Evaluation of fine root length and diameter measurements obtained using RHIZO image analysis

Image analysis systems facilitate rapid measurement of root length and diameter, but their accuracy is not easily determined. The objective of this study was to develop a set of simple experiments for evaluating the accuracy of fine root measurements obtained using image analysis. Using the system RHIZO (trademark of Regent Instruments, Quebec), we tested the accuracy of (i) length measurements made over a range of root lengths per unit area, (ii) average diameter measurements and length per diameter distributions in string, wire, and fine root samples of varying diameter, and (iii) diameter measurements on short segments of diagonally oriented objects. Our results suggest that preliminary testing of image analysis systems is absolutely necessary for producing reliable root measurements. Total length was accurately determined for typically encountered length per unit areas of <1.5cm cm-2. For samples with higher values, however, the method underestimated total length by >5%. It is therefore recommended that users of image analysis systems determine this maximum length per unit area for accurate determinations of total root length. In samples that contained different string diameters, the total sample length and average string diameter could accurately be measured. However, the length per diameter class was underestimated by >20% when the string diameter was less than one pixel smaller than the upper limit of the diameter class. Adjustment of diameter intervals and increasing the scanner resolution are required to reduce this underestimation. Both the length and the angle of the short segments analyzed were found to influence diameter measurements. Similar sets of experiments are proposed for a rigorous evaluation of the performance of other image analysis systems on root measurements

Effect of branch position and light availability on shoot growth of understory sugar maple and yellow birch saplings

Phenotypic plasticity enables tree saplings to change their morphology according to their environment to grow toward a better light micro-habitat. Therefore, processes of crown development could be expected to vary as a function of light. The objectives of this study were to (i) evaluate the effects of position and light availability on shoot growth within the crowns of understory saplings of sugar maple (Acer saccharum Marsh.) and yellow birch (Betula alleghaniensis Britton.); (ii) develop a new vigour index for shoots; and (iii) evaluate the possible factors relating to branch mortality in the crown of sugar maple saplings. The results revealed that there is a clear branch position effect on shoot growth in the crown for yellow birch saplings and that it is partly related to the presence of two types of shoots. Dead branches were located at the bottom of the crown of sugar maple saplings; they were smaller in size, had wider angles and had lower indexes of vigour than live branches found nearby. Preliminary results obtained on the vigour index indicate that it is a potentially useful tool for predicting the growth and vigour status of a branch

Oxynitride glass fibers

Research at the Army Materials Technology Laboratory (AMTL) and elsewhere has shown that many glass properties including elastic modulus, hardness, and corrosion resistance are improved markedly by the substitution of nitrogen for oxygen in the glass structure. Oxynitride glasses, therefore, offer exciting opportunities for making high modulus, high strength fibers. Processes for making oxynitride glasses and fibers of glass compositions similar to commercial oxide glasses, but with considerable enhanced properties, are discussed. We have made glasses with elastic moduli as high as 140 GPa and fibers with moduli of 120 GPa and tensile strengths up to 2900 MPa. AMTL holds a U.S. patent on oxynitride glass fibers, and this presentation discusses a unique process for drawing small diameter oxynitride glass fibers at high drawing rates. Fibers are drawn through a nozzle from molten glass in a molybdenum crucible at 1550 C. The crucible is situated in a furnace chamber in flowing nitrogen, and the fiber is wound in air outside of the chamber, making the process straightforward and commercially feasible. Strengths were considerably improved by improving glass quality to minimize internal defects. Though the fiber strengths were comparable with oxide fibers, work is currently in progress to further improve the elastic modulus and strength of fibers. The high elastic modulus of oxynitride glasses indicate their potential for making fibers with tensile strengths surpassing any oxide glass fibers, and we hope to realize that potential in the near future

Blending of nanoscale and microscale in uniform large-area sculptured thin-film architectures

The combination of large thickness ($>3$ $\mu$m), large--area uniformity (75 mm diameter), high growth rate (up to 0.4 $\mu$m/min) in assemblies of complex--shaped nanowires on lithographically defined patterns has been achieved for the first time. The nanoscale and the microscale have thus been blended together in sculptured thin films with transverse architectures. SiO$_x$ ($x\approx 2$) nanowires were grown by electron--beam evaporation onto silicon substrates both with and without photoresist lines (1--D arrays) and checkerboard (2--D arrays) patterns. Atomic self--shadowing due to oblique--angle deposition enables the nanowires to grow continuously, to change direction abruptly, and to maintain constant cross--sectional diameter. The selective growth of nanowire assemblies on the top surfaces of both 1--D and 2--D arrays can be understood and predicted using simple geometrical shadowing equations.Comment: 17 pages, 9 figure

Neutrinos with Lorentz-violating operators of arbitrary dimension

The behavior of fermions in the presence of Lorentz and CPT violation is studied. Allowing for operators of any mass dimension, we classify all Lorentz-violating terms in the quadratic Lagrange density for free fermions. The result is adapted to obtain the effective hamiltonian describing the propagation and mixing of three flavors of left-handed neutrinos in the presence of Lorentz violation involving operators of arbitrary mass dimension. A characterization of the neutrino coefficients for Lorentz violation is provided via a decomposition using spin-weighted spherical harmonics. The restriction of the general theory to various special cases is discussed, including among others the renormalizable limit, the massless scenario, flavor-blind and oscillation-free models, the diagonalizable case, and several isotropic limits. The formalism is combined with existing data on neutrino oscillations and kinematics to extract a variety of measures of coefficients for Lorentz and CPT violation. For oscillations, we use results from the short-baseline experiments LSND and MiniBooNE to obtain explicit sensitivities to effects from flavor-mixing Lorentz-violating operators up to mass dimension 10, and we present methods to analyze data from long-baseline experiments. For propagation, we use time-of-flight measurements from the supernova SN1987A and from a variety of experiments including MINOS and OPERA to constrain oscillation-free Lorentz-violating operators up to mass dimension 10, and we discuss constraints from threshold effects in meson decays and Cherenkov emission.Comment: 35 pages two-column REVTe

Probing Relativity using Space-Based Experiments

An overview of space tests searching for small deviations from special relativity arising at the Planck scale is given. Potential high-sensitivity space-based experiments include ones with atomic clocks, masers, and electromagnetic cavities. We show that a significant portion of the coefficient space in the Standard-Model Extension, a framework that covers the full spectrum of possible effects, can be accessed using space tests. Some remarks on Lorentz violation in the gravitational sector are also given.Comment: 12 pages, invited talk at International Workshop, From Quantum to Cosmos, Warrenton, VA, USA, May 22-24, 200