An attempt to use color as a tool for high resolution correlations between estuarine sedimentary cores from Algarve (Portugal)

Nowadays, paleoenvironmental and climatological researches focus on studying shortterm climatic changes and one of the more sensitive environments for recording those short-term climatic variations are the high sedimentation rate of coastal regions. Accordingly, 4 estuaries from the Algarve region, south of Portugal, have been cored in order to study sedimentary and climatic variations during recent times (c.a. 5000 yrs). For this purpose, we applied sampling and analytical techniques from deep-sea studies, allowing the acquirement of almost continuous data profiles, as the color data obtained with the spectrophotometer Colortron

Correlation of estuarine sedimentary cores based on color analysis: an example from the Algarve region (Portugal)

Coastal zones play a significant role in the regional development and have been an important area for human occupation through time. The study of estuarine sediments applied to sea level changes had always been of extreme importance, as it reflects the coastal line evolution, either by local, regional or global changes

Is it possible to use "twin cores" as a unique sedimentary record? An experimental design based on sediment color

Sedimentary cores are widely used for studying Quaternary records. However, the amount of sediment that is available is proportional to the diameter of the core, which is rarely bigger than 15 cm. One way to obtain more sediment is to use two cores retrieved from almost the same location and use them as if they represent a unique sedimentary record. In the present work, an experimental design has been applied to verify if “twin cores” from an estuary can be considered as representing the same sedimentary record with twice the amount of sediment to study. Because sediment can be characterized based on its color, the variables used as replicates in the experimental design are the three Lab CIE colors acquired with a X-Rite Colortron spectrophotometer. Sediment cores were retrieved from the upper saltmarsh of Gilão River’s estuary, southern Portugal. Twin cores, with in between distances of 50 cm, 100 cm and 200 cm, from two different sites were analysed. Results from a nested ANOVA show that even for the closest twin cores (50 cm apart) there is at least one color variable that shows significant variations between the profiles of both cores. These results clearly show that “twin cores” cannot be used as a unique sedimentary record without any previous testing, at least in such transitional regions

Regional sea level change study based on estuarine sediment color and biogeochemistry - preliminary results

Coastal zones play a significant role in the regional development and have been an important area for human occupation through time. The study of estuarine sediments applied to sea level changes had always been of extreme importance, as it reflects the coastal line evolution, either by local, regional or global changes

Impacts of the combined exposure to seawater acidification and arsenic on the proteome of Crassostrea angulata and Crassostrea gigas

Proteomic analysis was performed to compare the effects of Arsenic (As), seawater acidification (Low pH) and the combination of both stressors (Low pH + As) on Crassostrea angulata and Crassostrea gigas juveniles in the context of global environmental change. This study aimed to elucidate if two closely related Crassostrea species respond similarly to these environmental stressors, considering both single and combined exposures, to infer if the simultaneous exposure to both stressors induced a differentiated response. Identification of the most important differentially expressed proteins between conditions revealed marked differences in the response of each species towards single and combined exposures, evidencing species-related differences towards each experimental condition. Moreover, protein alterations observed in the combined exposure (Low pH + As) were substantially different from those observed in single exposures. Identified proteins and their putative biological functions revealed an array of modes of action in each condition. Among the most important, those involved in cellular structure (Actin, Atlastin, Severin, Gelsolin, Coronin) and extracellular matrix modulation (Ependymin, Tight junction ZO-1, Neprilysin) were strongly regulated, although in different exposure conditions and species. Data also revealed differences regarding metabolic modulation capacity (ATP β, Enolase, Aconitate hydratase) and oxidative stress response (Aldehyde dehydrogenase, Lactoylglutathione, Retinal dehydrogenase) of each species, which also depended on single or combined exposures, illustrating a different response capacity of both oyster species to the presence of multiple stressors. Interestingly, alterations of piRNA abundance in C. angulata suggested genome reconfiguration in response to multiple stressors, likely an important mode of action related to adaptive evolution mechanisms previously unknown to oyster species, which requires further investigation. The present findings provide a deeper insight into the complexity of C. angulata and C. gigas responses to environmental stress at the proteome level, evidencing different capacities to endure abiotic changes, with relevance regarding the ecophysiological fitness of each species and competitive advantages in a changing environment.Centro-01-0145-FEDER-000018info:eu-repo/semantics/publishedVersio

Algarve: a study case for global vs regional climatic changes in mid latitude estuaries during the Holocene

Located at the extreme southwestern end of the Iberian Peninsula, in the South of Portugal, the Algarve province is bordered to the north by the Alentejo province, to the east by the Guadiana River, and to the west and south by the Atlantic Ocean. Its coastal region can be divided into two different sectors: the eastern sector, which is mainly made of barrier island and lagoon system named the Ria Formosa, and the western sector mainly represented by clastic and carbonated cliffs. In each sector, we sampled long and short sedimentary cores (ca. 30m and 3m, respectively) in two different estuaries. Therefore, the different sedimentary records obtained from the four estuaries should permit to identify global vs. west-eastern regional climatic changes through the observation of several proxies. Two of these proxies are sediment color and magnetic susceptibility, which have been acquired every five centimeters along core depth, using the Colortron II spectrophotometer and SM-20 magneto-susceptometer, respectively. Sediment surfaces of short cores were also digitized using the Mustek 1200 A3 PRO Scanner. Furthermore, short cores have been sampled every five centimeters along depth in order to analyze the sediment for its major and minor elements content, its organic carbon content, and its granulometry and mineralogy. Some shell fragments have also been sampled and sent for radiocarbon radiometry or AMS dating, allowing therefore constructing some absolute chronologies for the several cores. Some relative chronologies based on digital color profiles are also being used in order to correlate sedimentary sequences between cores. All the cores are mainly composed of clay, with the existence of soil horizons at the top of the cores and frequent sand layers towards their base. Several element profiles along depths are compared, namely those regarding Sr, Ca, F, Ba, Al and Ti, as well as their ratios. First results indicate that geochemical and color data allow to identify similar sequences from west to east probably related to major climate episodes. Although, pronounced differences in mineralogy and accumulation rates from on site to another are probably due to differences, respectively, in the geochemistry of the drainage basin substratum and in the flow rate of the rivers, and therefore to local precipitation. We acknowledge FEDER and OE that financed this study through the Portuguese Foundation for Science and Technology (FCT) (POCTI/CTA/39733/2001)

An attempt to develop spectrophotometry as a proxy for sedimentary studies in estuarine environments

In deep-sea sediment studies, reflectance has been used as a proxy for high vs. low productivity intervals for several years. This value of the white-black scale is obtained through the use of spectrophotometers when using the CIE Lab color system, which also gives values on green-red and blue-yellow scales. In estuarine/continental sediment studies, color data are still often based on Munsell Table which does not allow to obtain numeric and continuous data and which is biased by the observer’s choice

Microplastics effects in Scrobicularia plana

One of the most common plastics in the marine environment is polystyrene (PS) that can be broken down to micro sized particles. Marine organisms are vulnerable to the exposure to microplastics. This study assesses the effects of PS microplastics in tissues of the clam Scrobicularia plana. Clams were exposed to 1mgL-1(20μm) for 14days, followed by 7days of depuration. A qualitative analysis by infrared spectroscopy in diffuse reflectance mode period detected the presence of microplastics in clam tissues upon exposure, which were not eliminated after depuration. The effects of microplastics were assessed by a battery of biomarkers and results revealed that microplastics induce effects on antioxidant capacity, DNA damage, neurotoxicity and oxidative damage. S. plana is a significant target to assess the environmental risk of PS microplastics.info:eu-repo/semantics/publishedVersio

Influence of particle size on ecotoxicity of low-density polyethylene microplastics, with and without adsorbed benzo-a-pyrene, in Clam Scrobicularia plana

This study investigated the ecotoxicological effects of differently sized (4–6 µm and 20–25 µm) low-density polyethylene (LDPE) microplastics (MPs), with and without adsorbed benzo-a-pyrene (BaP), in clam Scrobicularia plana. Biomarkers of oxidative stress (superoxide dismutase—SOD; catalase—CAT), biotransformation (glutathione-S-transferases—GST), oxidative damage (lipid peroxidation—LPO) and neurotoxicity (acetylcholinesterase—AChE) were analysed in gills and digestive glands at different time intervals for a total of 14 days of exposure. In order to have a better impact perspective of these contaminants, an integrated biomarker response index (IBR) and Health Index were applied. Biomarker alterations are apparently more related to smaller sized (4–6 µm) MPs in gills and to virgin LDPE MPs in the digestive gland according to IBR results, while the digestive gland was more affected by these MPs according to the health index.info:eu-repo/semantics/publishedVersio

Environmental hazard assessment of a marine mine tailings deposit site and potential implications for deep-sea mining

Portmán Bay is a heavily contaminated area resulting from decades of metal mine tailings disposal, and is considered a suitable shallow-water analogue to investigate the potential ecotoxicological impact of deep-sea mining. Resuspension plumes were artificially created by removing the top layer of the mine tailings deposit by bottom trawling. Mussels were deployed at three sites: i) off the mine tailings deposit area; ii) on the mine tailings deposit beyond the influence from the resuspension plumes; iii) under the influence of the artificially generated resuspension plumes. Surface sediment samples were collected at the same sites for metal analysis and ecotoxicity assessment. Metal concentrations and a battery of biomarkers (oxidative stress, metal exposure, biotransformation and oxidative damage) were measured in different mussel tissues. The environmental hazard posed by the resuspension plumes was investigated by a quantitative weight of evidence (WOE) model that integrated all the data. The resuspension of sediments loaded with metal mine tails demonstrated that chemical contaminants were released by trawling subsequently inducing ecotoxicological impact in mussels' health. Considering as sediment quality guidelines (SQGs) those indicated in Spanish action level B for the disposal of dredged material at sea, the WOE model indicates that the hazard is slight off the mine tailings deposit, moderate on the mine tailings deposit without the influence from the resuspension plumes, and major under the influence of the resuspension plumes. Portmán Bay mine tailings deposit is a by-product of sulphide mining, and despite differences in environmental setting, it can reflect the potential ecotoxic effects to marine fauna from the impact of resuspension of plumes created by deep-sea mining of polymetallic sulphides. A similar approach as in this study could be applied in other areas affected by sediment resuspension and for testing future deep-sea mining sites in order to assess the associated environmental hazards.info:eu-repo/semantics/publishedVersio