BLAST homologous match against the Genbank non-redundant (NR) protein database. Most of these sequences were annotated using the gene ontology (GO) and eukaryotic orthologous groups of proteins (KOG) databases and assigned to various functional categories. According to annotation results, many gene families involved in immune response were identified. Thousands of simple sequence repeats (SSR) and single nucleotide polymorphisms (SNP) were detected. Setting stringent parameters to ensure a high probability of amplification, 420 primer pairs in 181 contigs containing SSR loci were designed.</p> <p>Conclusion</p> <p>This data represents the most comprehensive genomic resource for the South African abalone <it>H. midae </it>to date. The amount of assembled sequences demonstrated the utility of the Illumina sequencing technology in the transcriptome characterization of a non-model species. It allowed the development of several markers and the identification of promising candidate genes for future studies on population and functional genomics in <it>H. midae </it>and in other abalone species.</p